CN214937539U

CN214937539U - Cell co-culture dish

Info

Publication number: CN214937539U
Application number: CN202120751462.2U
Authority: CN
Inventors: 罗碧辉; 刘沛颖; 王金萍; 曾小钧; 柯梦梦
Original assignee: First Affiliated Hospital of Guangzhou Medical University
Current assignee: First Affiliated Hospital of Guangzhou Medical University
Priority date: 2021-04-13
Filing date: 2021-04-13
Publication date: 2021-11-30
Anticipated expiration: 2031-04-13

Abstract

The utility model relates to a biological culture technical field discloses a cell co-culture dish, including outer culture dish and the interior culture dish of arranging in outer culture dish, be provided with the brace table that is used for supporting interior culture dish on the outer culture dish, interior culture dish hangs the dress on the brace table, has the interval between the diapire of interior culture dish and outer culture dish, and the interval forms the chamber that holds that is used for holding the cultured cell, still is equipped with the circulation passageway that supplies the culture medium circulation between interior culture dish and the outer culture dish. When cell co-culture is carried out, the inner culture dish is placed in a common culture dish to culture the myocardial cells, the inner culture dish and the myocardial cells are transferred into the outer culture dish together after the morphology and the cell activity of the myocardial cells meet the experimental requirements, and the inner culture dish plays a role of a slide climbing and avoids damaging the slide climbing; in the culture medium of outer culture dish passed through flow channel entering interior culture dish, interior culture dish and the interior cell of outer culture dish can not direct contact owing to the interval, avoid haring the fibroblast.

Description

Cell co-culture dish

Technical Field

The utility model relates to a biological culture technical field especially relates to a cell co-culture dish.

Background

In the process of making mouse cardiac fibroblasts and cardiomyocytes, the cardiac fibroblasts and cardiomyocytes need to be co-cultured, firstly, the fibroblasts need to be cultured in a culture dish, in addition, a cell slide is prepared to seed the cardiomyocytes in another culture dish, after the morphology and the cell activity of the cardiomyocytes meet the experimental requirements, the slide with the cardiomyocytes is taken out, and the slide is transferred to the culture dish with the fibroblasts for co-culture.

Mouse heart fibroblast and cardiomyocyte coculture in-process because operating problem has the risk of damaging the slide when shifting the slide, in addition, the slide is directly placed in the culture dish when shifting the slide to the kind has fibroblast's culture dish on, and slide and fibroblast direct contact can lead to the fibroblast damage in the scope that the slide contacted, influence the experimental result.

SUMMERY OF THE UTILITY MODEL

The utility model aims at: provides a cell co-culture dish to solve the problem that the slide and the fibroblasts are damaged when the slide is transferred in the cell co-culture process in the prior art.

In order to realize the above-mentioned purpose, the utility model provides a cell co-culture dish, be in including outer culture dish and arrangement interior culture dish in the outer culture dish, be provided with on the outer culture dish and be used for supporting the brace table of interior culture dish, interior culture dish is hung the dress and is in on the brace table, interior culture dish with the interval has between the diapire of outer culture dish, the interval forms the chamber that holds that is used for holding the cultured cell, interior culture dish with still be equipped with the circulation passageway that supplies the culture medium circulation between the outer culture dish.

Preferably, the inner culture dish comprises a dish body and a hanging table arranged at the edge of the dish body, and the hanging table is higher than the dish body.

Preferably, the hanging table is a hook integrally formed with the dish body, and a plurality of hooks are arranged at intervals along the circumferential direction of the dish body.

Preferably, the hook comprises a connecting section connected with the dish body, a hook section parallel to the connecting section and hooked on the supporting table, and a transition section connected between the connecting section and the hook section, wherein the lowest point of the hook section is higher than that of the dish body.

Preferably, the inner culture dish is further provided with a liquid changing gap, and the liquid changing gap forms the flow channel.

Preferably, the liquid change gap is of a crescent structure.

Preferably, the support platform is arranged on an inner wall of the outer culture dish, the support platform being an inner ring platform extending along a circumferential direction of the outer culture dish.

Preferably, the cell co-culture dish further comprises a dish cover, and the dish cover is covered on the outer culture dish.

The embodiment of the utility model provides a cell co-culture dish compares with prior art, and its beneficial effect lies in: an inner culture dish is arranged in the outer culture dish and is hung on a supporting table of the outer culture dish, when cell co-culture is carried out, the inner culture dish is firstly placed in a common culture dish to culture the myocardial cells, the inner culture dish and the myocardial cells are transferred into the outer culture dish together after the shapes and the cell activities of the myocardial cells meet the experimental requirements, the inner culture dish is hung on the supporting table, the inner culture dish plays a role of a slide, the inner culture dish is large in size and convenient to transfer, and the slide is prevented from being damaged; simultaneously, an interval is arranged between the inner culture dish and the outer culture dish, the culture medium of the outer culture dish enters the inner culture dish through the flow channel, cells in the inner culture dish and the outer culture dish cannot be in direct contact with each other, damage to fibroblasts is avoided, and the accuracy of an experimental result is guaranteed.

Drawings

FIG. 1 is a cross-sectional view showing the structure of a cell co-culture dish according to the present invention;

FIG. 2 is a schematic diagram of the structure of the inner culture dish of the cell co-culture dish of FIG. 1.

In the figure, 1, an outer culture dish; 2. an inner culture dish; 21. a dish body; 22. hooking; 221. a connecting section; 222. a transition section; 223. a hooking section; 3. a dish cover; 4. a support table; 5. and (6) replacing the liquid gap.

Detailed Description

The following detailed description of the embodiments of the present invention is provided with reference to the accompanying drawings and examples. The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention.

The utility model discloses a cell co-culture dish's preferred embodiment, as shown in FIG. 1 and FIG. 2, this cell co-culture dish includes outer culture dish 1, interior culture dish 2 and ware lid 3, and the removable arrangement of interior culture dish 2 is in

outer culture dish

1, and 3 covers of ware lid are established on outer culture dish 1, and ware lid 3 is used for sealing the culture dish, reduces external environment to cell culture's influence. The inner culture dish 2, the outer culture dish 1 and the dish cover 3 are all made of common culture dishes, and the requirements of economy, difficulty in damage, flexible specification design and the like are met.

The outer culture dish 1 is cylindrical, the top of the outer culture dish 1 is of an open structure, and the dish cover 3 covers the top of the outer culture dish 1. Outer culture dish 1 is provided with brace table 4, and brace table 4 is used for supporting interior culture dish 2, and interior culture dish 2 hangs the dress on brace table 4, and interior culture dish 2 plays the effect of climbing the piece arranging on outer culture dish 1, and it has the myocardial cell to plant on it, and the mode assembly of hanging the dress is simple, can avoid leading to the fact the damage to interior culture dish 2.

Preferably, the support platform 4 is arranged on the inner wall of the outer culture dish 1, the support platform 4 is an inner ring platform extending along the circumferential direction of the outer culture dish 1, the support surface of the inner ring platform is large, and the inner culture dish 2 can be stably supported within the range of 360 degrees in the circumferential direction. In other embodiments, the support platform 4 may be a step platform arranged at intervals along the circumference of the outer culture dish 1, and the inner culture dish 2 is hung on the step platform.

Interior culture dish 2 includes the dish body 21 and hangs the platform, and the dish body 21 is the carrier that is used for cultivateing cardiomyocyte, hangs the platform and arranges the marginal position at the dish body 21, hangs the platform and hangs the dress cooperation with a supporting bench 4, makes interior culture dish 2 hang and adorn on a supporting bench 4. The hanging table is higher than the dish body 21, namely the hanging table protrudes out of the surface of the dish body 21, when the culture dish 2 is transferred, an operator can transfer the culture dish 2 through the hanging table, and the damage to the myocardial cells on the dish body 21 is avoided.

Have the interval between the diapire of the dish body 21 and outer culture dish 1, this interval forms the chamber that holds that is used for holding the cultured cell, is equipped with the circulation channel who supplies the culture medium circulation between the dish body 21 of interior culture dish 2 and outer culture dish 1, makes culture medium by holding chamber entering interior culture dish 2, covers the cardiomyocyte on interior culture dish 2. The dish body 21 is lower than the hanging platform, and after the hanging platform is hung on the culture dish, the dish body 21 is closer to the bottom of the outer culture dish 1, so that the culture medium can conveniently enter the inner culture dish 2.

Preferably, the hanging table is a hook 22, the hook 22 and the dish body 21 are of an integrally formed structure, the four hooks 22 are arranged at intervals along the circumferential direction of the dish body 21, the four hooks 22 are uniformly distributed in a cross shape, and the stability of the dish body 21 can be improved by the four hooks 22. In other embodiments, the hooks 22 can be three, five, six, etc. The couple 22 extends to the outside of dish body 21 and arranges, forms the arc interval between two adjacent couples 22 and between the interior culture dish 2, and the culture medium can get into the dish body 21 in the arc interval, fully covers the cardiomyocyte on the interior culture dish 2, reaches the effect of supporting altogether.

In other embodiments, the hanging table may be an L-shaped flange disposed on the vessel body 21, and the vessel body 21 is hung on the supporting table 4 through the L-shaped flange.

The hook 22 comprises a connecting section 221 connected with the dish body 21, a hooking section 223 parallel to the connecting section 221 and hooked on the support platform 4, and a transition section 222 connected between the connecting section 221 and the hooking section 223, wherein the connecting section 221, the hooking section 223 and the transition section 222 are integrally formed and form a U-shaped structure. The lowest point of the hooking section 223 is higher than the dish body 21, when the inner culture dish 2 is hooked on the supporting table 4, the dish body 21 is lower than the hooking section 223, the interval between the bottom walls of the dish body 21 and the outer culture dish 1 is reduced, and the culture medium can conveniently enter the dish body 21.

Preferably, still be equipped with on the interior culture dish 2 and trade liquid breach 5, trade liquid breach 5 and arrange between two adjacent couples 22, trade liquid breach 5 is crescent structure, trades liquid breach 5 and forms the circulation passageway that supplies the culture medium circulation simultaneously. In addition, the liquid changing gap 5 is convenient for operators to directly absorb the co-culture medium in the culture dish, the inner culture dish 2 does not need to be taken out, the liquid is convenient to change, and the risk of cell pollution is reduced.

To sum up, the embodiment of the utility model provides a cell co-culture dish, arrange the interior culture dish in its outer culture dish, and interior culture dish is hung on the brace table of outer culture dish, when carrying out cell co-culture, place interior culture dish in ordinary culture dish earlier and cultivate cardiomyocyte, transfer interior culture dish and cardiomyocyte together in outer culture dish after the form of cardiomyocyte, cell activity reach the experimental requirement, interior culture dish is hung on the brace table, interior culture dish plays the effect of slide, the bulky transfer convenience of interior culture dish avoids haring the slide; simultaneously, an interval is arranged between the inner culture dish and the outer culture dish, the culture medium of the outer culture dish enters the inner culture dish through the flow channel, cells in the inner culture dish and the outer culture dish cannot be in direct contact with each other, damage to fibroblasts is avoided, and the accuracy of an experimental result is guaranteed.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and replacements can be made without departing from the technical principle of the present invention, and these modifications and replacements should also be regarded as the protection scope of the present invention.

Claims

1. The utility model provides a cell co-culture dish, its characterized in that includes outer culture dish and arranges interior culture dish in the outer culture dish, be provided with on the outer culture dish and be used for supporting the brace table of interior culture dish, interior culture dish hangs the dress and is in on the brace table, interior culture dish with the interval has between the diapire of outer culture dish, the interval forms the chamber that holds that is used for holding the cultured cell, interior culture dish with still be equipped with the circulation passageway that supplies the culture medium circulation between the outer culture dish.

2. A cell co-culture dish according to claim 1, wherein the inner culture dish comprises a dish body and a hanging platform arranged at the rim of the dish body, the hanging platform being arranged higher than the dish body.

3. A cell co-culture dish according to claim 2, wherein the hanging platform is a hook integrally formed with the dish body, and a plurality of hooks are arranged at intervals along the circumferential direction of the dish body.

4. A cell co-culture dish according to claim 3, wherein the hook comprises a connection section connected to the dish body, a hooking section parallel to the connection section for hooking to the support table, and a transition section connected between the connection section and the hooking section, wherein the hooking section has a lowest point higher than the dish body.

5. A cell co-culture dish according to any of claims 1-4, wherein the inner culture dish is further provided with a fluid exchange gap, the fluid exchange gap forming the flow channel.

6. A cell co-culture dish according to claim 5, wherein the fluid change gap is a crescent-shaped structure.

7. A cell co-culture dish according to any of claims 1-4, wherein the support platform is arranged on an inner wall of the outer culture dish, the support platform being an inner ring platform extending in a circumferential direction of the outer culture dish.

8. A cell co-culture dish according to any of claims 1 to 4, further comprising a dish lid, the dish lid housing the outer culture dish.