CN214193285U

CN214193285U - Liquid supplementing device for in-vitro simulated cell culture

Info

Publication number: CN214193285U
Application number: CN202022357252.3U
Authority: CN
Inventors: 曾炳
Original assignee: Nanjing Bingpeng Biotechnology Co ltd
Current assignee: Nanjing Bingpeng Biotechnology Co ltd
Priority date: 2020-10-21
Filing date: 2020-10-21
Publication date: 2021-09-14
Anticipated expiration: 2030-10-21

Abstract

The utility model discloses an in vitro simulation is solution infusion device for cell culture relates to cell culture technical field, for solving the problem that current solution infusion device for cell culture can not add the culture solution regularly and quantitatively. The fixed fluid infusion section of thick bamboo that is provided with in one side of bin upper end, the inside of fluid infusion section of thick bamboo is provided with the carriage release lever, carriage release lever and fluid infusion section of thick bamboo sliding connection, and the upper end of carriage release lever extends to the top of fluid infusion section of thick bamboo, the fixed piston that is provided with of lower extreme of carriage release lever, servo motor is installed to one side of fluid infusion section of thick bamboo upper end, and servo motor is located the inside of fluid infusion section of thick bamboo, be provided with the liquid outlet in the intermediate position department of fluid infusion section of thick bamboo lower extreme, and the liquid outlet extends to the inside of bin, the lower extreme of liquid outlet is sealed to be fixed with the fluid infusion hose, the sealed fluid infusion mouth that is fixed with of one end of fluid infusion hose, timing power is installed to one side of bin.

Description

Liquid supplementing device for in-vitro simulated cell culture

Technical Field

The utility model relates to a cell culture technical field specifically is an in vitro simulation is fluid infusion device for cell culture.

Background

Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function. Cell culture, also called cell cloning technology, is an essential process for the whole biotechnology, and is itself a large-scale cloning of cells. The cell culture technique can become simple unicellular or the few cell of differentiation through a large amount of cultivations by a cell, at the cultivation in-process of cell, can consume the inside culture solution of culture apparatus, consequently need regularly to culture apparatus's inside addition culture solution, leads to external world and inside to contact for avoiding opening culture apparatus simultaneously, so need use the moisturizing device for cell culture, guarantees that culture apparatus does not contact with the external world under the prerequisite, realizes adding the culture solution.

However, most of the conventional fluid infusion devices for cell culture require manual operations of timing and quantification, and on one hand, the interval time for adding the culture fluid is greatly influenced by personal factors, and on the other hand, the amount of the culture fluid is not easy to be controlled, so that the conventional requirements are not met.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide an in vitro simulation is fluid infusion device for cell culture to the problem that the fluid infusion device for cell culture who proposes in the solution above-mentioned background art can not add the culture solution regularly and quantitatively.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides an external simulation is fluid infusion device for cell culture, includes the bin, the fixed fluid infusion section of thick bamboo that is provided with in one side of bin upper end, the inside of fluid infusion section of thick bamboo is provided with the carriage release lever, carriage release lever and fluid infusion section of thick bamboo sliding connection, and the upper end of carriage release lever extends to the top of fluid infusion section of thick bamboo, the fixed piston that is provided with of lower extreme of carriage release lever, servo motor is installed to one side of fluid infusion section of thick bamboo upper end, and servo motor is located the inside of fluid infusion section of thick bamboo, be provided with the liquid outlet in the intermediate position department of fluid infusion section of thick bamboo lower extreme, and the liquid outlet extends to the inside of bin, the lower extreme of liquid outlet is sealed to be fixed with the fluid infusion hose, the sealed fluid infusion mouth that is fixed with of one end of fluid infusion hose, timing power source is installed to one side of bin, and timing power source passes through the screw fixation with the bin.

Preferably, one side of the movable rod is provided with a tooth groove and a sliding groove respectively, a gear is installed between the tooth groove and the sliding groove and meshed with the tooth groove, and an output shaft of the servo motor is in transmission connection with one end of the gear through a coupler.

Preferably, the fixed flow sensor that is provided with in intermediate position department of liquid outlet, one side fixed mounting inside the bin has the singlechip, and the output of singlechip and the input electric connection of timing power, flow sensor's output and singlechip input electric connection.

Preferably, one end of the fluid infusion hose close to the fluid infusion nozzle is provided with a one-way valve.

Preferably, the front end of the liquid supplementing cylinder is provided with scale marks, and one side of the liquid supplementing cylinder is fixedly provided with a liquid adding pipe in a sealing manner.

Preferably, a box door is installed at the front end of the storage box and is rotatably connected with the storage box through a hinge, and a window is fixedly arranged inside the box door.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the utility model has the advantages that the servo motor is arranged on one side of the upper end of the liquid supplementing cylinder, the output shaft of the servo motor drives the shaft coupling to rotate by starting the servo motor, and then the gear in the movable rod is driven to rotate by the transmission of the coupler, one side of the movable rod is respectively provided with a tooth socket and a sliding chute, the sliding chute limits the gear on one hand and cooperates with the rotation of the gear on the other hand, under the positive and negative driving of the servo motor, the meshing action of the gear and the tooth socket converts the rotary motion into linear motion, the gear drives the movable rod to move up and down, when the liquid is replenished, the servo motor rotates positively, the movable rod drives the piston to move downwards gradually, thereby extrude the inside culture solution of fluid infusion section of thick bamboo, through this kind of mode, can reduce the manual burden of carrying out the fluid infusion of staff, servo motor transmission is stable simultaneously, and the fluid infusion process is comparatively smooth.

2. The utility model discloses an install timing power supply in one side of bin, the staff sets for timing power supply according to the fluid infusion interval to fluid infusion device, when timing power supply reachs the appointed time, just can start servo motor, make servo motor forward rotation, the carriage release lever that the piston was equipped with is made a command to extrude the culture solution, through installing flow sensor at the liquid outlet, flow sensor can detect through the culture solution volume, when reaching the settlement numerical value, with signal transmission to singlechip, make the singlechip close timing power supply, thereby make servo motor stop work, accomplish the ration to the inside culture solution of culture apparatus and add, through this kind of mode, the interval time of adding the culture solution on the one hand obtains comparatively accurate fixed, on the other hand can carry out the accuse to the volume of adding the culture solution, provide good guarantee for cell culture.

Drawings

Fig. 1 is an overall front view of the present invention;

FIG. 2 is a schematic view of the overall internal structure of the present invention;

FIG. 3 is a partial enlarged view of area A of the present invention;

fig. 4 is a schematic view of a local structure of the moving rod of the present invention.

In the figure: 1. a storage tank; 2. a box door; 3. a window; 4. a fluid infusion cylinder; 5. a liquid feeding pipe; 6. scale lines; 7. a travel bar; 8. a timing power supply; 9. a piston; 10. a single chip microcomputer; 11. a liquid outlet; 12. a flow sensor; 13. a fluid infusion hose; 14. a one-way valve; 15. a liquid replenishing nozzle; 16. a servo motor; 17. a coupling; 18. a gear; 19. a tooth socket; 20. a chute.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

Referring to fig. 1-4, the present invention provides an embodiment: the utility model provides an external simulation is fluid infusion device for cell culture, including bin 1, the fixed fluid infusion section of thick bamboo 4 that is provided with in one side of bin 1 upper end, the inside of fluid infusion section of thick bamboo 4 is provided with carriage release lever 7,

carriage release lever

7 and 4 sliding connection of fluid infusion section of thick bamboo, and the upper end of carriage release lever 7 extends to the top of fluid infusion section of thick bamboo 4, the fixed piston 9 that is provided with of lower extreme of carriage release lever 7, servo motor 16 is installed to one side of fluid infusion section of thick bamboo 4 upper end, and servo motor 16 is located the inside of fluid infusion section of thick bamboo 4, intermediate position department in the lower extreme of fluid infusion section of thick bamboo 4 is provided with liquid outlet 11, and liquid outlet 11 extends to the inside of bin 1, the sealed fluid infusion hose 13 that is fixed with of the lower extreme of liquid outlet 11, the sealed fluid infusion mouth 15 that is fixed with in one end of fluid infusion hose 13, timing power supply 8 is installed to one side of bin 1, and timing power supply 8 passes through the fixed screw with bin 1.

Furthermore, one side of the moving rod 7 is provided with a tooth groove 19 and a sliding groove 20 respectively, a gear 18 is installed between the tooth groove 19 and the sliding groove 20, the gear 18 is meshed with the tooth groove 19, an output shaft of the servo motor 16 is in transmission connection with one end of the gear 18 through a coupler 17, and by means of the mode, the burden of manual liquid supplementing of a worker can be reduced, meanwhile, the servo motor 16 is stable in transmission, and the liquid supplementing process is smooth.

Further, the fixed flow sensor 12 that is provided with in intermediate position department of liquid outlet 11, one side fixed mounting of bin 1 inside has singlechip 10, and singlechip 10's output and timing power supply 8's input electric connection, flow sensor 12's output and singlechip 10 input electric connection, through this kind of mode, the interval time that adds the culture solution on the one hand obtains comparatively accurate fixed, and on the other hand can carry out the control to the volume of adding the culture solution, provides good guarantee for cell culture.

Further, one end of the fluid infusion hose 13 close to the fluid infusion nozzle 15 is provided with a one-way valve 14, and the one-way valve 14 can prevent the culture fluid from flowing back.

Furthermore, the front end of fluid infusion section of thick bamboo 4 is provided with scale mark 6, and one side of fluid infusion section of thick bamboo 4 is sealed to be fixed with filling tube 5, can know the inside cultivation storage condition of fluid infusion section of thick bamboo 4 through observing scale mark 6, opens the screw cap on the filling tube 5 and can carry out the liquid feeding to fluid infusion section of thick bamboo 4.

Further, chamber door 2 is installed to the front end of bin 1, and chamber door 2 passes through the hinge rotation with bin 1 and is connected, and the inside fixed window 3 that is provided with of chamber door 2.

The working principle is as follows: when the device is used, a worker sets the timing power supply 8 according to the fluid infusion interval of the fluid infusion device, when the timing power supply 8 reaches a specified time, the servo motor 16 is started, the servo motor 16 rotates in the forward direction, the output shaft of the servo motor 16 drives the coupler 17 to rotate, and then the gear 18 inside the moving rod 7 is driven to rotate by the coupler 17, the meshing action of the gear 18 in the moving rod 7 and the tooth groove 19 converts the rotary motion into linear motion, the moving rod 7 drives the piston 9 to move downwards gradually, so that the culture fluid inside the fluid infusion cylinder 4 is extruded out, the flow sensor 12 is arranged at the fluid outlet 11, the flow sensor 12 can detect the amount of the culture fluid passing through, when the set value is reached, a signal is transmitted to the single chip microcomputer 10, the single chip microcomputer 10 closes the timing power supply 8, and the servo motor 16 stops working, accomplish the ration of adding culture solution to the culture apparatus inside and add, the interval time of adding the culture solution obtains comparatively accurate fixed on the one hand, and on the other hand can handle the accuse to the volume of adding the culture solution, provides good guarantee for cell culture.

It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. The utility model provides an in vitro simulation is fluid infusion device for cell culture, includes bin (1), its characterized in that: a liquid supplementing cylinder (4) is fixedly arranged on one side of the upper end of the storage box (1), a moving rod (7) is arranged inside the liquid supplementing cylinder (4), the moving rod (7) is connected with the liquid supplementing cylinder (4) in a sliding manner, the upper end of the moving rod (7) extends to the upper side of the liquid supplementing cylinder (4), a piston (9) is fixedly arranged at the lower end of the moving rod (7), a servo motor (16) is installed on one side of the upper end of the liquid supplementing cylinder (4), the servo motor (16) is located inside the liquid supplementing cylinder (4), a liquid outlet (11) is arranged at the middle position of the lower end of the liquid supplementing cylinder (4), the liquid outlet (11) extends to the inside of the storage box (1), a liquid supplementing hose (13) is fixed at the lower end of the liquid outlet (11) in a sealing manner, a liquid supplementing nozzle (15) is fixed at one end of the liquid supplementing hose (13) in a sealing manner, a timing power supply (8) is installed on one side of the storage box (1), and the timing power supply (8) and the storage box (1) are fixed through screws.

2. The fluid infusion device for in vitro simulated cell culture according to claim 1, wherein: one side of carriage release lever (7) is provided with tooth's socket (19) and spout (20) respectively, install gear (18) between tooth's socket (19) and spout (20), and gear (18) and tooth's socket (19) meshing are connected, shaft coupling (17) transmission connection is passed through with the one end of gear (18) to the output shaft of servo motor (16).

3. The fluid infusion device for in vitro simulated cell culture according to claim 1, wherein: the utility model discloses a storage case, including bin (1), bin (11), the intermediate position department of liquid outlet (11) is fixed and is provided with flow sensor (12), one side fixed mounting of bin (1) inside has singlechip (10), and the output of singlechip (10) and the input electric connection of timing power supply (8), the output and the singlechip (10) input electric connection of flow sensor (12).

4. The fluid infusion device for in vitro simulated cell culture according to claim 1, wherein: one end of the fluid infusion hose (13) close to the fluid infusion nozzle (15) is provided with a one-way valve (14).

5. The fluid infusion device for in vitro simulated cell culture according to claim 1, wherein: the front end of the liquid supplementing cylinder (4) is provided with scale marks (6), and one side of the liquid supplementing cylinder (4) is hermetically fixed with a liquid adding pipe (5).

6. The fluid infusion device for in vitro simulated cell culture according to claim 1, wherein: the refrigerator is characterized in that a refrigerator door (2) is installed at the front end of the storage box (1), the refrigerator door (2) is rotatably connected with the storage box (1) through a hinge, and a window (3) is fixedly arranged inside the refrigerator door (2).