CN214209709U

CN214209709U - Stem cell filtering and separating device

Info

Publication number: CN214209709U
Application number: CN202022594296.8U
Authority: CN
Inventors: 杨敏
Original assignee: Nanchang Longlaifu Biotechnology Co ltd
Current assignee: Nanchang Longlaifu Biotechnology Co ltd
Priority date: 2020-11-11
Filing date: 2020-11-11
Publication date: 2021-09-17
Anticipated expiration: 2030-11-11

Abstract

The utility model relates to the technical field of cell separation, and discloses a stem cell filtering and separating device, which comprises a bottle body, the top of the bottle body is movably provided with a bottle cap, the periphery of the top of the bottle body is fixedly provided with a convex block, the two sides of the convex block are rotatably provided with a rotating block, the top of the rotating block penetrates through the convex block and extends to the outside of the convex block, the top of the rotating block is fixedly provided with a pressing block, the bottom of the rotating block is fixedly provided with a pressed block, the inner surfaces of the rotating block and the pressing block are movably connected with the outer surface of the bottle cap, the stem cell filtering and separating device presses the pressed block through the bottom of the bottle cap to ensure that the pressing block presses the bottle cap and a sealing adhesive layer in the bottle cap tightly clings to the bottleneck of a separating bottle, so that the bottle body and the bottle cap are tightly fixed, the effective sealing of the inner part of the bottle body is realized, and the separating bottle can not be influenced by external factors when in use, so that the inner cell sap can not be polluted by external bacteria.

Description

Stem cell filtering and separating device

Technical Field

The utility model relates to a cell separation technical field specifically is a stem cell filters separator.

Background

Stem cells are a kind of cells with unlimited or immortal self-renewal capacity, capable of producing at least one kind of highly differentiated progeny cells, and most biologists and physicians currently consider stem cells to be a kind of cells from embryonic, fetal or adult bodies with unlimited self-renewal and proliferative differentiation capacity under certain conditions, capable of producing daughter cells with completely identical phenotype and genotype as well as self, capable of producing specialized cells constituting body tissues and organs, and capable of differentiating into progenitor cells, and the currently common stem cell sorting methods are: immunomagnetic bead sorting, density gradient sorting, whole bone marrow adherence, and tissue digestion.

When the existing stem cell filtering and separating device is used, the inner and outer sealing performance of the bottle body is not high, so that when the separating device is used for separating stem cells, cell sap is easily polluted by external bacteria, the purity of the separated stem cells is low, the practicability is low, the permanent magnet is not easy to mount and take down, the action area of the permanent magnet is small, the stem cell separating efficiency is low, and the stem cells are not easy to take out.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

Not enough to prior art, the utility model provides a stem cell filtering separation device possesses the inside and outside seal of bottle, and the simple and easy installation of permanent magnet and the advantage of taking off have solved bottle separation stem cell time closure not high, pollute solution in the bottle easily, and the permanent magnet is not installed and is taken off well, the less problem of permanent magnet area of action.

(II) technical scheme

In order to solve the technical problem, the utility model provides a following technical scheme: the utility model provides a stem cell filters separator, includes the bottle, the top movable mounting of bottle has the bottle lid, equal fixed mounting all around at bottle top has the lug, the both sides of lug are all rotated and are installed the turning block, the top of turning block is run through the lug and is extended to the outside of lug, the top fixed mounting of turning block has the press block, the bottom fixed mounting of turning block has the pressure piece, the turning block with press the internal surface of pressing the piece all with the surface swing joint of bottle lid, the top of pressure piece and the bottom swing joint of bottle lid, the joint groove has been seted up at the top of bottle, the internal surface in joint groove and the surface swing joint of bottle lid, the inner chamber fixed mounting of bottle lid has the closed glue film.

Preferably, the inner cavity of the bottle body is movably provided with a separation bottle, and both sides of the separation bottle are provided with filter holes in a penetrating manner.

Preferably, the magnet fixing device is movably installed around the bottle body and comprises a fixing ring, and a permanent magnet is fixedly installed at the bottom of the fixing ring.

Preferably, the equal slidable mounting in both sides at solid fixed ring top has the piece of drawing, two draw the equal fixed mounting in one side that the piece is relative to have the fixed block, the fixed slot has all been seted up to the both sides of bottle, two fixed block relative one side all runs through solid fixed ring and extends to solid fixed ring's outside, the surface of fixed block and the internal surface sliding connection of fixed slot.

Preferably, the equal fixed mounting in both sides of solid fixed ring inner chamber has the spring, two one side that the fixed block is relative all runs through solid fixed ring and extends to solid fixed ring's outside, the surface of fixed block overlaps with the internal surface of spring and establishes, two one side that the spring is relative all is connected with the external fixed surface of two fixed blocks.

Preferably, the bottom fixed mounting who draws the piece has the stopper, the both sides of solid fixed ring just are located the below of drawing the piece and have all seted up the spacing groove, the surface of stopper and the internal surface sliding connection in spacing groove.

(III) advantageous effects

Compared with the prior art, the utility model provides a stem cell filtering separation device possesses following beneficial effect: the stem cell filtering and separating device can pour cell sap into a separating bottle, the separating bottle is placed into a bottle body, a bottle cover is covered on the bottle body along a clamping groove, a pressure block is contacted with the pressure block, the pressure block is enabled to compress the bottle cover, meanwhile, a glue sealing layer is sealed to compress the bottleneck of the separating bottle, a pulling block is pulled towards two sides, a magnet fixing device is sleeved on the bottle body, when the fixing groove is reached, a fixing block is pressed into the fixing groove to fix the magnet fixing device, the separating device is placed in a standing mode, marked stem cells are filtered by a permanent magnet through a filtering hole and flow into the bottle body, then the pressure block is pulled towards two sides, the bottle cover is upwards pushed out of the clamping groove by the pressure block, the separating bottle is taken out of the bottle body, the pulling block is pulled towards two sides to enable the fixing block to leave the fixing groove, the magnet fixing device is taken out of the bottle body, and the bottom of the bottle body is the filtered stem cells needing to be separated.

1. This stem cell filtering separation device will be pressed by the pressure piece through bottle lid bottom, make and press the pressure piece and compress tightly the bottle lid, and make the bottleneck that the closed glue film in the bottle lid pasted tight separation bottle, make bottle and bottle lid closely fixed, realized effectively sealing to the bottle inside, the separation bottle can not receive external factor's influence when using, make inside cell liquid can not be polluted by external bacterium, the effective quality of the stem cell of having improved the separation, stability when having increased the splitter and using.

2. This stem cell filters separator, through solid fixed ring and fixed slot, make installation that the permanent magnet can be convenient and separate out the bottle, make separator more convenient when using, it is easier when having made to filter to draw stem cell after the stem cell, can not influenced by the permanent magnet, the ring nature permanent magnet can be all-round simultaneously to being acted on by magnetic mark stem cell in the separation bottle, improve the efficiency that filters stem cell, reduced the required time of filtration stem cell.

Drawings

Fig. 1 is a sectional view of the internal structure of the present invention.

Fig. 2 is a top view of the external structure of the present invention.

Fig. 3 is a top view of the external structure of the magnet fixing device of the present invention.

In the figure: 1. a bottle body; 2. a bottle cap; 3. a bump; 4. rotating the block; 5. a pressing block; 6. a pressed block; 7. A clamping groove; 8. sealing the adhesive layer; 9. separating the bottles; 10. a filtration pore; 11. a magnet fixing device; 12. a fixing ring; 13. a permanent magnet; 14. pulling the block; 15. a fixed block; 16. fixing grooves; 17. a spring; 18. a limiting block; 19. a limiting groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-3, a stem cell filtering and separating device comprises a bottle body 1, a bottle cap 2 is movably mounted at the top of the bottle body 1, a convex block 3 is fixedly mounted at the periphery of the top of the bottle body 1, rotating blocks 4 are rotatably mounted at both sides of the convex block 3, the top of the rotating block 4 penetrates through the convex block 3 and extends to the outside of the convex block 3, a pressing block 5 is fixedly mounted at the top of the rotating block 4, a pressed block 6 is fixedly mounted at the bottom of the rotating block 4, the inner surfaces of the rotating block 4 and the pressing block 5 are movably connected with the outer surface of the bottle cap 2, the top of the pressed block 6 is movably connected with the bottom of the bottle cap 2, a clamping groove 7 is formed at the top of the bottle body 1, the inner surface of the clamping groove 7 is movably connected with the outer surface of the bottle cap 2, a sealing glue layer 8 is fixedly mounted in the inner cavity of the bottle cap 2, a separating bottle 9 is movably mounted in the inner cavity of the bottle body 1, and filtering holes 10 are formed at both sides of the separating bottle 9, the periphery of the bottle body 1 is movably provided with a magnet fixing device 11, the magnet fixing device 11 comprises a fixing ring 12, the bottom of the fixing ring 12 is fixedly provided with a permanent magnet 13, the permanent magnet 13 is annular, two sides of the top of the fixing ring 12 are respectively provided with a pulling block 14 in a sliding manner, one side of each of the two pulling blocks 14 opposite to each other is fixedly provided with a fixing block 15, two sides of the bottle body 1 are respectively provided with a fixing groove 16, one side of each of the two fixing blocks 15 opposite to each other penetrates through the fixing ring 12 and extends to the outside of the fixing ring 12, the outer surface of each fixing block 15 is in sliding connection with the inner surface of the corresponding fixing groove 16, two sides of the inner cavity of the corresponding fixing ring 12 are respectively fixedly provided with a spring 17, one side of each fixing block 15 opposite to each fixing ring penetrates through the corresponding fixing ring 12 and extends to the outside of the corresponding fixing ring 12, the outer surface of each fixing block 15 is sleeved with the inner surface of the corresponding spring 17, and one side of each spring 17 is fixedly connected with the outer surfaces of the corresponding fixing blocks 15, the bottom of pulling block 14 is fixedly provided with a limiting block 18, the two sides of fixing ring 12 are both located below pulling block 14 and are provided with a limiting groove 19, and the outer surface of limiting block 18 is connected with the inner surface of limiting groove 19 in a sliding manner.

The working principle is as follows: firstly, a worker marks stem cells to be separated in cell sap out by using superparamagnetism micro magnetic beads specifically, the cell sap is poured into a separation bottle 9, the separation bottle 9 is placed into a bottle body 1, a bottle cap 2 is covered on the bottle body 1 along a clamping groove 7, the bottom of the bottle cap 2 contacts a pressure block 6 and rotates the pressure block 6 around a convex block 3 while moving downwards, a pressing block 5 on a rotating block 4 presses the bottle cap 2 on the bottle body 1, a sealing glue layer 8 is pressed by the bottle cap 2 to the mouth of the separation bottle 9, a fixing ring 12 is pulled upwards by a pulling block 14 to two sides, a magnet fixing device 11 is sleeved on the bottle body 1, when reaching a fixing groove 16, a fixing block 15 is pressed into the fixing groove 16 by a spring 17 to fix the magnet fixing device 11, a permanent magnet 13 is attached to the bottle body 1, the separation device is stood, the marked stem cells are filtered out of the separation bottle 9 by the permanent magnet 13 through a filtering hole 10 and flow into the bottle body 1, then the pressing block 5 is pulled towards two sides, so that the bottom of the bottle cap 2 is pushed upwards out of the clamping groove 7 by the pressure receiving block 6, the separation bottle 9 with the filtered stem cells is taken out of the bottle body 1, the pulling block 14 is pulled towards two sides, the fixing block 15 is separated from the fixing groove 16, the magnet fixing device 11 is taken out of the bottle body 1, and the stem cells to be separated and filtered are arranged at the bottom of the bottle body 1.

In conclusion, the stem cell filtering and separating device can be obtained by pouring cell sap into a separating bottle 9, placing the separating bottle 9 into a bottle body 1, covering a bottle cap 2 on the bottle body 1 along a clamping groove 7, contacting a pressure receiving block 6, enabling a pressing block 5 to press the bottle cap 2, simultaneously enabling a sealing glue layer 8 to press a bottle mouth of the separating bottle 9, pulling a pulling block 14 towards two sides, sleeving a magnet fixing device 11 on the bottle body 1, pressing a fixing block 15 into the fixing groove 16 when the fixing block reaches the fixing groove 16, fixing the magnet fixing device 11, standing the separating device, filtering marked stem cells by a permanent magnet 13 through a filtering hole 10 and flowing into the bottle body 1, then pulling the pressing block 5 towards two sides, enabling the bottle cap 2 to be pushed upwards by the pressure receiving block 6 out of the clamping groove 7, taking the separating bottle 9 out of the bottle body 1, pulling the pulling block 14 towards two sides, enabling the fixing block 15 to leave the fixing groove 16, and separating the magnet fixing device 11 from the bottle body 1, the stem cells to be separated and filtered out are arranged at the bottom of the bottle body 1.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a stem cell filters separator, includes bottle (1), the top movable mounting of bottle (1) has bottle lid (2), its characterized in that: bottle (1) top all around equal fixed mounting have lug (3), the both sides of lug (3) are all rotated and are installed turning block (4), the top of turning block (4) runs through lug (3) and extend to the outside of lug (3), the top fixed mounting of turning block (4) has the pressure piece (5), the bottom fixed mounting of turning block (4) has pressure piece (6), turning block (4) and the internal surface of pressing piece (5) all with the surface swing joint of bottle lid (2), the top of pressure piece (6) and the bottom swing joint of bottle lid (2), joint groove (7) have been seted up at the top of bottle (1), the internal surface of joint groove (7) and the surface swing joint of bottle lid (2), the inner chamber fixed mounting of bottle lid (2) has sealing glue layer (8).

2. The stem cell filtration and separation device according to claim 1, wherein: the inner cavity of the bottle body (1) is movably provided with a separation bottle (9), and both sides of the separation bottle (9) are provided with filter holes (10) in a penetrating way.

3. The stem cell filtration and separation device according to claim 1, wherein: all movable mounting have magnet fixing device (11) around bottle (1), magnet fixing device (11) are including solid fixed ring (12), the bottom fixed mounting of solid fixed ring (12) has permanent magnet (13).

4. The stem cell filtration and separation device according to claim 3, wherein: the equal slidable mounting in both sides at solid fixed ring (12) top has and draws piece (14), two draw the equal fixed mounting in one side that piece (14) is relative to have fixed block (15), fixed slot (16) have all been seted up to the both sides of bottle (1), two the outside that solid fixed ring (12) and fixed ring (12) are extended to all being run through to fixed block (15) one side that is relative, the internal surface sliding connection of the surface of fixed block (15) and fixed slot (16).

5. The stem cell filtration and separation device according to claim 4, wherein: the equal fixed mounting in both sides of solid fixed ring (12) inner chamber has spring (17), two fixed block (15) relative one side all runs through solid fixed ring (12) and extends to the outside of solid fixed ring (12), the surface of fixed block (15) is established, two with the internal surface of spring (17) is overlapped the surface fixed connection of spring (17) relative one side all with two fixed blocks (15).

6. The stem cell filtration and separation device according to claim 4, wherein: the bottom fixed mounting who draws piece (14) has stopper (18), spacing groove (19) have all been seted up to the both sides of solid fixed ring (12) and the below that is located and draws piece (14), the surface of stopper (18) and the internal surface sliding connection of spacing groove (19).