CN214612402U - Device convenient for filling cell culture solution or culture medium into culture bag - Google Patents
Device convenient for filling cell culture solution or culture medium into culture bag Download PDFInfo
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- CN214612402U CN214612402U CN202022710203.3U CN202022710203U CN214612402U CN 214612402 U CN214612402 U CN 214612402U CN 202022710203 U CN202022710203 U CN 202022710203U CN 214612402 U CN214612402 U CN 214612402U
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Abstract
The utility model discloses a device for conveniently packing cell culture solution or culture medium into culture bags, which belongs to the field of biological cell culture. The integral structure is E-shaped, and the main structure comprises a base, a middle plate, an upper plate, side plates and auxiliary components thereof. The height of the middle layer plate is adjustable, and the height of the liquid feeding pipe is adjustable. The design of adjustable height has made things convenient for the use of not equidimension culture bag. The not enough that prior art exists has been solved to whole technical scheme, promptly, when packing into the cell culture bag with cell suspension, can use multiple instrument, carries out the operation of multistep for the bagging-off process is loaded down with trivial details, and the consumptive material that the dress liquid used is disposable consumptive material mostly, can increase like this and consume, resource-wasting, and increase the risk of polluting. The device has the advantages of simple structure, convenient use, safe operation, repeated use, time saving, simplified steps, convenient loading of cell culture solution into the culture bag, capability of being placed into a sterile operation table and a biological safety cabinet for use, and obvious technical advantages.
Description
Technical Field
The utility model belongs to biological cell culture field, concretely relates to convenient device of going into cell culture liquid or culture medium toward culture bag.
Background
Cell culture refers to a method for simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions and the like) in vitro to enable the in vivo environment to survive, grow and reproduce and maintain main structures and functions, and the sterile environment, the proper temperature, the proper gas environment, a culture medium and the like are necessary environments and conditions for cell culture. Since cell culture itself is a large-scale clone of cells, and thus a large number of cells are obtained, in cell culture, expansion culture is required, for example, when expanding from culture flask culture to culture bag culture, cell culture bags are often used, and during the process of filling cell suspension into cell culture bags, the currently common method uses various tools and multi-step operation, which makes the bagging process cumbersome and increases the risk of contamination. Moreover, most consumables used for liquid filling are disposable consumables, so that the consumption is increased, and the resources are wasted.
Therefore, it is necessary to develop a technology of a cell culture bagging device which has a simple structure, is convenient to use, improves the operation safety, and can be repeatedly used.
Disclosure of Invention
In order to solve the problem, the utility model provides a novel cell sack filling device of convenient operation but also pollution abatement risk has realized that suspension sack filling easy operation is reliable, the step is simplified, resources are saved's purpose.
The utility model discloses a following technical scheme realizes:
a device for conveniently filling cell culture solution or culture medium into a culture bag comprises a liquid adding system positioned on the upper part, a culture bag fixing system positioned in the middle part and a base positioned at the bottom, wherein the liquid adding system, the culture bag fixing system and the base are connected together through a side vertical plate positioned on one side to be vertical, and the whole structure is in an E shape like a capital letter.
The liquid feeding system, the base and the side vertical plate are fixedly connected, and the culture bag fixing system and the side vertical plate are in sliding connection.
The liquid feeding system comprises an upper plate placed horizontally, a liquid feeding pipe, one side of the upper plate is fixedly connected with the upper end of a side plate, the upper plate is provided with a round hole which is communicated from top to bottom, the liquid feeding pipe vertically penetrates into the round hole of the upper plate, two bolt fixing blocks are fixedly arranged on two sides of the top surface of the upper plate close to the edge of the round hole respectively, two threaded holes which are communicated are formed in the two bolt fixing blocks, the axes of the two threaded holes are in line and pass through the circle center of the round hole of the upper plate, two first puller bolts are respectively penetrated in the two threaded holes, one ends of the two first puller bolts close to the round hole of the upper plate are respectively and rotatably connected with an arc clamping plate, and the two arc clamping plates are made to be far away from each other or close to the liquid feeding pipe which can be loosened or clamped to penetrate into the round hole of the upper plate through rotating the first puller bolts.
The culture bag fixing system comprises a middle layer plate, elastic sheet clamps and a displacement positioning part, wherein the middle part of one side of the middle layer plate is inwards sunken to form a slit, the middle layer plate is integrally in a concave shape, the displacement positioning part is formed by a cross beam and connecting rods which are vertically and fixedly connected with the two ends of the cross beam in a Contraband shape, the middle part of the cross beam is provided with a threaded hole and a second puller bolt penetrating into the threaded hole, the connecting rods at the two ends of the cross beam are vertically and fixedly connected with the two ends of the side edge opposite to the slit of the middle layer plate, the middle layer plate is horizontally arranged, a pair of elastic sheet clamps which are symmetrical by taking the vertical surface of the slit as a symmetrical surface are fixedly arranged at the inner part and the outer part of the two sides of the slit of the top surface of the middle layer plate respectively, the distance between the bottoms of the elastic sheet clamps and the connecting ends of the middle layer plate are separated by the slit, and the tops of the elastic sheet clamps are mutually extruded inwards by elasticity and are close to each other.
Specifically, the base is plate-shaped and horizontally placed, and one side edge of the base is vertically and fixedly connected with the lower end of the side vertical plate.
Specifically, the side vertical plate is a vertically arranged plate, and two vertical seams which are horizontally communicated and are arranged at the positions, close to two side edges, of the middle part of the side vertical plate are arranged.
The arc clamping plate and the first puller bolt are connected in a rotating mode, the connecting portion of the arc clamping plate and the first puller bolt is inwards recessed to form a cavity with a small opening and a large inner portion, and one end head portion of the first puller bolt connected with the arc clamping plate is expanded and the expanded head portion is located in the cavity of the arc clamping plate and can rotate freely and cannot be separated.
Specifically, the sliding connection of the middle layer plate and the side vertical plate is realized through the displacement positioning part of the culture bag fixing system, the specific structure is that two connecting rods of the displacement positioning part respectively penetrate through two vertical seams of the side vertical plate, and one end of a second puller bolt of the displacement positioning part is propped against the side surface of the side vertical plate.
Specifically, the main body part of the liquid feeding pipe is a hollow cylinder with openings at the upper end and the lower end, and the lower end part of the main body part of the liquid feeding pipe gradually becomes an open inverted cone to form a liquid leakage opening of the liquid feeding pipe.
The utility model discloses, a device of conveniently imbedding cell culture liquid or culture medium into culture bag, application method is as follows:
firstly, fixing the liquid adding pipe. The liquid feeding pipe vertically penetrates into a round hole of an upper plate, the upper half portion of the liquid feeding pipe is located on the upper plate, the lower half portion of the liquid feeding pipe is located below the upper plate, and the first tightening bolts on the two sides of the round hole are rotationally adjusted to enable the two arc-shaped clamping plates to be close to each other to achieve the purposes of clamping and fixing the liquid feeding pipe.
Secondly, fixing the culture bag and adjusting the height of the middle layer plate. The culture bag vertically penetrates through a slit of the middle layer plate from the lower part of the middle layer plate upwards and is squeezed into a spring clip fixedly arranged on the upper end face of the middle layer plate and is clamped by the spring clip to be suspended in the slit of the middle layer plate, the displacement positioning part and the side vertical plate are loosened through rotating and adjusting the second puller bolt to move up and down freely, the height of the middle layer plate is adjusted according to the length of the culture bag guide pipe, the upper end pipe orifice of the culture bag guide pipe can be sleeved and tightly sleeved into a liquid leakage opening of the liquid feeding pipe, and finally the second puller bolt is tightly abutted against the surface of the side vertical plate through rotating and adjusting the second puller bolt, so that the middle layer plate cannot move up and down, and the purpose of fixing the middle layer plate is achieved.
And thirdly, adding liquid. After the two steps of operations are finished, cell suspension is added into the liquid adding pipe through the pipe orifice at the upper end of the liquid adding pipe, and the cell suspension naturally flows downwards through the liquid leakage port and the culture bag conduit into the culture bag under the action of self gravity, so that the purpose of bagging cells is achieved.
And fourthly, arranging the devices. And after the cell bagging is completed through the three steps of operation, taking down the culture bag, taking down the liquid feeding pipe, cleaning the liquid feeding pipe, and collecting the device.
Compared with the prior art, the utility model has the advantages of as follows:
(1) the liquid feeding pipe is stable. The liquid feeding system is arranged, so that the liquid feeding pipe is stably clamped in the hole of the upper plate, and the liquid feeding pipe is prevented from shaking left and right due to the entering of liquid in the bagging process;
(2) the culture bag is stable. The culture bag fixing system is arranged, so that the culture bag is stably clamped and suspended in the slit of the middle layer plate, and the phenomenon that the culture bag shakes to fall off from the bottom of the liquid feeding pipe due to the entering of culture liquid in the bagging process is avoided;
(3) the height of the middle layer plate can be adjusted. The height of the middle layer plate can be adjusted according to the size of the cell culture bag, and meanwhile, the cell culture bag can be prevented from falling off or the top of the cell culture bag is prevented from being damaged due to the increase of culture solution and the increase of weight. In general, when the culture bag is filled with a culture medium or replenished with a culture medium in an early stage, the amount of the culture medium is small, the height of the middle plate is increased, and the flow of the culture medium into the culture bag is accelerated by the factor of gravity. Along with later stage liquid loading capacity or fluid infusion volume increase, weight increase in the culture bag, if still the height at the very beginning this moment, then can drop easily or damage the culture bag top because gravity involves, need down adjust suitable height with the height of intermediate lamella this moment suitably, make culture bag bottom contact the device base, rely on the base to provide the culture bag support.
(4) The cleaning and the replacement of accessories are convenient. After the cell culture bag liquid or the liquid supplement is finished, the first puller bolt of the upper plate is loosened, the liquid adding pipe is taken down, and the cell culture bag liquid or the liquid supplement can be used repeatedly after being cleaned and disinfected. The liquid feeding pipe can be replaced by a new one, and the sterile syringe outer cylinder can be used for replacing the liquid feeding pipe temporarily, so that the degree of freedom is high, and the use is convenient.
Drawings
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
Fig. 1 is a schematic view of the overall structure of the embodiment of the present invention.
Fig. 2 is a partially enlarged schematic view of a portion a in fig. 1.
Fig. 3 is a partially enlarged schematic view of a portion B in fig. 1.
FIG. 4 is a schematic view of FIG. 2 showing the culture bag removed and the observation angle changed.
FIG. 5 is a schematic structural view of a fixing system of a culture bag according to an embodiment of the present invention.
In the figure: 1-upper plate, 1-1-arc clamping plate, 1-2-bolt fixing block, 1-3-first puller bolt, 2-middle plate, 2-1-spring plate clamp, 2-2-displacement positioning part, 2-3-second puller bolt, 3-base, 4-side vertical plate, 5-liquid feeding pipe, 5-1-liquid leakage port, 6-culture bag and 6-1-culture bag guide pipe.
Detailed Description
The device for conveniently filling cell culture solution or culture medium into the culture bag comprises a liquid adding system positioned on the upper part, a culture bag fixing system positioned in the middle part and a base positioned at the bottom part, wherein the liquid adding system, the culture bag fixing system and the base are connected together through a side vertical plate positioned on one side to be vertical, and the whole structure is in an E shape like a capital letter. The liquid feeding system, the base and the side vertical plate are fixedly connected, and the culture bag fixing system and the side vertical plate are in sliding connection.
The liquid feeding system is by the upper plate that the level was placed, the filling tube constitutes, the perpendicular fixed connection in one side of upper plate and the upper end of side riser, the upper plate is opened there is a round hole that link up from top to bottom, the vertical round hole that penetrates the upper plate of filling tube, the both sides that the top surface of upper plate is close to the round hole edge are fixed respectively and are provided with a bolt fastening piece, all open the internal thread hole that has a link up on two bolt fastening pieces, the axis in two internal thread holes is in line and the centre of a circle through the upper plate round hole, wear a first puller bolt in two internal thread holes respectively, two first puller bolts are close to the one end of upper plate round hole and be swivelling joint respectively has an arc grip block, make two arc grip blocks keep away from each other or be close to can loosen or press from the filling tube that presss from both sides tightly and penetrate in the upper plate round hole through rotatory first puller bolt. The main body part of the liquid feeding pipe is a hollow cylinder with openings at the upper end and the lower end, and the lower end part of the liquid feeding pipe is gradually thinned into an open inverted cone to form a liquid leakage opening of the liquid feeding pipe. The rotary connection concrete structure of the arc-shaped clamping plate and the first puller bolt is that the connecting part of the arc-shaped clamping plate and the first puller bolt is inwards recessed to form a cavity with a small opening and a large inside, and the expanded head of one end of the first puller bolt connected with the arc-shaped clamping plate is positioned in the cavity of the arc-shaped clamping plate and can freely rotate and cannot be separated.
The culture bag fixing system comprises a middle layer plate, elastic sheet clamps and a displacement positioning part, wherein the middle part of one side of the middle layer plate is inwards sunken to form a slit, the middle layer plate is integrally in a concave shape, the displacement positioning part is formed by a cross beam and connecting rods which are vertically and fixedly connected with the two ends of the cross beam in a Contraband shape, the middle part of the cross beam is provided with a threaded hole and a second puller bolt penetrates into the threaded hole, the connecting rods at the two ends of the cross beam are vertically and fixedly connected with the two ends of the side edge opposite to the slit of the middle layer plate, the middle layer plate is horizontally arranged, a pair of elastic sheet clamps which are symmetrical by taking the vertical plane where the slit is located are respectively and fixedly arranged at the inner part and the outer part of the two sides of the slit of the top surface of the middle layer plate, the distance between the bottoms of the elastic sheet clamps and the connecting ends of the middle layer plate are mutually extruded inwards by elasticity and are tightly connected. The sliding connection of well plywood and side riser is realized through the displacement positioning part of culture bag fixed system, and concrete structure does, and two connecting rods of displacement positioning part pass two perps of side riser respectively, and the second puller bolt one end top of displacement positioning part is on the side of side riser, thereby can make the second puller bolt tightly push up on the side of side riser or loosen with the contact of the side of side riser through rotatory second puller bolt and make well plywood rigidity or can reciprocate.
The base is plate-shaped and horizontally placed, and one side edge of the base is vertically and fixedly connected with the lower end of the side vertical plate.
The side vertical plate is a vertically arranged plate, and two vertical seams which are horizontally communicated and are arranged at the middle part of the side vertical plate and are close to the two side edges are arranged.
The using method comprises the following steps:
firstly, fixing the liquid adding pipe. The liquid feeding pipe vertically penetrates into a round hole of an upper plate, the upper half portion of the liquid feeding pipe is located on the upper plate, the lower half portion of the liquid feeding pipe is located below the upper plate, and the first tightening bolts on the two sides of the round hole are rotationally adjusted to enable the two arc-shaped clamping plates to be close to each other to achieve the purposes of clamping and fixing the liquid feeding pipe.
Secondly, fixing the culture bag and adjusting the height of the middle layer plate. The culture bag vertically penetrates through a slit of the middle layer plate from the lower part of the middle layer plate upwards and is squeezed into a spring clip fixedly arranged on the upper end face of the middle layer plate and is clamped by the spring clip to be suspended in the slit of the middle layer plate, the displacement positioning part and the side vertical plate are loosened through rotating and adjusting the second puller bolt to move up and down freely, the height of the middle layer plate is adjusted according to the length of the culture bag guide pipe, the upper end pipe orifice of the culture bag guide pipe can be sleeved and tightly sleeved into a liquid leakage opening of the liquid feeding pipe, and finally the second puller bolt is tightly abutted against the surface of the side vertical plate through rotating and adjusting the second puller bolt, so that the middle layer plate cannot move up and down, and the purpose of fixing the middle layer plate is achieved.
And thirdly, adding liquid. After the two steps of operations are finished, cell suspension is added into the liquid adding pipe through the pipe orifice at the upper end of the liquid adding pipe, and the cell suspension naturally flows downwards through the liquid leakage port and the culture bag conduit into the culture bag under the action of self gravity, so that the purpose of bagging cells is achieved.
And fourthly, arranging the devices. And after the cell bagging is completed through the three steps of operation, taking down the culture bag, taking down the liquid feeding pipe, cleaning the liquid feeding pipe, and collecting the device.
Claims (9)
1. The utility model provides a make things convenient for go into device of cell culture liquid or culture medium in culture bag which characterized in that comprises the liquid feeding system that is located upper portion, the culture bag fixed system that is located the middle part, the base that is located the bottom, and liquid feeding system, culture bag fixed system, base link together by the side riser that is located one side and erects, and overall structure presents capital letter "E" font.
2. The apparatus as claimed in claim 1, wherein the feeding system, the base and the side standing plate are fixedly connected, and the culture bag fixing system and the side standing plate are slidably connected.
3. An apparatus for facilitating the filling of a culture bag with a cell culture solution or medium according to claim 1, it is characterized in that the liquid adding system consists of an upper plate and a liquid adding pipe which are horizontally arranged, one side of the upper plate is fixedly connected with the upper end of the side vertical plate, the upper plate is provided with a round hole which is communicated up and down, the liquid adding pipe vertically penetrates into the round hole of the upper plate, two bolt fixing blocks are respectively and fixedly arranged on the two sides of the top surface of the upper plate, which are close to the edge of the round hole, a through internal thread hole is respectively arranged on the two bolt fixing blocks, the axes of the two internal thread holes are in a straight line and pass through the circle center of the round hole of the upper plate, a first puller bolt is respectively penetrated in the two internal thread holes, one ends of the two first puller bolts, which are close to the round hole of the upper plate, are respectively and rotatably connected with an arc-shaped clamping plate, the two arc-shaped clamping plates are far away from or close to each other by rotating the first puller bolt, so that the liquid feeding pipe penetrating into the round hole of the upper plate can be loosened or clamped.
4. The apparatus as claimed in claim 1, wherein the culture bag fixing system comprises a middle plate, a spring clip, and a displacement positioning member, wherein a middle portion of one side of the middle plate is recessed inwardly to form a slit, the middle plate is integrally formed in a concave shape, the displacement positioning member comprises a cross beam and a connecting rod fixedly connected to two ends of the cross beam in a Contraband shape, the middle portion of the cross beam is provided with a threaded hole and a second tightening bolt is inserted into the threaded hole, the connecting rods at two ends of the cross beam are fixedly connected to two ends of the side opposite to the slit of the middle plate, the middle plate is horizontally disposed, a pair of spring clips symmetrical to the vertical plane of the slit are respectively fixed to two sides of the slit on the top surface of the middle plate, the bottom of each spring clip is spaced from the connecting end of the middle plate by a slit distance, the top parts of the spring clip are pressed inwards by elasticity and are close to each other.
5. The apparatus as claimed in claim 1, wherein the base is a plate and horizontally disposed, and one side of the base is vertically and fixedly connected to the lower end of the side vertical plate.
6. The apparatus as claimed in claim 1, wherein the side vertical plate is a vertically disposed plate, and two vertical slits are formed in the central portion of the side vertical plate near the two sides of the side vertical plate and extend horizontally therethrough.
7. The apparatus as claimed in claim 3, wherein the rotational connection between the arc-shaped holding plate and the first tightening bolt is configured such that the connection portion between the arc-shaped holding plate and the first tightening bolt is recessed inward to form a cavity with a small opening and a large interior, and the enlarged head portion of the first tightening bolt connected to the arc-shaped holding plate is located in the cavity of the arc-shaped holding plate and can rotate freely without coming off.
8. The device for conveniently filling cell culture solution or culture medium into the culture bag according to claim 4, wherein the sliding connection between the middle plate and the side plates is realized by a displacement positioning part of the culture bag fixing system, which has a specific structure that two connecting rods of the displacement positioning part respectively penetrate through two vertical slits of the side plates, and one end of a second tightening bolt of the displacement positioning part is pressed against the side surface of the side plates.
9. The apparatus for conveniently introducing a cell culture solution or a culture medium into a culture bag according to claim 3, wherein the main body of the filler tube is a hollow cylinder with openings at upper and lower ends, and the lower end of the filler tube is tapered into an open inverted cone to form a liquid leakage port of the filler tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022710203.3U CN214612402U (en) | 2020-11-20 | 2020-11-20 | Device convenient for filling cell culture solution or culture medium into culture bag |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022710203.3U CN214612402U (en) | 2020-11-20 | 2020-11-20 | Device convenient for filling cell culture solution or culture medium into culture bag |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214612402U true CN214612402U (en) | 2021-11-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022710203.3U Active CN214612402U (en) | 2020-11-20 | 2020-11-20 | Device convenient for filling cell culture solution or culture medium into culture bag |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214612402U (en) |
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2020
- 2020-11-20 CN CN202022710203.3U patent/CN214612402U/en active Active
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