CN214422654U

CN214422654U - Culture apparatus for in vitro construction of pig embryo

Info

Publication number: CN214422654U
Application number: CN202023194458.5U
Authority: CN
Inventors: 屠平光; 章啸君; 楼芳芳; 黄剑锋; 胡旭进; 项云; 杜喜忠; 赵静帆
Original assignee: Jinhua Academy of Agricultural Sciences
Current assignee: Jinhua Academy of Agricultural Sciences
Priority date: 2020-12-25
Filing date: 2020-12-25
Publication date: 2021-10-19
Anticipated expiration: 2030-12-25

Abstract

The utility model discloses an external culture apparatus who founds embryo of pig relates to animal embryo culture field, the power distribution box comprises a box body, miniature vacuum air pump is installed to the upper surface one end of box, and the upper surface other end of box installs sealed observation mechanism, the opening has been seted up at the upper surface middle part of box, and the electric cabinet is installed to the front surface one end of box. The utility model seals and observes the culture dish through the sealed observation mechanism, and the sealed observation mechanism can observe and record the embryo development in the culture dish under the condition of not opening the culture dish cover; through the indoor temperature of electric cabinet control temperature control, utilize the warm water to come to carry out the heat conduction to the culture dish that submerges, because the specific heat capacity of water is great, self temperature variation is less when emitting great heat, and then when the heater damages or has a power failure, can ensure for a long time that the embryo in the culture dish is in a constant temperature state always, and the staff also has arrived to solve in addition and is troublesome.

Description

Culture apparatus for in vitro construction of pig embryo

Technical Field

The utility model relates to a field specifically is a pig constructs embryonal culture apparatus in vitro.

Background

The utility model discloses embryo external culture puts the embryo in special container promptly and puts 37 degrees centigrade (add artifical mist sometimes), makes the external continuous development of embryo, and is stricter to conditions such as culture solution and aseptic, and this method is often used for distinguishing embryo survival rate and carries out the external research of embryo.

Present embryo culture device simple structure, the inconvenient embryo condition of observing when sealed embryo culture, open and observe and lead to the air admission culture dish easily, bacterium in the air has very big influence to embryo survival rate, secondly accurate inadequately in the aspect of the control by temperature change, the optimum temperature that the embryo survived is 37, current embryo culture accuse temperature mode is to the culture dish outside air heating accuse temperature, and when the circuit breaks down, can't keep temperature variation little for the short time, and then influence embryo survival rate.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a: in order to solve the problem, a culture device for in vitro construction of the embryo of the pig is provided.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides an external embryonal culture apparatus that founds of pig, the power distribution box comprises a box body, miniature vacuum air pump is installed to the upper surface one end of box, and the upper surface other end of box installs sealed observation mechanism, the opening has been seted up at the upper surface middle part of box, and the front surface one end of box installs the electric cabinet, the culture dish has been placed to the inboard of opening, the outer wall upper end of culture dish is provided with the solid fixed ring of culture dish, the inside of box is close to the opening under the position department be provided with the temperature control room, the heater is installed to the lower extreme of temperature control room, and the one end of temperature control room is provided with the water inlet, the one end of temperature control room is close to the water inlet under the position department and installs temperature probe, and the other end of temperature control room is provided with the delivery port.

Wherein, sealed observation mechanism is including electric telescopic handle, culture dish lid, gas pocket, microscope and connecting block, electric telescopic handle is located one side position department that the upper surface other end of box is close to the opening, connecting block fixed connection is in electric telescopic handle's flexible end, microscope fixed connection is in the one end of connecting block, the culture dish lid is located microscopical lower extreme, the gas pocket is located culture dish lid upper surface one end and is close to microscope one side position department.

Preferably, electric telescopic handle passes through bolt fixed connection with the box, electric telescopic handle passes through welded fastening with the connecting block and is connected, microscope and culture dish lid pass through the screw thread and rotate and be connected, and the junction of microscope and culture dish lid is provided with the sealing washer.

Preferably, the culture dish lid is directly over the culture dish, the sealing washer has been added to the butt joint department of culture dish lid and culture dish, the external diameter of culture dish slightly is less than the internal diameter of opening, the solid fixed ring's of culture dish external diameter is greater than the internal diameter of opening.

Preferably, the air outlet end of the micro vacuum air pump is connected with a first conduit, and a second conduit is connected between the air exhaust end of the micro vacuum air pump and the air hole.

Preferably, the temperature control chamber is filled with purified water, and valves are mounted on the water inlet and the water outlet.

Preferably, the front surface of the electric cabinet is provided with a display screen, the electric cabinet is internally provided with a temperature controller, a temperature sensor, a PLC (programmable logic controller) and a relay, and the display screen is provided with an electronic thermometer.

Compared with the prior art, the beneficial effects of the utility model are that: the utility model seals and observes the culture dish through the sealed observing mechanism, the embryo can not contact with the outside air too much in the process of culture, and the sealed observing mechanism can observe and record the development of the embryo in the culture dish under the condition of not opening the cover of the culture dish; through the indoor temperature of electric cabinet control temperature control, utilize the warm water to come to carry out the heat conduction to the culture dish that submerges, embryo thermally equivalent in the culture dish, the temperature can keep invariable for a long time, because the specific heat capacity of water is great, self temperature variation is less when emitting a large amount of heat, and then when the heater damages or has a power failure, can ensure for a long time that the embryo in the culture dish is in a constant temperature state always, in addition the staff also has arrived and solves the trouble.

Drawings

Fig. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of the internal structure of the case of the present invention;

fig. 3 is a schematic structural view of the sealing observation mechanism of the present invention.

In the figure: 1. a box body; 2. an electric cabinet; 3. a temperature measuring probe; 4. a water inlet; 5. a port; 6. a first conduit; 7. a micro vacuum air pump; 8. a culture dish; 9. a second conduit; 10. a sealed viewing mechanism; 101. an electric telescopic rod; 102. a culture dish cover; 103. air holes; 104. a microscope; 105. connecting blocks; 11. a culture dish fixing ring; 12. a water outlet; 13. a heater; 14. and a temperature control chamber.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted", "connected" and "disposed" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art. The following describes an embodiment of the present invention according to its overall structure.

Please refer to fig. 1-3, a culture apparatus for constructing embryos outside pigs, comprising a box body 1, wherein a micro vacuum air pump 7 is installed at one end of the upper surface of the box body 1, a sealing observation mechanism 10 is installed at the other end of the upper surface of the box body 1, an opening 5 is formed in the middle of the upper surface of the box body 1, an electric cabinet 2 is installed at one end of the front surface of the box body 1, a culture dish 8 is placed inside the opening 5, a culture dish fixing ring 11 is arranged at the upper end of the outer wall of the culture dish 8, a temperature control chamber 14 is arranged at the position, right below, close to the opening 5, of the inside of the box body 1, a heater 13 is installed at the lower end of the temperature control chamber 14, a water inlet 4 is arranged at one end of the temperature control chamber 14, a temperature measuring probe 3 is installed at the position, right below, close to the water inlet 4, and a water outlet 12 is arranged at the other end of the temperature control chamber 14.

Wherein, sealed observation mechanism 10 is including electric telescopic handle 101, culture dish lid 102, gas pocket 103, microscope 104 and connecting block 105, and electric telescopic handle 101 is located one side position department that the upper surface other end of box 1 is close to opening 5, and connecting block 105 fixed connection is in electric telescopic handle 101's flexible end, and microscope 104 fixed connection is in connecting block 105's one end, and culture dish lid 102 is located microscope 104's lower extreme, and gas pocket 103 is located culture dish lid 102 upper surface one end and is close to microscope 104 one side position department.

As the preferred embodiment of the utility model, electric telescopic handle 101 passes through bolt fixed connection with box 1, and electric telescopic handle 101 passes through welded fastening with connecting block 105 and is connected, and microscope 104 passes through the screw thread rotation with culture dish lid 102 and is connected, and the junction of microscope 104 and culture dish lid 102 is provided with the sealing washer, improves the leakproofness of culture dish 8 to and can observe with microscope 104 at the in-process of cultivateing.

As the utility model discloses a preferred embodiment, culture dish lid 102 is in directly over culture dish 8, and culture dish lid 102 has set up the sealing washer with the butt joint department of culture dish 8 additional, and the external diameter of culture dish 8 slightly is less than the internal diameter of opening 5, and the solid fixed ring 11's of culture dish external diameter is greater than the internal diameter of opening 5, the accurate butt joint of culture dish 8 and culture dish lid 102 of can being convenient for.

As the preferred embodiment of the utility model, the air outlet end of the miniature vacuum air pump 7 is connected with the first conduit 6, the second conduit 9 is connected between the air exhaust end of the miniature vacuum air pump 7 and the air hole 103, and the part of air in the culture dish 8 is extracted by the miniature vacuum air pump 7 through the control of the accessible.

As the preferred embodiment of the utility model, the inside packing of temperature controlled chamber 14 has the pure water, all installs the valve on water inlet 4 and the delivery port 12, can be convenient for add water or drain to the temperature controlled chamber.

As the utility model discloses a preferred embodiment, the front surface mounting of electric cabinet 2 has the display screen, and the internally mounted of electric cabinet 2 has temperature controller, temperature-sensing ware, PLC controller and relay, installs electronic thermometer on the display screen, for more accurate control temperature.

The working principle is as follows: when the utility model is used, put into opening 5 with the culture dish of embryo and culture solution earlier, control electric telescopic handle 101 shrink after that, drive culture dish lid 102 and descend slowly, until culture dish lid 102 detains on culture dish 8 completely, the inside of interior culture dish 8 is in a sealed environment, part air in culture dish 8 is taken out through controlling miniature vacuum air pump 7, also pack isovolumic pure mist (this mist is 5% CO2, 5% O2, 90% N2 or 5% CO2, 95% air) in culture dish 8 through miniature vacuum air pump 7, after culture dish 8 is sealed, heat the accuse temperature through heater 13 to the pure water in the control by temperature change room 14, until the temperature keeps at 37 degrees centigrade, the lower extreme of culture dish 8 does not go into the aquatic, the culture dish 8 is wrapped up to the warm water, and supply the temperature to the embryo in culture dish 8 continuously, the temperature can keep invariable for a long time, because the specific heat capacity of water is great, self temperature variation is less when emitting a large amount of heat, and then when heater 13 damages or has a power failure, can guarantee for a long time that the embryo in culture dish 8 is in a constant temperature state always, and the staff also has to reach to solve in addition troublesome, the utility model discloses during to embryo culture, microscope 104 on the accessible culture dish lid 102 observes, knows the developmental situation of cultivateing in the culture dish 8 and takes notes, and then continuous save external culture experience, the continuous improvement embryo survival rate.

It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. The utility model provides a pig constructs culture apparatus of embryo in vitro, includes box (1), its characterized in that: the device is characterized in that a miniature vacuum air pump (7) is installed at one end of the upper surface of the box body (1), a sealed observation mechanism (10) is installed at the other end of the upper surface of the box body (1), an opening (5) is formed in the middle of the upper surface of the box body (1), an electric cabinet (2) is installed at one end of the front surface of the box body (1), a culture dish (8) is placed on the inner side of the opening (5), a culture dish fixing ring (11) is arranged at the upper end of the outer wall of the culture dish (8), a temperature control chamber (14) is arranged in the box body (1) and close to the opening (5) at the position under the opening, a heater (13) is installed at the lower end of the temperature control chamber (14), a water inlet (4) is formed in one end of the temperature control chamber (14), a temperature measuring probe (3) is installed at the position under the water inlet (4) and a water outlet (12) is formed in the other end of the temperature control chamber (14),

wherein, sealed observation mechanism (10) is including electric telescopic handle (101), culture dish lid (102), gas pocket (103), microscope (104) and connecting block (105), one side position department that the upper surface other end that electric telescopic handle (101) are located box (1) is close to through-hole (5), connecting block (105) fixed connection is in the flexible end of electric telescopic handle (101), microscope (104) fixed connection is in the one end of connecting block (105), culture dish lid (102) are located the lower extreme of microscope (104), gas pocket (103) are located culture dish lid (102) upper surface one end and are close to microscope (104) one side position department.

2. The culture device for in vitro construction of embryos of pigs according to claim 1, wherein: electric telescopic handle (101) and box (1) pass through bolt fixed connection, electric telescopic handle (101) and connecting block (105) pass through welded fastening and are connected, microscope (104) are connected through screw thread rotation with culture dish lid (102), and the junction of microscope (104) and culture dish lid (102) is provided with the sealing washer.

3. The culture device for in vitro construction of embryos of pigs according to claim 1, wherein: the culture dish cover (102) is arranged right above the culture dish (8), a sealing ring is additionally arranged at the butt joint of the culture dish cover (102) and the culture dish (8), the outer diameter of the culture dish (8) is slightly smaller than the inner diameter of the through opening (5), and the outer diameter of the culture dish fixing ring (11) is larger than the inner diameter of the through opening (5).

4. The culture device for in vitro construction of embryos of pigs according to claim 1, wherein: the air outlet end of the miniature vacuum air pump (7) is connected with a first conduit (6), and a second conduit (9) is connected between the air exhaust end of the miniature vacuum air pump (7) and the air hole (103).

5. The culture device for in vitro construction of embryos of pigs according to claim 1, wherein: the temperature control chamber (14) is filled with purified water, and valves are mounted on the water inlet (4) and the water outlet (12).

6. The culture device for in vitro construction of embryos of pigs according to claim 1, wherein: the front surface of the electric cabinet (2) is provided with a display screen, a temperature controller, a temperature sensor, a PLC (programmable logic controller) and a relay are arranged inside the electric cabinet (2), and an electronic thermometer is arranged on the display screen.