CN214529065U - Kit for NK cell culture - Google Patents

Abstract

The utility model discloses a kit for NK cell culture relates to biological apparatus technical field. The kit comprises a cylindrical shell and a reagent bottle, wherein a first heat-insulating layer is fixed on a bottom plate of the shell, and a second heat-insulating layer is fixed on an inner annular surface of a first annular wall of the shell; a cylinder is further fixed on the first heat-preservation layer, and a supporting plate and a limiting plate are arranged in the cylinder; the limiting plate is uniformly provided with a plurality of limiting holes, rubber rings are fixed in the limiting holes, and the supporting plate is provided with a plurality of rubber supporting blocks; the rubber supporting blocks correspond to the limiting holes one by one; the reagent bottle penetrates through the rubber ring, the bottom of the reagent bottle is placed on the corresponding rubber supporting block, and a top cover is further arranged on the shell. The kit can timely adjust the internal temperature according to the requirement of cell culture, monitors and displays the internal temperature in real time, and has the advantages of simple structure, simple and convenient use and good temperature keeping effect.

Description

Kit for NK cell culture

Technical Field

The utility model relates to a biological instrument technical field especially relates to a kit for NK cell culture.

Background

NK cells are natural killer cells (NK cells) which are important immune cells of the body and are related to tumor resistance, virus infection resistance and immune regulation. Currently, there are two culture modes for NK cell culture: a trophoblast cell (human tumor cell, namely human leukemia K562 cell) culture mode; first, trophoblast cells are obtained which have the property of not dividing and not proliferating but of maintaining metabolic activity. The cell is transformed by a gene engineering technology, and then irradiated by cobalt 60, the cell membrane surface stably expresses various cell factors, and the natural killer cells in the peripheral blood mononuclear cells are directionally activated and amplified under the synergistic action of the various cell factors. Pure factor cell culture mode: the corresponding cell factors are used for stimulating the mononuclear cells to induce the mononuclear cells to the direction of the NK cells, and the corresponding cell culture media are matched to enable the NK cells to proliferate in a large quantity. The reason why the pure factor cell culture technique is well known for its high safety is that all the cytokines used are originally present in the in vivo environment. The culture process is equivalent to simulating in vivo environment and promoting the activation and mass proliferation of NK cells. There are approximately 5% -15% NK cells in blood and we use leuca 15 and leuca 18 for activation.

Need use the kit among the culture process of current NK cell, generally put four and freeze and deposit the pipe, place coating agent, activator (white media 15 and white media 18 ═ 2:1), stabilizer, amplification agent (white media 2) respectively in freezing and depositing the pipe, the kit heat preservation device is the common apparatus in the biological assay, and it has special requirement to the temperature, in order to satisfy the requirement of cultivateing the NK cell, the utility model provides a kit for NK cell culture.

SUMMERY OF THE UTILITY MODEL

In order to solve the problem that exists, the utility model provides a kit for NK cell culture, this kit can in time adjust inside temperature according to cell culture's requirement to carry out real-time supervision and show its inside temperature, simple structure, it is simple and convenient to use, and the temperature keeps effectually.

In order to achieve the above purpose, the utility model adopts the following scheme,

a kit for NK cell culture comprises a cylindrical shell and a reagent bottle, wherein the shell comprises a bottom plate and a first annular wall, a first heat-insulating layer is fixed on the bottom plate of the shell, and a second heat-insulating layer is fixed on the inner annular surface of the first annular wall of the shell; a cylinder is further fixed on the first heat-preservation layer, a supporting plate and a limiting plate are arranged in the cylinder, and the limiting plate is positioned above the supporting plate; the limiting plate is uniformly provided with a plurality of limiting holes, rubber rings are fixed in the limiting holes, the supporting plate is provided with a plurality of rubber supporting blocks, and the rubber supporting blocks are provided with notches for accommodating the bottoms of the reagent bottles; the rubber supporting blocks correspond to the limiting holes one by one; the reagent bottle penetrates through the rubber ring, the bottom of the reagent bottle is placed on the corresponding rubber supporting block, and a top cover is further arranged on the shell.

Furthermore, the edge of the top cover extends downwards to form a second annular wall, an annular bulge is arranged on the inner annular surface of the second annular wall, a third heat-insulating layer is arranged between the top cover and the annular bulge, a pressing plate is arranged below the third heat-insulating layer, and the pressing plate is connected with the third heat-insulating layer through a plurality of springs; the inner annular surface of the second annular wall is provided with first threads, the outer annular surface at the upper end of the first annular wall is provided with second threads matched with the first threads, and the top cover is in threaded connection with the first annular wall of the shell through the second annular wall; the top cover and the third heat-insulating layer are provided with communicating holes corresponding in position, and the communicating holes are connected with hole plugs in a threaded mode.

Further, be equipped with detachable annular box between drum and the second heat preservation, just the annular box intussuseption is filled with water, still be equipped with double helix in the annular box, double helix's water inlet and delivery port are located annular box, just double helix's water inlet and delivery port are located the intercommunicating pore under.

Furthermore, a coolant pack is arranged between the cylinder and the second heat-insulating layer.

Furthermore, the inner wall of the cylinder is provided with a plurality of heat conducting rods.

Further, be equipped with the heating plate on the first heat preservation layer, layer board and limiting plate all are equipped with the round hole.

Furthermore, a groove is formed in the bottom plate, a power supply is arranged in the groove of the bottom plate, and the power supply is electrically connected with the heating plate.

Further, the lower surface of clamp plate is equipped with temperature sensor, be equipped with the LED display screen on the top cap, just temperature sensor and the equal electric connection power of LED display screen.

Further, a controller is further arranged in the groove of the bottom plate, and the controller is electrically connected with the heating plate, the power supply, the temperature sensor and the LED display screen respectively.

Furthermore, a sealing plate is arranged in the groove of the bottom plate, and the sealing plate seals the groove through a screw.

When the kit is used, the kit can be opened by screwing the top cover, when the required temperature is lower than the normal temperature, a coolant bag can be placed between the second heat preservation layer and the cylinder, and in addition, the heating plate can also be used for temperature regulation; when the required temperature is higher than the normal temperature, the annular box body can be placed between the second heat-insulating layer and the cylinder, the heating plate can be adopted to quickly heat up, and then the water inlet and the water outlet of the double-spiral pipe in the annular box body are connected with the external heat exchange equipment through the communication hole, so that the constant temperature state in the kit is ensured.

The utility model has the advantages that: 1) the limiting plate, the supporting plate and the pressing plate arranged in the kit can ensure that the reagent bottle cannot shake randomly in the kit, and the reagent bottle is prevented from toppling over; 2) the temperature sensor can measure the temperature in the kit and display the temperature on the LED display screen; 3) the second heat-insulating layer and the cylinder can be placed into a matched annular box body and matched with the heating plate to adjust and keep the temperature inside the kit to be higher than the normal temperature; 4) the second heat-insulating layer and the cylinder can also be placed in a coolant bag, and the second heat-insulating layer and the cylinder are matched with the heating plate to adjust and keep the temperature in the kit lower than the normal temperature; 5) the kit is also provided with a power supply, so that electric energy can be provided for the kit in a short time, and the short-distance transportation of the kit is facilitated.

Drawings

Fig. 1 is a schematic view of a sectional structure of the reagent kit in a first working state of the present invention.

Fig. 2 is a schematic view of a sectional structure of the reagent kit in a second working state of the present invention.

Fig. 3 is a schematic diagram of a supporting plate structure of the kit of the present invention.

Fig. 4 is a limiting plate structure schematic diagram of the kit of the present invention.

Detailed Description

Embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby.

It is to be noted that unless otherwise specified, technical or scientific terms used herein shall have the ordinary meaning as understood by those skilled in the art to which the present invention belongs.

In the description of the present application, it is to be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience of description and simplicity of description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be considered limiting.

Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.

In this application, unless expressly stated or limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can include, for example, fixed connections, removable connections, or integral parts; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In this application, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through intervening media. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

As shown in fig. 1-4, a kit for NK cell culture, comprising a cylindrical housing 1 and a reagent bottle 2, wherein the housing 1 comprises a bottom plate 1.1 and a first annular wall 1.2, a first heat-insulating layer 3.1 is fixed on the bottom plate 1.1 of the housing 1, and a second heat-insulating layer 3.2 is fixed on the inner annular surface of the first annular wall 1.2 of the housing 1; a cylinder 4 is further fixed on the first heat preservation layer 3.1, a supporting plate 5 and a limiting plate 6 are arranged in the cylinder 4, and the limiting plate 6 is positioned above the supporting plate 5; the reagent bottle rack is characterized in that a plurality of limiting holes 6.1 are uniformly formed in the limiting plate 6, rubber rings 6.2 are fixed in the limiting holes 6.1, a plurality of rubber supporting blocks 5.1 are arranged on the supporting plate 5, and notches for accommodating the bottoms of the reagent bottles 2 are formed in the rubber supporting blocks 5.1; the rubber supporting blocks 5.1 correspond to the limiting holes 6.1 one by one; the reagent bottle 2 penetrates through the rubber ring 6.2, the bottom of the reagent bottle is placed on the corresponding rubber supporting block 5.1, and the shell 1 is further provided with a top cover 7.

As shown in fig. 1 and 2, in order to enable the top cover 7 to be fixed on the housing 1, the edge of the top cover 7 extends downward to form a second annular wall 7.1, an annular bulge 7.2 is arranged on the inner annular surface of the second annular wall 7.1, a third insulating layer 3.3 is arranged between the top cover 7 and the annular bulge 7.2, a pressing plate 8 is arranged below the third insulating layer 3.3, and the pressing plate 8 and the third insulating layer 3.3 are connected through a plurality of springs 9; the inner annular surface of the second annular wall 7.1 is provided with first threads, the outer annular surface of the upper end of the first annular wall 1.2 is provided with second threads matched with the first threads, and the top cover 7 is in threaded connection with the first annular wall 1.2 of the shell 1 through the second annular wall 7.1; the top cover 7 and the third heat preservation layer 3.3 are provided with communicating holes 10 corresponding in position, the communicating holes 10 are connected with hole plugs 11 in an internal thread mode, and the communicating holes 10 can be used for enabling a camera to extend into the reagent bottle 2 inside the reagent kit to observe.

As shown in fig. 1 and 2, the inner wall of the cylinder 4 is provided with a plurality of heat conducting bars 4.1, which are beneficial to uniformly transferring heat.

As shown in fig. 1 and 2, be equipped with heating plate 15 on the first heat preservation 3.1, layer board 5 and limiting plate 6 all are equipped with the round hole, electric heating plate 15 can improve the inside temperature of kit, and round hole on layer board 5 and the limiting plate 6 is favorable to the heat upwards transmission of heating plate. In addition, a groove 1.3 is arranged in the bottom plate 1.1, a power supply 16 is arranged in the groove 1.3 of the bottom plate 1.1, and the power supply 16 is electrically connected with the heating plate 15. The power source 16 may be a battery to provide power to the reagent cartridge when the reagent cartridge is transferred.

As shown in fig. 1 and 2, a temperature sensor 17 is arranged on the lower surface of the pressing plate 8, an LED display screen 18 is arranged on the top cover 7, and the temperature sensor 17 and the LED display screen 18 are both electrically connected to the power supply 16. Still be equipped with controller 19 in the recess 1.3 of bottom plate 1.1, just controller 19 is connected with heating plate 15, power 16, temperature sensor 17 and LED display screen 18 electricity respectively. In this embodiment, the controller 19 mainly adopts a single chip microcomputer with the model of STC89C52, and in addition, the model of the temperature sensor 17 is DS18B 20; the 18-bit 4-bit common-anode LED nixie tube of the LED display screen; the temperature sensor 17 can continuously monitor the temperature in the kit, transmit signals to the single chip microcomputer in the controller for processing, and transmit the signals to the LED display screen by the single chip microcomputer to display the real-time temperature. In addition, the groove 1.3 of the bottom plate 1.1 is provided with a sealing plate 1.4, and the sealing plate 1.4 seals the groove 1.3 through a screw.

As shown in fig. 1, the first operating condition of kit, when demand temperature is higher than normal atmospheric temperature promptly be equipped with detachable annular box 12 between drum 4 and the second heat preservation 3.2, just annular box 12 intussuseption is filled with water, still be equipped with double helix 13 in the annular box 12, double helix 13's water inlet 13.1 and delivery port 13.2 are located annular box 12, just double helix 13's water inlet 13.1 and delivery port 13.2 are located the intercommunicating pore 10 under. The user can firstly adopt the heating plate 15 to rapidly heat up, and then the water inlet 13.1 and the water outlet 13.2 of the double-spiral pipe 13 in the annular box body 12 are externally connected with heat exchange equipment through the communicating hole 10, so that the constant temperature state in the kit is ensured.

As shown in fig. 2, when the second working state of the reagent box of the present invention, that is, the required temperature is lower than the normal temperature, a coolant bag 14 is provided between the cylinder 4 and the second insulating layer 3.2. The cold-storage agent package 14 is placed in a refrigerator for refrigeration, and is taken out and placed into the reagent kit when in use, and in addition, in order to prevent the temperature inside the reagent kit from being too low and in a lower constant temperature state, the heating plate 15 is controlled to heat, so that the temperature in the reagent kit is adjusted to meet the requirement.

Claims (10)

1. Kit for NK cell culture, comprising a cylindrical housing (1) and a reagent bottle (2), said housing (1) comprising a bottom plate (1.1) and a first annular wall (1.2), characterized in that: a first heat-insulating layer (3.1) is fixed on a bottom plate (1.1) of the shell (1), and a second heat-insulating layer (3.2) is fixed on the inner annular surface of a first annular wall (1.2) of the shell (1); a cylinder (4) is further fixed on the first heat-insulating layer (3.1), a supporting plate (5) and a limiting plate (6) are arranged in the cylinder (4), and the limiting plate (6) is positioned above the supporting plate (5); the reagent bottle rack is characterized in that a plurality of limiting holes (6.1) are uniformly formed in the limiting plate (6), rubber rings (6.2) are fixed in the limiting holes (6.1), a plurality of rubber supporting blocks (5.1) are arranged on the supporting plate (5), and notches for accommodating the bottoms of the reagent bottles (2) are formed in the rubber supporting blocks (5.1); the rubber supporting blocks (5.1) correspond to the limiting holes (6.1) one by one; the reagent bottle (2) penetrates through the rubber ring (6.2), and the bottom of the reagent bottle is placed on the corresponding rubber supporting block (5.1); the shell (1) is also provided with a top cover (7).

2. The kit for NK cell culture according to claim 1, characterized in that: the edge of the top cover (7) extends downwards to form a second annular wall (7.1), an annular bulge (7.2) is arranged on the inner annular surface of the second annular wall (7.1), a third heat-insulating layer (3.3) is arranged between the top cover (7) and the annular bulge (7.2), a pressing plate (8) is arranged below the third heat-insulating layer (3.3), and the pressing plate (8) is connected with the third heat-insulating layer (3.3) through a plurality of springs (9); the inner annular surface of the second annular wall (7.1) is provided with first threads, the outer annular surface of the upper end of the first annular wall (1.2) is provided with second threads matched with the first threads, and the top cover (7) is in threaded connection with the first annular wall (1.2) of the shell (1) through the second annular wall (7.1); the top cover (7) and the third heat-insulating layer (3.3) are provided with communicating holes (10) corresponding in position, and the communicating holes (10) are internally threaded with hole plugs (11).

3. The kit for NK cell culture according to claim 2, characterized in that: be equipped with detachable annular box (12) between drum (4) and second heat preservation (3.2), just annular box (12) intussuseption is filled with water, still be equipped with double helix (13) in annular box (12), water inlet (13.1) and delivery port (13.2) of double helix (13) are located annular box (12), just water inlet (13.1) and delivery port (13.2) of double helix (13) are located intercommunicating pore (10) under.

4. The kit for NK cell culture according to claim 2, characterized in that: a coolant pack (14) is arranged between the cylinder (4) and the second heat-insulating layer (3.2).

5. Kit for NK cell culture according to claim 3 or 4, characterized in that: the inner wall of the cylinder (4) is provided with a plurality of heat conducting rods (4.1).

6. The kit for NK cell culture according to claim 2, characterized in that: be equipped with heating plate (15) on first heat preservation (3.1), layer board (5) and limiting plate (6) all are equipped with the round hole.

7. The kit for NK cell culture according to claim 6, characterized in that: be equipped with recess (1.3) in bottom plate (1.1), be equipped with power (16) in recess (1.3) of bottom plate (1.1), heating plate (15) are connected to power (16) electricity.

8. Kit for NK cell culture according to claim 7, characterized in that: the lower surface of clamp plate (8) is equipped with temperature sensor (17), be equipped with LED display screen (18) on top cap (7), just temperature sensor (17) and the equal electric connection power supply (16) of LED display screen (18).

9. Kit for NK cell culture according to claim 8, characterized in that: still be equipped with controller (19) in recess (1.3) of bottom plate (1.1), just controller (19) are connected with heating plate (15), power (16), temperature sensor (17) and LED display screen (18) electricity respectively.

10. Kit for NK cell culture according to claim 8, characterized in that: the groove (1.3) of the bottom plate (1.1) is provided with a sealing plate (1.4), and the sealing plate (1.4) seals the groove (1.3) through a screw.

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