CN214409436U - TIRF lighting system - Google Patents
TIRF lighting system Download PDFInfo
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- CN214409436U CN214409436U CN202023317480.4U CN202023317480U CN214409436U CN 214409436 U CN214409436 U CN 214409436U CN 202023317480 U CN202023317480 U CN 202023317480U CN 214409436 U CN214409436 U CN 214409436U
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- prism
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- 239000006059 cover glass Substances 0.000 claims abstract description 28
- 238000005286 illumination Methods 0.000 claims abstract description 20
- 238000007654 immersion Methods 0.000 claims abstract description 9
- 238000003384 imaging method Methods 0.000 claims description 15
- 239000005357 flat glass Substances 0.000 claims description 12
- 230000005540 biological transmission Effects 0.000 claims description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 2
- 230000002238 attenuated effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000012634 optical imaging Methods 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model discloses a TIRF lighting system, include: a light source unit and a prism; the prism is arranged right below the light source unit, one side of the prism is a curved surface, the other side of the prism is an inclined plane with a wide upper part and a narrow lower part, and the inclined plane is positioned right below the light source unit; the observation sample that awaits measuring is placed between upper cover glass and lower cover glass, and upper cover glass is located the below of prism, fills the immersion oil between upper cover glass and the prism, and prism, immersion oil and upper cover glass's refracting index are the same, and all are greater than the refracting index of the observation sample that awaits measuring. The utility model discloses can realize high energy utilization, TIRF lighting system has realized twice round illumination, has improved the energy utilization of TIRF illumination, or has reduced the light source power of TIRF illumination.
Description
Technical Field
The utility model relates to an optical imaging technical field, concretely relates to TIRF lighting system.
Background
A Total Internal Reflection Fluorescence Microscope (TIRFM), which excites fluorescent molecules to observe an extremely thin region of a fluorescence calibration sample by utilizing the characteristic that an evanescent wave is generated on the other surface of a medium after light is totally reflected, and the dynamic range of observation is usually below 200 nm. Because the intensity of evanescent waves is exponentially attenuated along the direction vertical to the interface, only a small thin layer sample area which is hundreds of nanometers near the interface can be excited, and the interference of background light noise is greatly reduced, so that the technology is widely applied to the technical field requiring high signal-to-noise ratio, and is particularly suitable for researching dynamic imaging processes of cell membranes or single molecules in the area near the membranes, such as biological single molecule research, cell signal transduction dynamic process research and the like. In the existing TIRF microscopic imaging system, the TIRF illumination angle is not large enough, the omnidirectional-angle shadow-free illumination of a sample is difficult to realize, the imaging quality of the sample is poor, and the TIRF illumination can be used only once and cannot be used for multiple times under general conditions. And the larger the TIRF angle is, the thinner the evanescent wave of illumination is, and stray light caused by the thicker evanescent wave can be better improved. Therefore, there is a need in the industry to develop a TIRF lighting system that has a larger TIRF angle and that can recycle TIRF lighting.
SUMMERY OF THE UTILITY MODEL
The utility model aims at overcoming the not enough of above prior art existence, provide one kind and realized twice round illumination, improved TIRF lighting system of the energy utilization of TIRF illumination.
The purpose of the utility model is realized through the following technical scheme:
a TIRF lighting system comprising: a light source unit and a prism; the prism is arranged right below the light source unit, one side of the prism is a curved surface, the other side of the prism is an inclined plane with a wide upper part and a narrow lower part, and the inclined plane is positioned right below the light source unit; the observation sample that awaits measuring is placed between upper cover glass and lower cover glass, and upper cover glass is located the below of prism, fills the immersion oil between upper cover glass and the prism, and prism, immersion oil and upper cover glass's refracting index are the same, and all are greater than the refracting index of the observation sample that awaits measuring.
Preferably, the light source unit includes a laser light source, a first lens disposed directly below the laser light source, a prism disposed directly below the first lens, and an inclined surface located directly below the first lens.
Preferably, the left side of the prism is a curved surface, and the right side of the prism is an inclined plane with a wide upper part and a narrow lower part.
Preferably, the TIRF lighting system further comprises: debugging the imaging assembly; the debugging imaging component comprises an imaging camera, a second lens and light splitting flat glass which are horizontally and sequentially arranged; the light splitting plate glass is obliquely arranged between the lens and the inclined plane of the prism; the surface of the light-splitting plate glass facing the first lens is plated with a semi-transmission film, and the other surface of the light-splitting plate glass is plated with a total reflection transmission film.
Compared with the prior art, the utility model have following advantage:
(1) the utility model has low requirement on the objective lens of the system, does not need a specific TIRF objective lens and can realize the fixed TIRF angle incidence; and the TIRF angular incidence range is large.
(2) The utility model discloses can realize high energy utilization, TIRF lighting system has realized twice round illumination, has improved the energy utilization of TIRF illumination, or has reduced the light source power of TIRF illumination.
Drawings
The accompanying drawings, which form a part of the present application, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention and not to limit the invention. In the drawings:
fig. 1 is a schematic structural diagram of a TIRF lighting system according to the present invention.
Fig. 2 is an optical diagram of the TIRF lighting system of the present invention.
Detailed Description
The present invention will be further explained with reference to the drawings and examples.
Referring to fig. 1, a TIRF lighting system comprising: a laser light source 101, a first lens 102, and a prism 106; the first lens 102 is arranged right below the laser light source 101, the prism 106 is arranged right below the first lens 102, the left side of the prism 106 is a curved surface, the right side is an inclined plane with a wide upper part and a narrow lower part, and the inclined plane is arranged right below the first lens 102; the observation sample that awaits measuring is placed between upper cover glass 1071 and lower cover glass 1072, and upper cover glass 1071 is located the below of prism 106, fills the immersion oil between upper cover glass 1071 and the prism 106, and prism 106, immersion oil are the same with the refracting index of upper cover glass 1071, and all are greater than the refracting index of the observation sample that awaits measuring.
Referring to fig. 2, in the light path diagram of the TIRF illumination system of the present embodiment, the laser 1061 is vertically incident on the prism 106, and after being reflected by the inclined surface, the laser 1062 is obliquely incident on the bottom surface of the prism 106, and since the refractive indexes of the prism 106, the immersion oil and the upper cover glass 1071 are the same and are all larger than the refractive index of the observation sample to be measured. The laser 1062 further obliquely enters the upper cover glass 1071 to be totally reflected, the reflected laser 1063 enters the left curved surface of the prism 106, the laser is refocused by the curved surface to form reflected light 1064, the reflected light 1064 is refocused to the position where the laser 1062 totally reflects, namely, the position where the laser 1062 enters the upper cover glass 1071 is consistent with the position where the laser 1064 enters the upper cover glass 1071, the two lights are simultaneously focused to the same position to realize TIRF illumination, the light totally reflected by the upper cover glass 1071 is laser 1065, the light is reflected by the inclined surface of the prism 106 to be laser 1066, and finally the laser 1066 is emitted out of the prism 106. The light of this scheme is through twice incidenting to the same position TIRF illumination of upper cover glass 1071, has realized the light enhancement of illumination. When light enters the upper cover glass 1071 twice and TIRF illumination is carried out at the same position, the TIRF illumination range is large, evanescent waves generated around the upper cover glass 1071 irradiate on an observation sample to be observed, and the observation of the sample is realized by imaging the observation sample to be observed at the later stage.
Since the reflected laser light 1063 is perpendicularly incident on the left curved surface of the prism 106, the left curved surface of the prism 106 is perpendicularly reflected, and the condensed reflected light 1064 is formed. The left curved surface of the prism 106 corresponds to a mirror.
In this embodiment, the TIRF lighting system further comprises: debugging the imaging assembly; the debugging imaging component comprises an imaging camera 105, a second lens 104 and a light splitting flat glass 103 which are horizontally arranged in sequence; the light splitting flat glass 103 is obliquely arranged between the lens and the inclined plane of the prism 106; the surface of the light splitting flat glass 103 facing the first lens 102 is coated with a semi-transmission film, and the other surface is coated with a total reflection transmission film. The laser light 1066 is reflected by the spectroscopic flat glass 103 to the second lens 104, and is finally imaged by the imaging camera 105. The debugging imaging component is mainly used for debugging the total reflection light spots at the bottom of the prism 106 before an experiment.
The above-mentioned specific implementation is the preferred embodiment of the present invention, can not be right the utility model discloses the limit, any other does not deviate from the technical scheme of the utility model and the change or other equivalent replacement modes of doing all contain within the scope of protection of the utility model.
Claims (4)
1. A TIRF lighting system, comprising: a light source unit and a prism;
the prism is arranged right below the light source unit, one side of the prism is a curved surface, the other side of the prism is an inclined plane with a wide upper part and a narrow lower part, and the inclined plane is positioned right below the light source unit; the observation sample that awaits measuring is placed between upper cover glass and lower cover glass, and upper cover glass is located the below of prism, fills the immersion oil between upper cover glass and the prism, and prism, immersion oil and upper cover glass's refracting index are the same, and all are greater than the refracting index of the observation sample that awaits measuring.
2. The TIRF illumination system of claim 1, wherein the light source unit comprises a laser light source, a first lens, the first lens disposed directly below the laser light source, the prism disposed directly below the first lens, and the bevel disposed directly below the first lens.
3. The TIRF illumination system of claim 1, wherein the left side of the prism is curved and the right side is a slope that is wide at the top and narrow at the bottom.
4. The TIRF lighting system of claim 1, further comprising: debugging the imaging assembly; the debugging imaging component comprises an imaging camera, a second lens and light splitting flat glass which are horizontally and sequentially arranged;
the light splitting plate glass is obliquely arranged between the lens and the inclined plane of the prism; the surface of the light-splitting plate glass facing the first lens is plated with a semi-transmission film, and the other surface of the light-splitting plate glass is plated with a total reflection transmission film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023317480.4U CN214409436U (en) | 2020-12-31 | 2020-12-31 | TIRF lighting system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023317480.4U CN214409436U (en) | 2020-12-31 | 2020-12-31 | TIRF lighting system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214409436U true CN214409436U (en) | 2021-10-15 |
Family
ID=78043488
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202023317480.4U Active CN214409436U (en) | 2020-12-31 | 2020-12-31 | TIRF lighting system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214409436U (en) |
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2020
- 2020-12-31 CN CN202023317480.4U patent/CN214409436U/en active Active
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| TR01 | Transfer of patent right |
Effective date of registration: 20230511 Address after: No. 388, Lianyun Road, Huangpu District, Guangzhou, Guangdong 510530 Patentee after: Huangpu Material Research Institute Dawan District Guangdong Hong Kong and Macao Patentee after: Wang Hongda Patentee after: Sun Jiayin Address before: No. 388, Lianyun Road, Huangpu District, Guangzhou, Guangdong 510530 Patentee before: Huangpu Material Research Institute Dawan District Guangdong Hong Kong and Macao |
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| TR01 | Transfer of patent right | ||
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Effective date of registration: 20230619 Address after: No. 388, Lianyun Road, Huangpu District, Guangzhou, Guangdong 510530 Patentee after: Huangpu Material Research Institute Dawan District Guangdong Hong Kong and Macao Patentee after: Wang Hongda Address before: No. 388, Lianyun Road, Huangpu District, Guangzhou, Guangdong 510530 Patentee before: Huangpu Material Research Institute Dawan District Guangdong Hong Kong and Macao Patentee before: Wang Hongda Patentee before: Sun Jiayin |
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| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20231103 Address after: No. 388, Lianyun Road, Huangpu District, Guangzhou, Guangdong 510530 Patentee after: Guangzhou Microvision Optical Technology Co.,Ltd. Address before: No. 388, Lianyun Road, Huangpu District, Guangzhou, Guangdong 510530 Patentee before: Huangpu Material Research Institute Dawan District Guangdong Hong Kong and Macao Patentee before: Wang Hongda |