CN214608229U

CN214608229U - Sealing device of functional PCR instrument

Info

Publication number: CN214608229U
Application number: CN202121009506.0U
Authority: CN
Inventors: 向洋; 周斌
Original assignee: Shanghai Saili Biotechnology Co ltd
Current assignee: Shanghai Saili Biotechnology Co ltd
Priority date: 2021-05-12
Filing date: 2021-05-12
Publication date: 2021-11-05
Anticipated expiration: 2031-05-12

Abstract

The utility model discloses a sealing device of a functional PCR instrument, which comprises a base, a feeding turntable which does intermittent motion is arranged on the base, a plurality of symmetrically arranged test tube positioning holes are arranged on the feeding turntable, a sealing mechanism matched with the feeding turntable is arranged on the base, the sealing mechanism comprises a U-shaped feeding track which is fixed on the base through a bracket, a first electric telescopic push rod is arranged in the U-shaped feeding track, the end part of the electric telescopic push rod is provided with a material pushing block, and the U-shaped feeding track is provided with a sealing cover feeding mechanism at the front end of the material pushing block; the end part of the U-shaped feeding track is provided with a vertically downward guide cover pipe which is butted with the U-shaped feeding track, and a vertically downward second electric telescopic push rod is fixed on the side wall of the guide cover pipe through a fixing frame. The utility model discloses can carry out the automation and seal, and need not the manual work and carry out manual sealing, avoid causing the pollution to the intraoral measuring object of test tube to have and seal efficient characteristics.

Description

Sealing device of functional PCR instrument

Technical Field

The utility model relates to a PCR detects technical field, specifically is a closing device of function PCR appearance.

Background

The real-time fluorescent quantitative PCR instrument consists of a fluorescent quantitative system and a computer and is used for monitoring the fluorescence in the circulating process. A computer connected to the real-time device collects the fluorescence data. The data is displayed in the form of a graph by developed real-time analysis software. Raw data are plotted as fluorescence intensity versus cycle number. Analysis can begin after raw data is collected. The software of the real-time device enables normalization of the collected data to compensate for differences in background fluorescence. The fluorescence quantitative PCR appearance of prior art does not all have the function of automatic sealing the test tube, need the experimenter to seal after hand again to put into fluorescence quantitative PCR appearance with the test tube, uses very troublesome to when artifical manual can seal the test tube mechanical energy, cause the pollution to the test tube mouth easily, thereby cause the pollution to the measuring thing in the test tube. Therefore, we improve this and propose a sealing device of a functional PCR instrument.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problem, the utility model provides a following technical scheme:

the utility model relates to a sealing device of function PCR appearance, the on-line screen storage device comprises a base, be equipped with the feeding carousel who is intermittent motion on the base, be equipped with the test tube locating hole that a plurality of symmetries set up on the feeding carousel, be equipped with on the base with feeding carousel complex sealing mechanism, sealing mechanism includes the U type feeding track of fixing on the base through the support, be equipped with first electronic flexible push rod in the U type feeding track, the tip of electronic flexible push rod is equipped with the ejector pad, the U type feeding track is equipped with the closing cap feeding mechanism at the front end that is located the ejector pad, closing cap feeding mechanism is including setting up the storage lid pipe on the U type feeding track, a plurality of closing caps have been stacked in the storage lid pipe, form the ejection of compact intermittent type that supplies a closing cap ejection of compact between the bottom of storage lid pipe and the U type feeding track; the end part of the U-shaped feeding track is provided with a vertical downward guide cover pipe which is butted with the U-shaped feeding track, a vertical downward second electric telescopic push rod is fixed on the side wall of the guide cover pipe through a fixing frame, and the end part of the second electric telescopic push rod is provided with a lower pressing block.

As an optimal technical scheme of the utility model, lead lid pipe and test tube locating hole butt joint.

As an optimal technical scheme of the utility model, the bottom of closing cap is equipped with the reducing post that the diameter diminishes gradually, closing cap and reducing post integrated into one piece just have medical rubber material to make.

As an optimized technical scheme of the utility model, the briquetting is located the center of closing cap pipe down, and with be equipped with the buffering intermittent type between the inner wall of closing cap pipe.

As a preferred technical scheme of the utility model, the support is the expansion bracket, the support is including setting up the locating rack on the base, be equipped with flexible chamber in the locating rack, flexible intracavity is equipped with along the expansion bracket that flexible chamber is flexible, the top and the U type pay-off track fixed connection of expansion bracket, be equipped with on the locating rack and be used for carrying out the manual positioning bolt fixed to the expansion bracket.

As an optimized technical scheme of the utility model, be equipped with height parameter mark on the lateral wall of expansion bracket.

The utility model has the advantages that: this kind of sealing device of function PCR appearance, can carry out automatic sealing to the mouth of pipe of the test tube that sends into in the PCR appearance and be equipped with the detection thing, and need not the manual work and carry out manual sealing, avoided causing the pollution to the detection thing in the test tube mouth, and have the characteristics that sealing efficiency is high, when sealing the test tube, at first put into the test tube locating hole on the pay-off carousel with the test tube that needs to seal, under the effect of pay-off carousel, send this test tube into under the pipe cap, then first electric telescopic push rod work, push the closing cap of storing up the bottom of pipe cap into the top of pipe cap, and thereby fall down in the pipe cap, and fall into on the mouth of pipe of test tube, then first electric telescopic push rod retracts, then the closing cap whereabouts in the pipe cap, carry out automatic feeding; the synchronous second electric telescopic rod is pressed downwards to drive the lower pressing block to press downwards, the sealing cover falling onto the pipe orifice of the test tube is extruded through the lower pressing block, the sealing cover is pressed into the pipe orifice of the test tube, so that the automatic sealing work of the test tube is realized, and then the second electric telescopic push rod retracts and resets to facilitate the next pressing and sealing work; the test tube after sealing is sent out under the action of the feeding turntable, and the test tube of the next station synchronously is sent into the sealing mechanism for sealing; make the utility model has the characteristics of seal effectual and seal efficiently.

Drawings

The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention. In the drawings:

FIG. 1 is a schematic structural view of a sealing device of a functional PCR instrument of the present invention;

FIG. 2 is a schematic structural diagram of a feeding turntable of a sealing device of a functional PCR instrument of the present invention;

FIG. 3 is a schematic structural diagram of a sealing cap of a sealing device of the functional PCR instrument of the present invention;

FIG. 4 is a schematic diagram of the intermittent discharge structure of the sealing device of the functional PCR instrument of the present invention.

In the figure: 1. a base; 2. a feeding turntable; 3. positioning holes of the test tubes; 4. a sealing mechanism; 5. a support; 6. a U-shaped feeding track; 7. an electric telescopic push rod; 8. a material pushing block; 9. a cover feeding mechanism; 10. a cover storage tube; 11. sealing the cover; 12. discharging intermittently; 13. a guide cover pipe; 14. a position fixing frame; 15. a second electric telescopic push rod; 16. pressing the block; 17. a variable diameter column; 18. a positioning frame; 19. a telescopic frame; 20. a manual positioning bolt; 21. and marking the height parameter.

Detailed Description

The preferred embodiments of the present invention will be described in conjunction with the accompanying drawings, and it will be understood that they are presented herein only to illustrate and explain the present invention, and not to limit the present invention.

Example (b): as shown in fig. 1, fig. 2, fig. 3 and fig. 4, the sealing device of the functional PCR instrument of the present invention comprises a base 1, the base 1 is provided with a feeding turntable 2 which does intermittent motion, the feeding turntable 2 is provided with a plurality of symmetrically arranged test tube positioning holes 3, the base 1 is provided with a sealing mechanism 4 matched with the feeding turntable 2, the sealing mechanism 4 comprises a U-shaped feeding track 6 fixed on the base 1 through a bracket 5, a first electric telescopic push rod 7 is arranged in the U-shaped feeding track 6, a material pushing block 8 is arranged at the end part of the electric telescopic push rod 7, the U-shaped feeding rail 6 is provided with a cover feeding mechanism 9 at the front end of the material pushing block 8, the cover feeding mechanism 9 comprises a cover storage pipe 10 arranged on the U-shaped feeding rail 6, a plurality of sealing covers 11 are stacked in the cover storage pipe 10, and a discharging interval 12 for discharging one sealing cover 11 is formed between the bottom end of the cover storage pipe 10 and the U-shaped feeding track 6; the end part of the U-shaped feeding track 6 is provided with a vertical downward guide cover pipe 13 which is butted with the U-shaped feeding track 6, the side wall of the guide cover pipe 13 is fixed with a second electric telescopic push rod 15 which is vertically downward through a position fixing frame 14, the end part of the second electric telescopic push rod 15 is provided with a lower pressing block 16 which can automatically seal the pipe orifice of a test pipe which is fed into the PCR instrument and is provided with a detection object without manually sealing, thereby avoiding the pollution to the detection object in the test pipe orifice and having the characteristic of high sealing efficiency, when the test pipe is sealed, firstly, the test pipe which needs to be sealed is put into a test pipe positioning hole 3 on a feeding turntable 2, under the effect of the feeding turntable 2, the test pipe is fed into the position under the guide cover pipe 13, then, the first electric telescopic push rod 7 works, a sealing cover 11 at the bottommost end of the storage cover pipe 10 is pushed into the position above the guide cover pipe 13, the cap guide pipe 13 falls down and falls onto the pipe orifice of the test pipe, then the first electric telescopic push rod 7 retracts, and the sealing cap 11 in the cap storage pipe 10 falls down for automatic feeding; the synchronous second electric telescopic rod is pressed downwards to drive the lower pressing block 16 to press downwards, the sealing cover 11 falling onto the pipe orifice of the test tube is extruded through the lower pressing block 16, the sealing cover 11 is pressed into the pipe orifice of the test tube, so that the automatic sealing work of the test tube is realized, and then the second electric telescopic push rod 157 retracts and resets to facilitate the next pressing and sealing work; the sealed test tube is sent out under the action of the feeding turntable 2, and the test tube of the next synchronous station is sent into the sealing mechanism 4 for sealing; make the utility model has the characteristics of seal effectual and seal efficiently.

Wherein, the guide cover pipe 13 is butted with the test tube positioning hole 3.

Wherein, the bottom of closing cap 11 is equipped with the reducing post 17 that the diameter diminishes gradually, closing cap 11 and reducing post 17 integrated into one piece just have medical rubber material to make, are convenient for impress closing cap 11 the mouth department of test tube mouth to be convenient for seal the test tube mouth.

Wherein, briquetting 16 is located the center of leading lid pipe 13, and is equipped with the buffering intermittent type between the inner wall with leading lid pipe 13 for 16 when pushing down the closing cap and supporting closing cap 11 to the test tube mouth down, can not take place the conflict effect with the port of test tube mouth.

Wherein, support 5 is the expansion bracket, support 5 is including setting up locating rack 18 on base 1, be equipped with flexible chamber in the locating rack 18, flexible intracavity is equipped with along the flexible expansion bracket 19 in flexible chamber, the top and the U type pay-off track 6 fixed connection of expansion bracket 19, be equipped with on the locating rack 18 and be used for carrying out the manual positioning bolt 20 fixed to expansion bracket 19, have simple structure's characteristics, convenient height-adjusting.

Wherein, be equipped with height parameter mark 21 on the lateral wall of expansion bracket 19, seal the test tube of co-altitude not, conveniently refer to when the height-adjusting of support 5.

The working principle is that when the test tube is sealed, the test tube to be sealed is firstly placed into a test tube positioning hole 3 on a feeding turntable 2, the test tube is fed under a guide cover pipe 13 under the action of the feeding turntable 2, a first electric telescopic push rod 7 works, a seal cover 11 at the bottommost end of a storage cover pipe 10 is pushed above the guide cover pipe 13, and then falls down in the guide cover pipe 13 and falls onto a pipe opening of the test tube, and then the first electric telescopic push rod 7 retracts, so that the seal cover 11 in the storage cover pipe 10 falls down to perform automatic feeding; the synchronous second electric telescopic rod is pressed downwards to drive the lower pressing block 16 to press downwards, the sealing cover 11 falling onto the pipe orifice of the test tube is extruded through the lower pressing block 16, the sealing cover 11 is pressed into the pipe orifice of the test tube, so that the automatic sealing work of the test tube is realized, and then the second electric telescopic push rod 157 retracts and resets to facilitate the next pressing and sealing work; the sealed test tube is sent out under the action of the feeding turntable 2, and the test tube of the next synchronous station is sent into the sealing mechanism 4 for sealing; make the utility model has the characteristics of seal effectual and seal efficiently.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing embodiments, or equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A sealing device of a functional PCR instrument comprises a base (1), wherein a feeding turntable (2) which does intermittent motion is arranged on the base (1), and the sealing device is characterized in that a plurality of symmetrically arranged test tube positioning holes (3) are arranged on the feeding turntable (2), a sealing mechanism (4) matched with the feeding turntable (2) is arranged on the base (1), the sealing mechanism (4) comprises a U-shaped feeding track (6) fixed on the base (1) through a support (5), a first electric telescopic push rod (7) is arranged in the U-shaped feeding track (6), a pushing block (8) is arranged at the end part of the electric telescopic push rod (7), a sealing cover feeding mechanism (9) is arranged at the front end of the pushing block (8) of the U-shaped feeding track (6), the sealing cover feeding mechanism (9) comprises a cover storage tube (10) arranged on the U-shaped feeding track (6), a plurality of sealing covers (11) are stacked in the cover storage pipe (10), and a discharging interval (12) for discharging one sealing cover (11) is formed between the bottom end of the cover storage pipe (10) and the U-shaped feeding track (6); the end part of the U-shaped feeding track (6) is provided with a vertical downward guide cover pipe (13) which is butted with the U-shaped feeding track (6), a vertical downward second electric telescopic push rod (15) is fixed on the side wall of the guide cover pipe (13) through a position fixing frame (14), and the end part of the second electric telescopic push rod (15) is provided with a lower pressing block (16).

2. The sealing device of a functional PCR instrument according to claim 1, wherein the cap guide tube (13) is butted against the tube positioning hole (3).

3. The sealing device of the functional PCR instrument according to claim 1, wherein the bottom of the sealing cap (11) is provided with a reducing column (17) with a gradually decreasing diameter, and the sealing cap (11) and the reducing column (17) are integrally formed and made of medical rubber.

4. The sealing device of the functional PCR instrument according to claim 1, wherein the pressing block (16) is located at the center of the cover guiding tube (13) and a buffering interval is arranged between the pressing block and the inner wall of the cover guiding tube (13).

5. The sealing device of the functional PCR instrument according to claim 1, wherein the support (5) is an expansion bracket, the support (5) comprises a positioning frame (18) arranged on the base (1), an expansion cavity is arranged in the positioning frame (18), an expansion bracket (19) which extends and retracts along the expansion cavity is arranged in the expansion cavity, the top of the expansion bracket (19) is fixedly connected with the U-shaped feeding track (6), and a manual positioning bolt (20) for fixing the expansion bracket (19) is arranged on the positioning frame (18).

6. The sealing device of the functional PCR instrument according to claim 5, wherein the side wall of the telescopic frame (19) is provided with a height parameter mark (21).