CN214344584U - Microfluid device for processing crude product of cyclic polypeptide - Google Patents
Microfluid device for processing crude product of cyclic polypeptide Download PDFInfo
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- CN214344584U CN214344584U CN202023314314.9U CN202023314314U CN214344584U CN 214344584 U CN214344584 U CN 214344584U CN 202023314314 U CN202023314314 U CN 202023314314U CN 214344584 U CN214344584 U CN 214344584U
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Abstract
The utility model relates to a microfluid device for containing ring polypeptide crude is handled, including polypeptide crude storage tank (1), capillary column (2), mobile phase storage tank (3), peristaltic pump (4), connect appearance device, polypeptide crude storage tank (1) all is connected with capillary column (2) through the pipeline with mobile phase storage tank (3), the lower extreme of capillary column (2) is provided with the sample export, the sample export is connected with peristaltic pump (4) through the pipeline, peristaltic pump (4) with connect appearance device to be connected, peristaltic pump (4) are connected with automatic control device (5) electricity. The utility model discloses a mode of capillary carries out the column chromatography separation to the polypeptide, and the polypeptide sample evenly flows out with the form of cyclic annular aspect in the capillary to increase the resolution, set up a plurality of capillaries, thereby increased the efficiency that microfluid device handled the polypeptide. The peristaltic pump is arranged, the separation time is shortened, and a set of sample receiving equipment is adopted to receive all capillaries in the device, so that the efficiency and the quality of the treatment of the crude polypeptide product are ensured.
Description
Technical Field
The utility model relates to a cyclic polypeptide production facility technical field, concretely relates to a microfluid device for containing cyclic polypeptide crude processing.
Background
Cyclic polypeptides, polymers formed by covalent linkage of two or more amino groups through peptide bonds. Are compounds in which amino acids are linked by peptide bonds, and the products of incomplete hydrolysis of proteins are also peptides. Peptides are called dipeptides, tripeptides, tetrapeptides, etc. respectively, depending on the number of amino acids constituting the peptide, which are different from 2, 3, and 4, etc., and generally contain less than 10 amino acids called oligopeptides (oligopeptides), and polypeptides composed of more than 10 amino acids called polypeptides, which are simply referred to as peptides. Amino acids in peptide chains are not free amino acid molecules, since both the amino and carboxyl groups are bound away in the formation of peptide bonds, and thus amino acids in both polypeptide and protein molecules are referred to as > amino acid residues (amino acid residues).
Microfluidics refers to the technology of controlling, manipulating and detecting complex fluids at microscopic dimensions, and is a new and new interdisciplinary developed on the basis of microelectronics, micromachines, bioengineering and nanotechnology. In scientific experiments of biology, chemistry, materials and the like, operations on fluid are often required, such as preparation of sample DNA, liquid chromatography, PCR reaction, electrophoresis detection and the like are all performed in a liquid phase environment. If the steps of sample preparation, biochemical reaction, result detection, etc. are integrated on a biochip, the amount of fluid used for the experiment is reduced from milliliter, micro-liter to nanoliter or picoliter, and a powerful microfluidic device is necessary. Therefore, with the development of biochip technology, microfluidics technology has gained more and more attention as a key supporting technology of biochip.
In the prior art, the obtained cyclic polypeptide crude product needs to be purified to obtain the cyclic polypeptide with higher purity, but the separation degree of the existing purification equipment is not high enough, impurities are removed, and some cyclic polypeptides are also taken away, so that a microfluid device for processing the cyclic polypeptide crude product needs to be designed, the polycyclic polypeptide is purified by adopting the microfluid technology, and the waste of the cyclic polypeptide is reduced while the purity of the cyclic polypeptide is improved.
SUMMERY OF THE UTILITY MODEL
In order to solve the above problems, the utility model discloses
The utility model provides a microfluid device for containing cyclic polypeptide crude processing, includes polypeptide crude storage tank (1), capillary column (2), mobile phase storage tank (3), peristaltic pump (4), connects a kind device, polypeptide crude storage tank (1) all be connected with capillary column (2) through the pipeline with mobile phase storage tank (3), the lower extreme of capillary column (2) be provided with the sample outlet, the sample outlet pass through the pipeline and be connected with peristaltic pump (4), peristaltic pump (4) with connect a kind device and be connected, peristaltic pump (4) are connected with automatic control device (5) electricity.
Further, connect appearance device include micromotor (6), spring fastener (7), waste liquid jar (8), sample jar (9), the pivot of micromotor (6) be connected with spring fastener (7) through connecting rod (10), the front end card of the appearance pipeline of being connected with peristaltic pump (4) is on spring fastener (7), the inlet pipe of waste liquid jar (8) and the inlet pipe of sample jar (9) all set up the below at spring fastener (7), micromotor (6) be connected with automatic control device (5) electricity.
Further, the spring clamping piece (7) comprises a fixing block (701), a compression spring (702) and a clamping groove (703), the fixing block (701) is installed on the connecting rod (10), a through hole (704) is formed in the middle of the fixing block (701), the compression spring (702) is fixed inside the through hole (704), the clamping groove (703) is installed at one end of the compression spring (702), and a pipeline is clamped between the clamping groove (702) and the inner wall of the through hole (704).
Further, the polypeptide crude product storage tank (1) is simultaneously connected with a plurality of capillary columns (2) through pipelines, the mobile phase storage tank (3) is simultaneously connected with a plurality of capillary columns (2) through pipelines, resin fillers (201) are filled in the capillary columns (2), each pipeline is provided with an electric switch valve (11), and the electric switch valves (11) are electrically connected with the automatic control device (5).
Under the control of the automatic control device, the flowing time of the polypeptide is set through a plurality of tests, so that the rotation of the micromotor is driven at the set time point, the outlet pipeline is rotated right above the inlet of the inlet pipeline of the sample tank at the time point when the polypeptide comes out, the polypeptide is separated, and impurities flow into the waste liquid tank.
The utility model has the advantages that: the utility model provides a microfluid device is provided with a plurality of tubules that are filled with resin material, the cross-sectional area of tubule is less than the cross-sectional area of normal detached column far away, thereby the speed of flowing at the polypeptide sample in the pipe is the same, and very sectional area of general detached column is big, the polypeptide sample can not evenly flow with the mode of an annular aspect in the post, thereby it is little to lead to the resolution, consequently, the mode that adopts the capillary carries out the column chromatography separation to the polypeptide, the polypeptide sample evenly flows with the form of annular aspect in the capillary, thereby increase the resolution, set up a plurality of capillaries, thereby the efficiency that microfluid device handled the polypeptide has been increased. And the peristaltic pump is arranged in the microfluid device and provides power for the separation of the polypeptide in the capillary, thereby shortening the separation time, and the sample receiving of all the capillaries in the device can be completed by adopting a set of sample receiving equipment, thereby ensuring the efficiency and quality of the treatment of crude polypeptide.
Drawings
FIG. 1 is a schematic diagram of a microfluidic device for processing crude cyclic polypeptide according to an embodiment of the present invention.
Fig. 2 is a schematic diagram of a spring clip of a microfluidic device for processing crude cyclic polypeptide according to an embodiment of the present invention.
Fig. 3 is a control flow diagram of a microfluidic device for processing crude cyclic polypeptide according to an embodiment of the present invention.
List of reference numerals: 1-a polypeptide crude product storage tank, 2-a capillary column, 201-a resin filler, 3-a mobile phase storage tank, 4-a peristaltic pump, 5-an automatic control device, 6-a micromotor, 7-a spring clamping piece, 701-a fixed block, 702-a compression spring, 703-a clamping groove, 704-a through hole, 8-a waste liquid tank, 9-a sample tank, 10-a connecting rod and 11-an electric switch valve.
Detailed Description
The present invention will be further explained with reference to the accompanying drawings and embodiments, which are to be understood as illustrative only and not limiting the scope of the invention. It should be noted that the terms "front," "back," "left," "right," "upper" and "lower" used in the following description refer to directions in the drawings, and the terms "inner" and "outer" refer to directions toward and away from, respectively, the geometric center of a particular component.
A microfluid device for processing a crude polypeptide containing a ring comprises a crude polypeptide storage tank 1, a capillary column 2, a mobile phase storage tank 3, a peristaltic pump 4 and a sample receiving device, wherein the crude polypeptide storage tank 1 and the mobile phase storage tank 3 are both connected with the capillary column 2 through pipelines, the lower end of the capillary column 2 is provided with a sample outlet, the sample outlet is connected with the peristaltic pump 4 through a pipeline, the peristaltic pump 4 is connected with the sample receiving device, and the peristaltic pump 4 is electrically connected with an automatic control device 5.
Further, connect appearance device to include micromotor 6, spring fastener 7, waste liquid jar 8, sample jar 9, micromotor 6's pivot is connected with spring fastener 7 through connecting rod 10, and the front end card of the appearance pipeline of being connected with peristaltic pump 4 is on spring fastener 7, and the inlet pipeline of waste liquid jar 8 and the inlet pipeline of sample jar 9 all set up in the below of spring fastener 7, and micromotor 6 is connected with automatic control device 5 electricity.
Further, the spring fastener 7 includes a fixing block 701, a compression spring 702 and a clamping groove 703, the fixing block 701 is installed on the connecting rod 10, a through hole 704 is formed in the middle of the fixing block 701, the compression spring 702 is fixed inside the through hole 704, and the clamping groove 703 is installed at one end of the compression spring 702 to clamp the pipeline between the clamping groove 702 and the inner wall of the through hole 704.
Further, the polypeptide crude product storage tank 1 is simultaneously connected with the plurality of capillary columns 2 through pipelines, the mobile phase storage tank 3 is simultaneously connected with the plurality of capillary columns 2 through pipelines, the interior of each capillary column 2 is filled with resin packing 201, each pipeline is provided with an electric switch valve 11, and the electric switch valves 11 are electrically connected with the automatic control device 5.
Under the control of the automatic control device, the flowing time of the polypeptide is set through a plurality of tests, so that the rotation of the micromotor is driven at the set time point, the outlet pipeline is rotated right above the inlet of the inlet pipeline of the sample tank at the time point when the polypeptide comes out, the polypeptide is separated, and impurities flow into the waste liquid tank.
The technical means disclosed by the scheme of the present invention is not limited to the technical means disclosed by the above embodiments, but also includes the technical scheme formed by the arbitrary combination of the above technical features.
Claims (4)
1. The utility model provides a microfluid device for containing cyclic polypeptide crude processing, its characterized in that includes polypeptide crude storage tank (1), capillary column (2), mobile phase storage tank (3), peristaltic pump (4), connects a kind device, polypeptide crude storage tank (1) all be connected with capillary column (2) through the pipeline with mobile phase storage tank (3), the lower extreme of capillary column (2) be provided with the sample outlet, the sample outlet pass through the pipeline and be connected with peristaltic pump (4), peristaltic pump (4) with connect a kind device and be connected, peristaltic pump (4) are connected with automatic control device (5) electricity.
2. The microfluid device for crude cyclopeptide processing according to claim 1, wherein the sample receiving device comprises a micromotor (6), a spring clamp (7), a waste liquid tank (8), and a sample tank (9), the rotating shaft of the micromotor (6) is connected with the spring clamp (7) through a connecting rod (10), the front end of a sample outlet pipe connected with the peristaltic pump (4) is clamped on the spring clamp (7), the inlet pipe of the waste liquid tank (8) and the inlet pipe of the sample tank (9) are both arranged below the spring clamp (7), and the micromotor (6) is electrically connected with the automatic control device (5).
3. The microfluid device for crude processing of loop-containing polypeptides of claim 2, wherein the spring clip (7) comprises a fixing block (701), a compression spring (702) and a clamping groove (703), the fixing block (701) is mounted on the connecting rod (10), a through hole (704) is formed in the middle of the fixing block (701), the compression spring (702) is fixed inside the through hole (704), and the clamping groove (703) is mounted at one end of the compression spring (702) to clamp the pipeline between the clamping groove (703) and the inner wall of the through hole (704).
4. The microfluidic device for crude cyclic polypeptide treatment according to claim 1, wherein the crude cyclic polypeptide tank (1) is connected to a plurality of capillary columns (2) through a pipeline at the same time, the mobile phase tank (3) is connected to a plurality of capillary columns (2) through a pipeline at the same time, the capillary columns (2) are filled with resin packing (201), each pipeline is provided with an electrically-operated switch valve (11), and the electrically-operated switch valves (11) are electrically connected to the automatic control device (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023314314.9U CN214344584U (en) | 2020-12-31 | 2020-12-31 | Microfluid device for processing crude product of cyclic polypeptide |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023314314.9U CN214344584U (en) | 2020-12-31 | 2020-12-31 | Microfluid device for processing crude product of cyclic polypeptide |
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| Publication Number | Publication Date |
|---|---|
| CN214344584U true CN214344584U (en) | 2021-10-08 |
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| CN202023314314.9U Active CN214344584U (en) | 2020-12-31 | 2020-12-31 | Microfluid device for processing crude product of cyclic polypeptide |
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