CN214781850U

CN214781850U - Sampling device for fluorescent quantitative PCR instrument

Info

Publication number: CN214781850U
Application number: CN202120618286.5U
Authority: CN
Inventors: 刘文娜; 魏仙仙; 王琪
Original assignee: Qingdao Wanming Saibo Pharmaceutical Co ltd
Current assignee: Qingdao Wanming Saibo Pharmaceutical Co ltd
Priority date: 2021-03-26
Filing date: 2021-03-26
Publication date: 2021-11-19
Anticipated expiration: 2031-03-26

Abstract

The utility model discloses a sampling device for a fluorescence quantitative PCR instrument, which comprises a sleeve, movable rods are arranged on both sides of the bottom end of a push-pull block, a positioning disc is fixedly connected with the bottom end of the movable rods, a movable block is movably sleeved on the outer side of the sleeve, both sides of the top end of the movable block are provided with fixed holes, scales are evenly arranged on the outer side of the sleeve, a fixed block is fixedly arranged on one side of the top end of the movable block, a positioning screw rod penetrates through the top end of the side surface of the fixed block through the movement of a thread, a needle head is arranged at the bottom end of the sleeve, the movable block slides to a corresponding scale position by sliding the movable block on the outer side of the sleeve, and then the position of the movable block is fixed by screwing the positioning screw rod, so that the movable rods can only move upwards to the positioning disc to abut against the bottom end of the movable block, thereby controlling the distance that the push-pull block drives a piston rod to pull upwards, and controlling the sample extraction amount of the device according to the scales, can effectively prevent the sample from being extracted and wasted.

Description

Sampling device for fluorescent quantitative PCR instrument

Technical Field

The utility model relates to the technical field of medical equipment, in particular to a sampling device for a fluorescence quantitative PCR instrument.

Background

A real-time fluorescent quantitative PCR instrument is composed of a fluorescent quantitative system and a computer, and is used for monitoring the fluorescence in the cyclic process, the computer connected with a real-time device collects fluorescence data, the data are displayed in a form of a graph through developed real-time analysis software, and original data are drawn into a graph of fluorescence intensity relative to the cyclic number. The analysis can be started after the original data is collected, the software of the real-time equipment can enable the collected data to be subjected to normalization processing to make up for the difference of background fluorescence, a threshold value level can be set after normalization, namely the level of the fluorescence data is analyzed, and the PCR instrument needs to sample samples before sample detection.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a sampling device for fluorescence quantitative PCR appearance to solve and to propose in the above-mentioned background art because traditional sample is mostly extracted the sample through ordinary needle tubing, the volume of this kind of method uncontrollable extraction easily causes the problem of the waste of sample.

In order to achieve the above object, the utility model provides a following technical scheme: a sampling device for a fluorescence quantitative PCR instrument comprises a sleeve, wherein a piston rod is slidably connected to the inner side of the sleeve, a push-pull block is fixedly connected to the top end of the piston rod, a quantitative assembly is mounted on the outer side of the sleeve, and the quantitative assembly comprises a movable rod, a positioning disc, a movable block, a fixing hole, scales, a fixing block, a positioning screw rod and a needle head;

the utility model discloses a syringe needle, including push-and-pull piece, movable rod bottom fixedly connected with positioning disk, the movable block has been cup jointed in the activity of sleeve outside, the fixed orifices has all been seted up to movable block top both sides, the scale has evenly been seted up in the sleeve outside, movable block top one side fixed mounting has the fixed block, fixed block side top has run through positioning screw through the screw thread activity, the syringe needle is installed to the sleeve bottom.

Preferably, the bottom ends of the two movable rods respectively and movably penetrate through the fixed holes on the two sides of the top end of the movable block.

Preferably, the inner diameter of the fixed hole is larger than the diameter of the movable rod, and the diameter of the positioning plate is larger than the diameter of the fixed hole.

Preferably, the locating hole has all been seted up to push away and draw piece bottom both sides, two movable rod outside top is sliding connection in two locating hole inboards respectively, the fixed stop collar that has cup jointed in movable rod outside top, movable rod top fixedly connected with thread head, the thread head outside has cup jointed the internal thread cap through the screw thread.

Preferably, the top end of the limiting ring is tightly attached to the bottom end of the push-pull block, the outer diameter of the limiting ring is larger than the inner diameter of the positioning hole, the diameter of the thread head is smaller than the inner diameter of the positioning hole, and the outer diameter of the internal thread cap is larger than the inner diameter of the positioning hole.

Compared with the prior art, the beneficial effects of the utility model are that:

1. through sliding the movable block outside the sleeve, making the movable block slide to corresponding scale position, then make the movable block rigidity through screwing up positioning screw to make the movable rod can only upwards move about to the positioning disk and support the movable block bottom, thereby the control pushes away the distance that draws that the piece drove the piston rod upwards, thereby can controlling means's sample extraction volume according to the scale, can effectually prevent that the extraction of sample is extravagant.

2. Through the internal thread cap of rotatory back-out thread head outside to can make and carry out the split between movable rod and the push-and-pull piece, thereby be convenient for whole device to carry out the split clearance or change.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of the quantitative assembly of the present invention;

fig. 3 is a schematic view of the mounting structure of the middle screw head of the present invention.

In the figure: 1. a sleeve; 2. a piston rod; 3. a push-pull block; 4. a dosing assembly; 401. a movable rod; 402. positioning a plate; 403. a movable block; 404. a fixing hole; 405. calibration; 406. a fixed block; 407. positioning a screw rod; 408. a needle head; 5. positioning holes; 6. a limiting ring; 7. a screw head; 8. an internally threaded cap.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-3, the present invention provides a technical solution: a sampling device for a fluorescence quantitative PCR instrument comprises a sleeve 1, wherein the inner side of the sleeve 1 is slidably connected with a piston rod 2, the top end of the piston rod 2 is fixedly connected with a push-pull block 3, the outer side of the sleeve 1 is provided with a quantitative assembly 4, and the quantitative assembly 4 comprises a movable rod 401, a positioning disc 402, a movable block 403, a fixed hole 404, scales 405, a fixed block 406, a positioning screw 407 and a needle head 408;

all install movable rod 401 in 3 bottom both sides of push-and-pull piece, movable rod 401 bottom fixedly connected with positioning disk 402, movable block 403 has been cup jointed in the activity of the 1 outside of sleeve, and fixed orifices 404 has all been seted up to movable block 403 top both sides, and the scale 405 has evenly been seted up in the sleeve 1 outside, and fixed block 403 top one side fixed mounting has fixed block 406, and fixed block 406 side top has run through positioning screw 407 through the screw activity, and syringe needle 408 is installed to sleeve 1 bottom.

The bottom ends of the two movable rods 401 respectively and movably penetrate through the fixed holes 404 on the two sides of the top end of the movable block 403, so that the movable block 403 can slide outside the sleeve 1 relative to the movable rods 401.

The inner diameter of the fixed hole 404 is larger than the diameter of the movable rod 401, the diameter of the positioning plate 402 is larger than the diameter of the fixed hole 404, so that the positioning plate 402 cannot penetrate through the fixed hole 404, and the push-pull block 3 drives the movable rod 401 to move upwards until the positioning plate 402 abuts against the bottom end of the movable block 403.

Locating hole 5 has all been seted up to 3 bottom both sides of push-and-pull piece, and two movable rod 401 outside tops are sliding connection respectively in 5 inboards of two locating holes, and the fixed cover in the top of the 401 outside of movable rod has connect spacing ring 6, and the 401 top fixedly connected with thread head 7 of movable rod, the thread head 7 outside has cup jointed internal thread cap 8 through the screw thread, and the installation between the movable rod 401 of being convenient for and the push-and-pull piece 3 is dismantled to the split of the whole device of being convenient for.

The top end of the limiting ring 6 is tightly attached to the bottom end of the push-pull block 3, the outer diameter of the limiting ring 6 is larger than the inner diameter of the positioning hole 5, the diameter of the thread head 7 is smaller than the inner diameter of the positioning hole 5, and the outer diameter of the internal thread cap 8 is larger than the inner diameter of the positioning hole 5, so that the movable rod 401 and the push-pull block 3 can be conveniently installed and positioned.

The working principle is as follows: when sampling a sample of the PCR instrument, sliding the movable block 403 on the outer side of the sleeve 1 in advance to enable the movable block 403 to slide to a corresponding scale 405 outside the sleeve 1, namely determining the size of the sample amount to be extracted, when the movable block 403 slides to a corresponding scale 405 position, then screwing down the positioning screw 407 on the fixed block 406 to enable one end of the positioning screw 407 to firmly abut against the outer side of the sleeve 1, so that the movable block 403 cannot slide on the outer side of the sleeve 1, inserting the needle 408 into the sample, then driving the piston rod 2 to be pulled outwards through the push-pull block 3, thereby sucking the sample through the piston at the end part of the piston rod 2, driving the movable rod 401 to move together when the push-pull block 3 moves, enabling the movable rod 401 to penetrate through the movable block 403 through the fixed hole 404 until the movable rod 401 moves to enable the positioning disc 402 at the end part of the movable rod to abut against the movable block 403, thereby being unable to pull the push-pull block 3, thereby completing the sample extracted by the set scale 405, thereby be convenient for extract a quantitative sample according to the demand, can not cause the waste of sample, the internal thread cap 8 on the rotatable push-and-pull piece 3 in later stage, make internal thread cap 8 screw out from the thread head 7, thereby can follow locating hole 5 roll-off on the push-and-pull piece 3 with thread head 7 and movable rod 401 tip, thereby accomplish the split between movable rod 401 and the push-and-pull piece 3, next can carry out movable block 403, the dismantlement between movable rod 401 and the sleeve 1, thereby be convenient for the split work of whole device, so that the cleaning and disinfection or the change of each spare part.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A sampling device for a fluorescence quantitative PCR instrument, comprising a sleeve (1), characterized in that: a piston rod (2) is connected to the inner side of the sleeve (1) in a sliding manner, a push-pull block (3) is fixedly connected to the top end of the piston rod (2), a quantitative component (4) is installed on the outer side of the sleeve (1), and the quantitative component (4) comprises a movable rod (401), a positioning disc (402), a movable block (403), a fixed hole (404), scales (405), a fixed block (406), a positioning screw (407) and a needle head (408);

push away and draw piece (3) bottom both sides and all install movable rod (401), movable rod (401) bottom fixedly connected with positioning disk (402), movable block (403) have been cup jointed in the activity of sleeve (1) outside, fixed orifices (404) have all been seted up to movable block (403) top both sides, scale (405) have evenly been seted up in the sleeve (1) outside, fixed mounting has fixed block (406) on one side of movable block (403) top, fixed block (406) side top has been run through positioning screw (407) through the screw thread activity, syringe needle (408) are installed to sleeve (1) bottom.

2. The sampling device of claim 1, wherein: the bottom ends of the two movable rods (401) respectively and movably penetrate through the fixed holes (404) on the two sides of the top end of the movable block (403).

3. The sampling device of claim 1, wherein: the inner diameter of the fixed hole (404) is larger than the diameter of the movable rod (401), and the diameter of the positioning disc (402) is larger than the diameter of the fixed hole (404).

4. The sampling device of claim 1, wherein: locating hole (5), two have all been seted up to push away and draw piece (3) bottom both sides movable rod (401) outside top difference sliding connection is inboard in two locating holes (5), the fixed cover in movable rod (401) outside top has connect spacing ring (6), movable rod (401) top fixedly connected with thread head (7), thread head (7) outside has cup jointed internal thread cap (8) through the screw thread.

5. The sampling device of claim 4, wherein: the top end of the limiting ring (6) is tightly attached to the bottom end of the push-pull block (3), the outer diameter of the limiting ring (6) is larger than the inner diameter of the positioning hole (5), the diameter of the thread head (7) is smaller than the inner diameter of the positioning hole (5), and the outer diameter of the internal thread cap (8) is larger than the inner diameter of the positioning hole (5).