CN214457885U

CN214457885U - Chip assembly for digital PCR detection platform

Info

Publication number: CN214457885U
Application number: CN202023036174.3U
Authority: CN
Inventors: 张燕; 季京成; 许阐; 张辉; 原治英; 刘洁; 孙雨秋; 苏航
Original assignee: Hainan University
Current assignee: Hainan University
Priority date: 2020-12-16
Filing date: 2020-12-16
Publication date: 2021-10-22
Anticipated expiration: 2030-12-16

Abstract

The utility model relates to the technical field of medical equipment, concretely relates to digit PCR chip subassembly for testing platform, including examining test table, chip base, liquid drop generation part and liquid drop collection part. By arranging a liquid drop generating part, micro liquid drops are generated by adopting a flow focusing method; in the process of generating the liquid drops, the uniformity of the liquid drops is realized by controlling the flow velocity of the continuous phase and the flow velocity of the disperse phase, the generated diameter of the liquid drops is directly determined by adjusting the flow velocity of the continuous phase and the width of each channel, and the uniform and stable liquid drops are finally obtained. The liquid drops are collected by a multi-channel collection method, so that the liquid drops have speed buffering when entering the collection cavity, and bubbles at the corners of the inlet of the collection cavity are effectively prevented; meanwhile, a plurality of supporting columns are arranged in the collecting cavity, so that the problem of collapse of the collecting cavity can be avoided, liquid drops can be arranged in the collecting cavity more closely, and adverse effects caused by bubbles and the like during subsequent heating amplification are reduced.

Description

Chip assembly for digital PCR detection platform

Technical Field

The utility model relates to the technical field of medical equipment, concretely relates to digit chip subassembly for PCR testing platform.

Background

The digital PCR technology is used as a new generation of nucleic acid molecule absolute quantification technology, and takes Polymerase Chain Reaction (PCR) as a biological basis, nucleic acid templates are diluted and distributed into a large number of independent Reaction units, so that only a single template molecule exists in each Reaction unit, and then PCR amplification Reaction is carried out; and after the amplification is finished, the fluorescence signal of each reaction unit is subjected to statistical analysis, and the DNA amplification amount is quantitatively analyzed. With the technology becoming mature, the digital PCR technology plays an increasingly important role in the medical and biotechnological fields of cancer detection, transgene analysis, organ transplantation, prenatal diagnosis and the like. In the prior art, before optical imaging is performed on a droplet, a liquid containing the droplet is firstly introduced into a droplet storage chamber, and the droplet in the droplet storage chamber is heated; however, the problem of non-uniform micro-droplet size is easily caused in the droplet formation process, and in addition, gas is dissolved in the liquid entering the droplet storage cavity, and the gas expands rapidly after being heated, so that bubbles are irregularly generated at random positions of the storage cavity, the bubbles cannot be discharged and part of micro-droplets can be shielded, and therefore uniform spreading of the droplets and subsequent fluorescence detection results are influenced.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a digit PCR chip subassembly for testing platform to solve the prior art problem that exists among the above-mentioned background art.

For solving the above technical problem, the utility model provides a technical scheme does: the chip assembly for the digital PCR detection platform comprises a detection platform, a chip base, a liquid drop generation part and a liquid drop collection part; the chip base is arranged on the detection platform and is in sliding connection with the detection platform, the liquid drop generation part and the liquid drop collection part are both fixedly arranged on the chip base, and the liquid drop generation part is in through connection with the liquid drop collection part;

the liquid drop generating part comprises an oil hole, a reagent hole, a continuous phase channel, a disperse phase channel and a liquid drop outlet pipeline, wherein the oil hole is communicated with the continuous phase channel, the reagent hole is communicated with the disperse phase channel, the continuous phase channel is communicated with the disperse phase channel and forms a cross structure, and the liquid drop outlet pipeline is communicated with the cross structure;

the liquid drop collecting part comprises a collecting pipeline, a collecting cavity and a supporting column, one end of the collecting pipeline is in through connection with the liquid drop outlet pipeline, the other end of the collecting pipeline is in through connection with the collecting cavity, the supporting column is fixedly arranged in the collecting cavity, and an outlet is formed in one side of the collecting cavity.

On the basis of the technical scheme, the continuous phase channel is provided with two branch pipes, one end of each branch pipe is connected with the oil hole, and the other end of each branch pipe is communicated with the dispersed phase channel.

On the basis of the technical scheme, the collecting pipeline comprises a plurality of branch pipelines, one end of each branch pipeline is communicated with the liquid drop outlet pipeline, and the other end of each branch pipeline is connected with the collecting cavity.

On the basis of the technical scheme, the supporting columns are arranged in the collecting cavity in a plurality of uniform arrangement.

On the basis of the technical scheme, the chip base further comprises a heating device, and the heating device is fixedly arranged at the bottom end of the chip base.

On the basis of the technical scheme, the heating device comprises a heat source, a heater and a heat transfer plate, the heat source is fixedly arranged on the detection table, the heater is fixedly arranged at the top end of the heat source and is electrically connected with the heat source, one side of the heat transfer plate is arranged at the top end of the heater, and the other side of the heat transfer plate is arranged at the bottom end of the collection cavity.

On the basis of the technical scheme, the heater is a double-spiral heater.

The utility model provides a beneficial effect that technical scheme produced lies in:

1. in the utility model, the liquid drop generating part is arranged, and the micro liquid drops are generated by adopting a flow focusing method; in the process of generating the liquid drops, the uniformity of the liquid drops is realized by controlling the flow velocity of the continuous phase and the flow velocity of the disperse phase, the generated diameter of the liquid drops is directly determined by adjusting the flow velocity of the continuous phase and the width of each channel, and the uniform and stable liquid drops are finally obtained.

2. The utility model collects the liquid drops by a multi-channel collection method, so that the liquid drops have speed buffer when entering the collection cavity, and the air bubbles at the inlet corners of the collection cavity are effectively prevented; meanwhile, a plurality of supporting columns are arranged in the collecting cavity, so that the problem of collapse of the collecting cavity can be avoided, liquid drops can be arranged in the collecting cavity more closely, and adverse effects caused by bubbles and the like during subsequent heating amplification are reduced.

3. The utility model carries out heating and heat preservation on micro-droplets by arranging the heating device, and more preferably, a double helix annular heater is selected from the heating device; the temperature requirement of the chip assembly can be met, and the temperature difference of the chip during temperature rise can be ensured to be small, so that effective amplification of nucleic acid molecules in the whole cyclic heating process is guaranteed, and the detection precision is improved.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic structural view of a chip base and a droplet collecting part according to the present invention;

FIG. 3 is a schematic structural view of a droplet generating section according to the present invention;

FIG. 4 is a schematic view of the structure of the liquid droplet collecting part of the present invention;

FIG. 5 is a schematic view of the heating apparatus according to the present invention;

FIG. 6 is a schematic structural view of a double-spiral heater according to the present invention;

Detailed Description

The invention will be further explained with reference to the following figures and examples:

in the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In the description of the present invention, it is to be understood that the terms "left", "right", "front", "back", "top", "bottom", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

As shown in fig. 1 to 6, a chip assembly for a digital PCR detection platform comprises a detection platform 1, a chip base 2, a droplet generation part 3 and a droplet collection part 4; the chip base 2 is arranged on the detection platform 1 and is connected with the detection platform 1 in a sliding mode, the liquid drop generating part 3 and the liquid drop collecting part 4 are fixedly arranged on the chip base 2, and the liquid drop generating part 3 is in through connection with the liquid drop collecting part 4.

As shown in fig. 3, the droplet generating part 3 includes an oil hole 31, a reagent hole 32, a continuous phase channel 33, a dispersed phase channel 34, and a droplet outlet conduit 35, the oil hole 31 is connected to the continuous phase channel 33, the reagent hole 32 is connected to the dispersed phase channel 34, the continuous phase channel 33 is connected to the dispersed phase channel 34, a cross structure 36 is formed, and the droplet outlet conduit 35 is connected to the cross structure 36. On the basis of the technical scheme, the continuous phase channel 33 is provided with two branch pipes 37, one end of each branch pipe 37 is connected with the oil hole 31, and the other end of each branch pipe 37 is communicated with the dispersed phase channel 34. By arranging the droplet generating part 3, micro droplets are generated by adopting a flow focusing method; in the process of generating the liquid drops, the uniformity of the liquid drops is realized by controlling the flow velocity of the continuous phase and the flow velocity of the disperse phase, and the generated diameter of the liquid drops is directly determined by adjusting the flow velocity of the continuous phase and the width of each channel, so that uniform and stable liquid drops are finally obtained.

As shown in fig. 2 and 4, the droplet collecting part 4 includes a collecting pipe 41, a collecting cavity 42 and a supporting column 43, one end of the collecting pipe 41 is connected to the droplet outlet pipe 35, the other end is connected to the collecting cavity 42, the supporting column 43 is fixedly disposed in the collecting cavity 42, and an outlet 44 is opened at one side of the collecting cavity 42. The utility model discloses in collect the liquid drop through using multichannel collection method, can make the liquid drop have speed buffering when getting into collection chamber 42, prevent effectively that collection chamber 42 entry corner has the production of bubble, improve and detect precision and detection quality.

On the basis of the technical scheme, the collecting pipeline 41 comprises a plurality of branch pipelines 45, one end of each branch pipeline 45 is communicated with the liquid drop outlet pipeline 35, and the other end of each branch pipeline 45 is connected with the collecting cavity 42; this allows for more efficient dispersion of the resulting micro-droplets, thereby achieving a tighter arrangement of micro-droplets within the collection chamber 42. On the basis of the technical scheme, a plurality of supporting columns 43 are uniformly arranged in the collecting cavity 42; therefore, on one hand, the problem of collapse of the collection cavity can be avoided, on the other hand, the liquid drops can be more closely arranged in the collection cavity 42, and the adverse effects caused by bubbles and the like during subsequent heating amplification are reduced.

On the basis of the technical scheme, the chip base further comprises a heating device 5, and the heating device 5 is fixedly arranged at the bottom end of the chip base 2. In addition to the above technical solution, as shown in fig. 5, the heating device 5 includes a heat source 51, a heater 52 and a heat transfer plate 53, wherein the heat source 51 is fixedly disposed on the detection table 1, the heater 52 is fixedly disposed at a top end of the heat source 51 and electrically connected to the heat source 51, one side of the heat transfer plate 53 is disposed at a top end of the heater 52, and the other side is disposed at a bottom end of the collection chamber 42. The collection chamber 42 can be heated and insulated by providing the heating device 5. More preferably, as shown in fig. 6, the heater 52 is a double-spiral heater, which not only can meet the temperature requirement of the chip assembly, but also can ensure that the temperature difference is small when the temperature is raised, thereby ensuring that the nucleic acid molecules are effectively amplified in the whole cyclic heating process, and improving the detection precision.

The basic principles and the main features of the invention and the advantages of the invention have been shown and described above, it will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, but that the invention may be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. A chip component for a digital PCR detection platform is characterized by comprising a detection platform (1), a chip base (2), a liquid drop generation part (3) and a liquid drop collection part (4); the chip base (2) is arranged on the detection platform (1) and is in sliding connection with the detection platform (1), the liquid drop generation part (3) and the liquid drop collection part (4) are both fixedly arranged on the chip base (2), and the liquid drop generation part (3) is in through connection with the liquid drop collection part (4);

the droplet generation part (3) comprises an oil hole (31), a reagent hole (32), a continuous phase channel (33), a dispersed phase channel (34) and a droplet outlet pipeline (35), the oil hole (31) is communicated with the continuous phase channel (33), the reagent hole (32) is communicated with the dispersed phase channel (34), the continuous phase channel (33) is communicated with the dispersed phase channel (34) and forms a cross-shaped structure (36), and the droplet outlet pipeline (35) is communicated with the cross-shaped structure (36);

the liquid drop collecting part (4) comprises a collecting pipeline (41), a collecting cavity (42) and a supporting column (43), one end of the collecting pipeline (41) is communicated with the liquid drop outlet pipeline (35), the other end of the collecting pipeline is communicated with the collecting cavity (42), the supporting column (43) is fixedly arranged in the collecting cavity (42), and an outlet (44) is formed in one side of the collecting cavity (42).

2. The chip assembly for the digital PCR detection platform according to claim 1, wherein the continuous phase channel (33) is provided as two branch pipes (37), one end of each branch pipe (37) is connected with the oil hole (31), and the other end of each branch pipe (37) is connected with the dispersed phase channel (34) in a penetrating manner.

3. The chip assembly for digital PCR detection platform according to claim 1, wherein the collection channel (41) comprises a plurality of branch channels (45), one end of each branch channel (45) is connected with the droplet outlet channel (35) in a through manner, and the other end of each branch channel is connected with the collection cavity (42).

4. The chip assembly for digital PCR detection platform according to claim 1, wherein the supporting columns (43) are arranged in a plurality and uniform arrangement in the collection cavity (42).

5. The chip assembly for the digital PCR detection platform according to claim 1, further comprising a heating device (5), wherein the heating device (5) is fixedly arranged at the bottom end of the chip base (2).

6. The chip assembly for digital PCR detection platform according to claim 5, wherein the heating device (5) comprises a heat source (51), a heater (52) and a heat transfer plate (53), the heat source (51) is fixedly arranged on the detection platform (1), the heater (52) is fixedly arranged at the top end of the heat source (51) and is electrically connected with the heat source (51), and one side of the heat transfer plate (53) is arranged at the top end of the heater (52) and the other side is arranged at the bottom end of the collection cavity (42).

7. The chip assembly for digital PCR detection platform according to claim 6, wherein the heater (52) is configured as a double helix heater.