CN214881595U

CN214881595U - Bioreactor with efficient ventilation device

Info

Publication number: CN214881595U
Application number: CN202121517648.8U
Authority: CN
Inventors: 姜海
Original assignee: Biouteck Biological Technology Service Beijing Co ltd
Current assignee: Biouteck Biological Technology Service Beijing Co ltd
Priority date: 2021-07-05
Filing date: 2021-07-05
Publication date: 2021-11-26
Anticipated expiration: 2031-07-05

Abstract

The utility model discloses a bioreactor with high-efficient breather belongs to bioreactor technical field, including bioreactor body, breather, fixing device and gas filtration device, breather is located bioreactor body, breather passes through fixing device and is connected with the bioreactor, the vertical setting of breather is at bioreactor body, gas filtration device is located the top of bioreactor body and is linked together with breather. The utility model discloses can expand and satisfy the required oxygen concentration of cell, ensure the effective degradation to the pollutant.

Description

Bioreactor with efficient ventilation device

Technical Field

The utility model relates to a bioreactor technical field especially relates to a bioreactor with high-efficient breather.

Background

The bioreactor refers to a reaction system in which a liquid phase or a solid phase is inoculated with naturally occurring microorganisms or microorganisms having a specific degradation ability. The two most studied reactors today are the "elevator type reactor" and the "soil mud reactor". The elevator type reactor provides proper nutrition, carbon source and oxygen through the flow of water phase, thereby achieving the purpose of degrading pollutants in soil. Compared with a solid phase system, the bioreactor can effectively degrade pollutants in a shorter time. The bioreactor technology has been applied to bioremediation of organically contaminated soil.

At present, in a suspension system bioreactor in the market, a hose microbubble permeation technology is adopted in a ventilation link, the ventilation quantity is insufficient, the expansion is difficult to meet the oxygen concentration required by cells, or large bubbles are adopted for ventilation, so that the cell rupture loss is caused.

SUMMERY OF THE UTILITY MODEL

The embodiment of the utility model provides a bioreactor with high-efficient breather to solve the problem among the prior art.

The embodiment of the utility model provides an adopt following technical scheme: a bioreactor with a high-efficiency ventilating device comprises a bioreactor body, a ventilating device, a fixing device and a gas filtering device, wherein the ventilating device is positioned in the bioreactor body and is connected with the bioreactor through the fixing device, the ventilating device is vertically arranged in the bioreactor body, and the gas filtering device is positioned at the top of the bioreactor body and is communicated with the ventilating device.

Furthermore, the ventilation device comprises a ventilation pipe, an upper connecting pipe head and a lower connecting pipe head, wherein external threads are arranged on the upper connecting pipe head and the lower connecting pipe head, internal threads are arranged at two ends of the ventilation pipe, and the upper connecting pipe head and the lower connecting pipe head are in threaded connection with two ends of the ventilation pipe.

Further, the outer surface of the breather pipe is of a porous structure.

Further, the shape of the vent pipe is cylindrical.

Further, an air inlet pipe is arranged on the upper connecting pipe head and is in threaded connection with the upper connecting pipe.

Further, fixing device includes mount and two fixing bolt, the mount passes through two fixing bolt and connects on the lateral wall of breather pipe, the mount is connected with bioreactor body's interior roof detachable.

Further, gas filtering device includes rose box and filter screen, the rose box is located bioreactor's top and is linked together with the top of intake pipe, filter screen detachable connects on the rose box.

Further, the bottom of the lower connecting pipe head is connected with an exhaust filter, and an exhaust pipe extending out of the bioreactor body is arranged on the exhaust filter.

The embodiment of the utility model provides an above-mentioned at least one technical scheme who adopts can reach following beneficial effect: the ventilation cavity adopts a porous structure (the aperture is about 10 microns), the inside and the outside of the cavity are in fluid communication, the micropores are fully isolated for suspended cells (such as CHO cells) to ensure that the cells can not enter the ventilation tube, and media (culture media) inside and outside the cavity can be fully exchanged. Because cells do not exist in the cavity, large bubbles are adopted for ventilation, and the damage of the bubbles to the cells is not needed to be avoided, thereby realizing a high dissolved oxygen state.

Drawings

The accompanying drawings, which are described herein, serve to provide a further understanding of the invention and constitute a part of this specification, and the exemplary embodiments and descriptions thereof are provided for explaining the invention without unduly limiting it. In the drawings:

fig. 1 is a schematic perspective view of the present invention;

fig. 2 is a cross-sectional view of the present invention;

fig. 3 is a perspective sectional view of the present invention;

FIG. 4 is a perspective view of the vent apparatus of the present invention;

reference numerals:

bioreactor body 1, breather 2, breather pipe 21, go up connector 22, lower connector 23, fixing device 3, mount 31, fixing bolt 32, gas filter equipment 4, rose box 41, filter screen 42, intake pipe 5, exhaust filter 6, blast pipe 61.

Detailed Description

To make the purpose, technical solution and advantages of the present invention clearer, the following will combine the embodiments of the present invention and the corresponding drawings to clearly and completely describe the technical solution of the present invention. It is to be understood that the embodiments described are only some embodiments of the invention, and not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1 to 4, the embodiment of the present invention provides a bioreactor with a high efficiency aeration device, including a bioreactor body 1, an aeration device 2, a fixing device 3 and a gas filtering device 4, wherein the aeration device 2 is located in the bioreactor body 1, the aeration device 2 is connected with the bioreactor through the fixing device 3, the aeration device 2 is vertically disposed in the bioreactor body 1, and the gas filtering device 4 is located at the top of the bioreactor body 1 and is communicated with the aeration device 2. When the device is vertically installed, the height of the upper end of the vent pipe 21 needs to exceed the liquid level of the working liquid of the reactor.

Specifically, breather 2 includes breather pipe 21, goes up connector 22 and connector 23 down, go up connector 22 and all be equipped with the external screw thread down on the connector 23, the both ends of breather pipe 21 all are equipped with the internal thread, go up connector 22 and connector 23 down and breather pipe 21's both ends threaded connection. The detachment of the vent tube 21 from the upper connector 22 and the lower connector 23 can be facilitated.

Specifically, the outer surface of the air pipe 21 is a porous structure. The ventilation cavity 21 adopts a porous structure (the pore diameter is about 10 microns), the inside and the outside of the cavity are in fluid communication, the micropores are fully isolated for suspended cells (such as CHO cells) to ensure that the cells can not enter the ventilation tube, and media (culture media) inside and outside the cavity can be fully exchanged. Because cells do not exist in the cavity, large bubbles are adopted for ventilation, and the damage of the bubbles to the cells is not needed to be avoided, thereby realizing a high dissolved oxygen state.

The vent pipe 21 is made of porous material sintered by pharmaceutical grade 316L stainless steel powder; the vent tube 21 may also be made of other materials, such as pharmaceutical grade ceramics, pharmaceutical grade nickel powder sintered, pharmaceutical grade microporous organic membranes.

Specifically, the vent pipe 21 has a cylindrical shape. The shape of the vent tube 21 can be made into any shape which is convenient for operation and does not affect the cell breeding space.

Specifically, the upper connector head 22 is provided with an air inlet pipe 5, and the air inlet pipe 5 is in threaded connection with the upper connecting pipe. The air inlet pipe 5 conveys oxygen into the air vent pipe 21 for subsequent cell amplification, and the air vent pipe 21 can be detached from the air inlet pipe 5 by adopting threaded connection, so that the air vent pipe 21 is convenient to replace.

Specifically, the fixing device 3 includes a fixing frame 31 and two fixing bolts 32, the fixing frame 31 is connected to the side wall of the air pipe 21 through the two fixing bolts 32, and the fixing frame 31 is detachably connected to the inner top wall of the bioreactor body 1. Can fix breather pipe 21 on mount 31 through two fixing bolt 32, can be used for the fixed to breather pipe 21, mount 31 can be dismantled to connect and can be dismantled breather pipe 21, change breather pipe 21.

Specifically, the gas filtering device 4 comprises a filtering box 41 and a filtering net 42, the filtering box 41 is positioned at the top of the bioreactor and communicated with the top of the gas inlet pipe 5, and the filtering net 42 is detachably connected to the filtering box 41. Oxygen gets into in the rose box 41, and the filter screen 42 in the rose box 41 can filter the impurity in the oxygen, prevents that there is impurity to advance in the bioreactor body 1, causes the porous structure on the breather pipe 21 to block up, and filter screen 42 can be dismantled and to change it.

Specifically, the bottom of the lower connector 23 is connected to an exhaust filter 6, and the exhaust filter 6 is provided with an exhaust pipe 61 extending to the outside of the bioreactor body 1. The exhaust gas filter 6 filters the exhaust gas, and delivers the filtered gas to the outside of the bioreactor body 1 through the exhaust pipe 61.

The above description is only an example of the present invention, and is not intended to limit the present invention. Various modifications and changes may occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the scope of the claims of the present invention.

Claims

1. The bioreactor with the efficient ventilation device is characterized by comprising a bioreactor body (1), the ventilation device (2), a fixing device (3) and a gas filtering device (4), wherein the ventilation device (2) is located in the bioreactor body (1), the ventilation device (2) is connected with the bioreactor through the fixing device (3), the ventilation device (2) is vertically arranged in the bioreactor body (1), and the gas filtering device (4) is located at the top of the bioreactor body (1) and communicated with the ventilation device (2).

2. A bioreactor having a high efficiency aeration device according to claim 1 wherein: the ventilation device (2) comprises a ventilation pipe (21), an upper connecting pipe head (22) and a lower connecting pipe head (23), wherein external threads are arranged on the upper connecting pipe head (22) and the lower connecting pipe head (23), internal threads are arranged at two ends of the ventilation pipe (21), and the upper connecting pipe head (22) and the lower connecting pipe head (23) are in threaded connection with two ends of the ventilation pipe (21).

3. A bioreactor having a high efficiency aeration device according to claim 2 wherein: the outer surface of the vent pipe (21) is of a porous structure.

4. A bioreactor having a high efficiency aeration device according to claim 2 wherein: the vent pipe (21) is cylindrical in shape.

5. A bioreactor having a high efficiency aeration device according to claim 2 wherein: the upper connecting pipe head (22) is provided with an air inlet pipe (5), and the air inlet pipe (5) is in threaded connection with the upper connecting pipe.

6. A bioreactor having a high efficiency aeration device according to claim 2 wherein: fixing device (3) are including mount (31) and two fixing bolt (32), mount (31) are connected on the lateral wall of breather pipe (21) through two fixing bolt (32), mount (31) are connected with the interior roof detachable of bioreactor body (1).

7. A bioreactor having a high efficiency aeration device according to claim 5 wherein: the gas filtering device (4) comprises a filtering box (41) and a filtering net (42), wherein the filtering box (41) is positioned at the top of the bioreactor and communicated with the top of the gas inlet pipe (5), and the filtering net (42) is detachably connected to the filtering box (41).

8. A bioreactor having a high efficiency aeration device according to claim 2 wherein: the bottom of lower connector head (23) is connected with exhaust filter (6), be equipped with on exhaust filter (6) and extend to bioreactor body (1) outer blast pipe (61).