CN214694100U

CN214694100U - Biological enzyme purification and separation device

Info

Publication number: CN214694100U
Application number: CN202120158382.6U
Authority: CN
Inventors: 郑星; 殷科伦
Original assignee: Jintang Baixinwei Technology Co Ltd
Current assignee: Jintang Baixinwei Technology Co Ltd
Priority date: 2021-01-20
Filing date: 2021-01-20
Publication date: 2021-11-12
Anticipated expiration: 2031-01-20

Abstract

The utility model relates to a biological enzyme technical field provides a biological enzyme purification separator, including cooling tank, centrifugation jar and filtration jar, be connected with first conveyer pipe between cooling tank and the centrifugation jar, be connected with the second conveyer pipe between centrifugation jar and the filtration jar, be equipped with first delivery pump on the first conveyer pipe, be equipped with the second delivery pump on the second conveyer pipe, the cooling tank includes interior casing and shell body, forms the cooling chamber between interior casing and the shell body, first conveyer pipe runs through shell body and interior casing back and the inside intercommunication of interior casing in proper order. The cooling cavity is directly added in the cooling tank, so that the cooling medium is closer to the biological enzyme solution in the inner shell on the basis of not increasing the volume of the device as much as possible, thereby improving the cooling effect; meanwhile, the liquid level of the cooling liquid in the cooling cavity can be adjusted according to the liquid level of the biological enzyme solution in the inner shell, and the cooling effect is guaranteed to be in the best state.

Description

Biological enzyme purification and separation device

Technical Field

The utility model relates to a biological enzyme technical field particularly, relates to a biological enzyme purification separator.

Background

The purification and isolation of the biological enzyme refers to a process of treating an enzyme-containing raw material after cell disruption with an appropriate solvent under a certain condition to sufficiently dissolve the enzyme in an extract solution. Biological enzyme solution need carry out the cooling of a period of time before the purification separation to obtain better centrifugal effect, but present cooling arrangement mostly with centrifugal device phase separation, influenced the separation effect.

For this reason, patent publication No. CN211947013U discloses a biological enzyme purification separator, including the cooling tank, cooling jacket is installed to the lower part in the cooling tank, first conveyer pipe is installed to the cooling tank bottom, install first delivery pump on the first conveyer pipe, first conveyer pipe one end is connected with the cooling tank bottom, and the other end is connected with centrifuge tank upper portion, the centrifuge tank bottom is installed the second conveyer pipe, install the second delivery pump on the second conveyer pipe, second conveyer pipe one end with centrifuge tank bottom is connected, the other end with filter tank upper portion and be connected, centrifuge tank internally mounted has the filter screen. However, this purification and separation apparatus cools the solution by providing a cooling jacket outside the cooling tank, and thus has a poor cooling effect and an increased volume of the cooling tank.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a biological enzyme purification separator, it realizes improving the cooling effect on the basis that does not increase the cooling tank volume as far as possible through add the cooling chamber in cooling tank inside.

The embodiment of the utility model discloses a realize through following technical scheme:

a biological enzyme purification and separation device comprises a cooling tank, a centrifugal tank and a filtering tank, wherein a first conveying pipe is connected between the cooling tank and the centrifugal tank, a second conveying pipe is connected between the centrifugal tank and the filtering tank, a first conveying pump is arranged on the first conveying pipe, and a second conveying pump is arranged on the second conveying pipe;

the cooling tank comprises an inner shell and an outer shell, a cooling cavity is formed between the inner shell and the outer shell, and the first conveying pipe penetrates through the outer shell and the inner shell and then is communicated with the inside of the inner shell.

Optionally, the cooling device further comprises a liquid inlet pipe and a liquid outlet pipe which are respectively communicated with the cooling cavity, and valves are arranged on the liquid inlet pipe and the liquid outlet pipe.

Optionally, the inner shell is made of a low temperature resistant material, and the outer shell is made of a heat insulating material.

Optionally, the top of the cooling tank is provided with a liquid feeding pipe communicated with the inside of the inner shell, and the liquid feeding pipe is provided with a sealing cover capable of being opened and closed.

Optionally, a first transparent observation window is disposed on the side wall of the inner shell, and a second transparent observation window corresponding to the first transparent observation window is disposed on the side wall of the outer shell.

Furthermore, the height of the first transparent observation window is equal to the height of the inner cavity of the inner shell, and the height of the second transparent observation window is equal to the height of the first transparent observation window.

Furthermore, the outer wall of the second transparent observation window is provided with scale marks along the height direction.

Optionally, filter jar inside from last to being equipped with a plurality of filter screens down in proper order, the outer fringe of filter screen with filter jar's inner wall sliding fit, one side of filter screen runs through filter jar back-connection has the baffle, the inboard of baffle with filter jar's outer wall laminating, the baffle with filter between the jar detachably and connect.

Furthermore, a handle is arranged on the outer side of the baffle.

The utility model discloses technical scheme has following advantage and beneficial effect at least:

the utility model has reasonable design, and the cooling medium is more close to the biological enzyme solution in the inner shell on the basis of not increasing the volume of the cooling tank as much as possible by directly adding the cooling cavity in the cooling tank, thereby improving the cooling effect; meanwhile, the liquid level of the cooling liquid in the cooling cavity can be adjusted according to the liquid level of the biological enzyme solution in the inner shell, and the cooling effect is guaranteed to be in the best state.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

Fig. 1 is a schematic structural diagram of a biological purification and separation apparatus provided in an embodiment of the present invention;

fig. 2 is a schematic diagram of an internal structure of a cooling tank according to an embodiment of the present invention;

fig. 3 is an internal structure schematic diagram of a filter tank provided by the embodiment of the present invention.

Icon: 1-cooling tank, 101-inner shell, 102-outer shell, 103-cooling cavity, 2-centrifugal tank, 3-filtering tank, 4-first delivery pipe, 5-second delivery pipe, 6-first delivery pump, 7-second delivery pump, 8-liquid inlet pipe, 9-liquid outlet pipe, 10-liquid feeding pipe, 11-sealing cover, 12-first transparent observation window, 13-second transparent observation window, 14-filtering net, 15-baffle.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the present invention, it should be noted that, if the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship which is usually placed when the product of this application is used, the description is only for convenience of description and simplification, but the indication or suggestion that the device or element to be referred must have a specific orientation, be constructed in a specific orientation and be operated is not to be construed as limiting the present invention.

In the description of the present invention, it should be further noted that unless otherwise explicitly stated or limited, the terms "disposed," "mounted," "connected," and "connected" should be interpreted broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Examples

Referring to fig. 1 and 2, a biological enzyme purification and separation apparatus includes a cooling tank 1, a centrifugal tank 2, and a filtering tank 3, wherein a first delivery pipe 4 is connected between the cooling tank 1 and the centrifugal tank 2, a second delivery pipe 5 is connected between the centrifugal tank 2 and the filtering tank 3, the first delivery pipe 4 is provided with a first delivery pump 6, and the second delivery pipe 5 is provided with a second delivery pump 7; the above structure is the same as that of the prior art with the patent No. CN211947013U, and the steps of purifying and separating the bio-enzyme in this embodiment are the same as those of the prior art, and are not described herein.

In consideration of the fact that the cooling jacket is arranged outside the cooling tank 1 to cool the biological enzyme solution in the prior art, the volume of the device is increased, and the cooling tank 1 is partially uncovered by the cooling jacket, so that the cooling effect is poor. Therefore, the cooling tank 1 of the present embodiment includes an inner casing 101 and an outer casing 102, a cooling chamber 103 is formed between the inner casing 101 and the outer casing 102, and the first delivery pipe 4 penetrates the outer casing 102 and the inner casing 101 in this order and communicates with the inside of the inner casing 101. Simultaneously, this biological enzyme purification separator still includes feed liquor pipe 8 and drain pipe 9 with cooling chamber 103 intercommunication respectively, wherein feed liquor pipe 8 and drain pipe 9 and outside cooling medium source intercommunication, the upper portion space intercommunication of feed liquor pipe 8 and cooling chamber 103 of this embodiment, drain pipe 9 and cooling chamber 103's lower part space intercommunication, all be provided with the valve on feed liquor pipe 8 and the drain pipe 9.

Through the aforesaid setting, when needs cooling biological enzyme solution, open the valve of feed liquor pipe 8, close the valve of drain pipe 9, make the coolant (like water, liquid ammonia etc.) that outside coolant source provided pass through feed liquor pipe 8 and get into in the cooling chamber 103, treat that the liquid level of the coolant in the cooling chamber 103 reaches when with the liquid level height of the inside biological enzyme solution of interior casing 101, open the valve of drain pipe 9, and through the valve of adjusting feed liquor pipe 8 and drain pipe 9, make the flow of drain pipe 9 equal to the flow of feed liquor pipe 8, thereby realize that the coolant in the cooling chamber 103 still is in the circulation state under the unchangeable condition of liquid level height, guarantee that the cooling effect is in the optimum. Similarly, the flow rates of the liquid inlet pipe 8 and the liquid outlet pipe 9 can be adjusted by controlling the valves on the liquid inlet pipe 8 and the liquid outlet pipe 9, so that the liquid level height of the cooling medium in the cooling cavity 103 can be adjusted according to the change of the liquid level height of the biological enzyme solution in the inner shell 101. Therefore, in the present embodiment, the cooling cavity 103 is directly added to the cooling tank 1, so that the cooling medium is closer to the bio-enzyme solution in the inner shell 101 without increasing the volume of the device as much as possible, thereby improving the cooling effect.

Meanwhile, in order to further improve the cooling effect, the inner shell 101 of the present embodiment is made of a low temperature resistant material, so that the temperature of the bio-enzyme solution can be rapidly reduced, and the outer shell 102 is made of a heat insulating material, so that the external heat is prevented from being transferred into the cooling cavity 103, the temperature of the cooling medium is prevented from being affected, and the cooling effect is ensured.

In this embodiment, in order to add the bio-enzyme solution into the cooling tank 1, a filling tube 10 is disposed on the top of the cooling tank 1 and is communicated with the inside of the inner housing 101, a sealing cover 11 is disposed on the filling tube 10 and can be opened and closed, when the bio-enzyme solution is not added, the filling tube 10 can be sealed by the sealing cover 11, and thus, the external foreign matter is prevented from entering the inside of the inner housing 101.

In addition, considering that the amount of the biological enzyme solution in the cooling tank 1 needs to be observed in time in the actual purification and separation process, the biological enzyme solution can be added in time and the liquid level of the cooling medium in the cooling cavity 103 can be adjusted conveniently. Therefore, in the present embodiment, a first transparent observation window 12 is opened on a side wall of the inner housing 101, and a second transparent observation window 13 corresponding to the first transparent observation window 12 is opened on a side wall of the outer housing 102. The liquid level of the bio-enzyme solution inside the inner shell 101 and the liquid level of the cooling medium inside the cooling chamber 103 can be conveniently observed through the first transparent observation window 12 and the second transparent observation window 13. In addition, the height of the first transparent observation window 12 is equal to the height of the inner cavity of the inner case 101, and the height of the second transparent observation window 13 is equal to the height of the first transparent observation window 12, thereby maximizing the observable range. Meanwhile, in order to make the observation result more intuitive and accurate, the outer wall of the second transparent observation window 13 is provided with scale marks (not shown in the figure) along the height direction thereof.

Another convenience, filter tank 3 is inside from last to being equipped with a plurality of filter screens 14 down in proper order, consider above-mentioned prior art even also set up a plurality of filter screens 14 in order to realize the hierarchical filtration in filter tank 3. However, the filter screen 14 is often replaced by a new filter screen 14 after being used for a long time to ensure the filtering effect, but the filter screen 14 in the prior art is fixedly arranged in the filter tank 3, so that the later maintenance and replacement are inconvenient. Therefore, referring to fig. 3, in this embodiment, the outer edge of the filter screen 14 is in sliding fit with the inner wall of the filter tank 3, a baffle 15 is connected to one side of the filter screen 14 after penetrating through the filter tank 3, the inner side of the baffle 15 is attached to the outer wall of the filter tank 3, and the baffle 15 is detachably connected to the filter tank 3 through bolts. When filter screen 14 needs to be changed, only need lift off baffle 15 and filter the bolt of being connected usefulness between jar 3, can pull out filter screen 14 from filtering jar 3 through baffle 15 and change, change after the completion again through baffle 15 with filter screen 14 push into filter jar 3 in can, simple and convenient. In order to push and pull the baffle 15, a handle is arranged on the outer side of the baffle 15.

The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A biological enzyme purification and separation device comprises a cooling tank, a centrifugal tank and a filtering tank, wherein a first conveying pipe is connected between the cooling tank and the centrifugal tank, a second conveying pipe is connected between the centrifugal tank and the filtering tank, a first conveying pump is arranged on the first conveying pipe, a second conveying pump is arranged on the second conveying pipe,

2. The apparatus according to claim 1, further comprising a liquid inlet pipe and a liquid outlet pipe respectively connected to the cooling chamber, wherein the liquid inlet pipe and the liquid outlet pipe are provided with valves.

3. The apparatus for purifying and separating biological enzyme according to claim 1, wherein the inner casing is made of a low temperature resistant material, and the outer casing is made of a heat insulating material.

4. The apparatus according to claim 1, wherein a liquid feeding pipe is provided on the top of the cooling tank and communicates with the inside of the inner housing, and the liquid feeding pipe is provided with a sealing cover that can be opened and closed.

5. The apparatus according to claim 1, wherein a first transparent window is formed on a sidewall of the inner housing, and a second transparent window corresponding to the first transparent window is formed on a sidewall of the outer housing.

6. The apparatus of claim 5, wherein the first transparent window has a height equal to the height of the inner cavity of the inner housing, and the second transparent window has a height equal to the height of the first transparent window.

7. The apparatus for purifying and separating biological enzyme according to claim 6, wherein the outer wall of the second transparent observation window is provided with scale marks along the height direction thereof.

8. The biological enzyme purification and separation device according to claim 1, wherein a plurality of filter screens are sequentially arranged in the filter tank from top to bottom, the outer edges of the filter screens are in sliding fit with the inner wall of the filter tank, one side of each filter screen penetrates through the filter tank and is connected with a baffle, the inner side of each baffle is attached to the outer wall of the filter tank, and the baffle is detachably connected with the filter tank.

9. The apparatus for purifying and separating biological enzyme according to claim 8, wherein a handle is provided on the outside of the baffle.