CN214361395U - Polymerase chain reaction amplification device - Google Patents
Polymerase chain reaction amplification device Download PDFInfo
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- CN214361395U CN214361395U CN202022288487.1U CN202022288487U CN214361395U CN 214361395 U CN214361395 U CN 214361395U CN 202022288487 U CN202022288487 U CN 202022288487U CN 214361395 U CN214361395 U CN 214361395U
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Abstract
The utility model relates to a polymerase chain reaction amplification device, which comprises a chip bearing component, a chip detection component and a chip amplification component, wherein the chip bearing component is used for bearing a microfluidic chip so that the microfluidic chip provides a required environment for polymerase chain reaction; the temperature adjusting assembly is jointed with the chip bearing assembly and controls the temperature of the microfluidic chip by adjusting the temperature of the chip bearing assembly; and the control module comprises a logic control circuit connected with the temperature adjusting component and a timer circuit connected with the logic control circuit, wherein the logic control circuit can control the temperature adjusting component and control the temperature of the microfluidic chip by using the temperature adjusting component, and the timer circuit can automatically restart the logic control circuit and enable the logic control circuit to be continuously controlled by the shutdown. The polymerase chain reaction amplification device can reduce the influence of the polymerase chain reaction amplification device on an experiment after downtime, and reduce the waste of polymerase chain reaction raw materials, time and cost.
Description
Technical Field
The utility model belongs to the technical field of polymerase chain reaction amplifications, concretely relates to polymerase chain reaction amplification device.
Background
The Polymerase Chain Reaction (PCR) amplification device is a device for amplifying specific DNA, and has the characteristics of strong specificity, high sensitivity and high speed, so that it is widely used in medical and biological laboratories, and can be used for determining whether a sample will show a map of a certain genetic disease, diagnosis of infectious diseases, gene replication and paternity test.
The existing polymerase chain reaction amplification device mainly comprises a chip bearing component for bearing a microfluidic chip, a temperature regulating component for regulating the temperature of the microfluidic chip and a control module for controlling the temperature regulating component. The inventor of the application finds out after creative work that although the control module can control the temperature adjusting assembly through a preset control rule and control the temperature of the microfluidic chip by using the temperature adjusting assembly, if the control module goes down, the temperature adjusting assembly cannot be controlled to complete temperature rise and temperature fall, the temperature change rule of polymerase chain reaction is directly damaged, the PCR amplification test fails, the test needs to be carried out again, and the raw materials, time and cost of the polymerase chain reaction are greatly wasted.
SUMMERY OF THE UTILITY MODEL
In order to solve the above-mentioned whole or partial problem, the utility model aims to provide a polymerase chain reaction amplification device, it can reduce the influence of polymerase chain reaction amplification device to the experiment after taking place to shut down, reduces the waste to polymerase chain reaction raw and other materials, time and cost.
The utility model provides a polymerase chain reaction amplification device, which comprises a chip bearing component, a chip detection component and a chip amplification component, wherein the chip bearing component is used for bearing a microfluidic chip so that the microfluidic chip provides a required environment for polymerase chain reaction; the temperature adjusting assembly is jointed with the chip bearing assembly and controls the temperature of the microfluidic chip by adjusting the temperature of the chip bearing assembly; and the control module comprises a logic control circuit connected with the temperature adjusting component and a timer circuit connected with the logic control circuit, wherein the logic control circuit can control the temperature adjusting component and utilize the temperature adjusting component to control the temperature of the microfluidic chip, and the timer circuit can automatically restart the logic control circuit and enable the logic control circuit to continue to be controlled by the shutdown.
Further, the PCR amplification device comprises a memory which is connected with the logic control circuit and can store data required by the control circuit in real time.
Further, the memory is a ferroelectric data memory.
Further, the temperature adjusting assembly comprises a plate-type thermoelectric refrigerator, a heating end of the plate-type thermoelectric refrigerator is connected with the chip bearing assembly and used for increasing the temperature of the microfluidic chip, and a temperature sensor is arranged between the heating end of the plate-type thermoelectric refrigerator and the chip bearing assembly, and the control module is connected with the temperature sensor and the plate-type thermoelectric refrigerator and can control the starting, the closing and the power of the plate-type thermoelectric refrigerator according to the detection result of the temperature sensor.
Further, the chip carrier assembly includes a heat transfer sheet provided on the plate-type thermoelectric refrigerator and used for carrying the microfluidic chip, wherein the temperature sensor is inserted into the heat transfer sheet from a side surface thereof.
Further, the temperature adjusting assembly further comprises a heat dissipation fin connected with the refrigerating end of the plate-type thermoelectric refrigerator and a fan capable of driving airflow to flow through the heat dissipation fin.
Further, polymerase chain reaction amplification device still includes the casing that has fresh air inlet and fresh air inlet, chip carrier assembly establishes on the top of casing, plate-type thermoelectric refrigerator, heat dissipation fin and fan are established range upon range of in the casing, and make plate-type thermoelectric refrigerator with chip carrier assembly's heat transfer lamellar body supports and leans on.
Further, the pcr amplification apparatus further comprises a display screen disposed on the top of the housing and connected to the control module, and/or a speaker disposed in the housing and connected to the control module.
Furthermore, the chip bearing assembly further comprises a heat insulation substrate with a hollow part and a buckle cover capable of being buckled on the heat insulation substrate, and the heat transfer sheet body is arranged in the hollow part and can bear the microfluidic chip in the hollow part.
Further, the temperature sensor is a thermistor or a thermocouple.
According to the above technical scheme, the utility model provides a polymerase chain reaction amplification device passes through control module's logic control circuit control temperature regulation subassembly and controls the temperature to micro-fluidic chip, in order to reduce because of the produced adverse effect of machine downtime on this basis, this polymerase chain reaction amplification device has still add the timer circuit in control module, and rely on the timer circuit automatic heavy logic control circuit when the machine downtime appears in the logic control circuit and make its control before the machine downtime that continues, can effectively reduce the influence to the experiment after the machine downtime takes place in polymerase chain reaction amplification device like this, reduce the raw and other materials of polymerase chain reaction, the waste of time and cost. And simultaneously, the utility model provides a polymerase chain reaction amplification device's simple structure, assembly are easy, use safe and reliable, and the implementation of being convenient for is popularized and applied.
Drawings
Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. In the figure:
FIG. 1 is a functional block diagram of a polymerase chain reaction amplification apparatus according to an embodiment of the present invention;
FIG. 2 is an exploded view of a PCR amplification apparatus according to an embodiment of the present invention;
FIG. 3 is an exploded view of a temperature control module and a chip carrier module of a PCR amplification apparatus according to an embodiment of the present invention.
In the drawings, like parts are provided with like reference numerals. The figures are not drawn to scale.
Detailed Description
The present invention will be further explained with reference to the accompanying drawings.
Fig. 1 is a functional block diagram of a pcr amplification apparatus according to an embodiment of the present invention, fig. 2 is an exploded view of the pcr amplification apparatus according to an embodiment of the present invention, and fig. 3 is an exploded view of a temperature adjustment module and a chip carrier module of the pcr amplification apparatus according to an embodiment of the present invention. As shown in fig. 1, 2 and 3, the pcr amplification apparatus 100 includes a chip carrier assembly 40, a temperature adjustment assembly 10 and a control module 20. The chip carrier assembly 40 is used for carrying a microfluidic chip, so that the microfluidic chip provides a required environment for polymerase chain reaction. The temperature regulating assembly 10 is engaged with the chip carrier assembly 40 and controls the temperature of the microfluidic chip by adjusting the temperature of the chip carrier assembly 40. The control module is preferably a CPU or a motherboard containing a CPU. The control module comprises a logic control circuit connected with the temperature adjusting component and a timer circuit connected with the logic control circuit. The logic control circuit can control the temperature regulating component through a preset control rule (such as a known PID algorithm) and control the temperature of the microfluidic chip by using the temperature regulating component, and the timer circuit can automatically restart the logic control circuit when the shutdown occurs and enable the logic control circuit to continuously execute the control rule interrupted by the shutdown.
That is to say, the utility model discloses polymerase chain reaction amplification device can control the temperature through control module's logic control circuit control temperature regulating assembly to micro-fluidic chip, on this basis in order to reduce because of the produced adverse effect of machine of dying, this polymerase chain reaction amplification device has still add the timer circuit in control module, and rely on the timer circuit to automatic heavy logic control circuit when the machine appears dying in logic control circuit and make its control before the machine that lasts, can effectively reduce the influence to the experiment after polymerase chain reaction amplification device takes place to dying like this, reduce the waste to polymerase chain reaction raw and other materials, time and cost.
In this embodiment, the pcr amplification apparatus 100 further includes a memory 23 connected to the logic control circuit 21 and capable of storing data required for control thereof in real time. In use, the memory 23 is mainly used for storing functions, variables and constants in the predetermined control rules in real time to ensure that the logic control circuit continues the control rules before downtime, especially the variables before downtime. It is noted that ferroelectric data memories have a very long lifetime and a high reliability, so that the choice of ferroelectric data memories is suggested as a preferred embodiment of the memory.
In this embodiment, the temperature adjustment assembly 10 includes a plate-type thermoelectric cooler 11 engaged with the chip carrier assembly 40 and used to increase the temperature of the microfluidic chip. The heat generating end of the plate-type thermoelectric refrigerator 11 is joined with the chip carrier assembly 40 and is used for increasing the temperature of the microfluidic chip. The temperature adjustment assembly 10 further includes a temperature sensor 12 disposed between the heat generating end of the plate-type thermoelectric refrigerator 11 and the chip carrier assembly 40. The temperature sensor 12 may be selected as a thermocouple or a thermistor. Preferably, a Wheatstone bridge thermometry circuit may be built into the control module 20 with the aid of thermistors and will be connected to a logic control circuit to improve the accuracy of the temperature measurement. The control module 20 is connected with the temperature sensor 12 and the plate-type thermoelectric refrigerator 11, and can control the starting, the closing and the power of the plate-type thermoelectric refrigerator 11 according to the detection result of the temperature sensor 12. In this way, the control module 20 can automatically control the thermoelectric refrigerator 11 to better meet the heat requirement of the microfluidic chip.
In this embodiment, the chip carrier assembly 40 further includes a heat transfer sheet 41 disposed on the plate-type thermoelectric refrigerator 11, and the heat transfer sheet 41 is used for carrying the microfluidic chip. The heat transfer sheet 41 is used for uniformly transferring heat at the heat generating end of the plate-type thermoelectric refrigerator 11 to the microfluidic chip. Preferably, the material of the heat transfer sheet 41 is copper, because copper has the advantages of corrosion resistance and good thermal conductivity. The temperature sensor 12 is inserted into the heat transfer sheet 41, and can accurately monitor the temperature of the microfluidic chip.
The temperature adjusting assembly 10 may further include a heat dissipating fin 13 coupled to the cooling end of the plate-type thermoelectric refrigerator 11 and a fan 14 capable of driving an air flow to flow through the heat dissipating fin 13, so as to reduce the influence of the cooling end of the plate-type thermoelectric refrigerator 11 on the heating end during the cooling process and improve the heating effect of the plate-type thermoelectric refrigerator 11 on the microfluidic chip indirectly.
In this embodiment, the pcr amplification apparatus 100 further includes a housing 50 having an air inlet hole and an air outlet hole. The housing 50 serves to provide protection for the chip carrier assembly 40, the temperature regulating assembly 10 and the control module 20 and to make them easy to store. The chip carrier assembly 40 is disposed on the top of the casing 50, and the plate-type thermoelectric cooler 11, the heat dissipation fins 13 and the fan 14 are stacked in the casing 50, so that the plate-type thermoelectric cooler 11 abuts against the heat transfer sheet 41 of the chip carrier assembly 40, thereby effectively utilizing the internal space of the casing 50.
In this embodiment, the pcr amplification apparatus 100 further includes a display 30 disposed on the top of the housing 50 and connected to the control module 20, and/or a speaker disposed in the housing 50 and connected to the control module 20. The display 30 is used for displaying the temperature value detected by the temperature sensor 12 and information such as the reaction program, the working time and the like of the pcr amplification device 100, and the speaker is used for playing a warning tone after the reaction is started, the reaction is completed or the downtime occurs.
In this embodiment, the chip carrier assembly 40 includes a heat insulation substrate 42 having a hollow portion and a cover 43 capable of being fastened on the heat insulation substrate 42, and the heat transfer sheet 41 is disposed in the hollow portion and capable of carrying the micro-fluidic chip in the hollow portion. It is preferable to use phenolic foam as the material of the thermal insulation substrate 42 because it has better flame resistance and higher thermal insulation, and the purpose of adding the cover 43 is to make the temperature around the microfluidic chip uniform and improve the working efficiency of the temperature adjustment assembly 10. Meanwhile, the hollow-out part of the heat insulation substrate 42 fixes the position of the micro-fluidic chip, and the reduction of heat exchange efficiency caused by the relative movement between the micro-fluidic chip and the heat transfer sheet 41 in the working process is avoided.
According to the above technical scheme, the utility model provides a polymerase chain reaction amplification device 100 can reduce the influence of polymerase chain reaction amplification device to the experiment after taking place to shut down, reduces the waste to polymerase chain reaction raw and other materials, time and cost.
Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.
In this application, unless expressly stated or limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can include, for example, fixed connections, removable connections, or integral parts; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
The above description is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily make changes or variations within the technical scope of the present invention, and such changes or variations should be covered by the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims. The technical features mentioned in the embodiments can be combined in any way as long as there is no structural conflict. The present invention is not limited to the particular embodiments disclosed herein, but encompasses all technical solutions falling within the scope of the claims.
Claims (10)
1. A polymerase chain reaction amplification apparatus, comprising:
the chip bearing assembly is used for bearing the microfluidic chip so that the microfluidic chip provides a required environment for polymerase chain reaction;
the temperature adjusting assembly is jointed with the chip bearing assembly and controls the temperature of the microfluidic chip by adjusting the temperature of the chip bearing assembly;
and the control module comprises a logic control circuit connected with the temperature adjusting component and a timer circuit connected with the logic control circuit, wherein the logic control circuit can control the temperature adjusting component and utilize the temperature adjusting component to control the temperature of the microfluidic chip, and the timer circuit can automatically restart the logic control circuit and enable the logic control circuit to continue to be controlled by the shutdown.
2. The pcr amplification apparatus as claimed in claim 1, wherein the pcr amplification apparatus comprises a memory connected to the logic control circuit and capable of storing data required for controlling the pcr amplification apparatus in real time.
3. The pcr amplification apparatus as claimed in claim 2, wherein the memory is a ferroelectric data memory.
4. The pcr amplification apparatus as claimed in any one of claims 1 to 3, wherein the temperature adjustment assembly comprises a plate-type thermoelectric refrigerator having a heat generating end engaged with the chip carrier assembly and configured to increase the temperature of the microfluidic chip, and a temperature sensor disposed between the heat generating end of the plate-type thermoelectric refrigerator and the chip carrier assembly, and the control module is connected to the temperature sensor and the plate-type thermoelectric refrigerator and configured to control the start, the stop, and the power of the plate-type thermoelectric refrigerator according to the detection result of the temperature sensor.
5. The pcr amplification apparatus of claim 4, wherein the chip carrier assembly comprises a heat transfer sheet provided on the plate-type thermoelectric refrigerator and configured to carry the microfluidic chip, wherein the temperature sensor is inserted into the heat transfer sheet from a side thereof.
6. The pcr amplification apparatus of claim 5, wherein the temperature adjustment assembly further comprises a heat sink fin coupled to the cooling end of the plate-type thermoelectric cooler and a fan capable of driving an air flow through the heat sink fin.
7. The pcr amplification apparatus of claim 6, further comprising a housing having air inlet holes and air outlet holes, wherein the chip carrier assembly is disposed on a top portion of the housing, and the plate-type thermoelectric cooler, the heat dissipation fins and the fan are disposed in the housing in a stacked manner such that the plate-type thermoelectric cooler abuts against the heat transfer sheet of the chip carrier assembly.
8. The pcr amplification apparatus of claim 7, further comprising a display screen disposed on a top of the housing and connected to the control module, and/or a speaker disposed within the housing and connected to the control module.
9. The PCR amplification apparatus of claim 5, wherein the chip carrier assembly further comprises a heat-insulating substrate having a hollow portion and a cover capable of being fastened to the heat-insulating substrate, and the heat-transfer sheet is disposed in the hollow portion and capable of carrying the microfluidic chip in the hollow portion.
10. The PCR amplification apparatus of claim 4, wherein the temperature sensor is a thermistor or a thermocouple.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022288487.1U CN214361395U (en) | 2020-10-14 | 2020-10-14 | Polymerase chain reaction amplification device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022288487.1U CN214361395U (en) | 2020-10-14 | 2020-10-14 | Polymerase chain reaction amplification device |
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| Publication Number | Publication Date |
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| CN214361395U true CN214361395U (en) | 2021-10-08 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202022288487.1U Active CN214361395U (en) | 2020-10-14 | 2020-10-14 | Polymerase chain reaction amplification device |
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| CN (1) | CN214361395U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112280670A (en) * | 2020-10-14 | 2021-01-29 | 北京普若博升生物科技有限公司 | Polymerase chain reaction amplification device |
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2020
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112280670A (en) * | 2020-10-14 | 2021-01-29 | 北京普若博升生物科技有限公司 | Polymerase chain reaction amplification device |
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| TR01 | Transfer of patent right |
Effective date of registration: 20220223 Address after: 100176 room 510, unit 3, floor 4, building 4, No. 5 and No. 5 courtyard, Jinxing West Road, Daxing District, Beijing Patentee after: Beijing yuanjingtaike Biotechnology Co.,Ltd. Address before: 101102 201, 2 / F, building 14, No.15, Jingsheng south 2nd Street, Tongzhou District, Beijing Patentee before: BEIJING PUNUO BOSHENG BIOTECHNOLOGY CO.,LTD. |
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