CN214142388U

CN214142388U - Convenient nucleic acid extraction element

Info

Publication number: CN214142388U
Application number: CN202022629209.8U
Authority: CN
Inventors: 孙凤娟; 肖理慧; 王庆专
Original assignee: Shanghai Lingjun Biotechnology Co ltd
Current assignee: Shanghai Lingjun Biotechnology Co ltd
Priority date: 2020-11-13
Filing date: 2020-11-13
Publication date: 2021-09-07
Anticipated expiration: 2030-11-13

Abstract

The utility model discloses a convenient nucleic acid extraction element, concretely relates to nucleic acid extraction technical field, including box body and quintuplet pipe, the inside adsorption apparatus that is equipped with of box body constructs, adsorption apparatus constructs including the outer tube, the inside dead lever that is equipped with of outer tube, the fixed bar magnet that is equipped with of dead lever one end, dead lever one end extends to the outer tube top, it has the aluminum pipe to inlay on the outer tube, the box body passes through threaded connection with the top cap, the outer tube other end passes through the top cap and extends to the top cap top. The utility model discloses a set up adsorption apparatus and construct, extend to quintuplet intraduct with the outer tube, laminate outer tube one end in the outer end of magnetic bead, then fill in dead lever one end in the outer tube together with the bar magnet, the bar magnet adsorbs the magnetic bead, shifts it, when the bar magnet upwards mentions and gets into the aluminum pipe, the aluminum pipe plays the effect of blocking the magnetic force, avoids the bar magnet to remove and drives the magnetic bead roll and fall and cause harmful effects for nucleic acid extraction.

Description

Convenient nucleic acid extraction element

Technical Field

The embodiment of the utility model provides a relate to nucleic acid extraction technical field, concretely relates to convenient nucleic acid extraction element.

Background

With the rapid development of nucleic acid extraction technology, automated nucleic acid extraction devices have also been rapidly developed. At present, a plurality of brands and types of automatic nucleic acid extracting devices are produced in China, the automatic nucleic acid extracting devices are suitable for users with large detection sample amount, and for customers with small detection sample amount, a simple and convenient extracting kit suitable for users with small sample amount does not exist at present.

The magnetic bead method nucleic acid extraction kit is a high and new technology product integrating biological science and nano material science, is a major breakthrough of nucleic acid extraction and purification technology in China, and thoroughly solves the situation that nucleic acid extraction and purification in China depend on import for a long time.

SUMMERY OF THE UTILITY MODEL

For this, the embodiment of the utility model provides a convenient nucleic acid extraction element, through setting up adsorption apparatus structure, extend to the quintuplet intraduct with the outer tube, laminate outer tube one end in the outer end of magnetic bead, then fill in dead lever one end in the outer tube together with the bar magnet, the bar magnet adsorbs the magnetic bead, shift it, when the bar magnet upwards mentions and gets into the aluminum pipe, the effect of magnetic force is played in the separation to the aluminum pipe, avoid the bar magnet to remove and drive the magnetic bead roll and cause harmful effects for nucleic acid extraction, when taking out the bar magnet among the solution prior art probably take place the magnetic bead along with the bar magnet take out and the phenomenon that rolls down produces the problem of influence to the extraction of nucleic acid.

In order to achieve the above object, the embodiment of the present invention provides the following technical solutions: a convenient nucleic acid extraction device comprises a box body and a quintuplet tube, wherein the quintuplet tube is arranged inside the box body, and an adsorption mechanism is arranged inside the box body;

the adsorption mechanism comprises an outer pipe, the outer pipe is arranged inside the box body, a fixing rod is arranged inside the outer pipe, a magnetic rod is fixedly arranged at one end of the fixing rod, one end of the fixing rod extends to the top of the outer pipe, an aluminum pipe is inlaid on the outer pipe, a top cover is arranged at the top end of the box body, the box body is connected with the top cover through threads, and the other end of the outer pipe extends to the top of the top cover through the top cover.

Further, a rotary table is arranged inside the box body, and the bottom end of the rotary table is movably connected with the bottom end inside the box body through a bearing.

Furthermore, a plurality of placing holes and a water tank are formed in the top end of the rotary table, the water tank is arranged on the inner side of the placing holes, and the quintuplet pipe is arranged inside the placing holes.

Furthermore, a connecting pipe is fixedly arranged at the bottom end of the placing hole, and one end of the connecting pipe is connected with the water tank.

Furthermore, the outer end of the rotary table is embedded with a shifting ring, one side of the box body is provided with a groove, and the groove is arranged on one side of the shifting ring.

Furthermore, a finger ring is fixedly arranged at one end of the fixing rod.

Further, the top cap bottom mounting is equipped with the rubber circle, the rubber circle inboard contacts with the box body outer end, the top cap outer end is inlayed and is had the sealing washer, the sealing washer inboard contacts with the outer tube outer end.

The embodiment of the utility model provides a have following advantage:

the utility model discloses a set up adsorption apparatus and construct, extend to quintuplet intraduct with the outer tube, laminate outer tube one end in the outer end of magnetic bead, then fill in dead lever one end in the outer tube together with the bar magnet, the bar magnet adsorbs the magnetic bead, shifts it, when the bar magnet upwards mentions and gets into the aluminum pipe, the aluminum pipe plays the effect of blocking magnetic force, compares with prior art, avoids the electro-magnet to remove and drives the magnetic bead roll and fall and cause harmful effects for nucleic acid extraction.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.

The structure, ratio, size and the like shown in the present specification are only used for matching with the content disclosed in the specification, so as to be known and read by people familiar with the technology, and are not used for limiting the limit conditions which can be implemented by the present invention, so that the present invention has no technical essential significance, and any structure modification, ratio relationship change or size adjustment should still fall within the scope which can be covered by the technical content disclosed by the present invention without affecting the efficacy and the achievable purpose of the present invention.

Fig. 1 is a front view provided by the present invention;

fig. 2 is a cross-sectional view provided by the present invention;

FIG. 3 is a top view of the case provided by the present invention;

FIG. 4 is an enlarged view of the portion A in FIG. 2 according to the present invention;

FIG. 5 is an enlarged view of the part B in FIG. 2 according to the present invention

Fig. 6 is a top view of the top cover provided by the present invention;

in the figure: the device comprises a box body 1, a turntable 2, a placing hole 3, a five-way pipe 4, a groove 5, a shifting ring 6, a connecting pipe 7, a water tank 8, an outer pipe 9, a top cover 10, a sealing ring 11, a fixing rod 12, a magnetic rod 13 and an aluminum pipe 14.

Detailed Description

The present invention is described in terms of specific embodiments, and other advantages and benefits of the present invention will become apparent to those skilled in the art from the following disclosure. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to the attached figures 1-6 of the specification, the convenient nucleic acid extraction device of the embodiment comprises a box body 1 and a quintuplet tube 4, wherein the quintuplet tube 4 is arranged inside the box body 1, and an adsorption mechanism is arranged inside the box body 1;

the adsorption device comprises an outer tube 9, the outer tube 9 is arranged inside a box body 1, a fixing rod 12 is arranged inside the outer tube 9, a magnetic rod 13 is fixedly arranged at one end of the fixing rod 12, one end of the fixing rod 12 extends to the top of the outer tube 9, an aluminum tube 14 is inlaid on the outer tube 9, a top cover 10 is arranged on the top end of the box body 1, the box body 1 is connected with the top cover 10 through threads, and the other end of the outer tube 9 extends to the top of the top cover 10 through the top cover 10.

Further, the inside carousel 2 that is equipped with of

box body

1, 2 bottoms of carousel and the inside bottom of box body 1 pass through bearing swing joint, change the quintuplet pipe 4 that is equipped with different reagents through the rotation of carousel 2.

Furthermore, a plurality of placing holes 3 and a water tank 8 are formed at the top end of the rotating disc 2, the water tank 8 is arranged inside the placing holes 3, the five-way pipes 4 are arranged inside the placing holes 3, and the placing holes 3 are used for placing the five-way pipes 4.

Further, place 3 bottom fixings in hole and be equipped with connecting pipe 7, connecting pipe 7 one end is connected with basin 8, is convenient for carry out water bath heating to quintuplet pipe 4.

Further, 2 outer ends of carousel are inlayed and are had the ring 6 of dialling, recess 5 has been seted up to box body 1 one side, recess 5 is established and is dialled ring 6 one side, dials ring 6 through recess 5 to this drives carousel 2 and rotates.

Further, a finger ring is fixedly arranged at one end of the fixing rod 12, and the finger ring is convenient to fix by using fingers and can be operated by a single hand.

Further, the top cap 10 bottom mounting is equipped with the rubber circle, the rubber circle inboard contacts with 1 outer end of box body, inlay there is sealing washer 11 top cap 10 outer end, 11 inboards of sealing washer contact with 9 outer ends of outer tube, and the rubber circle has improved top cap 10's leakproofness.

The implementation scenario is specifically as follows: opening a top cover 10, placing a quintuplet tube 4 in a placing hole 3 at the top end of a rotary table 2, fixing the quintuplet tube 4 in the rotary table 2, then respectively pouring an extracted sample and corresponding lysis solution, washing solution, eluent and the like into the quintuplet tube 4, then adding magnetic beads into the sample according to steps, simultaneously injecting hot water into a water tank 8, enabling the hot water to enter the placing hole 3 through a connecting pipe 7 at the bottom end of the water tank 8, carrying out water bath heating on the quintuplet tube 4 and the sample therein, next, closing the top cover 10, waiting for a period of time, adsorbing nucleic acid in the sample on the surface by the magnetic beads in the lysis solution, taking out the magnetic beads from the quintuplet tube 4 filled with the lysis solution, moving the magnetic beads into the quintuplet tube 4 filled with the washing solution, pushing an outer tube 9, inserting one end of the outer tube 9 into the quintuplet tube 4 filled with the magnetic beads, then inserting one end of a fixing rod 12 and a magnetic rod 13 into the outer tube 9, when one end of the fixed rod 12 reaches the bottom end of the outer tube 9, when one end of the outer tube 9 contacts the magnetic beads, the magnetic force of the magnetic rod 13 adsorbs the magnetic beads, the outer tube 9 is pulled upwards to drive the magnetic beads to be drawn out from one of the quintuplet tubes 4, then the dial ring 6 is shifted through the groove 5 to drive the rotary table 2 and the quintuplet tubes 4 to rotate, when the quintuplet tubes 4 filled with washing liquid rotate to the bottoms of the magnetic beads, one end of the outer tube 9 and the magnetic beads are plugged into the corresponding quintuplet tubes 4 again, then fingers are inserted into the finger rings to lift the fixed rod 12 upwards to drive the magnetic rod 13 to move upwards, when the magnetic rod 13 moves into the aluminum tube 14, under the partition action of the aluminum tube 14, the magnetic force of the magnetic rod 13 is partitioned to be difficult to adsorb the magnetic beads, the magnetic beads fall down to complete the transfer of the magnetic beads, the outer tube 9 is extended into the quintuplet tubes 4 by arranging an adsorption mechanism, one end of the outer tube 9 is attached to the outer ends of the magnetic beads, then, one end of the fixing rod 12 and the magnetic rod 13 are plugged into the outer tube 9, the magnetic rod 13 adsorbs magnetic beads and transfers the magnetic beads, when the magnetic rod 13 is lifted upwards and enters the aluminum tube 14, the aluminum tube 14 plays a role in blocking magnetic force, adverse effects on nucleic acid extraction caused by the fact that the magnetic rod 13 moves to drive the magnetic beads to roll off are avoided, and the problem that the phenomenon that the magnetic beads roll off along with the magnetic rod when the magnetic rod is pulled out in the prior art can affect the extraction of the nucleic acid is specifically solved.

Although the invention has been described in detail with respect to the general description and the specific embodiments, it will be apparent to those skilled in the art that modifications and improvements can be made based on the invention. Therefore, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims

1. The utility model provides a convenient nucleic acid extraction element, includes box body (1) and quintuplet pipe (4), establish inside box body (1) quintuplet pipe (4), its characterized in that: an adsorption mechanism is arranged inside the box body (1);

the adsorption device comprises an outer tube (9), the outer tube (9) is arranged inside a box body (1), a fixing rod (12) is arranged inside the outer tube (9), a magnetic rod (13) is fixedly arranged at one end of the fixing rod (12), one end of the fixing rod (12) extends to the top of the outer tube (9), an aluminum tube (14) is inlaid on the outer tube (9), a top cover (10) is arranged on the top end of the box body (1), the box body (1) is connected with the top cover (10) through threads, and the other end of the outer tube (9) extends to the top of the top cover (10) through the top cover (10).

2. A convenient nucleic acid extraction device according to claim 1, wherein: the box body (1) is internally provided with a turntable (2), and the bottom end of the turntable (2) is movably connected with the bottom end inside the box body (1) through a bearing.

3. A convenient nucleic acid extraction device according to claim 2, wherein: the top end of the rotary table (2) is provided with a plurality of placing holes (3) and a water tank (8), the water tank (8) is arranged on the inner side of the placing holes (3), and the five-way pipe (4) is arranged in the placing holes (3).

4. A convenient nucleic acid extraction device according to claim 3, wherein: the bottom end of the placing hole (3) is fixedly provided with a connecting pipe (7), and one end of the connecting pipe (7) is connected with a water tank (8).

5. A convenient nucleic acid extraction device according to claim 2, wherein: the outer end of the rotary table (2) is inlaid with a shifting ring (6), one side of the box body (1) is provided with a groove (5), and the groove (5) is arranged on one side of the shifting ring (6).

6. A convenient nucleic acid extraction device according to claim 1, wherein: one end of the fixed rod (12) is fixedly provided with a finger ring.

7. A convenient nucleic acid extraction device according to claim 1, wherein: top cap (10) bottom mounting is equipped with the rubber circle, the rubber circle inboard contacts with box body (1) outer end, top cap (10) outer end is inlayed and is had sealing washer (11), sealing washer (11) inboard contacts with outer tube (9) outer end.