CN214621949U - Sample applicator for microbial mass spectrum direct target plate coating method - Google Patents
Sample applicator for microbial mass spectrum direct target plate coating method Download PDFInfo
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- CN214621949U CN214621949U CN202120748979.6U CN202120748979U CN214621949U CN 214621949 U CN214621949 U CN 214621949U CN 202120748979 U CN202120748979 U CN 202120748979U CN 214621949 U CN214621949 U CN 214621949U
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Abstract
The utility model provides a microorganism mass spectrum directly scribbles target plate method sample spreader relates to the instrument technical field for microorganism mass spectrum sample preparation, install the smearing head in the at least one end of inoculation handle including inoculation handle and detachable, the end of smearing the head is equipped with paints the platform, paints the platform and is equipped with and paints the face, paints the face and is 30 ~65 with the contained angle of vertical direction or horizontal direction; the coating platform is disposable, and can be arranged into one of an umbrella-shaped coating platform, a box-shaped coating platform, a bullet-shaped coating platform and a hook-shaped coating platform according to different requirements; wherein, the coating platform is an umbrella-shaped coating platform provided with raised textures, and the side surface of the coating platform which is a bullet-shaped coating platform is provided with at least one triangular coating surface; the length of the top surface of the hook-shaped coating table is 2 mm-3mm, and the maximum width of the coating surfaces of other coating tables is not more than 2 mm; the utility model discloses can not influence when the coating and observe the inspection hole, paint a diameter and be less than the inspection hole diameter, easily observe the coating.
Description
Technical Field
The utility model relates to a tool technical field is used in preparation of microorganism mass spectrum sample, in particular to microorganism mass spectrum directly scribbles target plate method sample spreader.
Background
Currently, the most common method for preparing a microbial mass spectrum sample is a direct coating method, which needs to collect a strain to be detected and then uniformly coat the strain on a target plate. When the sample is not ideal to be smeared, the sample needs to be smeared again, because the detection aperture position is close to each other, no method is used for cleaning and wiping at present, and if smearing of the quality control hole position has problems, other 16 hole positions need to be smeared again.
The current manufacturer's recommended method is to coat with a 1uL inoculating loop, which has too large a surface area and a detection pore size of only 2-3 mm. Not only the quantity of the taken bacteria is not well controlled, but also the detection holes on the target plate are completely covered during coating, which is not beneficial to timely observation, and the limit is easily coated during the coating process.
SUMMERY OF THE UTILITY MODEL
The utility model provides a target plate method sample coating ware is directly scribbled to microorganism mass spectrum does not influence during the coating and observes the inspection hole, effectively avoids scribbling out the limit in the coating.
The embodiment of the utility model provides a target plate method sample coating ware is directly scribbled to microorganism mass spectrum, install the head of smearing in the at least one end of inoculation handle including inoculation handle and detachable, the end of smearing the head is equipped with paints the platform, paint the platform and be equipped with the face of smearing, the face of smearing is 30 ~65 with vertical direction or horizontal direction's contained angle.
In an alternative embodiment, the application surface is angled from 30 ° to 45 ° from vertical.
In an optional embodiment, the coating table is an umbrella-shaped coating table, the upper surface of the umbrella-shaped coating table is a coating surface, and an included angle between the upper surface of the umbrella-shaped coating table and the vertical direction is 30-65 degrees.
In an alternative embodiment, the application surface is provided with a raised texture.
In an optional embodiment, the smearing table is a box-shaped smearing table, the upper surface of the box-shaped smearing table is an arc-shaped smearing surface, and an included angle between the arc-shaped smearing surface and the vertical direction is 30-65 degrees.
In an optional embodiment, the application table is a bullet-shaped application table, and an included angle between the side surface of the bullet-shaped application table and the vertical direction is 15-30 °.
In an alternative embodiment, the side of the bullet-shaped application station is provided with at least one triangular application surface.
In an optional embodiment, the coating table is a hook-shaped coating table, the top surface of the hook-shaped coating table is a coating surface, and the included angle between the top surface of the hook-shaped coating table and the horizontal direction is 30-65 degrees.
In an alternative embodiment, the maximum width of the application surface is no greater than 2 mm.
In an alternative embodiment, the length of the top surface of the hook-shaped application table is 2mm to 3 mm.
The utility model has the advantages that: the sample applicator adopting the microbial mass spectrometry direct target plate coating method comprises a seed receiving handle and an applying head, wherein the tail end of the applying head is provided with an applying platform, the applying platform is provided with an applying surface, and the included angle between the applying surface and the vertical direction or the horizontal direction is 30-65 degrees, so that the seed receiving handle is convenient to hold by hand to form a certain inclination angle when the seed receiving handle is applied, the detection hole is convenient to observe, and the coating limit in coating is effectively avoided; the seed receiving handle can be held by a holding pen in a hand mode when the seed receiving handle is used, so that the use habit of an operator can be better met, the fitness is better, and the smearing range, the smearing force and the like can be more conveniently controlled; the inoculation handle is connected with the smearing head in a detachable mode, and the smearing head can be arranged to be disposable, so that only the smearing head needs to be replaced compared with a disposable inoculating ring, and cost is reduced.
Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and drawings.
In order to make the aforementioned and other objects, features and advantages of the present invention comprehensible, preferred embodiments accompanied with figures are described in detail below.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the technical solutions in the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a schematic structural diagram of an embodiment of the present invention;
fig. 2 is a schematic structural view of a seed receiving handle according to a first embodiment of the present invention;
fig. 3 is a schematic structural view of an applicator head according to a first embodiment of the present invention;
fig. 4 is a schematic structural diagram of a coating surface according to a first embodiment of the present invention;
fig. 5 is another schematic structural diagram of the application surface according to the first embodiment of the present invention;
fig. 6 is a schematic structural view of a second embodiment of the present invention;
fig. 7 is a schematic structural view of a coating surface according to a third embodiment of the present invention;
fig. 8 is a schematic structural view of a coating surface according to a third embodiment of the present invention;
fig. 9 is a schematic structural view of a coating surface according to a fourth embodiment of the present invention;
fig. 10 is a schematic structural view of a coating surface according to a fifth embodiment of the present invention;
fig. 11 is another schematic structural view of the application surface according to the fifth embodiment of the present invention;
fig. 12 is a schematic structural view of an applying surface according to a sixth embodiment of the present invention;
fig. 13 is another schematic structural view of an application surface according to a sixth embodiment of the present invention.
In the figure: 1-a seed-inoculating handle; 2, coating a head; 3, a coating platform; 4, coating a surface; 11-inoculation crown.
Detailed Description
To make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and obviously, the described embodiments are some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the use of the microbial mass spectrum, bacteria are required to be picked and then uniformly coated on round holes of a target plate of the microbial mass spectrum, the diameter of each round hole is about 2.5mm-3mm, the diameters of bacterial colonies are usually different from 0.2mm to 6mm, the shapes, sizes and properties of different bacteria are greatly different, a proper coating tool is required to pick and coat the bacteria, the uniform coating and the thickness directly influence the sampling speed and the sampling quality of the microbial mass spectrum, and certain influence is caused on an identification result. Due to the diversity and complexity of bacterial colonies, this part of the work can only be done manually at present, and therefore a suitable applicator tool has a great influence on the quality and efficiency of the work.
At present, manufacturers recommend using inoculating loops for coating, but the main role of inoculating loops is inoculating, which has the following disadvantages:
firstly, the quantity of the taken bacteria is not well controlled;
secondly, the detection holes on the target plate are completely covered in the coating process, so that the timely observation is not facilitated, and the limit is easily formed in the coating process;
the thickness of the coating is not easy to control;
fourthly, the handle of the inoculating loop is too soft, so that the smearing force is not easy to control;
the overall volume of the inoculating loop is too large, and the cost is too high.
Based on the above, the utility model provides a sample coater adopting a microorganism mass spectrum direct target plate coating method, which can effectively avoid coating a boundary in coating without influencing observation and detection holes during coating; and the fungus taking quantity is convenient to control, the smearing force and the smearing thickness are convenient to control, the structure is simple, the cost is low, and the operation is convenient.
To facilitate understanding of the present embodiment, the following examples are provided to describe a sample applicator for direct mass spectrometry of microorganisms disclosed in the present invention.
Example one
As shown in FIG. 1, the present embodiment provides a sample applicator for a microorganism mass spectrometry direct target plate method, which comprises a seed shaft 1 and an applicator head 2 detachably mounted on at least one end of the seed shaft 1; as shown in fig. 3, the tail end of the smearing head 2 is provided with a smearing table 3; as shown in fig. 4, the coating table 3 is provided with a coating surface 4, and an included angle between the coating surface 4 and the vertical direction or the horizontal direction is 30-65 °. Preferably, the angle between the smearing surface 4 and the vertical direction is 30-45 degrees.
Specifically, the inoculation handle 1 and the smearing head 2 are detachably connected, which means that the smearing head 2 can be clamped on the inoculation handle 1, can also be in threaded connection with the inoculation handle 1, and can also be detachably connected in other manners. The interior of the applicator head 2 of this embodiment is hollow and is provided with a fixed groove therein for detachable connection with the inoculation crown 11. The inoculation handle 1 is arranged to be reusable, and the smearing head 2 is arranged to be disposable. In this embodiment, the right end of the inoculation handle 1 is provided with a conical inoculation handle tip 11, the length of the inoculation handle 1 is 124.93mm, and the length of the inoculation handle tip 11 is 14.7 mm. The length of the smearing head 2 is 42.81mm, the smearing head 2 is sleeved on the inoculation handle head 11, and the diameter of the smearing head 2 is 5.03 mm.
As shown in fig. 3, an umbrella-shaped coating platform 3 with the diameter of 1.59 is arranged at the tail end of the coating head 2, the upper surface of the umbrella-shaped coating platform 3 is a coating surface 4, the included angle between the coating surface 4 and the vertical direction is 30-65 degrees, and the optimal included angle between the coating surface 4 and the vertical direction is 30-45 degrees. The smearing surface 4 is set to have a certain inclination angle, so that a worker can use a pen holding posture to hold the inoculation handle 1 when holding the inoculation handle 1, the inoculation handle 1 and the vertical direction form a certain inclination angle, the detection hole is convenient to observe during coating, and the operation is convenient.
Further, the maximum width of the application face 4 is not more than 2 mm.
Here, the maximum width of the smearing surface 4 is less than or equal to 2mm, namely less than the diameter of a sample hole of the target plate, thereby effectively avoiding smearing out a limit in smearing and being easy to control the smearing bacterial quantity.
Preferably, as shown in fig. 4 and 5, the application table 3 is an umbrella-shaped application table 3, the upper surface of the umbrella-shaped application table 3 is an application surface 4, and the included angle between (the tangent line of) the upper surface of the umbrella-shaped application table 3 and the vertical direction is 30-65 degrees, preferably 30-45 degrees.
Specifically, the umbrella-shaped (slope-shaped) coating surface 4 of the present embodiment is ergonomic, and the coating surface 4 is smooth and suitable for coating with ordinary bacteria.
The applicator head 2 of the present embodiment is made of a material having a certain adsorption ability to microorganisms, such as hard rubber or resin.
The maximum width of the smearing surface 4 is not more than 2mm, so that the area of the smearing surface 4 is smaller than that of the detection hole, and the bacterium taking amount is easy to control; the coating surface 4 is flat and easy to be coated more uniformly, and the thickness is easy to master; the smearing head 2 is made of materials such as hard rubber or resin and the like which have certain adsorbability on microorganisms, so that the bacterium taking efficiency can be improved; the mode of separating the inoculation handle 1 and the smearing head 2 is adopted, so that the volume of consumable materials is reduced.
Example two
As shown in fig. 6, unlike the first embodiment, the application table 3 of the present embodiment is an umbrella-shaped application table 3, and the umbrella-shaped application surface 4 is provided with raised textures.
Here, unlike the embodiment, the present embodiment provides several raised textures on the application surface 4, thereby facilitating the picking of colonies, easy grinding, and reduction of the thickness of applied bacteria.
EXAMPLE III
As shown in fig. 7 and 8, unlike the first embodiment, the application table 3 of the present embodiment is a box-shaped application table 3, the upper surface of the box-shaped application table 3 is an arc-shaped application surface 4, and an angle between (a tangent line of) the arc-shaped application surface 4 and the vertical direction is 30 ° to 65 °.
Specifically, the top end of the box body is arc-shaped, namely the box body is integral; the application table 3 of this example is 1.18mm long, 1.13mm wide and 0.5mm high. The coating surface 4 is an arc-shaped bulge, so that a small number of bacterial colonies can be conveniently picked from large bacterial colonies; after the target plate is spotted, one end (arc end) of the arc surface is used for coating.
The included angle between the arc-shaped coating surface 4 and the vertical direction is 30-65 degrees, and the arc-shaped coating surface is in accordance with human engineering and easy to operate.
Example four
As shown in fig. 9, unlike the first embodiment, the applying table 3 of the present embodiment is a bullet-shaped applying table 3, and an angle between a side surface (tangent line) of the bullet-shaped applying table 3 and the vertical direction is 15 ° to 30 °.
Here, the bullet-shaped application table 3 of the present embodiment also has the bullet-shaped application surface 4, and the side surface of the application surface 4 is an arc-shaped surface with a rounded tip. In the embodiment, the included angle of the tangent lines of the left side surface and the right side surface is 30-60 degrees, the periphery of the smearing surface 4 can be smeared, and the smearing surface can be rotated randomly in the smearing process until the smearing is uniform and the thickness is moderate.
EXAMPLE five
As shown in fig. 10 and 11, the bullet-shaped application table 3 of the present embodiment is provided with at least one triangular application surface 4 on the side surface, unlike the fourth embodiment.
Here, as shown in fig. 10, the bullet-shaped application table 3 has a triangular double-sided application surface 4 on its side surface, and the side of the side surface of this embodiment forms an angle of less than 30 ° with the vertical direction. The tip of this embodiment is smooth and rounded, and when applied on one side is found to be too thick, it can be rotated to the other side for continued application.
EXAMPLE six
As shown in fig. 12 and 13, unlike the first embodiment, the application table 3 of the present embodiment is a hook-shaped application table 3, the top surface of the hook-shaped application table 3 is an application surface 4, and the included angle between the top surface of the hook-shaped application table 3 and the horizontal direction is 30 ° to 65 °.
Further, the length of the top surface of the hook-shaped coating table 3 of the present embodiment is 2mm to 3 mm.
Here, the hook-shaped application table 3 of the present embodiment has an application surface 4 as a top surface and a length of 2.19 mm. The hook-shaped application station 3 of the present embodiment facilitates the detection of a fine application of the hole edge portion.
In summary, the above embodiments can select different application heads 2 to be used according to the size and growth status of the strain, different working environments, or different applications. The top end of the smearing head 2 of the embodiment is smooth and flat (except the embodiment), has toughness and good shape and detection hole site adhesiveness, and can enable different strains to be evenly coated on a target plate. The top end of the smearing head 2 in the second embodiment is a micro umbrella-shaped bulge, so that colonies can be picked more conveniently; the third embodiment, the fourth embodiment, the fifth embodiment and the sixth embodiment are respectively provided with a plurality of angular smearing surfaces 4, so that the control of the bacterium taking amount is facilitated in the smearing process; the strain with induration can be broken by small-amplitude pressing to ensure uniform smearing; the embodiment can be used for finishing the selection, the picking and the smearing at one time, so that different strains are uniformly coated on the target plate, the detection speed of the mass spectrum is improved, the service life of the mass spectrum is prolonged, and the consistency and the reliability of the result of the mass spectrum are ensured.
Finally, it should be noted that: the above-mentioned embodiments are only specific embodiments of the present invention, and are not intended to limit the technical solution of the present invention, and the protection scope of the present invention is not limited thereto, although the present invention is described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: those skilled in the art can still modify or easily conceive of changes in the technical solutions described in the foregoing embodiments or make equivalent substitutions for some technical features within the technical scope of the present disclosure; such modifications, changes or substitutions do not substantially depart from the spirit and scope of the embodiments of the present invention, and are intended to be included within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (10)
1. A sample applicator adopting a microbial mass spectrometry direct target plate coating method is characterized by comprising an inoculation handle and a detachable smearing head arranged at least one end of the inoculation handle, wherein a smearing table is arranged at the tail end of the smearing head, a smearing surface is arranged on the smearing table, and an included angle between the smearing surface and the vertical direction or the horizontal direction is 30-65 degrees.
2. The microbial mass spectrometry direct target coating plate method sample applicator of claim 1, wherein the angle between the application surface and the vertical direction is 30-45 °.
3. The microbial mass spectrometry direct target coating method sample applicator of claim 1, wherein the application table is an umbrella-shaped application table, the upper surface of the umbrella-shaped application table is an application surface, and the included angle between the upper surface of the umbrella-shaped application table and the vertical direction is 30-65 °.
4. The microbial mass spectrometry direct target coating sample applicator of claim 3, wherein the application surface is provided with raised texture.
5. The sample applicator for the direct microbial mass spectrometry target plate method according to claim 1, wherein the application platform is a box-shaped application platform, the upper surface of the box-shaped application platform is an arc-shaped application surface, and the included angle between the arc-shaped application surface and the vertical direction is 30-65 degrees.
6. The sample applicator for the direct microbial mass spectrometry target plate method according to claim 1, wherein the application table is a bullet-shaped application table, and the included angle between the side surface of the bullet-shaped application table and the vertical direction is 15-30 °.
7. The microbial mass spectrometry direct target coating sample applicator of claim 6, wherein the bullet-shaped coating platform is provided with at least one triangular coating surface on the side surface.
8. The microbial mass spectrometry direct target plate coating sample applicator of claim 1, wherein the application table is a hook-shaped application table, the top surface of the hook-shaped application table is an application surface, and the included angle between the top surface of the hook-shaped application table and the horizontal direction is 30-65 °.
9. The microbial mass spectrometry direct target plate method sample applicator of any one of claims 1-7, wherein the maximum width of the application surface is no greater than 2 mm.
10. The microbial mass spectrometry direct target coating sample applicator of claim 8, wherein the length of the top surface of the hook-shaped application station is 2mm to 3 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120748979.6U CN214621949U (en) | 2021-04-13 | 2021-04-13 | Sample applicator for microbial mass spectrum direct target plate coating method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120748979.6U CN214621949U (en) | 2021-04-13 | 2021-04-13 | Sample applicator for microbial mass spectrum direct target plate coating method |
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| Publication Number | Publication Date |
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| CN214621949U true CN214621949U (en) | 2021-11-05 |
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| CN202120748979.6U Active CN214621949U (en) | 2021-04-13 | 2021-04-13 | Sample applicator for microbial mass spectrum direct target plate coating method |
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- 2021-04-13 CN CN202120748979.6U patent/CN214621949U/en active Active
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