CN214694180U - Multichannel unicellular nucleic acid processing instrument - Google Patents

Abstract

The utility model relates to a unicellular nucleic acid processing apparatus technical field discloses a multichannel unicellular nucleic acid processing apparatus, include: a frame; the moving assembly is arranged on the rack and comprises an X shaft assembly, a Y shaft assembly and at least two Z shaft assemblies, and the X shaft assembly can simultaneously drive the at least two Z shaft assemblies to move along the X shaft direction; the carrying platform is arranged at the output end of the Y shaft assembly and is used for placing the disposable suction tube and the chip; at least two air pumps, every air pump all set up the output of a Z axle subassembly and are located the top of microscope carrier, all fixedly on every air pump be provided with a magnet and a connecting piece, remove the subassembly and can drive the air pump and drive magnet and chip laminating, every connecting piece homoenergetic can stretch into in a disposable straw so that disposable straw is fixed on the connecting piece. The utility model discloses a multichannel unicellular nucleic acid processing apparatus, lower and can handle two kinds at least cells simultaneously to the requirement of gas tightness.

Description

Multichannel unicellular nucleic acid processing instrument

Technical Field

The utility model relates to a unicellular nucleic acid processing apparatus technical field especially relates to a multichannel unicellular nucleic acid processing apparatus.

Background

When the existing single cell nucleic acid processing instrument is used for extracting DNA or RNA in cells, different reagents need to be added into a chip in the extraction process, in the prior art, gas is generally introduced into corresponding reagent grooves, so that the reagents in the reagent grooves enter the chip, and the purpose of adding different reagents into the chip is realized.

SUMMERY OF THE UTILITY MODEL

Based on above, the utility model aims to provide a multichannel unicellular nucleic acid processing apparatus, lower and can handle two kinds at least cells simultaneously to the requirement of gas tightness.

In order to achieve the purpose, the utility model adopts the following technical proposal:

a multi-channel single-cell nucleic acid processing instrument, comprising: a frame; the moving assembly is arranged on the rack and comprises an X shaft assembly, a Y shaft assembly and at least two Z shaft assemblies, and the X shaft assembly can simultaneously drive the at least two Z shaft assemblies to move along the X shaft direction; the carrying platform is arranged at the output end of the Y-axis assembly and used for placing a disposable suction tube and a chip, and the Y-axis assembly can drive the carrying platform to move along the Y-axis direction; the air pump is arranged at the output end of the Z shaft assembly and located above the loading platform, the Z shaft assembly can drive the air pump to move along the Z shaft direction, a magnet and a connecting piece are fixedly arranged on the air pump, the moving assembly can drive the air pump to drive the magnet and the chip to be attached, and the connecting piece can extend into the disposable suction tube to enable the disposable suction tube to be fixed on the connecting piece.

As a preferred scheme of the multi-channel single-cell nucleic acid processing instrument, the multi-channel single-cell nucleic acid processing instrument further comprises at least two blocking frames arranged on the carrier, the at least two blocking frames are respectively arranged corresponding to the at least two connecting pieces one by one, the edges of the blocking frames are provided with blocking grooves, and the blocking grooves are used for blocking the disposable straws so as to enable the disposable straws to be separated from the connecting pieces.

As a preferable scheme of the multi-channel single-cell nucleic acid processing instrument, a pressure detection piece is arranged in each air pump.

As a preferred scheme of the multi-channel single-cell nucleic acid processing instrument, the Y-axis assembly comprises a Y-axis motor and a Y-axis ball screw, the output end of the Y-axis motor is connected with a Y-axis screw rod of the Y-axis ball screw, and a Y-axis nut of the Y-axis ball screw is connected with the carrier.

As a preferred scheme of the multi-channel single-cell nucleic acid processing instrument, the Y-axis assembly comprises a Y-axis motor, a Y-axis driving wheel, a Y-axis driven wheel and a Y-axis synchronous belt, the Y-axis driving wheel is arranged at the output end of the Y-axis motor, the Y-axis driven wheel is rotatably arranged on the rack, the Y-axis synchronous belt is in transmission connection with the Y-axis driving wheel and the Y-axis driven wheel respectively, the carrying platform is fixedly connected with the Y-axis synchronous belt, and the Y-axis motor can drive the Y-axis driving wheel to drive the Y-axis synchronous belt and the carrying platform to move along the Y-axis direction.

As an optimal scheme of multichannel unicellular nucleic acid processing instrument, the X axle subassembly includes X axle motor and X axle ball, the Z axle subassembly includes Z axle motor and Z axle ball, X axle motor stiff end sets up in the frame, the output of X axle motor with X axle ball's X axle screw links to each other, be equipped with on X axle ball's the X axle nut Z axle motor, the output of Z axle motor with Z axle ball transmission is connected, be equipped with on Z axle ball's the Z axle nut the air pump.

As a preferred scheme of the multi-channel single-cell nucleic acid processing instrument, the carrier comprises a connecting seat and at least two carrier bodies fixedly arranged on the connecting seat, the connecting seat is arranged at the output end of the Y shaft assembly, the at least two carrier bodies and the at least two Z shaft assemblies are arranged in a one-to-one correspondence manner, and each carrier body is provided with the disposable suction tube and the chip.

As a preferred scheme of the multi-channel single-cell nucleic acid processing instrument, one of the connecting seat and the carrier body is provided with a guide piece, and the other is provided with a guide hole matched with the guide piece.

As a preferred scheme of the multi-channel single-cell nucleic acid processing instrument, the connecting seat is connected with the carrier body through a bead screw, a mounting groove is formed in the connecting seat, the carrier body is placed in the mounting groove, the fixed end of the bead screw is arranged on the connecting seat and located at the end portion of the connecting seat, and the movable end of the bead screw is abutted against the carrier body so that the carrier body is positioned in the mounting groove.

As a preferred scheme of the multi-channel single-cell nucleic acid processing instrument, the connecting seat is connected with the carrier body through an elastic press-fit component, the elastic press-fit component is arranged on the connecting seat and located at the end of the connecting seat, the elastic press-fit component comprises a spring plate, and the spring plate can be pressed on the carrier body so that the carrier body is fixed on the carrier body.

The utility model has the advantages that: the utility model discloses a multichannel unicellular nucleic acid processing apparatus can draw DNA or RNA in two kind at least cells simultaneously, and is lower to the requirement of gas tightness, has accelerated the process of experiment, fixes magnet on the air pump can with the chip laminating for magnet is bigger to the adsorption affinity in the chip, has improved the success rate of experiment.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required to be used in the description of the embodiments of the present invention will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the contents of the embodiments of the present invention and the drawings without creative efforts.

FIG. 1 is a schematic diagram of a multi-channel single-cell nucleic acid processing apparatus according to an embodiment of the present invention in one direction;

FIG. 2 is a schematic diagram of a multi-channel single-cell nucleic acid processing apparatus according to another embodiment of the present invention.

In the figure:

1. a frame;

2. an X-axis assembly;

3. a Y-axis assembly; 31. a Y-axis motor; 32. a Y-axis ball screw;

4. a Z-axis assembly; 41. a Z-axis motor; 42. a Z-axis ball screw; 43. a Z-axis transport assembly; 431. a Z-axis driving wheel; 432. a Z-axis driven wheel; 433. a Z-axis synchronous belt;

5. an air pump; 6. a magnet; 7. a connecting member; 71. a connecting projection;

81. a connecting seat; 811. a guide member; 812. a mounting seat; 82. a stage body; 821. positioning the boss; 83. an elastic pressing component; 831. a spring plate; 832. a locking member;

91. a blocking frame; 910. a blocking groove; 92. a slide base; 920. a chute;

101. a Y-axis limit switch; 102. a Z-axis limit switch;

111. an X-axis guide rail; 112. a Y-axis guide rail; 113. a fixed seat; 1131. a Z-axis guide rail;

100. a disposable straw; 200. a chip; 300. a reagent tube; 400. a sample tube; 500. a waste liquid pipe.

Detailed Description

In order to make the technical problems, technical solutions and technical effects achieved by the present invention more clear, the embodiments of the present invention will be described in further detail with reference to the accompanying drawings, and obviously, the described embodiments are only some embodiments, not all embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by those skilled in the art without creative efforts belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Wherein the terms "first position" and "second position" are two different positions.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection or a removable connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

The embodiment provides a multi-channel unicellular nucleic acid processing instrument, as shown in fig. 1 and fig. 2, the multi-channel unicellular nucleic acid processing instrument comprises a rack 1, a moving assembly, a carrier, two air pumps 5, two magnets 6 and two connecting pieces 7, the moving assembly is arranged on the rack 1 and comprises an X-axis assembly 2, a Y-axis assembly 3 and two Z-axis assemblies 4, the X-axis assembly 2 can simultaneously drive the two Z-axis assemblies 4 to move along the X-axis direction, the carrier is arranged at the output end of the Y-axis assembly 3 and is used for placing a disposable pipette 100 and a chip 200, the Y-axis assembly 3 can drive the carrier to move along the Y-axis direction, each air pump 5 is arranged at the output end of one Z-axis assembly 4 and is located above the Z-axis assembly, each Z-axis assembly 4 can drive one air pump 5 to move along the Z-axis direction, one magnet 6 and one connecting piece 7 are fixedly arranged on each carrier 5, the moving assembly can drive the air pump 5 to drive the magnet 6 to be attached to the chip 200, each connector 7 is capable of extending into one of the disposable pipettes 100 to secure the disposable pipette 100 to the connector 7.

Specifically, the number of the X shaft assemblies 2 and the number of the Z shaft assemblies 4 in this embodiment are both one, one Y shaft assembly 3 can realize the movement of the stage along the Y shaft direction, one X shaft assembly 2 can simultaneously drive the movement of two Z shaft assemblies 4 along the X shaft direction, and each Z shaft assembly 4 can respectively drive one air pump 5 and the movement of the connecting member 7 and the magnet 6 corresponding to the air pump 5 along the Z shaft direction, so as to realize the treatment of two types of cells. In other embodiments, the number of the Z-axis assemblies 4 is not limited to two in this embodiment, and may also be three or more, each Z-axis assembly 4 is fixedly provided with one air pump 5, and each air pump 5 is fixedly provided with one magnet 6 and one connecting member 7.

Further, as shown in fig. 1, each of the connectors 7 of the present embodiment is provided with a connection protrusion 71, and the connection protrusion 71 can be engaged with the disposable pipette 100 so that the disposable pipette 100 is interference-fitted on the connector 7. The connecting piece 7 of this embodiment is the spliced pole, the diameter of spliced pole is less than the internal diameter of disposable straw 100, the number of connecting protrusion 71 is two, two connecting protrusion 71 intervals set up on connecting piece 7, one of them connecting protrusion 71 is located the lower extreme of spliced pole, so that insert in disposable straw 100 when connecting piece 7 begins to contact with disposable straw 100, the surface of connecting protrusion 71 is spherical, the diameter that spherical corresponds is greater than the internal diameter of disposable straw 100, so that disposable straw 100 can firmly be fixed on connecting piece 7. After the disposable pipette 100 is fixed to the connector 7, the upper connecting protrusion 71 is located inside the disposable pipette 100 and close to the opening of the disposable pipette 100, and when the air pump 5 exhausts or inhales air, the connecting protrusion 71 can prevent air from leaking out from the gap between the connector 7 and the disposable pipette 100 or external air from entering the connector 7 through the gap.

The Y-axis assembly 3 of the multi-channel single-cell nucleic acid processing instrument provided by the embodiment can drive the carrier to drive the disposable pipette 100 and the chip 200 to move along the Y-axis direction, the X-axis assembly 2 and the Z-axis assembly 4 can drive the two air pumps 5 to drive the two connecting pieces 7 to move along the X-axis direction and the Z-axis direction respectively, so as to realize the installation of the disposable pipette 100, the air pumps 5 can enable the disposable pipette 100 to suck reagents or add the reagents into the chip 200, the X-axis assembly 2 and the Z-axis assembly 4 can also drive the air pump 5 to drive the magnet 6 to move to be attached to the chip 200 so as to adsorb magnetic beads in the chip 200, so that each magnet 6 can be attached to one chip 200, the adsorption force of the magnet 6 to the magnetic beads is larger, the success rate of the experiment is higher, the multichannel single cell nucleic acid processing instrument has simpler structure, more convenient operation and lower requirement on air tightness.

The multichannel unicellular nucleic acid processing apparatus that this embodiment provided can draw DNA or RNA in two kinds of cells simultaneously, and is lower to the requirement of gas tightness, has accelerated the process of experiment, fixes magnet 6 on air pump 5 and can laminate with chip 200 for magnet 6 is bigger to the adsorption affinity in the chip 200, has improved the success rate of experiment.

As shown in FIGS. 1 and 2, the multi-channel single-cell nucleic acid processing apparatus of the present embodiment further includes two blocking racks 91, a slide base 92 and a recovery box (not shown) are disposed below each blocking rack 91, the blocking racks 91 are disposed on the slide base 92, as shown in FIG. 2, a blocking groove 910 is disposed at an edge of each blocking rack 91, the blocking groove 910 is used for blocking the disposable pipette 100 to separate the disposable pipette 100 from the connector 7, the slide base 92 is disposed below the blocking groove 910, as shown in FIG. 2, a slide slot 920 is disposed on the slide base 92, and the disposable pipette 100 separated from the connector 7 slides down from the slide slot 920 into the recovery box. Specifically, Y axle subassembly 3 drive platform moves along Y axle direction, X axle subassembly 2 and Z axle subassembly 4 drive air pump 5 respectively and drive connecting piece 7 along X axle direction and the motion of Y axle direction, so that disposable straw 100 fixes on connecting piece 7, realize the fixed of disposable straw 100, after the use, the removal subassembly can drive air pump 5 and drive the motion of disposable straw 100 after connecting piece 7 and the use, block that the groove 910 that blocks of frame 91 can block disposable straw 100, realize breaking away from of disposable straw 100 and connecting piece 7.

Further, the diameter corresponding to the spherical surface of the connection protrusion 71 is smaller than the width of the blocking groove 910, when extracting DNA or RNA from a cell, the connection member 7 extends into the disposable pipette 100 to fix the disposable pipette 100 on the connection member 7, the moving assembly first drives the disposable pipette 100 to suck a reagent from the reagent tube 300, then drops the reagent in the disposable pipette 100 into the chip 200, and finally removes the disposable pipette 100, when removing, the moving assembly drives the connection member 7 to extend into the blocking groove 910 and the disposable pipette 100 is located below the blocking frame 91, then the Z-axis assembly 4 of the moving assembly drives the connection member 7 to move upward, the upper end surface of the disposable pipette 100 gradually abuts against the lower surface of the blocking frame 91, because the width of the blocking groove 910 is smaller than the outer diameter of the disposable pipette 100, the disposable pipette 100 cannot move upward, and the Z-axis assembly 4 can continuously drive the connection member 7 to move upward, finally, the disposable pipette 100 falls from the connecting member 7, and the fallen disposable pipette 100 falls into the recycling bin through the chute 920, thereby recycling the used disposable pipette 100. In other embodiments, the blocking frame 91 can be directly arranged on the frame 1 or the carrying platform, the recycling bin is directly arranged under the blocking frame 91, the sliding seat 92 is not needed to be arranged, the disposable straw 100 separated from the connecting piece 7 can directly fall into the recycling bin, and the recycling of the disposable straw 100 is realized.

Preferably, the multi-channel single-cell nucleic acid processing apparatus of this embodiment further includes a controller (not shown in the figure), the controller is electrically connected to the moving assembly and the air pump 5, the controller may be a centralized or distributed controller, for example, the controller may be a single-chip microcomputer or may be formed by a plurality of distributed single-chip microcomputers, and the single-chip microcomputers may run a control program to further control the moving assembly and the air pump 5 to implement the functions thereof.

As shown in fig. 1, the stage of this embodiment includes a connection seat 81 and two stage bodies 82 fixedly disposed on the connection seat 81, the connection seat 81 is disposed at an output end of the Y shaft assembly 3, the two stage bodies 82 and the two Z shaft assemblies 4 are disposed in a one-to-one correspondence, and each stage body 82 is provided with a disposable suction tube 100 and a chip 200. Be equipped with guide 811 on connecting seat 81, be equipped with on microscope carrier body 82 with guide 811 complex guiding hole (not shown in the figure), guide 811 and guiding hole can play the effect of tentatively fixing a position microscope carrier body 82 on connecting seat 81, prevent that microscope carrier body 82 from moving in the horizontal direction for connecting seat 81.

Specifically, guide 811 of this embodiment is the guide pin, and the fixed setting of guide pin is on connecting seat 81 and the number of guide pin is two, and the number of guiding hole is two on microscope stage body 82, and two guide pins and two guiding holes one-to-one set up. In other embodiments, the number of the guiding elements 811 is not limited to two in this embodiment, and may be one, three or more than three, which is selected according to actual needs. In other embodiments, the carrier body 82 may be provided with a guide 811, and the connecting seat 81 may be provided with a guide hole engaged with the guide 811.

Connecting seat 81 and carrier body 82 of this embodiment link to each other through ripples pearl screw (not shown in the figure), are equipped with mounting groove (not shown in the figure) on connecting seat 81, and carrier body 82 places in the mounting groove, and the stiff end setting of ripples pearl screw is on connecting seat 81 and be located the tip of connecting seat 81, and the expansion end of ripples pearl screw and carrier body 82 butt are so that carrier body 82 fixes a position in the mounting groove. The ball screw positions the carrier body 82 in the mounting groove, so that the chip 200 in the placing groove of the carrier body 82 is fixed in position, the probability of movement of the chip 200 is reduced, the carrier cannot be toppled, and the probability of success of an experiment is increased.

Specifically, the number of the wave ball screws of the present embodiment is one, and one wave ball screw is respectively located at the end of the connection seat 81. In order to install the ripples pearl screw on connecting seat 81, be equipped with a mount pad 812 on connecting seat 81, the stiff end of ripples pearl screw is all fixed to be set up on mount pad 812, and the expansion end of ripples pearl screw all with microscope stage body 82 butt to fix microscope stage body 82 on connecting seat 81, the ripples pearl screw of this embodiment belongs to prior art, and the acquisition is bought outward to specific accessible, and here is no longer repeated. In other embodiments, the number of the wave ball screws may also be one or more than two, and is specifically set according to actual needs.

In this embodiment, each stage body 82 is provided with a placement groove (not shown), eight first placement holes (not shown), ten second placement holes (not shown), a third placement hole (not shown), a waste liquid hole (not shown), and a pick-and-place groove (not shown), the placement grooves are used for placing the chip 200, the chip 200 is disposed corresponding to the magnet 6 on the air pump 5, the first placement hole is used for placing the disposable pipette 100, the second placement hole is used for placing the reagent tube 300, the third placement hole is used for placing the sample tube 400, the waste liquid hole is used for placing the waste liquid tube 500, the stage body 82 is provided with a positioning boss 821, the second placement hole penetrates through the positioning boss 821, the positioning boss 821 is used for supporting the reagent tube 300, each of the four corners of the pick-and-place groove is provided with a placement groove, the pick-and-place groove is communicated with the placement grooves, the additional pick-and-place groove is convenient for placing the chip 200 in the placement grooves, and simultaneously, the operator can take out the chip 200 in the placing groove conveniently. In other embodiments, the number of the taking and placing slots is not limited to four in this embodiment, and may be two or three, specifically set according to actual needs.

In order to further fix the connecting seat 81 on the carrier body 82, as shown in fig. 1, the connecting seat 81 and the carrier body 82 are further connected by an elastic pressing component 83, and the elastic pressing component 83 is disposed on the connecting seat 81 and located at an end portion of the connecting seat 81, as shown in fig. 2, the elastic pressing component 83 includes an elastic sheet 831, and the elastic sheet 831 can be pressed on the carrier body 82 to position the carrier body 82 in the mounting groove. Specifically, the number of the elastic press-fit components 83 in this embodiment is four, two of the elastic press-fit components 83 are located at two opposite ends of one carrier body 82, and the other two elastic press-fit components 83 are located at two opposite ends of the other carrier body 82. The elastic pressing assembly 83 of the present embodiment further includes a locking member 832 and an elastic member (not shown in the figure), the elastic member is a spring, the locking member 832 penetrates through the connecting seat 81, the elastic sheet 831 and the elastic member, the elastic sheet 831 can rotate relative to the connecting seat 81, the elastic member is sleeved on the locking member 832 and located below the elastic sheet 831, one end of the elastic member abuts against a lower end of the locking member 832, and the other end of the elastic member abuts against the connecting seat 81.

The locking member 832 of the present embodiment includes a fastening member and three nuts, wherein one nut is screwed on the lower end of the fastening member, the elastic member abuts against the nut, the other two nuts are screwed on the fastening member and are located between the elastic sheet 831 and the connecting seat 81, and the elastic sheet 831 is clamped between the two nuts and the fixed end of the fastening member. When the elastic sheet 831 needs to be rotated, the fastener is pulled upwards, the fastener is rotated simultaneously, at the moment, the elastic sheet 831 and the three nuts rotate in the horizontal plane relative to the connecting seat 81 along with the fastener, the elastic piece is in a compression state, a gap exists between the nut below the elastic sheet 831 and the connecting seat 81, and therefore the elastic sheet 831, the fastener and the three nuts can rotate relative to the connecting seat 81, when the elastic sheet 831 abuts against the carrier body 52, the fastener is loosened, and the elastic piece can push the nuts to drive the fastener to reset. In other embodiments, only the wave ball screw may be disposed without the elastic pressing component 83, or only the elastic pressing component 83 may be disposed without the wave ball screw, specifically according to actual requirements.

The multi-channel single-cell nucleic acid processing apparatus of this embodiment further comprises two pressure detecting members (not shown), each of which is disposed in one of the air pumps 5. The pressure detection piece of this embodiment is pressure sensor, pressure sensor can real-time detection air pump 5 internal pressure, when removal subassembly drive air pump 5 and connecting piece 7 drive disposable straw 100 and draw reagent from reagent pipe 300, air pump 5 bleeds, reagent in the reagent pipe 300 gets into in the disposable straw 100, air pump 5, pressure in connecting piece 7 and the disposable straw 100 reduces along with the entering of reagent gradually, the pressure value that pressure sensor detected is the one-to-one with the capacity of the reagent in the disposable straw 100, therefore, can learn the total capacity of reagent in the disposable straw 100 according to the pressure value that pressure sensor detected, if disposable straw 100 draws the sample from sample pipe 400, specifically draw reagent from reagent pipe 300 together. The liquid-transfering structure that this embodiment provided can obtain the capacity of the reagent that disposable straw 100 absorbed according to the pressure value in the air pump 5 that pressure sensor detected for the experimental speed has been accelerated.

As shown in fig. 2, the Y-axis assembly 3 of the present embodiment includes a Y-axis motor 31 and a Y-axis ball screw 32, an output end of the Y-axis motor 31 is connected to a Y-axis screw of the Y-axis ball screw 32, a Y-axis nut of the Y-axis ball screw 32 is connected to a connecting seat 81, and the Y-axis motor 31 can drive the Y-axis ball screw 32 to drive the connecting seat 81 and the stage body 82 to move along the Y-axis direction. In order to ensure that the Y-axis assembly 3 can move along the Y-axis direction, two Y-axis guide rails 112 extending along the Y-axis direction are arranged on the frame 1, and the connecting seat 81 is slidably connected with the two Y-axis guide rails 112. Specifically, the Y-axis guide rail 112 of the present embodiment is a guide rod, and the connection seat 81 is slidably connected to the guide rod through a linear bearing.

The X axle subassembly 2 of this embodiment includes X axle motor and X axle ball, and X axle motor stiff end sets up in frame 1, and the output of X axle motor links to each other with X axle ball's X axle screw, is equipped with Z axle subassembly 4 on two X axle nuts of X axle ball respectively, and X axle motor can drive X axle ball and drive two Z axle subassemblies 4 and move along the X axle direction simultaneously. In order to ensure that the X-axis assembly 2 can move along the X-axis direction, the frame 1 is provided with an X-axis guide rail 111 extending along the X-axis direction, and the Z-axis assembly 4 is slidably connected with the X-axis guide rail 111.

As shown in fig. 2, each Z-axis assembly 4 of the present embodiment includes a Z-axis motor 41 and a Z-axis ball screw 42, a fixed end of the Z-axis motor 41 is disposed on an X-axis nut, the Z-axis motor 41 is in transmission connection with the Z-axis ball screw 42, an air pump 5 is disposed on the Z-axis nut of the Z-axis ball screw 42, and the Z-axis motor 41 can drive the Z-axis ball screw 42 to drive the air pump 5 and the connecting member 7 to move along the Z-axis direction.

As shown in fig. 1, the multi-channel single-cell nucleic acid processing apparatus of this embodiment further includes a fixing base 113, the fixing base 113 is disposed on an X-axis nut of the X-axis ball screw, the Z-axis assembly 4 is fixedly disposed on the fixing base 113, in order to ensure that the air pump 5 moves along the Z-axis direction, a Z-axis guide rail 1131 extending along the Z-axis direction is disposed on the fixing base 113, and the air pump 5 is slidably connected to the Z-axis guide rail 1131. As shown in fig. 2, the Z-axis assembly 4 further includes a Z-axis conveying assembly 43, the Z-axis conveying assembly 43 includes a Z-axis driving wheel 431, a Z-axis driven wheel 432 and a Z-axis synchronous belt 433, the Z-axis driving wheel 431 is disposed at an output end of the Z-axis motor 41, the Z-axis motor 41 is located below the Z-axis driving wheel 431, the total height of the liquid transferring structure is reduced compared with the case that the Z-axis motor 41 is directly disposed above the Z-axis ball screw 42, the Z-axis driven wheel 432 is disposed on the Z-axis screw of the Z-axis ball screw 42, and the Z-axis synchronous belt 433 is respectively in transmission connection with the Z-axis driving wheel 431 and the Z-axis driven wheel 432.

In other embodiments, the Y-axis ball screw 32 of the Y-axis assembly 3 can be replaced by a Y-axis driving wheel, a Y-axis driven wheel and a Y-axis synchronous belt, the Y-axis driving wheel is disposed at the output end of the Y-axis motor 31, the Y-axis driven wheel can be rotatably disposed on the rack 1, the Y-axis synchronous belt is respectively connected with the Y-axis driving wheel and the Y-axis driven wheel in a transmission manner, the connecting seat 81 is fixedly connected with the Y-axis synchronous belt, the Y-axis motor 31 can drive the Y-axis driving wheel and the Y-axis synchronous belt to drive the connecting seat 81 to move along the Y-axis direction, and the Y-axis synchronous belt drives the carrier to move along the Y-axis direction through the forward rotation and the reverse rotation of the Y-axis motor 31. In other embodiments, the X-axis assembly 2, the Y-axis assembly 3, and the Z-axis assembly 4 may also be a structure of an electric push rod, an air cylinder, and a connecting rod, or other structures of linear motion, and are specifically configured according to actual needs.

As shown in fig. 1, the multi-channel single-cell nucleic acid processing apparatus of the embodiment further includes an X-axis limit switch (not shown), a Y-axis limit switch 101, and a Z-axis limit switch 102, where the X-axis limit switch is used to limit the maximum displacement of the X-axis assembly 2 driving the air pump 5 along the X-axis direction, the Y-axis limit switch 101 is used to limit the maximum displacement of the Y-axis assembly 3 driving the carrier along the Y-axis direction, the Z-axis limit switch 102 is used to limit the maximum displacement of the Z-axis assembly 4 driving the air pump 5 along the Z-axis direction, and the X-axis limit switch, the Y-axis limit switch 101, and the Z-axis limit switch 102 are all electrically connected to the controller.

It should be noted that the foregoing is only a preferred embodiment of the present invention and the technical principles applied. It will be understood by those skilled in the art that the present invention is not limited to the particular embodiments described herein, but is capable of various obvious changes, rearrangements and substitutions as will now become apparent to those skilled in the art without departing from the scope of the invention. Therefore, although the present invention has been described in greater detail with reference to the above embodiments, the present invention is not limited to the above embodiments, and may include other equivalent embodiments without departing from the scope of the present invention.

Claims (10)

1. A multi-channel single-cell nucleic acid processing instrument, comprising:

a frame (1);

the moving assembly is arranged on the rack (1) and comprises an X-axis assembly (2), a Y-axis assembly (3) and at least two Z-axis assemblies (4), and the X-axis assembly (2) can drive the at least two Z-axis assemblies (4) to move along the X-axis direction simultaneously;

the carrier is arranged at the output end of the Y-axis assembly (3), the carrier is used for placing a disposable suction tube (100) and a chip (200), and the Y-axis assembly (3) can drive the carrier to move along the Y-axis direction;

at least two air pumps (5), every air pump (5) all sets up one the output of Z axle subassembly (4) just is located the top of microscope carrier, every Z axle subassembly (4) all can drive one air pump (5) are along the motion of Z axle direction, every all fixed magnet (6) and one connecting piece (7) of being provided with on air pump (5), it can drive to move the subassembly air pump (5) drive magnet (6) with chip (200) laminating, every connecting piece (7) all can stretch into one in disposable straw (100) so that disposable straw (100) are fixed on connecting piece (7).

2. The multi-channel single-cell nucleic acid processing instrument according to claim 1, further comprising at least two blocking frames (91) disposed on the carrier, wherein the at least two blocking frames (91) are disposed corresponding to the at least two connectors (7), respectively, and a blocking groove (910) is disposed at an edge of the blocking frame (91), and the blocking groove (910) is used for blocking the disposable pipette (100) so as to separate the disposable pipette (100) from the connector (7).

3. The multi-channel single-cell nucleic acid processing apparatus as claimed in claim 1, wherein one pressure detecting member is provided in each of the air pumps (5).

4. The multi-channel single-cell nucleic acid processing instrument according to claim 1, wherein the Y-axis assembly (3) comprises a Y-axis motor (31) and a Y-axis ball screw (32), an output end of the Y-axis motor (31) is connected to a Y-axis screw of the Y-axis ball screw (32), and a Y-axis nut of the Y-axis ball screw (32) is connected to the stage.

5. The multi-channel single-cell nucleic acid processing instrument according to claim 1, wherein the Y-axis assembly (3) comprises a Y-axis motor (31), a Y-axis driving wheel, a Y-axis driven wheel and a Y-axis synchronous belt, the Y-axis driving wheel is arranged at the output end of the Y-axis motor (31), the Y-axis driven wheel is rotatably arranged on the rack (1), the Y-axis synchronous belt is in transmission connection with the Y-axis driving wheel and the Y-axis driven wheel respectively, the carrying platform is fixedly connected with the Y-axis synchronous belt, and the Y-axis motor (31) can drive the Y-axis driving wheel to drive the Y-axis synchronous belt and the carrying platform to move along the Y-axis direction.

6. The multi-channel single-cell nucleic acid processing instrument according to claim 1, wherein the X-axis assembly (2) comprises an X-axis motor and an X-axis ball screw, the Z-axis assembly (4) comprises a Z-axis motor (41) and a Z-axis ball screw (42), the fixed end of the X-axis motor is arranged on the rack (1), the output end of the X-axis motor is connected with the X-axis screw of the X-axis ball screw, the Z-axis motor (41) is arranged on the X-axis nut of the X-axis ball screw, the output end of the Z-axis motor (41) is in transmission connection with the Z-axis ball screw (42), and the air pump (5) is arranged on the Z-axis nut of the Z-axis ball screw (42).

7. The multi-channel single-cell nucleic acid processing instrument according to claim 1, wherein the stage comprises a connecting base (81) and at least two stage bodies (82) fixedly arranged on the connecting base (81), the connecting base (81) is arranged at an output end of the Y-axis assembly (3), the at least two stage bodies (82) are arranged in one-to-one correspondence with the at least two Z-axis assemblies (4), and each stage body (82) is provided with the disposable pipette (100) and the chip (200).

8. The multi-channel single-cell nucleic acid processing instrument according to claim 7, wherein one of the connecting seat (81) and the stage body (82) is provided with a guide (811), and the other is provided with a guide hole engaged with the guide (811).

9. The multi-channel single-cell nucleic acid processing instrument according to claim 7, wherein the connecting seat (81) and the carrier body (82) are connected by a bead screw, a mounting groove is provided on the connecting seat (81), the carrier body (82) is placed in the mounting groove, a fixed end of the bead screw is provided on the connecting seat (81) and located at an end of the connecting seat (81), and a movable end of the bead screw abuts against the carrier body (82) to position the carrier body (82) in the mounting groove.

10. The multi-channel single-cell nucleic acid processing instrument as claimed in claim 7, wherein the connecting seat (81) and the carrier body (82) are connected through an elastic pressing component (83), the elastic pressing component (83) is disposed on the connecting seat (81) and located at an end of the connecting seat (81), the elastic pressing component (83) comprises a spring sheet (831), and the spring sheet (831) can be pressed on the carrier body (82) to fix the carrier body (82) on the carrier body (82).

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