CN214142344U

CN214142344U - Sampling device for poultry intestinal microorganisms

Info

Publication number: CN214142344U
Application number: CN202023096765.XU
Authority: CN
Inventors: 李莹; 罗成龙; 计坚; 瞿浩; 王艳; 袁裕珊; 程嘉恒; 舒鼎铭
Original assignee: Institute of Animal Science of Guangdong Academy of Agricultural Sciences
Current assignee: Institute of Animal Science of Guangdong Academy of Agricultural Sciences
Priority date: 2020-12-21
Filing date: 2020-12-21
Publication date: 2021-09-07
Anticipated expiration: 2030-12-21

Abstract

The utility model discloses a sampling device of poultry intestinal microorganism, including PP rifle head and liquid nitrogen bin, be provided with the first cut out cut-off rule, second on the PP rifle head and tailor the cut-off rule and the third cuts out the cut-off rule, and the first cut out cut-off rule, second are tailor the cut-off rule and the third is tailor the cut-off rule along the even partition trisection of PP rifle head mouth of pipe department, the one end of PP rifle head after every partition is provided with handheld end, the lower extreme position department in first aseptic centrifuge tube interior is pegged graft and is fixed to PP rifle head after every partition. The utility model discloses an use the rifle head of 1mlPP material as the basis, with the impartial three (just can insert lower part position in 1.5ml centrifuging tube for a short time) of cutting into of rifle head with the scissors, then adorn in 50ml centrifuging tube autoclave sterilization, handheld end during the sampling, with flat mouthful of end sample, choose the intestinal content gently and adorn in 1.5ml aseptic centrifuging tube, arrange the frozen storage of liquid nitrogen in rapidly, possess low cost, disposable, convenient and fast and aseptic effect.

Description

Sampling device for poultry intestinal microorganisms

Technical Field

The utility model relates to an animal intestinal sampling technical field specifically is a sampling device of poultry intestinal microorganism.

Background

The intestinal microorganisms of animals and animals have a mutual-benefit symbiotic relationship, and are closely related to the health condition, energy metabolism, disease development and the like of organisms. A large number of key floras related to growth, energy intake, diarrhea and the like are found in intestinal microorganism research, the content, the composition and the diversity of various intestinal microorganisms are obvious, the caecum microorganisms are rich in content, composition and diversity and are key intestinal sections of the intestinal microorganism research at present, but the caecum microorganisms cannot be sampled in vivo through a sampler, and test animals are generally slaughtered and taken out of the intestinal sections and then intestinal contents are collected to detect the microorganism conditions. Contamination of the sample may occur due to repeated use of the apparatus during slaughtering and sampling.

The existing sampling devices are used for repeated sterilization, cross contamination can also occur, and meanwhile, the manufacturing cost is high, so that the market urgently needs to develop a novel sampling device for poultry intestinal microorganisms to solve the existing problems.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a sampling device of poultry intestinal microorganism to solve the used utensil of current sample that provides in the above-mentioned background art and all repeat the sterilization and use, cross contamination also can appear, the higher problem of cost simultaneously.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a sampling device of poultry intestinal microorganism, includes PP rifle head, autoclave and liquid nitrogen bin, be provided with the first cut-off rule of tailorring, the cut-off rule is tailor to the second and the cut-off rule is tailor to the third on the PP rifle head, and the first cut-off rule of tailorring, the cut-off rule is tailor to the second and the third cuts the cut-off rule evenly divides the triplex along PP rifle head mouth department, the one end of the PP rifle head after every partition is provided with handheld end, the other end of the PP rifle head after every partition is provided with the sample end, the PP rifle head after every partition is pegged graft and is fixed in first aseptic centrifuge tube middle and lower extreme position department, the PP rifle head after every partition is placed in the aseptic centrifuge tube of second.

Preferably, the PP gun head is of a transparent PP pipe material structure.

Preferably, the first sterile centrifuge tube is 1.5ml in size, and a fixed notch is formed in the first sterile centrifuge tube.

Preferably, the second sterile centrifuge tube has a gauge size of 50 ml.

Preferably, the liquid nitrogen storage tank is internally provided with a placing groove, and one end of the liquid nitrogen storage tank is connected with a sealing cover plate through a hinge.

Preferably, the front end of the sealing cover plate is connected with a locking groove arranged on the front end surface of the liquid nitrogen storage tank through a lock catch.

Preferably, there is the autoclave cover one end of autoclave through hinge swing joint, and autoclave's inside is provided with high-pressure inner bag, and high-pressure inner bag's surface is provided with the centrifuging tube fixed slot, and the aseptic centrifuging tube of second has been placed to centrifuging tube fixed slot inside.

Compared with the prior art, the beneficial effects of the utility model are that:

(1) the utility model discloses an on the basis of the rifle head of 1mlPP material, cut the rifle head into three (the size just can insert lower part position in 1.5ml centrifuging tube) equally with the scissors, then adorn in 50ml centrifuging tube autoclave sterilization, handheld end is handed during the sampling, with the sample of flat mouthful end, choose the intestinal content gently and adorn in 1.5ml aseptic centrifuging tube, place in liquid nitrogen cryopreservation rapidly, possess low cost, disposable, convenient and fast and aseptic effect, the sample of intestinal microorganism provides very big facility and cost advantage for poultry;

(2) the liquid nitrogen storage box is internally provided with a placing groove, one end of the liquid nitrogen storage box is connected with a sealing cover plate through a hinge, the front end of the sealing cover plate is connected with a locking groove arranged on the surface of the front end of the liquid nitrogen storage box through a lock catch, the placing groove is used for conveniently fixing and placing a sampled sterile centrifuge tube, and meanwhile, the liquid nitrogen storage box has the advantage of good sealing and low-temperature refrigeration effects;

(3) adopt the autoclave to carry out portable autoclaving to it, possess the fixed convenient of installation, with low costs and convenient to use's advantage.

Drawings

FIG. 1 is a front view of the PP gun head of the present invention;

FIG. 2 is a schematic structural view of the PP gun head of the present invention after being equally divided;

FIG. 3 is a schematic structural view of the PP gun head of the present invention after being fixed with a first aseptic centrifuge tube;

FIG. 4 is a schematic view of the structure of the PP gun head of the present invention placed inside a second sterile centrifuge tube;

FIG. 5 is a schematic view of the internal structure of the liquid nitrogen storage tank of the present invention;

FIG. 6 is a schematic view of the internal structure of the autoclave of the present invention;

FIG. 7 is a top view of the autoclave of the present invention;

fig. 8 is a flowchart of the present invention.

In the figure: 1. PP gun heads; 2. a first cutting dividing line; 3. a second cutting dividing line; 4. thirdly, cutting and dividing lines; 5. a handheld end; 6. a sampling end; 7. a fixed notch; 8. a first sterile centrifuge tube; 9. a second sterile centrifuge tube; 10. locking; 11. sealing the cover plate; 12. a liquid nitrogen storage tank; 13. locking the groove; 14. a hinge; 15. a placement groove; 16. a pressure cooker cover; 17. an autoclave; 18. a centrifuge tube fixing groove; 19. the high-pressure liner.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

Referring to fig. 1-8, the present invention provides an embodiment: the utility model provides a sampling device of poultry intestinal microorganism, including PP rifle head 1, autoclave 17 and liquid nitrogen bin 12, be provided with first cut parting line 2 on the PP rifle head 1, the parting line 3 is tailor to the second and the parting line 4 is tailor to the third, first cut parting line 2, the parting line 3 is tailor to the second and the parting line 4 is tailor to the third evenly divides the trisection along the 1 mouth of pipe department of PP rifle head, the one end of the PP rifle head 1 after every partition is provided with handheld end 5, the other end of the PP rifle head 1 after every partition is provided with sample end 6, the PP rifle head 1 after every partition is pegged graft and is fixed in the inside middle and lower extreme position department of first aseptic centrifuging tube 8, the PP rifle head 1 after every partition is placed in second aseptic centrifuging tube 9.

Further, the PP gun head 1 is of a transparent PP pipe material structure.

Further, the specification and size of first aseptic centrifuging tube 8 is 1.5ml, and first aseptic centrifuging tube 8 is inside to be provided with fixed notch 7.

Further, the second sterile centrifuge tube 9 has a gauge size of 50 ml.

Further, a placement groove 15 is provided inside the liquid nitrogen storage tank 12, and a sealing cover plate 11 is connected to one end of the liquid nitrogen storage tank 12 through a hinge 14.

Further, the front end of the sealing cover plate 11 is connected to a locking groove 13 provided on the front end surface of the liquid nitrogen storage tank 12 through a locking clip 10.

Further, there is high-pressure pot cover 16 one end of autoclave 17 through hinge swing joint, and autoclave 17's inside is provided with high-pressure inner bag 19, and high-pressure inner bag 19's surface is provided with centrifuging tube fixed slot 18, and the aseptic centrifuging tube 9 of second has been placed to centrifuging tube fixed slot 18 inside.

The working principle is as follows: when in use, the PP gun head 1 is provided with a first cutting parting line 2, a second cutting parting line 3 and a third cutting parting line 4, the first cutting parting line 2, the second cutting parting line 3 and the third cutting parting line 4 are evenly divided into three parts along the pipe orifice of the PP gun head 1, the PP gun head 1 is cut and divided into three parts along the first cutting parting line 2, the second cutting parting line 3 and the third cutting parting line 4, the PP gun head 1 after being divided into three parts is spliced and fixed at the middle and lower end positions in a first aseptic centrifuge tube 8 with the specification size of 1.5ml, then the PP gun head is arranged in a second aseptic centrifuge tube 9 with the specification of 50ml for high pressure sterilization, the second aseptic centrifuge tube 9 is inserted and arranged in a centrifuge tube 18, an autoclave 17 is started for high pressure sterilization after an autoclave cover 16 is covered, after the high pressure sterilization treatment, a handheld end 5 is held by a hand during sampling, a flat-opening sampling end 6 is used for sampling, pick the intestinal content gently and adorn in 1.5ml first aseptic centrifuging tube 8, first aseptic centrifuging tube 8 after will taking the sample is placed and is carried out the low temperature preservation in the inside standing groove 15 of liquid nitrogen storage box 12, standing groove 15 is used for conveniently fixing the aseptic centrifuging tube after the sampling and places, this liquid nitrogen storage box 12 possesses the advantage that sealed low temperature refrigeration effect is good simultaneously, whole device possesses the low cost, it is disposable, convenient and fast and aseptic effect, the sample for poultry intestinal microorganism provides very big facility and cost advantage.

It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. A sampling device for poultry intestinal microorganisms comprises a PP gun head (1), an autoclave (17) and a liquid nitrogen storage tank (12), it is characterized in that a first cutting parting line (2), a second cutting parting line (3) and a third cutting parting line (4) are arranged on the PP gun head (1), the first cutting parting line (2), the second cutting parting line (3) and the third cutting parting line (4) are evenly and equally divided into three parts along the pipe orifice of the PP gun head (1), one end of each halved PP gun head (1) is provided with a handheld end (5), the other end of each halved PP gun head (1) is provided with a sampling end (6), each equant PP gun head (1) is fixedly inserted in the middle-lower end position in the first sterile centrifuge tube (8), and each halved PP gun head (1) is placed in a second sterile centrifuge tube (9).

2. The poultry intestinal microorganism sampling device according to claim 1, wherein: the PP gun head (1) is of a transparent PP pipe material structure.

3. The poultry intestinal microorganism sampling device according to claim 1, wherein: the specification and size of the first sterile centrifuge tube (8) are 1.5ml, and a fixed notch (7) is formed in the first sterile centrifuge tube (8).

4. The poultry intestinal microorganism sampling device according to claim 1, wherein: the second sterile centrifuge tube (9) has a specification and size of 50 ml.

5. The poultry intestinal microorganism sampling device according to claim 1, wherein: a placing groove (15) is formed in the liquid nitrogen storage box (12), and one end of the liquid nitrogen storage box (12) is connected with a sealing cover plate (11) through a hinge (14).

6. The poultry intestinal microorganism sampling device according to claim 5, wherein: the front end of the sealing cover plate (11) is connected with a lock groove (13) arranged on the front end surface of the liquid nitrogen storage tank (12) through a lock catch (10).

7. The poultry intestinal microorganism sampling device according to claim 1, wherein: one end of the autoclave (17) is movably connected with an autoclave cover (16) through a hinge, a high-pressure inner container (19) is arranged inside the autoclave (17), a centrifuge tube fixing groove (18) is formed in the surface of the high-pressure inner container (19), and a second sterile centrifuge tube (9) is placed inside the centrifuge tube fixing groove (18).