CN214201198U

CN214201198U - Electrochemical luminescence detection device

Info

Publication number: CN214201198U
Application number: CN202022844593.3U
Authority: CN
Inventors: 王梓潼; 杨尚京; 罗建超; 阮德明
Original assignee: Guangdong Dingcheng Electronic Technology Co ltd
Current assignee: Guangdong Dingcheng Electronic Technology Co ltd
Priority date: 2020-12-01
Filing date: 2020-12-01
Publication date: 2021-09-14
Anticipated expiration: 2030-12-01

Abstract

The utility model discloses an electrochemiluminescence detection device, which comprises a detection pool, a photomultiplier and a fixed seat, wherein the bottom of the detection pool is provided with a light-transmitting through hole, the detection pool is provided with a space to be detected for installing an object to be detected, and an annular step is formed between the space to be detected and the side wall of the light-transmitting through hole; the photomultiplier is arranged below the detection cell, and the detection end of the photomultiplier is opposite to the light-transmitting through hole; the fixing seat is connected with the bottom of the detection pool, and an installation space for installing the photomultiplier is formed between the fixing seat and the detection pool. Through putting into the space that awaits measuring with the determinand, can set up analytical equipment in the top of detecting the pond, because photomultiplier is located the determinand below, consequently photomultiplier can not hinder analytical equipment's operation when collecting determinand data through the printing opacity through-hole, improves whole detection precision. In addition, the photomultiplier is fixed in the installation space, so that the photomultiplier is prevented from shifting in the detection process, and the detection accuracy is improved.

Description

Electrochemical luminescence detection device

Technical Field

The utility model relates to an electrochemiluminescence detection device in the technical field of electrochemiluminescence.

Background

Electrochemiluminescence (ECL) is a phenomenon in which luminescence is generated by an electrochemically excited reaction, and is a product in which a CL method and an electrochemical method are combined with each other. The Scanning Electrochemical microscope (SECM) combines the micro-scale spatial position control and the Electrochemical information acquisition, not only can research heterogeneous reaction kinetics on a probe and a substrate and homogeneous reaction kinetics in a solution, distinguish Electrochemical heterogeneity of micro-regions on the surface of an electrode, give the appearance of the surface of a conductor and an insulator, but also can even micro-machine materials and research a plurality of important biological processes and the like. The application of SECM enables the detection of rapid and dynamic processes in many important biological and chemical systems, such as chemical sensors, drug release in pharmacology, phase transfer catalysis, simulated biofilms, etc.

The SECM controls the scanning probe to collect electrochemical information of the base of the detection pool, the scanning electrochemical microscope is used with electrochemical luminescence, the scanning probe is used for exciting chemiluminescence, and then the photomultiplier is used for collecting light signals of electric excitation. There are currently few commercially available instruments that combine the two. In an electrochemiluminescence signal acquisition clock, ITO and FTO are mostly adopted as substrates for large-area imaging such as electrode surface activity, roughness imaging, current density distribution and the like. The method mainly applies fixed excited electric signals to a substrate, and optical signals are acquired by the CCD or EMCCD for one time, although the method can quickly acquire a large-area, the method cannot acquire single cells or fixed modification points, and the CCD or EMCCD is limited by the limit of the optical diffraction limit radius, so that the resolution and the sensitivity are low.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to solve one of the technical problem that exists among the prior art at least, provide an electrochemiluminescence detection device, can cooperate other analytical equipment, effectively reduce measuring error.

According to an embodiment of the present invention, there is provided an electrochemiluminescence detection apparatus, including a detection cell, wherein a light transmission through hole is formed at the bottom of the detection cell, the detection cell is provided with a space to be measured for installing an object to be measured, and an annular step is formed between the space to be measured and a side wall of the light transmission through hole; the photomultiplier is arranged below the detection cell, and the detection end of the photomultiplier is opposite to the light-transmitting through hole; the fixing base, the fixing base with the bottom of detection cell is connected, the fixing base with form the installation space that is used for installing photomultiplier between the detection cell.

According to the utility model discloses an embodiment, furtherly, the detection pond includes cell body and base, the bottom surface of cell body is equipped with first through-hole, the base is equipped with and is used for supporting the accepting platform of cell body, the accepting platform is equipped with the second through-hole, first through-hole with the second through-hole is relative and forms the printing opacity through-hole.

According to the utility model discloses an embodiment, furtherly, the space of awaiting measuring is located first through-hole with between the second through-hole, the space of awaiting measuring with the cell body and/or be equipped with the sealing member between the bearing platform.

According to the utility model discloses an embodiment, furtherly, electrochemiluminescence detection device still includes second working electrode, second working electrode installs detect the pond, second working electrode includes electrode holder, wire and elastic probe, the first end of probe meets with the space that awaits measuring, the second end of probe is fixed in the electrode holder, the first end of wire is fixed the electrode holder and with the second end of probe meets, the second end of wire stretches out detect the pond.

According to the utility model discloses an embodiment, furtherly, second working electrode installs the cell body with between the base, the outside of cell body is equipped with can the holding the cavity of electrode holder, the lateral wall of base is equipped with the wire through-hole, the second end of wire passes through the wire through-hole stretches out detect the pond.

According to the utility model discloses an embodiment, furtherly, the cell body passes through annular fixed cover to be fixed on the base, the cell body is installed in the base, the periphery side of fixed cover is equipped with stair structure, stair structure's path end face with the top looks butt of cell body lateral wall, stair structure's path section with the medial surface threaded connection of base.

According to the utility model discloses an embodiment, furtherly, the fixing base with the connection can be dismantled to the base.

According to the utility model discloses an embodiment, furtherly, the fixing base includes positioning seat and base, the positioning seat is located the base with between the base, be equipped with on the positioning seat and hold the positioning hole that photomultiplier runs through.

According to the utility model discloses an embodiment, furtherly, the base pass through the bolt with the positioning seat is fixed, be equipped with the recess in the base, the lower part of positioning seat stretches into in the recess, the outside of recess is equipped with fixing hole more than two, the bolt runs through behind the fixing hole with the positioning seat butt.

The utility model has the advantages that: through putting into the space that awaits measuring with the determinand, can set up analytical equipment in the top of detecting the pond, because photomultiplier is located the determinand below, consequently photomultiplier can not hinder analytical equipment's operation when collecting determinand data through the printing opacity through-hole, improves whole detection precision. In addition, the photomultiplier is fixed in the installation space, so that the photomultiplier is prevented from shifting in the detection process, and the detection accuracy is improved.

Drawings

In order to more clearly illustrate the technical solution in the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly described below. It is clear that the described figures represent only some embodiments of the invention, not all embodiments, and that a person skilled in the art can also derive other designs and figures from these figures without inventive effort.

Fig. 1 is a schematic view of the overall structure of the present invention;

fig. 2 is a front sectional view of the present invention;

fig. 3 is an exploded view of the present invention.

Detailed Description

This section will describe in detail the embodiments of the present invention, preferred embodiments of the present invention are shown in the attached drawings, which are used to supplement the description of the text part of the specification with figures, so that one can intuitively and vividly understand each technical feature and the whole technical solution of the present invention, but they cannot be understood as the limitation of the protection scope of the present invention.

In the description of the present invention, it should be understood that the orientation or positional relationship indicated with respect to the orientation description, such as up, down, front, rear, left, right, etc., is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

In the description of the present invention, a plurality of means are one or more, a plurality of means are two or more, and the terms greater than, less than, exceeding, etc. are understood as not including the number, and the terms greater than, less than, within, etc. are understood as including the number. If the first and second are described for the purpose of distinguishing technical features, they are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.

In the description of the present invention, unless there is an explicit limitation, the words such as setting, installation, connection, etc. should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above words in combination with the specific contents of the technical solution.

Referring to fig. 1 to 3, the electrochemiluminescence detection apparatus according to the embodiment of the present invention includes a detection cell, a photomultiplier 20, and a fixing base.

The bottom of the detection pool is provided with a light-transmitting through hole, the detection pool is provided with a space to be detected for installing the object to be detected 13, and an annular step is formed between the space to be detected and the side wall of the light-transmitting through hole. In this embodiment, the object to be detected 13 is conductive glass, and after the object to be detected 13 is placed in the space to be detected, the object to be detected 13 can seal the light-transmitting through hole, so as to ensure that the solution in the detection cell does not flow away from the light-transmitting through hole. Optionally, the detection tank includes a tank body 11 and a base 10, a first through hole is formed in a bottom surface of the tank body 11, a receiving table for supporting the tank body 11 is provided on the base 10, a second through hole is formed in the receiving table, and the first through hole and the second through hole are opposite to each other and form a light-transmitting through hole. Cell body 11 and base 10 are independent each other, through changing different cell bodies 11, adaptable different shapes, the determinand 13 of size to satisfy the not co-altitude experiment and detect the demand. The space to be measured is located between the first through hole and the second through hole, and the bottom surface of the tank body 11 and the top surface of the bearing table compress the object to be measured 13 together to prevent the object to be measured 13 from shifting. Further, a sealing element is arranged between the space to be measured and the tank body 11 and/or the receiving platform, the sealing element can be a sealing ring, and the diameter of the sealing ring is larger than that of the light-transmitting through hole. Optionally, the following three specific structures are included: firstly, a sealing element is arranged between the space to be measured and the tank body 11; a sealing element is arranged between the space to be measured and the bearing table; and thirdly, sealing parts are respectively arranged between the space to be measured and the tank body 11 and between the space to be measured and the receiving platform. In this embodiment, the space to be measured respectively with cell body 11 and accept the platform between all be equipped with the sealing member, after the space to be measured is packed into to be measured to determinand 13, the top surface of determinand 13 and the bottom surface of cell body 11 extrude the sealing member jointly, simultaneously, the bottom surface of determinand 13 also extrudes the sealing member jointly with the accept platform to guarantee better that the solution in the cell body 11 can not flow away through the clearance between printing opacity through-hole and the determinand 13, avoid influencing photomultiplier 20's normal operating.

Further, the tank body 11 is fixed on the base 10 through an annular fixing sleeve 12, the tank body 11 is installed in the base 10, a stepped structure is arranged on the outer peripheral side of the fixing sleeve 12, the end face of the small-diameter end of the stepped structure is abutted to the top end of the side wall of the tank body 11, and the small-diameter section of the stepped structure is in threaded connection with the inner side face of the base 10. After the tank body 11 is installed in the base 10, the large-diameter end of the fixing sleeve 12 is held and the small-diameter end of the fixing sleeve 12 is inserted into the base 10, and the fixing sleeve 12 is rotated to connect the external thread of the fixing sleeve 12 with the internal thread of the inner side surface of the base 10, so that the tank body 11 is stably fixed in the base 10. Further, the shoulder of the stepped structure abuts against the top end of the side wall of the base 10, so as to enhance the fastening fit between the fixing sleeve 12 and the base 10.

The photomultiplier 20 is installed below the detection cell, and the detection end of the photomultiplier 20 is opposite to the light-transmitting through hole. Because photomultiplier 20 is in the below of detection cell, consequently can be right the utility model discloses a use is extended, if sets up analytical equipment such as electrochemistry scanning microscope in the top of detection cell and detects, photomultiplier 20 can not influence other analytical equipment's setting and use, has strengthened the suitability. In this embodiment, an electrochemical scanning microscope is disposed above the cell body 11, and the electrochemical scanning microscope is effectively matched with the scanning probe while using the photomultiplier 20 for detection, so as to reduce the collection error of the detection data. After the object 13 is placed in the space to be measured, the detecting end of the photomultiplier 20 can be aligned with the portion of the object 13 in the light-transmitting through hole, thereby collecting data required for detection. The fixing seat is connected with the bottom of the detection pool, and an installation space for installing the photomultiplier 20 is formed between the fixing seat and the detection pool. The photomultiplier 20 is fixed in the installation space and is not easily displaced, so that the accuracy and stability of the collected data can be effectively ensured.

Further, the fixing base is detachably connected to the base 10 to facilitate replacement of the photomultiplier tube 20. The fixing base includes positioning seat 21 and base 22, and positioning seat 21 is located between base 10 and the base 22, is equipped with the locating hole that can hold photomultiplier 20 to run through on the positioning seat 21, and photomultiplier 20 is spacing in locating hole, avoids photomultiplier 20 to take place the displacement and influence the detection. The upper part of the positioning seat 21 is connected with the lower part of the base 10 by screw threads, and the base 22 is fixedly connected with the lower part of the positioning seat 21, so that the photomultiplier 20 is prevented from falling off from the lower part of the positioning seat 21. Optionally, the base 22 is fixed to the positioning seat 21 by bolts, a groove is formed in the base 22, the lower portion of the positioning seat 21 extends into the groove, two or more fixing through holes 23 are formed in the outer side of the groove, and the bolts penetrate through the fixing through holes 23 and then abut against the positioning seat 21. The fixing through holes 23 are arranged at equal intervals along the circumferential direction of the base 22, so that the position of the positioning seat 21 relative to the base 22 is not easily deviated.

In certain embodiments, the electrochemiluminescence detection device further comprises a second working electrode, and bulk imaging is enabled by applying a constant potential to the second working electrode. The second working electrode is arranged in the detection pool and comprises an electrode holder 30, a lead and an elastic probe 31, the probe 31 is a spring conductive probe, the first end of the probe 31 is connected with a space to be detected, the second end of the probe 31 is fixed in the electrode holder 30, the first end of the lead is fixed on the electrode holder 30 and connected with the second end of the probe 31, and the second end of the lead extends out of the detection pool. When the object 13 is placed in the space to be tested, the probe 31 can be connected to the object 13 to be tested, and the electrical signal generated on the object 13 to be tested is conducted to the circuit through the wire. Specifically, the second working electrode is installed between the cell body 11 and the base 10, a concave cavity capable of accommodating the electrode holder 30 is formed in the outer side of the cell body 11, a lead through hole 32 is formed in the side wall of the base 10, and the second end of the lead extends out of the detection cell through the lead through hole 32.

While the preferred embodiments of the present invention have been described in detail, it will be understood by those skilled in the art that the invention is not limited to the details of the embodiments shown, but is capable of various modifications and substitutions without departing from the spirit of the invention.

Claims

1. An electrochemiluminescence detection apparatus, comprising:

the bottom of the detection pool is provided with a light-transmitting through hole, the detection pool is provided with a space to be detected for installing an object to be detected (13), and an annular step is formed between the space to be detected and the side wall of the light-transmitting through hole;

the photomultiplier (20), the photomultiplier (20) is installed below the detection cell, the detection end of the photomultiplier (20) is opposite to the light-transmitting through hole;

the fixing base, the fixing base with the bottom of detection cell is connected, the fixing base with form the installation space that is used for installing photomultiplier (20) between the detection cell.

2. The electrochemiluminescence detection apparatus according to claim 1, wherein: the detection tank comprises a tank body (11) and a base (10), a first through hole is formed in the bottom surface of the tank body (11), the base (10) is provided with a bearing platform used for supporting the tank body (11), a second through hole is formed in the bearing platform, and the first through hole is opposite to the second through hole and forms the light transmission through hole.

3. The electrochemiluminescence detection apparatus according to claim 2, wherein: the space to be measured is located between the first through hole and the second through hole, and a sealing piece is arranged between the space to be measured and the tank body (11) and/or the bearing platform.

4. The electrochemiluminescence detection apparatus according to claim 2, wherein: electrochemiluminescence detection device still includes second working electrode, second working electrode installs the detection cell, second working electrode includes electrode holder (30), wire and elastic probe (31), the first end of probe (31) meets with the space that awaits measuring, the second end of probe (31) is fixed in electrode holder (30), the first end of wire is fixed electrode holder (30) and with the second end of probe (31) meets, the second end of wire stretches out the detection cell.

5. The electrochemiluminescence detection apparatus according to claim 4, wherein: the second working electrode is installed cell body (11) with between base (10), the outside of cell body (11) is equipped with can the holding the cavity of electrode holder (30), the lateral wall of base (10) is equipped with wire through-hole (32), the second end of wire passes through wire through-hole (32) stretch out detect the pond.

6. The electrochemiluminescence detection apparatus according to claim 2, wherein: cell body (11) are fixed through annular fixed cover (12) on base (10), cell body (11) are installed in base (10), the periphery side of fixed cover (12) is equipped with stair structure, stair structure's minor diameter end face with the top looks butt of cell body (11) lateral wall, stair structure's minor diameter section with the medial surface threaded connection of base (10).

7. The electrochemiluminescence detection apparatus according to claim 2, wherein: the fixed seat is detachably connected with the base (10).

8. The electrochemiluminescence detection apparatus according to claim 7, wherein: the fixing seat comprises a positioning seat (21) and a base (22), the positioning seat (21) is located between the base (10) and the base (22), and a positioning through hole capable of allowing the photomultiplier (20) to penetrate through is formed in the positioning seat (21).

9. The electrochemiluminescence detection apparatus according to claim 8, wherein: the base (22) is fixed with the positioning seat (21) through a bolt, a groove is formed in the base (22), the lower portion of the positioning seat (21) extends into the groove, more than two fixing through holes (23) are formed in the outer side of the groove, and the bolt penetrates through the fixing through holes (23) and then abuts against the positioning seat (21).