CN214974003U

CN214974003U - Liposome prepares instrument fast

Info

Publication number: CN214974003U
Application number: CN202121237428.XU
Authority: CN
Inventors: 贾轶; 李春; 张小楠
Original assignee: Shanghai Puping Biotechnology Co ltd
Current assignee: Shanghai Puping Biotechnology Co ltd
Priority date: 2021-06-04
Filing date: 2021-06-04
Publication date: 2021-12-03
Anticipated expiration: 2031-06-04

Abstract

The utility model discloses a liposome prepares instrument fast, including preparing the jar, one side of preparation jar is equipped with the storage tank, be equipped with the fixed plate on the outer wall of preparation jar, electronic telescopic cylinder is installed to the fixed plate bottom, electronic telescopic cylinder below is equipped with the injection tube, be equipped with the push-and-pull rod in the injection tube, the push-and-pull rod bottom is equipped with the piston, the injection tube below is equipped with the liquid suction pipe, just preparation jar includes the outer jar of body and the inner tank body, is equipped with the heating chamber between the outer jar of body and the inner tank body, the heating intracavity is equipped with spiral heating coil, the inboard of the inner tank body is equipped with the puddler, be equipped with multiunit paddle stirring leaf on the outer wall of puddler. The utility model discloses an electronic telescoping cylinder control push-and-pull rod moves down, makes in the liquid in the injection tube can pour into the preparation jar at the uniform velocity into, easy operation, conveniently controls the injection volume of oil phase, and labour saving and time saving can improve the preparation efficiency of liposome greatly, and the error is little, can improve the quality of product.

Description

Liposome prepares instrument fast

Technical Field

The utility model relates to a liposome preparation equipment field, concretely relates to liposome prepares instrument fast.

Background

The liposome is an artificial membrane, the hydrophilic head of phospholipid molecule in water is inserted into water, the hydrophobic tail of the liposome extends to air, and the liposome is stirred to form a spherical liposome with double-layer lipid molecules, the diameter of the liposome is different from 25-1000 nm, the liposome can be used for transgene or prepared drugs, the drugs are delivered into cells by utilizing the characteristic that the liposome can be fused with cell membranes, the liposome preparation generally adopts the injection method, the film dispersion method, the ultrasonic dispersion method, the reverse evaporation method and other methods to prepare the liposome, when the liposome is prepared by adopting the injection method, lipid and fat-soluble drugs are dissolved in an organic solvent (oil phase), then the oil phase is injected into a water phase (containing water-soluble drugs) at uniform speed, the organic solvent is stirred to volatilize the organic solvent, and then the liposome is obtained by homogenization or ultrasound, and the ethanol injection method and the ether injection method can be divided according to the difference of the solvents.

The existing liposome preparation equipment usually uses an injector to manually inject the oil phase into the water phase at a uniform speed when the injection method is adopted to prepare the liposome, and the oil phase is injected by adopting the method, so that the injection amount of the oil phase needs to be noticed all the time, time and labor are wasted, the preparation efficiency is low, larger errors exist, and the quality of the solution after reaction is poor.

Therefore, it is necessary to invent a rapid liposome preparation apparatus to solve the above problems.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a liposome prepares instrument fast to solve liposome preparation equipment and use the syringe to manually inject the even speed of oil phase into water phase when adopting the injection method to prepare the liposome, adopt this kind of mode to inject the oil phase, not only need pay attention to the injection volume of oil phase constantly, waste time and energy, preparation inefficiency, and there is great error, make the not good problem of solution quality after the reaction easily.

In order to achieve the above object, the present invention provides the following technical solutions: a liposome rapid preparation instrument comprises a preparation tank, wherein a storage tank is arranged on one side of the preparation tank, a fixed plate is arranged on the outer wall of the preparation tank, an electric telescopic cylinder is vertically arranged at the bottom of the fixed plate, an injection cylinder is arranged below the electric telescopic cylinder, a push-pull rod is arranged in the injection cylinder, the top of the push-pull rod is fixedly connected with a piston rod of the electric telescopic cylinder, a piston is arranged at the bottom of the piston, a liquid suction pipe is arranged below the injection cylinder, the bottom end of the liquid suction pipe penetrates through the storage tank and extends to the inner side of the storage tank, a first throttle valve is arranged on the outer wall of the liquid suction pipe, a liquid conveying pipe is arranged above the first throttle valve, a second throttle valve is arranged on the outer wall of the liquid conveying pipe, two ends of the liquid conveying pipe are respectively communicated with the preparation tank and the liquid suction pipe, the preparation tank comprises an outer tank body and an inner tank body, a heating cavity is arranged between the outer tank body and the inner tank body, the heating intracavity is equipped with spiral heating coil, just the inboard of the inner tank body is equipped with the puddler, radially be equipped with multiunit paddle formula stirring leaf on the outer wall of puddler, just outer jar of body top is equipped with the motor, the output shaft of motor runs through the roof and the puddler top end fixed connection of the outer jar of body.

Preferably, the rear of preparation jar is equipped with the collecting vessel, the top of collecting vessel is equipped with the blast pipe, the both ends of blast pipe are linked together with preparation jar and collecting vessel respectively, just the control valve is installed to the lower part of blast pipe.

Preferably, a pressure relief pipe is installed at the top of the collecting tank, and a pressure relief valve is installed on the outer wall of the pressure relief pipe.

Preferably, a water heater is installed on the outer wall of the preparation tank through a hoop, a water outlet of the water heater is connected with a water inlet of the spiral heating coil through a pipeline, and a water inlet of the water heater is connected with a circulating water pump through a pipeline.

Preferably, one side of the preparation tank far away from the storage tank is provided with a water storage tank, and the water storage tank is respectively connected with a water inlet of the circulating water pump and a water outlet of the spiral heating coil pipe through pipelines.

Preferably, the top of the preparation tank and the top of the storage tank are both provided with feed inlets, and the feed inlets are in one of a circular shape, a square shape and a rectangular shape.

In the technical scheme, the utility model provides a technological effect and advantage:

1. the hot water which circularly flows in the spiral heating coil pipe is used for heating the liquid, the push-pull rod is controlled to move upwards by the electric telescopic cylinder, the first throttle valve is opened, the second throttle valve is closed, the liquid in the storage tank can enter the injection tube under the action of negative pressure, then the first throttle valve is closed, the second throttle valve is opened, the push-pull rod is controlled to move downwards by the electric telescopic cylinder, the liquid in the injection tube is injected into the preparation tank at a constant speed, then the stirring rod is controlled by the motor to drive a plurality of groups of paddle type stirring blades to rotate for stirring the liquid, and after ethanol or ether in the liquid is removed, the preparation of the liposome can be completed, the operation is simple, the injection amount of the oil phase is conveniently controlled, the time and the labor are saved, the preparation efficiency of the liposome can be greatly improved, the error is small, and the quality of the liposome can be improved;

2. water in the water storage tank is sent to the water heater through the circulating water pump to be heated, then the heated water can enter the spiral heating coil, and hot water circularly flows in the spiral heating coil through the arranged circulating water pump, so that the reutilization of water resources is realized;

3. through injecting cold water into in the collection tank, form low temperature environment, open the control valve, make in ethanol or ether gas gets into the collection tank, in low temperature environment, ethanol or ether gas can liquefy to realized the collection of ethanol or ether, the person of facilitating the use later stage is recycled ethanol or ether.

Drawings

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present invention, and other drawings can be obtained by those skilled in the art according to these drawings.

Fig. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a side cross-sectional view of the preparation tank of the present invention;

FIG. 3 is a side cross-sectional view of the syringe of the present invention;

fig. 4 is an enlarged view of a portion a in fig. 1 according to the present invention.

Description of reference numerals:

1. preparing a tank; 11. an outer tank body; 12. an inner tank body; 13. a motor; 14. a heating cavity; 15. a helical heating coil; 16. a stirring rod; 161. paddle type stirring blades; 2. an injection tube; 21. a push-pull rod; 22. a piston; 23. a liquid pumping pipe; 231. a first throttle valve; 24. a transfusion tube; 3. an electric telescopic cylinder; 31. a fixing plate; 4. a material storage tank; 5. a water storage tank; 6. a water heater; 7. a collection tank; 71. an exhaust pipe; 711. a control valve; 72. a pressure relief pipe; 721. a pressure relief valve; 8. a water circulating pump; 9. a feed inlet.

Detailed Description

In order to make the technical solution of the present invention better understood by those skilled in the art, the present invention will be further described in detail with reference to the accompanying drawings.

The utility model provides a liposome fast preparation instrument as shown in figures 1-4, which comprises a preparation tank 1, one side of the preparation tank 1 is provided with a storage tank 4, the outer wall of the preparation tank 1 is provided with a fixed plate 31, the bottom of the fixed plate 31 is vertically provided with an electric telescopic cylinder 3, the lower part of the electric telescopic cylinder 3 is provided with an injection tube 2, the inside of the injection tube 2 is provided with a push-pull rod 21, the top of the push-pull rod 21 is fixedly connected with the piston rod of the electric telescopic cylinder 3, the bottom is provided with a piston 22, a liquid suction tube 23 is arranged below the injection tube 2, the bottom end of the liquid suction tube 23 penetrates through the storage tank 4 and extends to the inner side thereof, the outer wall of the liquid suction tube 23 is provided with a first throttle valve 231, the upper part of the first throttle valve 231 is provided with a liquid conveying tube 24, the outer wall of the liquid conveying tube 24 is provided with a second throttle valve, the two ends of the liquid conveying tube 24 are respectively communicated with the preparation tank 1 and the liquid suction tube 23, just preparation jar 1 includes outer jar of body 11 and inner tank body 12, be equipped with heating chamber 14 between outer jar of body 11 and the inner tank body 12, be equipped with spiral heating coil 15 in the heating chamber 14, just the inboard of the inner tank body 12 is equipped with puddler 16, radially be equipped with multiunit paddle formula stirring leaf 161 on the outer wall of puddler 16, just outer jar of body 11 top is equipped with motor 13, motor 13's output shaft runs through outer jar of body 11's roof and 16 top fixed connection of puddler.

Further, in the above technical scheme, a collecting tank 7 is arranged behind the preparation tank 1, an exhaust pipe 71 is arranged above the collecting tank 7, two ends of the exhaust pipe 71 are respectively communicated with the preparation tank 1 and the collecting tank 7, a control valve 711 is installed on the lower portion of the exhaust pipe 71, cold water is injected into the collecting tank 7 to form a low-temperature environment, the control valve 711 is opened to enable ethanol or ether gas to enter the collecting tank 7, and in the low-temperature environment, the ethanol or ether gas can be liquefied, so that the collection of ethanol or ether is realized, and the later-stage reuse of the ethanol or ether by a user is facilitated.

Further, in the above technical solution, a pressure relief pipe 72 is installed at the top of the collection tank 7, a pressure relief valve 721 is installed on the outer wall of the pressure relief pipe 72, and the excess gas in the collection tank 7 can be discharged by opening the pressure relief valve 721, so as to prevent explosion caused by an excessive pressure in the collection tank 7.

Further, in the above technical scheme, a water heater 6 is installed on the outer wall of the preparation tank 1 through a hoop, a water outlet of the water heater 6 is connected with a water inlet of the spiral heating coil 15 through a pipeline, a water inlet of the water heater 6 is connected with a circulating water pump 8 through a pipeline, the water heater 6 is used for heating the solution, and ethanol or ether in the solution is removed.

Further, in the above technical scheme, one side of preparation jar 1, which is far away from storage tank 4, is equipped with water storage tank 5, water storage tank 5 passes through the pipeline and links to each other with circulating water pump 8's water inlet and spiral heating coil 15's delivery port respectively, realizes that hydrothermal circulation flows.

Further, in the above technical scheme, the top of the preparation tank 1 and the top of the storage tank 4 are both provided with a feed inlet 9, the feed inlet 9 is in one of a circle, a square and a rectangle, the feed inlet 9 at the top of the preparation tank 1 is convenient for a user to add water-soluble drugs (water phase) into the preparation tank 1, the feed inlet 9 at the top of the storage tank 4 is convenient for the user to add lipid-like and lipid-soluble drugs dissolved in an organic solvent into the storage tank 4, and the organic solvent is ethanol or ether.

This practical theory of operation:

referring to the attached drawings 1-4 of the specification, the liquid is heated by hot water circulating in the spiral heating coil 15, the push-pull rod 21 is controlled to move upwards by the electric telescopic cylinder 3, the first throttle valve 231 is opened, the second throttle valve is closed, the liquid in the storage tank 4 enters the injection tube 2 under the action of negative pressure, then the first throttle valve 231 is closed, the second throttle valve is opened, the push-pull rod 21 is controlled to move downwards by the electric telescopic cylinder 3, at the moment, the liquid in the injection tube 2 is injected into the preparation tank 1 at a constant speed, then the stirring rod 16 is controlled by the motor 13 to drive a plurality of groups of paddle type stirring blades 161 to rotate to stir the liquid, and after ethanol or ether in the liquid is removed, the preparation of the liposome can be completed, the operation is simple, the injection amount of the oil phase is conveniently controlled, time and labor are saved, the preparation efficiency of the liposome can be greatly improved, and the error is small, the quality of the product can be improved;

referring to the attached figures 1-4 of the specification, water in a water storage tank 5 is sent to a water heater 6 through a circulating water pump 8 to be heated, then the heated water enters a spiral heating coil 15, and hot water circularly flows in the spiral heating coil 15 through the arranged circulating water pump 8, so that the water resource is reused;

referring to the attached drawings 1-4 of the specification, cold water is injected into the collection tank 7 to form a low-temperature environment, the control valve 711 is opened to enable the ethanol or ether gas to enter the collection tank 7, and the ethanol or ether gas can be liquefied in the low-temperature environment, so that the collection of the ethanol or ether is realized, and the ethanol or ether is convenient for a user to recycle at the later stage.

While certain exemplary embodiments of the present invention have been described above by way of illustration only, it will be apparent to those of ordinary skill in the art that the described embodiments may be modified in various different ways without departing from the spirit and scope of the present invention. Accordingly, the drawings and description are illustrative in nature and should not be construed as limiting the scope of the invention.

Claims

1. A liposome prepares instrument fast, includes preparation jar (1), its characterized in that: one side of the preparation tank (1) is provided with a storage tank (4), the outer wall of the preparation tank (1) is provided with a fixing plate (31), the bottom of the fixing plate (31) is vertically provided with an electric telescopic cylinder (3), the lower part of the electric telescopic cylinder (3) is provided with an injection tube (2), the inner part of the injection tube (2) is provided with a push-pull rod (21), the top of the push-pull rod (21) is fixedly connected with the piston rod of the electric telescopic cylinder (3), the bottom of the injection tube (2) is provided with a piston (22), the lower part of the injection tube (2) is provided with a liquid pumping tube (23), the bottom end of the liquid pumping tube (23) penetrates through the storage tank (4) and extends to the inner side of the storage tank, the outer wall of the liquid pumping tube (23) is provided with a first throttle valve (231), the upper part of the first throttle valve (231) is provided with a liquid conveying tube (24), the outer wall of the liquid conveying tube (24) is provided with a second throttle valve, and the two ends of the liquid conveying tube (24) are respectively communicated with the preparation tank (1) and the liquid pumping tube (23), just preparation jar (1) is including the outer jar of body (11) and the inner tank body (12), be equipped with between the outer jar of body (11) and the inner tank body (12) and heat chamber (14), be equipped with spiral heating coil (15) in heating chamber (14), just the inboard of the inner tank body (12) is equipped with puddler (16), radially be equipped with multiunit paddle formula stirring leaf (161) on the outer wall of puddler (16), just the outer jar of body (11) top is equipped with motor (13), the output shaft of motor (13) runs through the roof and puddler (16) top fixed connection of the outer jar of body (11).

2. The apparatus for rapidly preparing liposome according to claim 1, wherein: the rear of preparation jar (1) is equipped with holding vessel (7), the top of holding vessel (7) is equipped with blast pipe (71), the both ends of blast pipe (71) are linked together with preparation jar (1) and holding vessel (7) respectively, just control valve (711) are installed to the lower part of blast pipe (71).

3. The apparatus for rapidly preparing liposome according to claim 2, wherein: the top of collection tank (7) is installed pressure release pipe (72), install relief valve (721) on the outer wall of pressure release pipe (72).

4. The apparatus for rapidly preparing liposome according to claim 1, wherein: install water heater (6) through the staple bolt on the outer wall of preparation jar (1), the delivery port of water heater (6) passes through the pipeline and links to each other with the water inlet of spiral heating coil (15), the water inlet of water heater (6) has circulating water pump (8) through the pipe connection.

5. The apparatus for rapidly preparing liposome according to claim 4, wherein: one side of the preparation tank (1) far away from the storage tank (4) is provided with a water storage tank (5), and the water storage tank (5) is respectively connected with a water inlet of the circulating water pump (8) and a water outlet of the spiral heating coil (15) through pipelines.

6. The apparatus for rapidly preparing liposome according to claim 1, wherein: the top of the preparation tank (1) and the top of the storage tank (4) are both provided with a feed inlet (9), and the shape of the feed inlet (9) is one of circular, square and rectangular.