CN214150109U

CN214150109U - Separator is gathered to sample ration

Info

Publication number: CN214150109U
Application number: CN202022859180.2U
Authority: CN
Inventors: 王滨; 辛杰; 王慧
Original assignee: Baonuowei Suzhou Biotechnology Co ltd
Priority date: 2020-12-03
Filing date: 2020-12-03
Publication date: 2021-09-07
Anticipated expiration: 2030-12-03

Abstract

The utility model discloses a separator is gathered to sample ration, include, the sampling pipe body, set up respectively in the sampling stick and the filter tube at sampling pipe body both ends, this internal piston of sampling pipe is provided with one and helps the push rod, help having seted up quantitative hole on the push rod, the sampling stick wears to locate help in the push rod, the sampling stick is in help the push rod internal motion to make the distal end of sampling stick alternates in quantitative hole both sides. The utility model discloses outstanding effect does: the utility model discloses a collection separator need not centrifuge, power, separation, processing sample, fine realization on-the-spot quantitative sampling that at home, on-the-spot just can be quick to can carry out long-range transportation, can directly dock on instruments such as full-automatic biochemical appearance, full-automatic specific protein analysis appearance, need not special transformation instrument and just can realize automated inspection.

Description

Separator is gathered to sample ration

Technical Field

The utility model relates to a separator is gathered to sample ration.

Background

In medical examination, detection is divided into quantitative detection and qualitative detection. Quantitative assays are typically used to determine the amount of a particular pathogen or antibody. The primary conditions for quantitative detection are quantitative sampling and pretreatment of quantitative samples, including dissolution and separation. Aiming at a sample to be detected containing a large amount of insoluble substances, soluble substances to be detected need to be separated out firstly, such as: microorganisms, proteins, organic substances, inorganic substances, nucleic acids, etc. in foods; blood, proteins, nucleic acids, etc. in feces. In order to ensure the consistency of each sample to be detected, the sampling process must be quantitative, but the field weighing and sampling of each sample such as excrement, food residues and the like is unrealistic and is not easy to operate.

In the processes of food safety detection, fecal samples are widely used in medical, zoology, criminal investigation, biology and chemistry detection as samples to be detected, the samples to be detected in food and feces are usually dissolved out and centrifuged to remove sediments, and some samples need to be added with preservatives, stabilizers, purification, adsorption, buffer solutions and the like. Moreover, clinical application proves that quantitative detection of fecal occult blood is more clinically significant for clinical diagnosis. For example, Fecal Occult Blood (FOB) is the first choice for screening and testing intestinal cancer. Regardless of the detection methodology, the dissolution and extraction of occult blood in the sample and the filtration of fecal sediments are all essential processes. The utility model is simple, practical, does not need a centrifugal device and can be directly butted with the existing instruments such as a full-automatic biochemical analyzer and a full-automatic specific protein analyzer, the automatic detection can be realized without special modification of the instruments, and the practical value is very high.

SUMMERY OF THE UTILITY MODEL

In view of the above-mentioned defects in the prior art, the present invention is directed to a separator for quantitatively collecting samples.

The purpose of the utility model is to realize through the following technical scheme:

the utility model provides a separator is gathered to sample ration, includes, the sampling pipe body, sets up respectively in sampling stick and the filter tube at sampling pipe body both ends, this internal piston of sampling pipe is provided with one and helps the push rod, help and seted up quantitative hole on the push rod, the sampling stick wears to locate in the help push rod, the sampling stick is in help the push rod internal motion to make the distal end of sampling stick is changeed in quantitative hole both sides.

Preferably, the far end of the push-aid rod is arranged in the sampling tube body, the far end of the push-aid rod is provided with a flexible pad, the far end of the push-aid rod is provided with a quantitative hole, and the far end of the sampling rod is in clearance fit with the quantitative hole in the push-aid rod and extends towards the direction of the filter tube.

Preferably, the filter tube comprises a tube body arranged in the sampling tube body, a filter membrane is arranged at one end of the tube body, and a sealing membrane is arranged at the other end of the tube body.

Preferably, the filter membrane end of the tube body of the filter tube is equivalent to the inner diameter of the sampling tube body, and the diameter of the sealing membrane end of the tube body is larger than that of the sampling tube body.

Preferably, the distal end surface of the sampling rod is concave-convex, and the total concave volume is equivalent to the required sampling amount.

Preferably, a dissolving liquid is arranged between the distal end of the push-assisting rod and the filter pipe.

Preferably, one end face of the filter membrane is pasted with a bonding glue layer.

Preferably, the aperture of the quantification aperture is comparable to the outer diameter of the distal end of the sampling wand.

Preferably, a through hole is formed in the push-aid rod, the through hole is communicated with the quantifying hole, the diameter of the through hole is larger than that of the quantifying hole, and the sampling rod is arranged in the through hole in a penetrating mode.

The utility model discloses outstanding effect does: the utility model discloses a collection separator need not centrifuge, power, and easy operation, separation, processing sample that at home, scene just can be quick, fine realization on-the-spot quantitative sample to can carry out long-range transportation, can directly dock on instruments such as full-automatic biochemical appearance, full-automatic specific protein analysis appearance that need not special transformation instrument and just can realize automated inspection.

The following detailed description is made of specific embodiments of the present invention with reference to the accompanying drawings, so as to make the technical solution of the present invention easier to understand and master.

Drawings

Fig. 1 is a schematic structural diagram of an embodiment of the present invention.

Wherein, 1 sampling pipe body, 2 sampling stick, 3 filter tubes, 5 helping push rods, 11 helping push rod distal ends, 21 sampling stick distal ends, 51 wear to establish the hole, 52 ration hole.

Detailed Description

The utility model discloses a separator is gathered to sample ration combines fig. 1, include, sampling pipe body 1, set up respectively in the sampling stick and the filter tube 3 at 1 both ends of sampling pipe body. An assistant push rod 5 is arranged in the sampling tube body 1 in a piston mode. A sampling rod 2 penetrates through the push-aid rod 5. The length of the sampling rod 2 is greater than that of the push-aid rod 5, the diameter of the near end of the sampling rod 2 is greater than the outer diameter of the push-aid rod 5, and the sampling rod 2 reciprocates in the push-aid rod 5 towards the direction of the filter pipe. In order to dissolve the collected sample, a dissolving liquid is arranged between the distal end 11 of the push-aid rod and the filter tube 3.

Specifically, a through hole 51 is formed in the push-aid rod 5, a quantitative hole 52 is formed in the distal end 11 of the push-aid rod, the through hole 51 is communicated with and coaxial with the quantitative hole 52, the diameter of the through hole 51 is larger than that of the quantitative hole 52, and the sampling rod 2 penetrates into the through hole 51 from one end of the push-aid rod 5 and can penetrate through the quantitative hole 52. The far end of the push-aid rod 5 is arranged in the sampling tube body 1, and the far end of the push-aid rod 5 is provided with a flexible pad.

The diameter of the distal end 21 of the sampling rod is equivalent to the inner diameter of the quantitative hole 52, and the two parts are in clearance fit. The surface of the far end 21 of the sampling rod is concave-convex, and the total concave volume is equivalent to the required sampling amount. After the sampling rod 2 is sampled and passes through the quantitative hole 52, the quantitative hole 52 can scrape the far end of the sampling rod 2 for redundant sampling, and the residual sample is left in the concave part, so that the quantitative effect is achieved.

The filter tube 3 is including arranging the body in the sampling pipe body 1 in, the one end of body is provided with the filter membrane, for realizing filtering, the filter membrane aperture is less than sample sediment diameter, and the material can be non-woven fabrics, various fibre etc. do not do the restriction here. The other end of the tube body is provided with a sealing film, and in order to facilitate subsequent operation, in the embodiment, the sealing film is preferably in an aluminum foil form. In order to achieve a better sealing effect before filtration, one end face of the filter membrane is pasted with a bonding adhesive layer, and the bonding adhesive layer is arranged on the inner side of the filter pipe. The sampling tube body is provided with a filter membrane end, a sealing membrane end and a sampling tube body, wherein the filter membrane end of the tube body is arranged in the sampling tube body, and the sealing membrane end is arranged outside the sampling tube body. The filter membrane end with sampling pipe body internal diameter is equivalent, the membrane end diameter of sealing of body is greater than sampling pipe body diameter. In this embodiment, the filter tube 3 may be disposed in the sampling tube body 1 in a fixed manner. Of course, it can be placed in the sampling tube body 1 in a detachable manner.

The following brief description of the invention discloses a collection separation principle:

firstly, extract sampling stick 2, treat that the sample that detects dips in and get the sample, insert sampling stick distal end 21 in helping push rod 5 to make sampling stick distal end 21 wear to establish quantitative hole 52, quantitative hole 52 will carry out scraping of unnecessary sample to sampling stick distal end 21. After the distal end 21 of the sampling rod enters the dissolution liquid, the test tube can be shaken for better and faster dissolution. After the sample on the sampling rod is dissolved, the sampling rod 2 is pushed again, the push-aid rod 5 is driven to move towards the direction of the filter pipe, certain pressure is formed on the dissolved liquid, the dissolved liquid after the sample is dissolved by the aid of the push-aid rod is pushed into the filter pipe, and finally the dissolved liquid enters the pipe body of the filter pipe after being filtered, so that the collection and separation of the whole sample are completed. And (4) storing and conveying the finished sampling tube body. The dissolving solution is a buffer solution, and the components of the dissolving solution are phosphate buffer solution with the pH value of 7.2, stabilizing agent and preservative. The concentration of the phosphate buffer solution is 0.1-0.5mol/L, the stabilizing agent is 1% -4% of trehalose, 0.05-0.2% of Brj-35, and the preservative is 0.1-0.3% of wt ProClin 300.

The whole collection and separation process is convenient and rapid, the separated sample can be directly used for the detection of an automatic instrument, the detection result cannot be influenced, and the problems of needle blockage and the like of the automatic instrument caused by food and excrement sediments in the prior art are also avoided.

For better embodiment the utility model discloses storage stability after the separator sampling, the following test of stability of having carried out specifically sees table 1.

Use the commercially available sampling pipe respectively with the utility model discloses the same sample is gathered to the collection separator, arranges 2-8 ℃ in and preserves, uses FOB detect reagent box survey, and repeated survey 3 times every day calculates the average value, detects average value relative deviation and surpasses 10% until gained average value and the first day.

Table 1: commercially available sampling pipe and the utility model discloses a collection separator sample storage stability

. Remarking: reported in ng hHb/mL

The relative deviation of a commercially available sampling tube on the 3 rd day is over 10%, and the utility model discloses relative deviation is still in 10% within range on the 7 th day, shows that the utility model discloses sampling tube can show improvement sample storage stability.

The utility model has a plurality of implementation modes, and all technical schemes formed by adopting equivalent transformation or equivalent transformation all fall within the protection scope of the utility model.

Claims

1. A separator is gathered to sample ration which characterized in that: including, the sampling pipe body, set up respectively in sampling stick and the filter tube at sampling pipe body both ends, this internal piston of sampling pipe is provided with one and helps the push rod, help and seted up quantitative hole on the push rod, the sampling stick wears to locate help in the push rod, the sampling stick is in help the push rod internal motion to make the distal end of sampling stick is in quantitative hole both sides transform.

2. A quantitative sample collection separator as claimed in claim 1, wherein: the far end of the push-aid rod is arranged in the sampling tube body, the far end of the push-aid rod is provided with a flexible pad, the far end of the push-aid rod is provided with a quantitative hole, and the far end of the sampling rod is in clearance fit with the quantitative hole of the push-aid rod and extends towards the direction of the filter tube.

3. The separator for quantitative collection of samples according to claim 1, wherein: the filter tube comprises a tube body arranged in the sampling tube body, a filter membrane is arranged at one end of the tube body, and a sealing membrane is arranged at the other end of the tube body.

4. The separator for quantitative collection of samples according to claim 3, wherein: the filter membrane end of the pipe body of the filter pipe is equivalent to the inner diameter of the sampling pipe body, and the diameter of the sealing membrane end of the pipe body is larger than the diameter of the sampling pipe body.

5. The separator for quantitative collection of samples according to claim 1, wherein: the far-end surface of the sampling rod is concave-convex, and the total concave volume is equivalent to the required sampling amount.

6. The separator for quantitative collection of samples according to claim 5, wherein: and a dissolving liquid is arranged between the far end of the boosting rod and the filter pipe.

7. The separator for quantitative collection of samples according to claim 3, wherein: and a bonding glue layer is adhered to one end face of the filter membrane.

8. The separator for quantitative collection of samples according to claim 1, wherein: the aperture of the quantitative hole is equivalent to the outer diameter of the distal end of the sampling rod.

9. The quantitative sample collection separator of claim 8, wherein: the push-aid rod is provided with a through hole, the through hole is communicated with the quantitative hole, the diameter of the through hole is larger than that of the quantitative hole, and the sampling rod is arranged in the through hole in a penetrating mode.