CN214694238U - Liquid return device for stem cell perfusion culture - Google Patents
Liquid return device for stem cell perfusion culture Download PDFInfo
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- CN214694238U CN214694238U CN202020867073.1U CN202020867073U CN214694238U CN 214694238 U CN214694238 U CN 214694238U CN 202020867073 U CN202020867073 U CN 202020867073U CN 214694238 U CN214694238 U CN 214694238U
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Abstract
The utility model provides a stem cell perfusion is cultivateed with returning liquid device, including funnel oral area, funnel neck, filter screen, sealed cutting ferrule, can alleviate and avoid stem cell perfusion to cultivate the jam of in-process stem cell to the back flow filter screen, effectively guarantee going on smoothly that stem cell perfusion cultivates.
Description
Technical Field
The utility model belongs to the technical field of stem cell culture and specifically relates to a stem cell perfusion is cultivateed and is used liquid device that returns is related to.
Background
The stem cells have a variety of types and are widely applied in the fields of disease treatment, medical and beauty treatment, anti-aging, drug screening and the like. Stem cell culture has become a bottleneck for the development of the whole stem cell industry chain, and stem cell culture devices have become hot spots for the development of the stem cell culture field.
Perfusion culture can provide nutrients for the growth of the stem cells in time, take away metabolic waste of the stem cells in time and effectively improve the culture efficiency of the stem cells. In order to prevent the stem cells from flowing out during perfusion culture, a filter screen is usually installed on the backflow pipeline to block the stem cells. Traditional backward flow filter screen is settled inside the culture solution, and this kind is installed dual drawback, and firstly stem cell striking filter screen causes the stem cell to damage in stirring in-process, and secondly the backward flow flows in-process filter screen interception stem cell and makes the stem cell adsorb in the jam that the filter screen surface caused the filter screen. The stem cell perfusion culture process is often forced to be interrupted or even stopped due to blockage. At present, effectively avoiding the blockage of a culture solution return pipe becomes a key factor for restricting perfusion culture.
Disclosure of Invention
The utility model provides a stem cell perfusion is cultivateed with returning liquid device, its material utilizes gravity, the rotatory shearing force of culture solution, reduces and eliminates the absorption of stem cell on the filter screen, reaches the continuous backward flow of perfusion culture in-process culture solution. In the stem cell culture process, the stem cells are subjected to the dual effects of stirring force and gravity and are distributed in the culture solution in a gradient manner, the concentration of the stem cells at the lower part is higher than that at the upper part, and the concentration of the stem cells at the top of the culture solution is the lowest. Set up reflux unit can effectively alleviate the jam of filter screen at the position that stem cell concentration is minimum, even make a small amount of stem cells adsorb, the stirring power also can make adsorbed stem cells break away from the filter screen with the dual function of gravity, has effectively prevented the jam of perfusion culture in-process filter screen, can guarantee going on smoothly of stem cell perfusion culture.
The utility model discloses a concrete technical scheme is: the liquid returning device for stem cell perfusion culture is composed of four parts: the culture medium backflow prevention device comprises a funnel mouth, a funnel neck, a filter screen and a sealing clamping sleeve, wherein the filter screen is connected with the funnel mouth, the aperture of the filter screen is between 10 nanometers and 1 millimeter, and the filter screen and the mouth of the funnel can ensure that a culture solution can only flow back through the filter screen; the sealing clamp is sleeved on the funnel neck, so that the connection part is kept sealed, the culture system is sealed, the culture solution can effectively flow back, and the funnel neck can move up and down without damaging the seal. The specific position of the whole device in the stem cell culture tank is adjusted through the sealing clamping sleeve, the filter screen is guaranteed to be positioned on the surface of the culture solution, and perfusion culture requirements of different culture volumes can be met. In the stem cell culture process, the culture solution in the stem cell culture tank enters the funnel opening through the filter screen by the pressure formed by the culture solution input, and flows out of the stem cell culture tank through the funnel neck, so that the perfusion culture of the stem cells is completed.
The beneficial effects of the utility model are that can alleviate and avoid stem cell perfusion to cultivate the jam of in-process stem cell to the back flow filter screen, effectively guarantee going on smoothly that stem cell perfusion cultivates.
Drawings
FIG. 1 is a schematic view (sectional view) of the overall structure of a liquid returning device for stem cell perfusion culture
FIG. 2 is a schematic view of an assembly effect
In the figure: stirring shaft 11 sensor group 12 with paddle for 1 funnel neck, 2 filter screens, 3 sealing clamping sleeves, 4 stem cell culture tank clamping sleeves, 5 stem cell culture tank clamping sleeves, 6 culture tank top covers, 7 culture tank bottom plates, 8 liquid inlet pipes, 9 sealing shaft sleeves and 10 culture tank top covers of funnels
Detailed Description
Pre-assembling the parts of the stem cell culture tank except the culture tank top cover 7, wherein the parts comprise an installation sealing shaft sleeve 10, a stirring shaft 11 with blades, a sensor group 12 and a liquid inlet pipe 9; the filter screen 3 is connected with the funnel mouth 1, the connection part keeps sealed, the sealing cutting sleeve 4 is arranged on the funnel neck 2 and can move up and down, the connection part keeps sealed, the specific position of the whole device in the stem cell culture tank is adjusted through the sealing cutting sleeve 4, the filter screen 3 is ensured to be positioned on the surface of the culture solution, and perfusion culture requirements of different culture volumes can be met; the stem cell culture tank jacket 5 is assembled with the culture tank bottom plate 8. And (3) hermetically assembling the pre-assembled stem cell culture tank top cover 7 and the stem cell culture tank body 6, and primarily finishing the assembly of the stem cell culture device. The whole system is closed, and the continuous perfusion culture is effectively ensured.
The stem cell seeds and fresh culture solution are conveyed into a stem cell culture tank through a liquid inlet pipe 9, and stem cell culture is started. Culture solution is added into the stem cell culture tank body 6, and because the filter screen 3 is the only outlet of the stem cell culture tank, the newly input culture solution can push the original culture solution in the stem cell culture tank into the filter screen 3 and flow out of the stem cell culture tank through the funnel mouth part 1 and the funnel neck 2. Under the dual functions of stirring and gravity, the distribution amount of stem cells in the culture solution in the stem cell culture tank body 6 on the top is minimum; the aperture of filter screen 3 is less than the stem cell, and the stem cell can not get into filter screen 3, and the stem cell that adsorbs on filter screen 3 of a very small amount receives gravity and the effect of rotatory liquid stream also can break away from filter screen 3, greatly reduces the jam of stem cell to filter screen 3, and the filter screen jam problem obtains fine solution, effectively guarantees going on of stem cell perfusion culture.
The present embodiments are intended to embrace all such alternatives, modifications and variances which fall within the broad scope of the appended claims. Therefore, any omission, modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included within the protection scope of the present invention.
Claims (1)
1. A liquid return device for stem cell perfusion culture is characterized by comprising a funnel opening part, a funnel neck, a filter screen and a sealing clamping sleeve; the filter screen is connected with the opening of the funnel, the aperture of the filter screen is between 10 nanometers and 1 millimeter, the connection part of the filter screen and the opening of the funnel is kept sealed, and the culture solution can only complete the backflow through the filter screen; the sealing clamp is sleeved on the funnel neck, so that the connection part is kept sealed, the culture system is sealed, the culture solution can effectively flow back, and the funnel neck can move up and down without damaging the seal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020867073.1U CN214694238U (en) | 2020-05-18 | 2020-05-18 | Liquid return device for stem cell perfusion culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020867073.1U CN214694238U (en) | 2020-05-18 | 2020-05-18 | Liquid return device for stem cell perfusion culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214694238U true CN214694238U (en) | 2021-11-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020867073.1U Active CN214694238U (en) | 2020-05-18 | 2020-05-18 | Liquid return device for stem cell perfusion culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214694238U (en) |
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2020
- 2020-05-18 CN CN202020867073.1U patent/CN214694238U/en active Active
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