CN214486940U - micro-Raman spectrum micro-fluidic chip - Google Patents
micro-Raman spectrum micro-fluidic chip Download PDFInfo
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- CN214486940U CN214486940U CN202120480291.4U CN202120480291U CN214486940U CN 214486940 U CN214486940 U CN 214486940U CN 202120480291 U CN202120480291 U CN 202120480291U CN 214486940 U CN214486940 U CN 214486940U
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Abstract
The utility model provides a micro-raman spectroscopy micro-fluidic chip, including the polydimethylsiloxane substrate and be formed at liquid flow channel in the polydimethylsiloxane substrate, liquid flow channel includes following structure: the device comprises a sample inlet, an SERS reagent inlet, an aggregating agent inlet, a microfluidic reaction channel, a detection liquid pool and a waste liquid outlet; the sample inlet, the SERS reagent inlet, the aggregating agent inlet, the microfluidic reaction channel, the detection liquid pool and the waste liquid outlet are communicated with each other, wherein the tail ends of the sample inlet, the SERS reagent inlet, the aggregating agent inlet and the waste liquid outlet are positioned on the outer surface of the polydimethylsiloxane substrate and are communicated with the outside; the microfluidic reaction channel and the detection liquid pool are sealed inside the polydimethylsiloxane substrate. The micro-fluidic chip of the utility model is fully mixed, and the Raman enhanced signal can be obviously improved; the SERS reagent is not needed to be bonded in the channel, the use is simple and convenient, the cost is saved, and the popularization is easy.
Description
Technical Field
The utility model belongs to the technical field of analytical chemistry, especially, relate to a micro-fluidic chip that can be used to laser confocal micro-Raman spectrometer detects.
Background
The micro-fluidic chip is a technology for accurately controlling and controlling micro-scale fluid, can integrate basic operation units of sample preparation, reaction, separation, detection and the like in biological, chemical and medical analysis processes on a micro-scale chip, automatically completes the whole analysis process, and has huge application potential in the fields of biology, chemistry, medicine and the like.
The micro-fluidic chip adopts micro-electromechanical processing technology similar to semiconductors to construct a micro-channel system on the chip, the experiment and analysis processes are transferred to a chip structure consisting of a path and a small liquid-phase chamber which are mutually connected, and a micro-mechanical pump is adopted after a biological sample and reaction liquid are loaded. The buffer solution in the chip is driven to flow by methods such as an electro-hydraulic pump, electroosmotic flow and the like to form a micro flow path, and one or more continuous reactions are carried out on the chip. The microfluidic chip has the advantages of controllable liquid flow, few consumed samples and reagents, extremely high analysis speed and the like, and can simultaneously analyze different samples in several minutes or even shorter time. Therefore, the micro-fluidic chip has wide application prospect in the field of analysis and detection.
The Raman spectrum, especially the laser confocal Raman spectrum which is the mainstream at present, can provide molecular fingerprint information, the signal of the Raman spectrum can not be interfered by water, and the Raman spectrum can carry out rapid qualitative and nondestructive analysis on a sample and has great potential in a microfluidic analysis and detection technology. However, the intrinsic raman signals of the samples are weak, and the application needs to be performed by some methodsIt is enhanced. The Surface Enhanced Raman Scattering (SERS) is a commonly used enhancement method, and an active substrate is prepared by adopting metal nano materials such as Au, Ag and the like, so that the detection sensitivity can be improved by 103～107And (4) doubling. In the detection process, metal sol (SERS reagent) is often mixed with a sample to be detected in advance, a proper amount of aggregating agent is added at the same time, and after the metal sol, the SERS reagent and the sample are fully mixed, microscopic Raman spectrum detection is carried out. The commonly used aggregating agent is sodium chloride solution, which has the function of promoting silver sol nanoparticles to agglomerate in the solution to form hot spots, and can realize SERS signal enhancement (see Guo Xiaoying, Qiui, Zhang Chengjie, Yangdanting, Thangolan, Xuda Lun, Lou Qiaming, Yangwen pigeon, Huqijie. surface enhanced Raman spectroscopy for fast quantitative analysis of histamine in fish meat [ J]Spectroscopy and spectral analysis, 2019,39(08): 2561-. However, in the actual detection process, the problems of insufficient and uneven mixing of the sample to be detected, the SERS reagent and the aggregating agent are often encountered, so that the Raman enhancement signal is weak or not obvious, and the detection result is influenced.
SUMMERY OF THE UTILITY MODEL
In view of the above shortcoming of the prior art, the utility model aims to provide a micro-raman spectroscopy micro-fluidic chip for solve among the prior art to be measured sample and SERS reagent and the gathering agent insufficient mixing, inhomogeneous difficult problem.
In order to achieve the above objects and other related objects, the present invention provides a micro-raman spectroscopy microfluidic chip, including a polydimethylsiloxane substrate, and a liquid flow channel formed in the polydimethylsiloxane substrate, wherein the liquid flow channel includes the following structure: the device comprises a sample inlet, an SERS reagent inlet, an aggregating agent inlet, a microfluidic reaction channel, a detection liquid pool and a waste liquid outlet; the sample inlet, the SERS reagent inlet, the aggregating agent inlet, the microfluidic reaction channel, the detection liquid pool and the waste liquid outlet are communicated with each other, wherein the tail ends of the sample inlet, the SERS reagent inlet, the aggregating agent inlet and the waste liquid outlet are positioned on the outer surface of the polydimethylsiloxane substrate and are communicated with the outside; the microfluidic reaction channel and the detection liquid pool are sealed in the polydimethylsiloxane substrate; the microfluidic reaction channel is a continuous semicircular tubular structure, and the inner diameter of the microfluidic reaction channel is 100-200 mu m; the detection liquid pool is cylindrical, and the radius of the bottom surface is 2.5-3 mm. Preferably, the micro-Raman spectrum micro-fluidic chip is in a cuboid shape, the length is 5-6cm, the width is 2-3cm, and the height is 0.5-1 cm.
As described above, the micro-raman spectroscopy micro-fluidic chip of the present invention, by designing a plurality of continuous semi-circular tubular structures, makes the sample mix with higher speed through the collision force in the channel structure, significantly increases the example of mixing, and the violent vortex motion generated promotes the rapid mixing of the fluid, and therefore, has the following beneficial effects: mixing is sufficient, and Raman enhanced signals are obviously improved; the SERS reagent is not needed to be bonded in the channel, the use is simple and convenient, the cost is saved, and the popularization is easy.
Drawings
Fig. 1 shows the overall structure of the micro-raman spectroscopy microfluidic chip of the present invention.
Fig. 2 shows a comparison graph of the mixing effect and other mixing effects of the micro-raman spectroscopy micro-fluidic chip of the present invention.
Element number description: 1. the device comprises a sample inlet, 2, an SERS reagent inlet, 3, a aggregating agent inlet, 4, a microfluidic reaction channel, 5, a detection liquid pool, 6 and a waste liquid outlet.
Detailed Description
The following description is provided for illustrative purposes, and other advantages and features of the present invention will become apparent to those skilled in the art from the following detailed description.
It should be understood that the structure, ratio, size and the like shown in the drawings attached to the present specification are only used for matching with the content disclosed in the specification, so as to be known and read by those skilled in the art, and are not used for limiting the limit conditions that the present invention can be implemented, so that the present invention has no technical essential meaning, and any structure modification, ratio relationship change or size adjustment should still fall within the scope that the technical content disclosed in the present invention can cover without affecting the function that the present invention can produce and the purpose that the present invention can achieve. Meanwhile, the terms such as "upper", "lower", "left", "right", "middle" and "one" used in the present specification are for convenience of description, and are not intended to limit the scope of the present invention, and changes or adjustments of the relative relationship thereof may be made without substantial technical changes, and the present invention is also regarded as the scope of the present invention.
Referring to fig. 1, the present invention provides a micro-raman spectroscopy microfluidic chip, including a polydimethylsiloxane substrate and a liquid flow channel formed in the polydimethylsiloxane substrate, wherein the liquid flow channel includes the following structure: the device comprises a sample inlet, an SERS reagent inlet, an aggregating agent inlet, a microfluidic reaction channel, a detection liquid pool and a waste liquid outlet; the sample inlet, the SERS reagent inlet, the aggregating agent inlet, the microfluidic reaction channel, the detection liquid pool and the waste liquid outlet are communicated with each other, wherein the tail ends of the sample inlet, the SERS reagent inlet, the aggregating agent inlet and the waste liquid outlet are positioned on the outer surface of the polydimethylsiloxane substrate and are communicated with the outside; the microfluidic reaction channel and the detection liquid pool are sealed in the polydimethylsiloxane substrate; the microfluidic reaction channel is a continuous semicircular tubular structure, and the inner diameter of the microfluidic reaction channel is 100-200 mu m; the detection liquid pool is cylindrical, and the radius of the bottom surface is 2.5-3 mm. The micro-Raman spectrum micro-fluidic chip is in a cuboid shape, the length is 5-6cm, the width is 2-3cm, and the height is 0.5-1 cm. The SERS reagent is a surface enhanced Raman reagent, belongs to conventional reagents in the field, and can be commercially available or prepared by conventional technical means, and the silver sol is a common SERS reagent in the field, and a typical preparation method comprises the following steps: (1) preparation of silver sol according to the literature (Yan is handsome, Liyongyu, penquen, Liuyaultra, Korean east sea) and based on the internal standard of substrate]Spectroscopy and Spectroscopy 2021,41(02):546--3mol·L-1Heating the silver nitrate solution to boiling, stirring the silver nitrate solution at a high speed by using a magnetic stirrer, dropwise adding a 1% sodium citrate solution, keeping the volume ratio of the sodium citrate to the silver nitrate solution at 1: 40, heating and stirring for 1h, and naturally cooling to room temperature after the reaction is finished. Placing the silver sol in a table centrifuge at 3000 r.min-1Speed of rotationCentrifuging for 30min, and removing supernatant to obtain concentrated silver sol, namely the surface enhanced Raman reagent. (2) Another common Raman reagent synthesis method can be used for reference literature synthesis (Huang Yi Wei, Lin Jia, Xitang, Wei Bao Ying, Li Jianfeng, surface enhanced Raman spectroscopy rapid detection of phthalate in textile [ J]Spectroscopy and spectral analysis 2020,40(03):760 and 764): boiling 200mL of chloroauric acid solution with the concentration of 0.01 Wt%, quickly adding 1.5mL of sodium citrate solution (with the concentration of 1 Wt%) at one time, changing the solution into reddish brown after about 3min, keeping boiling for 30min, and cooling to obtain the surface enhanced Raman reagent. The aggregating agent is selected from sodium chloride solution. The utility model provides a micro-raman spectroscopy micro-fluidic chip's sample entry, SERS reagent entry, aggregating agent entry and waste liquid outlet's end is connected with the sample injector through PEEK back taper joint, and the sample injector commonly used can be selected from ten passageway injection pumps in lange laboratory.
For verifying the utility model provides a micro-fluidic chip's mixed effect, we have studied the mixed mode of several kinds of differences, contrast its raman spectrum signal power. The SERS reagent is silver sol purchased from general nanotechnology company of Xiamen, the sample to be detected is 10% (wt/wt) rhodamine B, and the aggregating agent is 0.2mol/L sodium chloride solution. Respectively mixing a sample to be detected, the SERS reagent and the aggregating agent according to the volume ratio of 1:1:0.5, wherein the mixing mode is selected from (a) mixing of the micro-fluidic chip provided by the utility model, (b) mixing of ultrasonic vibration, (c) mixing of vortex vibration, (d) mixing of violent manual mixing, and (e) natural standing after mixing. The instrument model is confocal laser microscopy raman spectroscopy Renishaw inVia, and the raman spectrogram that different mixed mode corresponds refers to description attached drawing 2, can see the utility model provides a raman signal is strongest behind the micro-fluidic chip mixing, explains to mix fully, and the effect is best.
The above embodiments are merely illustrative of the principles and effects of the present invention, and are not to be construed as limiting the invention. Modifications and variations can be made to the above-described embodiments by those skilled in the art without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which may be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.
Claims (3)
1. A micro-Raman spectrum microfluidic chip is characterized by comprising a polydimethylsiloxane substrate and a liquid flow channel formed in the polydimethylsiloxane substrate, wherein the liquid flow channel comprises the following structures: the device comprises a sample inlet, an SERS reagent inlet, an aggregating agent inlet, a microfluidic reaction channel, a detection liquid pool and a waste liquid outlet; the sample inlet, the SERS reagent inlet, the aggregating agent inlet, the microfluidic reaction channel, the detection liquid pool and the waste liquid outlet are communicated with each other, wherein the tail ends of the sample inlet, the SERS reagent inlet, the aggregating agent inlet and the waste liquid outlet are positioned on the outer surface of the polydimethylsiloxane substrate and are communicated with the outside; the microfluidic reaction channel and the detection liquid pool are sealed in the polydimethylsiloxane substrate; the microfluidic reaction channel is a continuous semicircular tubular structure, and the inner diameter of the microfluidic reaction channel is 100-200 mu m; the detection liquid pool is cylindrical, and the radius of the bottom surface is 2.5-3 mm.
2. The micro-raman spectrum micro-fluidic chip according to claim 1, wherein the micro-raman spectrum micro-fluidic chip is in a shape of a cuboid, and has a length of 5-6cm, a width of 2-3cm, and a height of 0.5-1 cm.
3. The micro-fluidic chip for micro-raman spectroscopy according to claim 1, wherein the SERS reagent is silver sol, and the aggregating agent is sodium chloride solution.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114917971A (en) * | 2022-05-19 | 2022-08-19 | 合肥工业大学 | Micro-fluidic chip for trace detection of hydrogen sulfide based on micro-droplets and detection method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114917971A (en) * | 2022-05-19 | 2022-08-19 | 合肥工业大学 | Micro-fluidic chip for trace detection of hydrogen sulfide based on micro-droplets and detection method |
| CN114917971B (en) * | 2022-05-19 | 2023-10-31 | 合肥工业大学 | Microfluidic chip for detecting trace hydrogen sulfide based on micro-droplets and detection method |
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