CN214716682U - Purification device for purifying glycoprotein - Google Patents

Abstract

The application relates to a purification device for purifying glycoprotein, it includes the material pipe, the cavity has been seted up to the material pipe, the material pipe in the cavity intussuseption is filled with anion exchange resin, the material pipe is kept away from the fluid-discharge tube is worn to be equipped with by the one end of the accent of cavity, the cavity is close to the lateral wall of fluid-discharge tube is provided with the filter membrane. The present application has the effect of reducing the cost of purifying glycoproteins.

Description

Purification device for purifying glycoprotein

Technical Field

The application relates to the technical field of purification devices, in particular to a purification device for purifying glycoprotein.

Background

When processing glycoprotein, manufacturers need to purify the unpurified primary product, and the primary product is purified to produce a purified product. At present, the purification system and the purification device for protein in the bio-pharmaceutical laboratory have complex structures and high price, and are not beneficial to the popularization and the application of the protein purification device.

The invention discloses a multi-channel protein purifier in Chinese patent 'multi-channel white purifier' with publication number CN110790813A, which comprises a plurality of channels, wherein each channel forms an independent liquid path, and each channel sequentially comprises a solvent/sample selection valve, an infusion pump, a bubble/liquid level sensor, a chromatographic column and a concentration detector according to the flow direction of the liquid path, wherein the multi-channel protein purifier is also provided with a collection module and a control module which are shared by the plurality of channels, and the control module is connected with and controls the work of the solvent/sample selection valve, the infusion pump, the bubble/liquid level sensor and the concentration detector.

In view of the above-mentioned related art, the inventors considered that since a purification apparatus for actually purifying a primary product has a complicated structure and the purchase price of the purification apparatus is high, it is difficult for a manufacturer who purifies a glycoprotein on a small scale to bear the price of the purification apparatus, and there is a drawback that the cost for purifying the glycoprotein is high.

SUMMERY OF THE UTILITY MODEL

In order to reduce the cost of purifying glycoproteins, the present application provides a purification apparatus for purifying glycoproteins.

The technical scheme is that the purification device for purifying glycoprotein provided by the application adopts the following technical scheme:

the purification device for purifying the glycoprotein comprises a material pipe, wherein a cavity is formed in the material pipe, anion exchange resin is filled in the cavity of the material pipe, a liquid discharge pipe penetrates through one end, far away from a cavity opening of the cavity, of the material pipe, and a filter membrane is arranged on the side wall, close to the liquid discharge pipe, of the cavity.

Through adopting above-mentioned technical scheme, the initial product includes impurity and the purification thing of treating the purification, and during processing the initial product, will follow the accent department of material pipe with the initial product and pour into the material pipe for the initial product contacts with anion exchange resin, and impurity in the initial product adsorbs to anion exchange resin on, and the purification thing is not adsorbed by anion exchange resin, and the purification thing flows from the fluid-discharge tube, and anion exchange resin is by the filter membrane separation, does not flow from the fluid-discharge tube. Because the purification device has simple structure and easy realization, the cost of purifying glycoprotein is reduced, and the operation is simple.

Optionally, an end cover is arranged at one end of the material pipe, which is far away from the filter membrane, and a material conveying pipe communicated with the cavity is arranged on the end cover in a penetrating manner.

By adopting the technical scheme, the material pipe is vertical when purified, and the end cover is arranged at the cavity opening of the material pipe, so that the probability that dust in the operation environment enters the material pipe to pollute the primary product and the anion exchange resin is reduced. On the other hand, when a manufacturer needs to inject materials into the cavity of the material pipe, the initial products are injected into the cavity through the material conveying pipe, and the probability that dust in the operation environment pollutes the initial products and the anion exchange resin due to the fact that the manufacturer opens the end cover to inject the materials is reduced.

Optionally, the material pipe sleeve is provided with a heat insulation pipe, an inner wall of the heat insulation pipe is provided with a heat insulation layer, and the heat insulation layer abuts against the outer wall of the material pipe.

Through adopting above-mentioned technical scheme, behind the initial product injection material pipe, the heat preservation slows down the temperature variation of initial product, reduces to separate out the probability that is difficult to filter because of the glycoprotein in the temperature variation makes mixed liquid, and then improves purification device's filtration efficiency.

Optionally, the heat preservation pipe is provided with jacks penetrating through two ends, the end cover is inserted into the jacks in an interference manner, and one end, facing the filter membrane, of the end cover is provided with a first guide surface inclined towards the filter membrane.

Through adopting above-mentioned technical scheme, during the assembly purification device, the end cover is pegged graft in the jack, and first spigot joint in the inner wall of jack earlier, and then first spigot joint slides along the inner wall of jack towards the one end of fluid-discharge tube, presses the end cover hard and makes the end cover interference peg graft in the jack, and the lateral wall of end cover supports tightly in the inner wall of jack this moment, reduces the probability that the end cover deviates from the material pipe.

Optionally, the filter membrane periphery is fixed with the membrane ring, the feed pipe is seted up and is supplied the annular of membrane ring joint.

Through adopting above-mentioned technical scheme, the secondary filtration is carried out to the primary product to the filter membrane, reduces impurity from the probability of fluid-discharge tube discharge. On the other hand, because the membrane ring joint has reduced the probability that the filter membrane takes place the offset in the annular, improves the reliability of filter membrane secondary filtration.

Optionally, the membrane ring is made of rubber.

Through adopting above-mentioned technical scheme, when the installation membrane ring, the extrusion membrane ring makes the membrane ring can follow the accent department of material pipe and impresses the cavity, and the membrane ring receives the extrusion and contracts this moment, and when the membrane ring pressed to annular department, the membrane ring reset and the joint in the annular, the outer wall of membrane ring supports tightly in the cell wall of annular for the membrane ring is difficult to deviate from in following the annular, and then makes the difficult position shift that takes place of filter membrane.

Optionally, the filter membrane comprises a plurality of pores, the pore size of the pores is smaller than the outer diameter of the anion exchange resin and the pore size of the pores is larger than the outer diameter of the purified product.

By adopting the technical scheme, after the initial product is filtered by the anion exchange resin, the purified product is discharged from the liquid discharge pipe through the filter membrane, the anion exchange resin adsorbed with impurities is blocked by the filter membrane, the probability that the anion exchange resin is discharged from the liquid discharge pipe along with the purified product is reduced, and the purity of the purified product is improved.

Optionally, the drain pipe is provided with a protruding column in a penetrating manner, one end of the protruding column is provided with a through hole, a pin shaft is rotatably inserted into the through hole, and the pin shaft is provided with a drain hole coaxial with the drain pipe.

By adopting the technical scheme, the pin shaft is rotated, and when the liquid discharge hole is communicated with the liquid discharge pipe, purified materials sequentially flow through the liquid discharge pipe and the liquid discharge hole, so that the purpose of discharging materials is achieved; when the liquid discharge hole is not communicated with the liquid discharge pipe, the purified substances are blocked by the pin shaft and do not flow out of the liquid discharge pipe, so that the aim of stopping discharging is fulfilled.

Optionally, the axial cross section of the pin shaft is isosceles trapezoid, a limiting column is fixed at one end of the pin shaft, and the limiting column is abutted to the protruding column.

By adopting the technical scheme, when the pin shaft has the tendency of moving towards the direction of the limiting column, the convex column has a limiting effect on the pin shaft, the end with the larger radius of the pin shaft is blocked by the end with the smaller aperture of the through hole, and the pin shaft cannot move towards the direction of the limiting column; when the pin shaft has the trend of moving in the direction away from the limiting column, one end of the limiting column close to the convex column is abutted against the convex column, so that the pin shaft cannot move in the direction away from the limiting column, and the pin shaft is axially fixed on the convex column.

Optionally, one end of the liquid discharge pipe, which is far away from the material pipe, is provided with a second guide surface.

Through adopting above-mentioned technical scheme, rotate the round pin axle for sell epaxial outage and not communicate in the fluid-discharge tube, remaining raffinate drips along the second spigot surface on the pipe wall of fluid-discharge tube inboard, reduces and stops to arrange the material after, the fluid-discharge tube still lasts the probability of dropping, reduces the extravagant condition of purifing thing. On the other hand, because the pollutant is easily multiplied when the one end raffinate that the material pipe was kept away from to the fluid-discharge tube is not discharged, the raffinate is followed second spigot surface discharge fluid-discharge tube, reduces the probability that the mouth of pipe pollutes.

In summary, the present application includes at least one of the following beneficial technical effects:

1. the material pipe is injected into to the initial product, and anion exchange resin adsorbs the impurity in the initial product, and the purification thing is not adsorbed by anion exchange resin and flows out from the fluid-discharge tube through the filter membrane, and anion exchange resin is then obstructed by the filter membrane, and purification device's simple structure has reduced the cost of purification glycoprotein.

2. The material pipe overcoat is equipped with the insulating tube for in the purification process, the temperature variation of initial product is little, reduces the probability that glycoprotein is appeared from mixed liquid, has improved purification device's purification efficiency.

3. The end cover is arranged at the end, far away from the liquid discharge pipe, of the material pipe, so that the probability that dust enters the material pipe and further pollutes anion exchange resin is reduced, and the frequency of replacing the anion exchange resin by a manufacturer is reduced.

Drawings

FIG. 1 is a cross-sectional view of a purification apparatus.

Fig. 2 is an enlarged view of a portion a in fig. 1.

Fig. 3 is an exploded view of the material pipe and the insulating pipe.

Description of reference numerals: 1. a chamber; 2. a material pipe; 3. a heat preservation pipe; 4. an end cap; 5. a liquid discharge pipe; 6. filtering the membrane; 7. an anion exchange resin; 8. a liquid inlet pipe; 9. a heat-insulating layer; 10. a jack; 11. a first mounting hole; 12. a pin shaft; 13. a convex column; 14. a through hole; 15. a drain hole; 16. a pillar; 17. a limiting column; 18. a second guide surface; 19. a membrane ring; 21. a ring groove; 22. a pore; 23. a first guide surface; 24. a second mounting hole; 25. the groove is embedded.

Detailed Description

The present application is described in further detail below with reference to figures 1-3.

The embodiment of the application discloses a purification device for purifying glycoprotein. Referring to fig. 1 and 2, a purification device for purifying glycoprotein comprises a material pipe 2 provided with a cavity 1, a heat preservation pipe 3 sleeved on the material pipe 2, an end cover 4 arranged at one end of the material pipe 2, and a liquid discharge pipe 5 penetrating through the other end of the material pipe 2, wherein a filter membrane 6 is laid at the bottom of the cavity 1 far away from the end cover 4 of the material pipe 2, anion exchange resin 7 is filled in the cavity 1 of the material pipe 2, and a liquid inlet pipe 8 penetrates through the end cover 4. When the primary product is purified, the primary product is injected into the chamber 1 through the liquid inlet pipe 8, the primary product is contacted with the anion exchange resin 7, the anion exchange resin 7 adsorbs impurities in the primary product, and the purified product is discharged from the liquid discharge pipe 5 through the filter membrane 6, so that the process of purifying the glycoprotein is completed.

Referring to fig. 1 and 3, insulating tube 3 has seted up the jack 10 that link up in insulating tube 3's both ends, has seted up on the pore wall of jack 10 and has inlayed and establish groove 25, inlays to establish that the groove 25 is embedded to be established and to bond and have heat preservation 9, and material pipe 2 pegs graft in jack 10, and insulating tube 3 is from the one pot head that material pipe 2 kept away from end cover 4 and establish to material pipe 2 on, and the both ends of insulating tube 3 bond respectively in the both ends of material pipe 2.

The discharge pipe 5 is located at the end of the material pipe 2 remote from the end cap 4. The end, far away from the end cover 4, of the material pipe 2 is provided with a first mounting hole 11, the liquid discharge pipe 5 is inserted into the first mounting hole 11, the material pipe 2 and the liquid discharge pipe 5 are both made of glass, and the joint of the liquid discharge pipe 5 and the material pipe 2 is bonded through glass cement to achieve a waterproof effect.

The liquid discharge pipe 5 is provided with a pin shaft 12 for controlling the on-off of the liquid discharge pipe 5. The middle part of the liquid discharge pipe 5 is provided with a convex column 13, the convex column 13 is vertical to the liquid discharge pipe 5, and the convex column 13 is integrally formed on the liquid discharge pipe 5. The convex column 13 is provided with through holes 14 penetrating through two ends of the convex column 13, the through holes 14 are communicated with the liquid discharge pipe 5, the pin shaft 12 is inserted in the through holes 14 of the convex column 13, the outer wall of the pin shaft 12 is provided with a liquid discharge hole 15, the liquid discharge hole 15 and the liquid discharge pipe 5 are coaxially arranged, and the inner diameter of the liquid discharge hole 15 is smaller than that of the liquid discharge pipe 5.

The pin 12 is axially positioned on the boss 13. The pin shaft 12 is in a round table shape, the cross section of the pin shaft 12 is in an isosceles trapezoid shape, and the through hole 14 of the convex column 13 is matched with the pin shaft 12 in shape. After the pin 12 is inserted into the through hole 14, a pillar 16 is welded at one end of the pin 12, and the pillar 16 and the pin 12 are coaxially arranged. One end of the support block, which is far away from the convex column 13, is welded with a limiting column 17, and the end surface of the limiting column 17, which faces the pin shaft 12, is abutted against the end surface of the convex column 13, which is far away from the liquid discharge pipe 5. The outer diameter of the limiting column 17 is larger than the aperture of the through hole 14.

One end of the liquid discharge pipe 5, which is far away from the material pipe 2, is provided with a second guide surface 18, so that one end of the liquid discharge pipe 5, which is far away from the material pipe 2, is inclined, the cross section of the end is triangular, and the probability that liquid film is formed at the pipe orifice of the liquid discharge pipe 5 by residual liquid so that the residual liquid is difficult to drip is reduced.

Referring to fig. 1 and 2, a membrane ring 19 is arranged at one end of the material pipe 2 close to the liquid discharge pipe 5, and a filter membrane 6 is fixed on the membrane ring 19. The filter 6 is in the form of a disk, and the outer peripheral side of the filter 6 is bonded to the inner wall of the membrane ring 19. One end of the material pipe 2 close to the liquid discharge pipe 5 is provided with a ring groove 21, and the film ring 19 is clamped in the ring groove 21.

A plurality of holes 22 are uniformly formed in the end face of the filter membrane 6, the aperture of each hole 22 is smaller than the outer diameter of the anion exchange resin 7, the probability that the anion exchange resin 7 is discharged from the liquid discharge pipe 5 is reduced, and the aperture of each hole 22 is larger than the outer diameter of glycoprotein required to be purified by a manufacturer, so that purified substances can smoothly pass through the filter membrane 6 and then be discharged from the liquid discharge pipe 5.

The membrane ring 19 is made of rubber and has elasticity, and when the membrane ring 19 is installed, the membrane ring 19 is pressed to deform the membrane ring 19, and the filter membrane 6 is deformed accordingly and is not broken. The membrane ring 19 after the deformation is inserted from the material pipe 2 and is far away from one end of the liquid discharge pipe 5 until the membrane ring 19 moves to the annular groove 21, the extruded membrane ring 19 resets, the membrane ring 19 is clamped in the annular groove 21, and the outer wall of the membrane ring 19 abuts against the wall of the annular groove 21.

Referring to fig. 1 and 2, the end cap 4 is located at the end of the pipe 2 remote from the discharge pipe 5. The end cap 4, facing the feed tube 2, is provided with a first guide surface 23, which first guide surface 23 is inclined towards the filter membrane 6. When the end cover 4 is installed, the first guide surface 23 abuts against the orifice of the material pipe 2, and then the end cover 4 is pressed towards the material pipe 2, so that the end cover 4 is inserted into the cavity 1 of the material pipe 2 in an interference manner. The end cover 4 is provided with a second mounting hole 24 arranged along the axis direction of the material pipe 2, the liquid inlet pipe 8 is inserted into the second mounting hole 24 and communicated with the cavity 1, and the liquid inlet pipe 8 is bonded with the end cover 4 through glass cement to realize the waterproof effect.

The implementation principle of the purification device for purifying the glycoprotein in the embodiment of the application is as follows: when filtering the initial product, the initial product pours into the cavity 1 of material pipe 2 into from feed liquor pipe 8 into, and initial product and anion exchange resin 7 contact, and impurity in the initial product adsorbs in anion exchange resin 7, and the purification thing then discharges from fluid-discharge tube 5 via filter membrane 6, and the factory accessible twists round pin axle 12 and controls the break-make of fluid-discharge tube 5.

The above embodiments are preferred embodiments of the present application, and the protection scope of the present application is not limited by the above embodiments, so: all equivalent changes made according to the structure, shape and principle of the present application shall be covered by the protection scope of the present application.

Claims (10)

1. A purification apparatus for purifying a glycoprotein, characterized in that: the device comprises a material pipe (2), a cavity (1) is formed in the material pipe (2), anion exchange resin (7) is filled in the cavity (1) of the material pipe (2), a liquid discharge pipe (5) penetrates through one end, far away from a cavity opening of the cavity (1), of the material pipe (2), and a filter membrane (6) is arranged on the side wall, close to the liquid discharge pipe (5), of the cavity (1).

2. A purification apparatus for purifying a glycoprotein according to claim 1, wherein: an end cover (4) is arranged at one end, far away from the filter membrane (6), of the material pipe (2), and a material conveying pipe (2) communicated with the cavity (1) penetrates through the end cover (4).

3. A purification apparatus for purifying a glycoprotein according to claim 2, wherein: the material pipe (2) is sleeved with a heat insulation pipe (3), a heat insulation layer (9) is arranged on the inner wall of the heat insulation pipe (3), and the heat insulation layer (9) is tightly abutted to the outer wall of the material pipe (2).

4. A purification apparatus for purifying a glycoprotein according to claim 3, wherein: the heat preservation pipe (3) is provided with insertion holes (10) penetrating through two ends, the end cover (4) is inserted into the insertion holes (10) in an interference mode, and a first guide surface (23) inclining towards the filter membrane (6) is arranged at one end, facing towards the filter membrane (6), of the end cover (4).

5. A purification apparatus for purifying a glycoprotein according to claim 4, wherein: the periphery of the filter membrane (6) is fixed with a membrane ring (19), and the material pipe (2) is provided with a ring groove (21) for clamping the membrane ring (19).

6. A purification apparatus for purifying a glycoprotein according to claim 5, wherein: the membrane ring (19) is made of rubber.

7. A purification apparatus for purifying a glycoprotein according to claim 6, wherein: the filter membrane (6) comprises a plurality of pores (22), the pore size of the pores (22) is smaller than the outer diameter of the anion exchange resin (7) and the pore size of the pores (22) is larger than the outer diameter of the purified product.

8. The purification apparatus for purifying a glycoprotein according to claim 7, wherein: the liquid discharge pipe (5) is provided with a convex column (13) in a penetrating mode, one end of the convex column (13) is provided with a through hole (14), a pin shaft (12) is rotatably inserted into the through hole (14), and the pin shaft (12) is provided with a liquid discharge hole (15) which is coaxial with the liquid discharge pipe (5).

9. The purification apparatus for purifying a glycoprotein according to claim 8, wherein: the axial cross section of the pin shaft (12) is isosceles trapezoid, a limiting column (17) is fixed at one end of the pin shaft (12), and the limiting column (17) is abutted to the convex column (13).

10. The purification apparatus for purifying a glycoprotein according to claim 9, wherein: and a second guide surface (18) is arranged at one end of the liquid discharge pipe (5) far away from the material pipe (2).

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