CN214703652U - Card for detecting 6-benzyladenine colloidal gold in bean sprouts - Google Patents
Card for detecting 6-benzyladenine colloidal gold in bean sprouts Download PDFInfo
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- CN214703652U CN214703652U CN202120290473.5U CN202120290473U CN214703652U CN 214703652 U CN214703652 U CN 214703652U CN 202120290473 U CN202120290473 U CN 202120290473U CN 214703652 U CN214703652 U CN 214703652U
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- colloidal gold
- pad
- benzyladenine
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- bean sprouts
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Images
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The utility model relates to an immunology detects technical field, specifically is a 6-benzyl adenine colloidal gold detects card in bean sprouts, including casing and colloidal gold test paper strip, its characterized in that: the colloidal gold test strip is arranged inside the shell; the colloidal gold test strip comprises a support, a sample pad, a colloidal gold pad, a nitrocellulose membrane and a water absorption pad; the upper surface of the support is sequentially provided with a sample pad, a colloidal gold pad, a nitrocellulose membrane and a water absorption pad; the nitrocellulose membrane is sequentially provided with a detection line T and a quality control line C; the detection line T is coated with a 6-benzyladenine-carrier protein conjugate, and the quality control line C is coated with a goat anti-mouse secondary antibody. The utility model discloses sensitivity is high, the interference killing feature is strong, owing to adopt is that the good monoclonal antibody mark colloidal gold of 6-benzyl adenine sensitivity high specificity, has improved the sensitivity of detection like this and the interference killing feature when detecting different samples. The sensitivity can reach 0.02mg/kg and meets the national standard requirement.
Description
Technical Field
The utility model relates to an immunology detects technical field, specifically is a 6-benzyl adenine colloidal gold detects card in bean sprouts.
Background
The 6-benzyl adenine is an artificially synthesized plant growth hormone and is mainly used as a growth promoter and an antistaling agent. Can promote the physiological functions of seed germination, germ cell division, radicle growth inhibition and the like, is widely used in bean sprout production, has no fibrous root in the bean sprout cultivated by using the medicament in the production process of the bean sprout, has bright and full appearance, is difficult to deteriorate and can shorten the growth cycle of the bean sprout.
6-benzyl adenine is weak in accumulated toxicity, so that the esophagus and the gastric mucosa are damaged by excessive ingestion of people, the phenomena of nausea, vomit and the like are caused, the phenomenon of 'toxic bean sprout events' is frequently reported in recent years, and the '11 th publication in 2015' issued by the original national committee for health and family planning jointly stipulates that 6-benzyl adenine is not used in the production process of bean sprouts, and the detection limit of 6-benzyl adenine in GB/T23381-.
At present, the detection method of 6-benzyladenine mainly comprises high performance liquid chromatography and liquid chromatography-tandem mass spectrometry, the devices are expensive and have high detection cost, and the operation of the devices needs professional technicians, so that a detection card of 6-benzyladenine colloidal gold in bean sprouts is provided.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a 6-benzyl adenine colloidal gold detects card in bean sprouts to solve the problem that provides among the above-mentioned background art.
In order to achieve the above object, the utility model provides a following technical scheme:
the utility model provides a 6-benzyl adenine colloidal gold detects card in bean sprouts, includes casing and colloidal gold test paper strip, its characterized in that: the colloidal gold test strip is arranged inside the shell;
the colloidal gold test strip comprises a support, a sample pad, a colloidal gold pad, a nitrocellulose membrane and a water absorption pad;
the upper surface of the support is sequentially provided with a sample pad, a colloidal gold pad, a nitrocellulose membrane and a water absorption pad;
the nitrocellulose membrane is sequentially provided with a detection line T and a quality control line C;
the detection line T is coated with a 6-benzyladenine-carrier protein conjugate, and the quality control line C is coated with a goat anti-mouse secondary antibody.
Preferably, the casing is including epitheca and inferior valve, the upper surface of inferior valve is provided with the colloidal gold test paper strip, the top of inferior valve is fixed in the top of colloidal gold test paper strip and is provided with the epitheca, the sample loading port has been seted up on one side that is close to the sample pad on the surface of epitheca, the observation window has been seted up on the surface of epitheca.
Preferably, one side of the observation window is sequentially provided with a T word and a C word, and the T word and the C word respectively correspond to the detection line T and the quality control line C.
Preferably, the material of the support is a PVC plate, and the material of the sample pad is glass fiber.
Preferably, the position of the shell is made of hard plastic which does not absorb water.
Compared with the prior art, the beneficial effects of the utility model are that:
1. the detection card for 6-benzyladenine colloidal gold in bean sprouts is high in sensitivity and strong in anti-interference capability, and the monoclonal antibody with high sensitivity and good specificity of 6-benzyladenine is adopted to mark the colloidal gold, so that the detection sensitivity and the anti-interference capability in the detection of different samples are improved, and the sensitivity can reach 0.02mg/kg and meets the national standard requirements.
2. The detection card for 6-benzyladenine colloidal gold in bean sprouts is simple and convenient to operate, 60-80ul of treated sample extracting solution is sucked and added into the sample adding space of the detection card for 5-8min to observe the result.
3. The detection card for 6-benzyladenine colloidal gold in bean sprouts has the advantages that the displayed detection result is visual, intuitive and accurate, the color development of the T line of the detection test strip is compared with the color development of the C line of the quality control line to judge whether the bean sprouts are negative or positive, when the color development of the T line of the detection test strip is obviously deeper than the color development of the C line of the quality control line or the color development of the T line of the detection test strip is basically consistent with the color development of the C line of the quality control line, the judgment is negative, and when the color development depth of the T line of the detection test strip is obviously weak and the color development of the C line of the quality control line or the detection line is not colored and the C line of the quality control line is colored, the judgment is positive.
4. The test card for detecting 6-benzyladenine colloidal gold in bean sprouts is good in specificity, and the test paper strip is not crossed with 4-chlorobenxoxyacetic acid, gibberellin and other medicines.
5. This 6-benzyl adenine colloidal gold detects card in bean sprouts, with low costs, easily basic unit promotes, uses this detection card, need not the experiment and establishes the detection personnel with the specialty.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the observation window of the present invention;
FIG. 3 is a schematic diagram of the colloidal gold test strip of the present invention showing positive judgment;
FIG. 4 is a schematic diagram of the colloidal gold test strip of the present invention showing negative judgment;
fig. 5 is a schematic diagram of the colloidal gold test strip of the present invention becoming invalid.
In the figure: 1. a housing; 11. an upper shell; 12. a lower case;
2. a colloidal gold test strip; 21. a support; 22. a sample pad; 23. a colloidal gold pad; 24. a nitrocellulose membrane; 25. a water absorbent pad;
241. detecting a line T; 242. a quality control line C;
3. a sample addition port; 4. an observation window; 5. t word; 6. and C word.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.
In the description of this patent, it is noted that unless otherwise specifically stated or limited, the terms "mounted," "connected," and "disposed" are to be construed broadly and can include, for example, fixedly connected, disposed, detachably connected, disposed, or integrally connected and disposed. The specific meaning of the above terms in this patent may be understood by those of ordinary skill in the art as appropriate.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.
Referring to fig. 1-5, the present invention provides a technical solution:
the utility model provides a 6-benzyl adenine colloidal gold detects card in bean sprouts, includes casing 1 and colloidal gold test paper strip 2, its characterized in that: the colloidal gold test strip 2 is arranged inside the shell 1;
the colloidal gold test strip 2 comprises a support 21, a sample pad 22, a colloidal gold pad 23, a nitrocellulose membrane 24 and a water absorption pad 25;
the upper surface of the support 21 is provided with a sample pad 22, a colloidal gold pad 23, a nitrocellulose membrane 24 and a water absorption pad 25 in sequence;
the nitrocellulose membrane 24 is sequentially provided with a detection line T241 and a quality control line C242;
the detection line T241 is coated with 6-benzyladenine-carrier protein conjugate, and the quality control line C242 is coated with goat anti-mouse secondary antibody.
In this embodiment, preferably, the casing 1 includes an upper casing 11 and a lower casing 12, the colloidal gold test strip 2 is disposed on the upper surface of the lower casing 12, the upper casing 11 is fixedly disposed on the top of the lower casing 12 above the colloidal gold test strip 2, the sample addition port 3 is disposed on one side of the surface of the upper casing 11, which is close to the sample pad 22, and the observation window 4 is disposed on the surface of the upper casing 11.
In this embodiment, preferably, a T-word 5 and a C-word 6 are sequentially disposed on one side of the observation window 4, and the T-word 5 and the C-word 6 respectively correspond to the detection line T241 and the quality control line C242.
In this embodiment, the material of the support 21 is preferably a PVC plate, and the material of the sample pad 22 is preferably glass fiber.
In this embodiment, the position of the housing 1 is preferably made of a hard plastic that does not absorb water.
The working principle is as follows:
preparation of 6-benzyladenine monoclonal antibody-colloidal gold compound and preparation of colloidal gold
Adding 300ml of ultrapure water into a cleaned 500ml triangular flask, placing the triangular flask on a constant-temperature heating magnetic stirrer, heating the triangular flask to boiling, adding 3ml of chloroauric acid solution with the concentration of 1%, boiling again, adding 4.5ml of trisodium citrate dihydrate solution with the concentration of 1%, continuing heating the triangular flask until the triangular flask is boiled, stopping heating the triangular flask after 20min, recovering the triangular flask to room temperature, adding ultrapure water to recover the volume of the triangular flask, and storing the triangular flask at 4 ℃ for later use.
Labeling of 6-benzyladenine monoclonal antibody colloidal gold, preparation of 6-benzyladenine monoclonal antibody-colloidal gold complex
Adjusting the pH of the colloidal gold solution to 6.5 by 0.2mol/L potassium carbonate under the stirring of a magnetic stirrer, adding 4 micrograms of 6-benzyladenine monoclonal antibody into each milliliter of the colloidal gold solution, stirring at room temperature for 60min, adding 10% BSA solution to the final concentration of 1%, continuing stirring at room temperature for 60min, after stopping stirring, centrifuging at the temperature of 4 ℃ for 20min at the speed of 5500r/min, collecting the sediment at the bottom of the centrifuge tube, centrifuging at the temperature of 4 ℃ for 30min at the speed of 7500r/min for the upper layer of the colloidal gold solution, collecting the sediment at the bottom of the centrifuge tube, discarding the supernatant, redissolving the collected colloidal gold sediment twice by using a redissolving solution according to the volume of 1/20 of the colloidal gold solution when the colloidal gold solution is marked, storing at the temperature of 4 ℃ for later use, wherein the redissolving solution is a Tris-HCL buffer solution with the pH of 10mM and 8.2, wherein the BSA content is 1%, the Tween-20 is 0.1%, 0.3 percent of PVP40, 5 percent of trehalose and 5 percent of sucrose;
preparation of colloidal gold pad
Spraying the prepared 6-benzyladenine monoclonal antibody-colloidal gold compound on glass fiber according to the gold spraying amount of 2.0ul/cm, drying in a blast drying oven at 37 ℃ for 3h, and then drying, sealing and storing;
preparation of sample pad
Soaking the glass fiber in a sample pad treatment solution for 30min, drying in a blast drying oven at 37 ℃, sealing and storing for later use, wherein the sample pad treatment solution is a Tris-HCL solution with 0.02mol/L and pH of 8.2, the content of BSA is 1%, the content of casein is 0.1%, the content of Tween-20 is 0.3%, the content of PVP-40 is 0.1%, and the content of PC-300 is 0.1%;
coating of detection line and quality control line
Diluting 6-benzyladenine carrier protein antigen with 0.01mol/L phosphate solution with pH of 7.2 to the concentration of 0.3mg/ml, scribing on a nitrocellulose membrane according to the amount of 0.08ul/cm, diluting goat anti-mouse polyclonal antibody with 0.01mol/L phosphate solution with pH of 7.2 to the concentration of 0.5mg/ml, scribing on the nitrocellulose membrane according to the amount of 0.08ul/cm, drying the coated membrane in a blast drying oven at 37 ℃ for overnight, drying, sealing and storing for later use;
assembly of colloidal gold test card
Sequentially adhering the prepared sample pad, the gold label pad, the nitrocellulose membrane and the absorbent paper on a PVC (polyvinyl chloride) base plate, cutting the sample pad, the gold label pad, the nitrocellulose membrane and the absorbent paper into colloidal gold test strips with the width of 3mm by using a cutting machine, wherein the colloidal gold test strips are arranged in a lower shell, an upper shell is buckled on the lower shell, and the assembled detection card is placed in an aluminum foil bag with a drying agent for sealed storage;
use of test cards
Processing of the sample: shearing root tissues of mung bean sprouts or soybean sprouts, which are about 1cm long, by using scissors, putting the root tissues into a test tube or a mortar, and forcibly extruding liquid out; the liquid squeezed out from the tissue at the root of the bean sprouts is a liquid sample to be detected;
adding 60-80ul of the solution to be detected into the sample adding space of the detection card by using a liquid transfer gun, standing for 5-8min, and then interpreting the result;
interpretation of test results
When the detected sample contains 6-benzyladenine medicine, the color development depth of the detection line T line of the detection card is obviously weaker than that of the quality control line C line or the detection line T line is not developed, and the quality control line C line is developed; when the detected sample does not contain 6-benzyladenine, the color development depth of the detection line T line of the detection card is obviously deeper than the color development depth of the quality control line C line or the color development depth of the detection line T is basically consistent with the color development of the quality control line C. When only the detection line T line of the detection card is colored or neither the detection line T line nor the quality control line C of the detection card is colored, the detection card is invalid.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It should be understood by those skilled in the art that the present invention is not limited by the above embodiments, and the description in the above embodiments and the description is only preferred examples of the present invention, and is not intended to limit the present invention, and that the present invention can have various changes and modifications without departing from the spirit and scope of the present invention, and these changes and modifications all fall into the scope of the claimed invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (5)
1. The utility model provides a 6-benzyl adenine colloidal gold detects card in bean sprouts, includes casing (1) and colloidal gold test paper strip (2), its characterized in that: the colloidal gold test strip (2) is arranged inside the shell (1);
the colloidal gold test strip (2) comprises a support (21), a sample pad (22), a colloidal gold pad (23), a nitrocellulose membrane (24) and a water absorption pad (25);
the upper surface of the support (21) is sequentially provided with a sample pad (22), a colloidal gold pad (23), a nitrocellulose membrane (24) and a water absorption pad (25);
the nitrocellulose membrane (24) is sequentially provided with a detection line T (241) and a quality control line C (242);
the detection line T (241) is coated with a 6-benzyladenine-carrier protein conjugate, and the quality control line C (242) is coated with a goat anti-mouse secondary antibody.
2. The card for detecting 6-benzyladenine colloidal gold in bean sprouts according to claim 1, wherein: casing (1) is including epitheca (11) and inferior valve (12), the upper surface of inferior valve (12) is provided with colloidal gold test paper strip (2), the top of inferior valve (12) is fixed in the top of colloidal gold test paper strip (2) and is provided with epitheca (11), sample addition mouth (3) have been seted up in one side that is close to sample pad (22) on the surface of epitheca (11), observation window (4) have been seted up on the surface of epitheca (11).
3. The card for detecting 6-benzyladenine colloidal gold in bean sprouts according to claim 2, wherein: one side of the observation window (4) is sequentially provided with T words (5) and C words (6), and the T words (5) and the C words (6) correspond to the detection line T (241) and the quality control line C (242) respectively.
4. The card for detecting 6-benzyladenine colloidal gold in bean sprouts according to claim 1, wherein: the material of the support (21) is a PVC plate, and the material of the sample pad (22) is glass fiber.
5. The card for detecting 6-benzyladenine colloidal gold in bean sprouts according to claim 1, wherein: the position of the shell (1) is made of non-absorbent hard plastic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120290473.5U CN214703652U (en) | 2021-02-02 | 2021-02-02 | Card for detecting 6-benzyladenine colloidal gold in bean sprouts |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120290473.5U CN214703652U (en) | 2021-02-02 | 2021-02-02 | Card for detecting 6-benzyladenine colloidal gold in bean sprouts |
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| Publication Number | Publication Date |
|---|---|
| CN214703652U true CN214703652U (en) | 2021-11-12 |
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| CN202120290473.5U Expired - Fee Related CN214703652U (en) | 2021-02-02 | 2021-02-02 | Card for detecting 6-benzyladenine colloidal gold in bean sprouts |
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| Country | Link |
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| CN (1) | CN214703652U (en) |
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