CN214422658U - Cell culture device - Google Patents

Cell culture device Download PDF

Info

Publication number
CN214422658U
CN214422658U CN202120257646.3U CN202120257646U CN214422658U CN 214422658 U CN214422658 U CN 214422658U CN 202120257646 U CN202120257646 U CN 202120257646U CN 214422658 U CN214422658 U CN 214422658U
Authority
CN
China
Prior art keywords
cell
edge
cell culture
culture
climbing piece
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202120257646.3U
Other languages
Chinese (zh)
Inventor
肖波
李良贤
姚冬
莫碧文
侯丽霞
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guilin Medical University
Original Assignee
Guilin Medical University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guilin Medical University filed Critical Guilin Medical University
Priority to CN202120257646.3U priority Critical patent/CN214422658U/en
Application granted granted Critical
Publication of CN214422658U publication Critical patent/CN214422658U/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Landscapes

  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model relates to a cell culture technical field specifically discloses a cell culture device, establish the lid in the cultivation board top including cultivation board and cover, the upper surface equipartition of cultivation board is provided with a plurality of cultivation holes, cultivates downthehole being provided with rather than size matched with cell climbing piece, and the edge of cell climbing piece is provided with the diaphragm, and the diaphragm includes connecting portion and clamping part, and connecting portion are used for with cell climbing piece fixed connection, and the one end of clamping part links to each other with connecting portion, and the other end upwards extends by the edge of cell climbing piece to protruding top of establishing at the cell climbing piece. The utility model discloses a cell culture device is convenient for take out cell climbing piece from cultivateing the hole.

Description

Cell culture device
Technical Field
The utility model relates to a cell culture technical field especially relates to a cell culture device.
Background
At present, when carrying out cell immunofluorescence and immunochemistry research, need cultivate the cell through the cell culture plate, be provided with a plurality of culture holes on the cell culture plate, the cell slide that is used for cultivating the cell has all been placed in every culture hole, after the cultivation is accomplished, need take out the cell slide from culture hole and observe under the microscope, but the bottom surface of culture hole and cell slide is bright and clean plane usually for the tight laminating of cell slide is on the bottom plate, be difficult to take out from culture hole, and the cell slide is generally thinner, increase the degree of difficulty of taking out, in the in-process of taking out exert oneself too big still easily damage the cell slide.
Disclosure of Invention
The utility model discloses aim at solving one of the above-mentioned technical problem at least, provide a cell culture device, be convenient for take out cell climbing piece from cultivateing the hole.
In order to realize the purpose, the utility model discloses a technical scheme be: the utility model provides a cell culture device, establishes the lid in cultivation board top including cultivateing board and cover, and the upper surface equipartition of cultivateing the board is provided with a plurality of cultivation holes, cultivates downthehole being provided with rather than size matched with cell climbing piece, and the edge of cell climbing piece is provided with the diaphragm, and the diaphragm includes connecting portion and clamping part, and connecting portion are used for with cell climbing piece fixed connection, and the one end of clamping part links to each other with connecting portion, and the other end upwards extends by the edge of cell climbing piece to protruding top of establishing at the cell climbing piece.
Preferably, the membrane is made of plastic.
Preferably, the membrane has a thickness of 0.05-0.1 mm.
Preferably, the connecting part is fixedly connected with the cell climbing sheet in an adhesion mode.
Preferably, the connecting part is adhered and fixed on the side wall of the edge of the cell climbing sheet.
Preferably, the connecting part is bonded and fixed to the edge of the bottom surface of the cell climbing sheet.
Preferably, the height of the clamping part protruding out of the upper surface of the cell climbing sheet is 3-5 mm.
Preferably, the bottom surface of the cover body is provided with a groove corresponding to each culture hole one by one.
Preferably, the top edge of the culture plate is provided with a clamping groove, and the edge of the cover body is provided with a flange matched with the clamping groove.
Preferably, the culture plate and the cover are both made of a transparent material.
The beneficial effects are that: compared with the prior art, the utility model discloses a cell culture device is provided with the diaphragm through the edge at the cell climbing piece, and is provided with the protruding clamping part of establishing in cell climbing piece top on the diaphragm to carry the clamping part through tweezers, thereby take out the cell climbing piece from cultivateing the hole, easy operation is convenient, has improved the efficiency of taking out of cell climbing piece, and has avoided causing the condition of damage to the cell climbing piece at the in-process of taking out.
Drawings
The following detailed description of embodiments of the invention is provided in conjunction with the accompanying drawings, in which:
FIG. 1 is a schematic diagram of a cell culture apparatus according to the present application;
FIG. 2 is a schematic view of the internal structure of a cell culture apparatus according to the present application;
FIG. 3 is a schematic view of one of the connection structures of the patch and the cell slide;
FIG. 4 is a schematic view of another connection structure of the patch and the cell slide.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When a component is referred to as being "connected" to another component, it can be directly connected to the other component or intervening components may also be present. When a component is referred to as being "disposed on" another component, it can be directly on the other component or there can be intervening components, and when a component is referred to as being "disposed in the middle," it is not just disposed in the middle, so long as it is not disposed at both ends, but rather is within the scope of the middle. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
As shown in fig. 1 to 4, a cell culture apparatus comprises a culture plate 1 and a cover 2 covering the culture plate 1, wherein a plurality of culture holes 3 are uniformly distributed on the upper surface of the culture plate 1, a groove 8 is provided on the bottom surface of the cover 2 corresponding to each culture hole 3, preferably, the culture holes 3 are circular, a cell climbing sheet 4 with a size matched with the culture holes 3 is arranged in each culture hole 3, a membrane 5 is fixedly arranged on the edge of the cell climbing sheet 4, the membrane 5 comprises a connecting part 6 and a clamping part 7, the connecting part 6 is fixedly connected with the cell climbing sheet 4, one end of the clamping part 7 is connected with the connecting part 6, the other end extends upwards from the edge of the cell climbing sheet 4 to be convexly arranged above the cell climbing sheet 4, specifically, the connecting part 6 can be fixedly connected with the cell climbing sheet 4 by bonding, the membrane 5 can be made of plastic, the thickness of the membrane 5 can be 0.05-0.1mm, to facilitate gripping by the forceps.
The utility model discloses a cell culture device, through being provided with diaphragm 5 at the edge of cell slide 4, and be provided with protruding clamping part 7 of establishing above cell slide 4 on diaphragm 5, when operating personnel takes cell slide 4 out of cultivateing hole 3 again, can clip clamping part 7 through tweezers, thereby take cell slide 4 out of cultivateing hole 3, easy and convenient operation has improved the efficiency of taking out of cell slide 4, and avoided causing the condition of damage to cell slide 4 in the process of taking out, cell slide 4 is taken out simultaneously, place under the microscope, or when other horizontal mesa, also can be convenient to carry out the clamp through clamping part 7 and get and move, in addition, in prior art, in order to facilitate taking out of cell slide 4 in cultivateing hole 3, the internal diameter of cultivateing hole 3 sets up greatly usually, so that cell slide 4 gets to leave great clearance between the edge and the inner wall of cultivateing hole 3, so that the clamp of cell climbing piece 4 gets, in this application, through be provided with on diaphragm 5 and press from both sides the portion of getting, make things convenient for the clamp of tweezers to get, consequently cultivate the internal diameter of hole 3 slightly big with the external diameter of cell climbing piece 4 can, on the culture plate 1 of the same size, can set up more cultivation holes 3.
In one embodiment, as shown in fig. 3, the connecting part 6 can be adhered and fixed on the side wall of the edge of the cell slide 4, and the connecting part 6 is adhered on the side wall of the cell slide 4, so that the bottom surface of the cell slide 4 can be completely attached to the bottom surface of the culture hole 3, thereby reducing the possibility that cells will not propagate on the bottom surface of the cell slide 4.
In another embodiment, as shown in fig. 4, the connecting portion 6 can be adhered and fixed on the edge of the bottom surface of the cell-climbing sheet 4, and the connecting portion 6 can be adhered and fixed on the edge of the bottom surface of the cell-climbing sheet 4 to facilitate the connection and fixation of the membrane 5, and in order to improve the flatness of the bottom surface of the cell-climbing sheet 4 after the membrane 5 is adhered, the edge of the bottom surface of the cell-climbing sheet 4 can be provided with a concave portion which is matched with the connecting portion 6, so that the surface of the connecting portion 6, which faces away from the concave portion, is flush with the lower surface of the cell-climbing sheet 4 after the connecting portion is adhered and fixed.
In a preferred embodiment, the height of the upper surface of the cell slide 4 protruded from the holding portion 7 can be 3-5mm, so as to facilitate the holding of the holding portion 7 by tweezers.
More specifically, cultivate board 1 and lid 2 and be square setting, and the top edge of cultivateing board 1 is provided with draw-in groove 9, the edge of lid 2 be provided with draw-in groove 9 complex flange 10, through flange 10 and draw-in groove 9 cooperation of pegging graft, improve the stability that lid 2 and cultivation board 1 are connected, for the convenience of observing the condition in cultivateing board 1 simultaneously, cultivate board 1 and lid 2 and can make by transparent material, and is specific, can all adopt transparent ya keli material to make.
The above embodiments are only used for illustrating the technical solutions of the present invention and are not limited thereto, and any modification or equivalent replacement that does not depart from the spirit and scope of the present invention should be covered by the scope of the technical solutions of the present invention.

Claims (10)

1. The utility model provides a cell culture device, a serial communication port, establish lid (2) in culture plate (1) top including culture plate (1) and cover, the upper surface equipartition of culture plate (1) is provided with a plurality of culture holes (3), be provided with in culture hole (3) with its size matched with cell climbing piece (4), the edge of cell climbing piece (4) is provided with diaphragm (5), diaphragm (5) are including connecting portion (6) and clamping part (7), connecting portion (6) are used for with cell climbing piece (4) fixed connection, the one end of clamping part (7) links to each other with connecting portion (6), the other end is upwards extended by the edge of cell climbing piece (4), with protruding the top of establishing in cell climbing piece (4).
2. A cell culture device according to claim 1, wherein the membrane (5) is made of plastic.
3. A cell culture device according to claim 1, wherein the membrane (5) has a thickness of 0.05-0.1 mm.
4. A cell culture device according to claim 1, wherein the connecting part (6) is fixedly connected with the cell slide (4) by means of adhesion.
5. A cell culture device according to claim 4, wherein the connecting part (6) is adhesively fixed on the side wall of the edge of the cell slide (4).
6. A cell culture device according to claim 4, wherein the connecting part (6) is adhesively fixed to the edge of the bottom surface of the cell slide (4).
7. The cell culture apparatus according to claim 1, wherein the height of the upper surface of the cell slide (4) projected from the holding portion (7) is 3 to 5 mm.
8. A cell culture apparatus according to claim 1, wherein the bottom surface of the cover (2) is provided with a recess (8) for each culture well (3).
9. A cell culture device according to claim 1, wherein the top edge of the culture plate (1) is provided with a slot (9), and the edge of the cover (2) is provided with a flange (10) that fits into the slot (9).
10. A cell culture device according to claim 1, wherein the culture plate (1) and the cover (2) are made of a transparent material.
CN202120257646.3U 2021-01-29 2021-01-29 Cell culture device Active CN214422658U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202120257646.3U CN214422658U (en) 2021-01-29 2021-01-29 Cell culture device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202120257646.3U CN214422658U (en) 2021-01-29 2021-01-29 Cell culture device

Publications (1)

Publication Number Publication Date
CN214422658U true CN214422658U (en) 2021-10-19

Family

ID=78057453

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202120257646.3U Active CN214422658U (en) 2021-01-29 2021-01-29 Cell culture device

Country Status (1)

Country Link
CN (1) CN214422658U (en)

Similar Documents

Publication Publication Date Title
CN214422658U (en) Cell culture device
JP2014075979A (en) Cell sheet transplantation jig and use method thereof
AU2002349234A1 (en) Milk protein biofilm and uses thereof
CN208663565U (en) A kind of glue frame localization tool
CN206274683U (en) A kind of mould of vibrating diaphragm shaping
CN212693412U (en) Pathological diagnosis sample embedding device
CN106514015A (en) Laser marking system and positioning tool thereof
CN206273464U (en) Laser marking system and its positioning tool
CN217699253U (en) Cell climbing sheet holder
CN218624944U (en) Auxiliary material attaching jig for wearable equipment display screen
CN217324126U (en) A novel orifice plate for cell culture
CN212379141U (en) Device for immunocytochemistry staining
CN211771358U (en) Plant is with cultivateing board connection structure convenient to dismantle and observe
CN206990301U (en) A kind of six pore membrane formula pelleters
CN217431772U (en) Deep cone bottom plate for square hole
CN211686315U (en) Storage box capable of protecting front end of cutter for cutter
CN215103323U (en) Can make up cell culture orifice plate of dismantlement
CN218710553U (en) Novel six orifice plates
CN219064832U (en) Carrier for lens test
CN210528955U (en) Cell culture dish
CN219951069U (en) Multi-layer biological culture bottle
CN209741162U (en) Cell culture dish convenient to separation
CN218404283U (en) Cell culture plate convenient to separate
CN210287377U (en) Molecular biology culture dish
CN220887508U (en) Easy-to-lift bacterial cell shake flask

Legal Events

Date Code Title Description
GR01 Patent grant
GR01 Patent grant