CN214115545U

CN214115545U - Classical swine fever virus fluorescence PCR detection kit

Info

Publication number: CN214115545U
Application number: CN202023143966.0U
Authority: CN
Inventors: 孙统威
Original assignee: Hangzhou Heng Ao Technology Co ltd
Current assignee: Hangzhou Heng Ao Technology Co ltd
Priority date: 2020-12-23
Filing date: 2020-12-23
Publication date: 2021-09-03
Anticipated expiration: 2030-12-23

Abstract

The utility model provides a classical swine fever virus fluorescence PCR detect reagent box belongs to detect reagent box technical field. The fluorescent PCR detection kit for the classical swine fever virus comprises a kit component. The reagent kit component comprises a kit body, a driving piece, a reagent frame, reagent tubes, a transparent plate and a kit cover, wherein the driving piece comprises a transmission rod, a worm, a rotating rod and a worm wheel, the transmission rod is horizontally and rotatably arranged at the bottom in the kit body, one end of the transmission rod extends out of the kit body, the worm is arranged on one side of the transmission rod, the rotating rod is vertically and rotatably arranged in the kit body, the worm wheel is sleeved on the surface of the rotating rod, the worm wheel and the worm are meshed, the reagent frame and the reagent tubes are driven to rotate through the matching of the transmission rod, the worm, the rotating rod and the worm wheel, and the fluorescence displayed in each reagent tube is observed through the transparent plate, so that the switch of the kit cover is avoided, and the reagent tubes are effectively prevented from being polluted.

Description

Classical swine fever virus fluorescence PCR detection kit

Technical Field

The utility model relates to a detect reagent box field particularly, relates to a swine fever virus fluorescence PCR detect reagent box.

Background

The classical swine fever virus is a pathogen of classical swine fever, and harms pigs and wild pigs, and other animals do not attack the swine fever virus. Hog cholera is an acute, hot and highly contagious infectious disease, and is mainly characterized by systemic hemorrhage, necrosis, infarction and plague bacterial infection caused by high temperature and microvascular degeneration. The swine fever has serious harm to pigs, and can cause great loss in the pig industry. PCR detection methods have been established both abroad and domestically.

At present, need use detect reagent when detecting the classical swine fever virus, but the inconvenient fluorescence of observing of some current kit of depositing the reagent pipe, mostly need open the lid and take out the reagent pipe and observe, lead to the reagent pipe easily to be contaminated.

SUMMERY OF THE UTILITY MODEL

In order to compensate the above deficiency, the utility model provides a classical swine fever virus fluorescence PCR detect reagent box aims at improving current part kit, and inconvenient demonstration fluorescence is not convenient for personnel to carry out the problem of observing.

The utility model discloses a realize like this:

the utility model provides a classical swine fever virus fluorescence PCR detect reagent box includes the reagent box subassembly.

The reagent kit component comprises a kit body, a driving piece, a reagent rack, reagent tubes, a transparent plate and a box cover, wherein the driving piece comprises a transmission rod, a worm, a rotating rod and a worm wheel, the transmission rod is horizontally and rotatably arranged at the bottom in the kit body, one end of the transmission rod extends out of the kit body, the worm is arranged at one side of the transmission rod, the rotating rod is vertically and rotatably arranged in the kit body, the worm wheel is sleeved on the surface of the rotating rod, the worm wheel is meshed with the worm, two reagent racks are arranged, the reagent rack comprises a support rod and a first placing ring, the support rod is provided with a plurality of support rods, the support rod is fixedly connected to the outer side of the rotating rod, the first placing ring is fixedly connected to one end of the support rod, the first placing ring is provided with a jack, the reagent tubes are, the box body top fretwork, the lid rotate set up in box body top fretwork department.

The utility model discloses an in one embodiment, box body top fretwork department is provided with the link, the link includes fixed block and branch, branch is provided with two at least, branch one end fixed connection in the fixed block, branch other end fixed connection in the box body inner wall, bull stick one end rotate connect in the fixed block.

In an embodiment of the present invention, the second placing ring is sleeved on the supporting rod, and the second placing ring has a plurality of inserting holes.

In an embodiment of the present invention, a heat insulation plate is disposed in the box body, and an electric heating plate is disposed on one side of the heat insulation plate.

The utility model discloses an in one embodiment, lid one side is provided with the latch segment, box body one side is provided with the retaining member, the retaining member includes connecting block, spring and kelly, the inner chamber has been seted up to the connecting block, the spring set up in the inner chamber, kelly one end fixed connection in the spring, the kelly other end joint in latch segment one side.

The utility model discloses an in the embodiment, the latch segment is the L type, the card hole has been seted up to latch segment, one side, the kelly with card hole looks adaptation.

The utility model discloses an in one embodiment, the slide opening has been seted up to the inner chamber intercommunication, kelly one side fixedly connected with movable block, the movable block with slide opening inner wall clearance fit.

The utility model discloses an in one embodiment, transfer line one end fixedly connected with knob, the box body outside is seted up flutedly, the knob is located in the recess.

In an embodiment of the present invention, the box body has a top opening, and a handle is disposed in the top opening.

In an embodiment of the present invention, the transmission rod and the worm are designed as an integral structure, and the transparent plate is embedded in one side of the box body.

The utility model has the advantages that: the utility model discloses a pig pestivirus fluorescence PCR detect reagent box that above-mentioned design obtained, during the use, open the lid, to be equipped with the reagent pipe that has handled the solution and insert in the jack, close the lid, when needing to observe fluorescence, the rotation transfer line, the transfer line drives the worm and rotates, the worm meshing drives the worm wheel and rotates, the worm wheel drives the bull stick and rotates, the bull stick drives the reagent frame rotation that is equipped with the reagent pipe, observe one by one through the transparent plate, through the transfer line, the worm, the cooperation of bull stick and worm wheel is used and is driven reagent frame and reagent pipe and rotate, the intraductal fluorescence that demonstrates of every reagent is observed to the rethread transparent plate, thereby avoid the switch of lid, the reagent pipe has been avoided effectively being polluted.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

FIG. 1 is a schematic structural diagram of a fluorescent PCR detection kit for classical swine fever virus according to an embodiment of the present invention;

FIG. 2 is a schematic diagram of a driving member according to an embodiment of the present invention;

FIG. 3 is a schematic diagram of a reagent rack according to an embodiment of the present invention;

fig. 4 is a schematic structural view of a groove provided in an embodiment of the present invention;

fig. 5 is a schematic structural view of a handle according to an embodiment of the present invention;

FIG. 6 is a schematic diagram of a transparent plate according to an embodiment of the present invention;

FIG. 7 is a schematic view of a retaining member according to an embodiment of the present invention;

fig. 8 is a schematic structural view of a locking block according to an embodiment of the present invention.

In the figure: 100-kit components; 110-a cartridge; 111-a connecting frame; 1111-fixed block; 1112-a strut; 112-a heat insulation plate; 1121-electric heating plate; 113-a groove; 114-a limiting groove; 115-a handle; 120-a drive member; 121-a transmission rod; 1211-knob; 122-a worm; 123-rotating rod; 124-worm gear; 130-reagent rack; 131-a support bar; 132-a first placement ring; 1321-jack; 133-a second placement ring; 140-a reagent tube; 150-a transparent plate; 160-box cover; 161-locking block; 1611-card hole; 170-a locking member; 171-connecting block; 1711-lumen; 1712-a slide hole; 172-spring; 173-a clamping rod; 1731-moving block.

Detailed Description

To make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the drawings of the embodiments of the present invention are combined to clearly and completely describe the technical solutions of the embodiments of the present invention, and obviously, the described embodiments are some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In the present disclosure, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact between the first and second features, or may comprise contact between the first and second features not directly. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

Examples

Referring to fig. 1-8, the present invention provides a technical solution: a classical swine fever virus fluorescence PCR detection kit includes kit components 100.

The kit assembly 100 is better able to exhibit fluorescence after detection while avoiding contamination of the reagents.

Referring to fig. 1, 2, 3, 4, 5, 6, 7 and 8, the reagent kit assembly 100 includes a case body 110, a driving member 120, a reagent rack 130, a reagent tube 140, a transparent plate 150 and a case cover 160, the driving member 120 includes a transmission rod 121, a worm 122, a rotation rod 123 and a worm wheel 124, the transmission rod 121 is horizontally and rotatably disposed at the bottom of the case body 110, the transmission rod 121 is horizontally and rotatably disposed at the case body 110 through a bearing, one end of the transmission rod 121 extends out of the case body 110, specifically, one end of the transmission rod 121 is fixedly connected with a knob 1211, the transmission rod 121 is bolted or welded with the knob 1211, a groove 113 is formed at the outer side of the case body 110, the knob 1211 is located in the groove 113, so that a person can manually rotate the transmission rod 121 through the knob 1211, the worm 122 is disposed at one side of the transmission rod 121, the transmission rod 121 and the worm 122 are integrally designed, the rotation rod 123 is vertically and rotatably disposed in the case body 110, specifically, a connection rack 111 is disposed at a hollow portion of the top of the case body 110, the connecting frame 111 comprises a fixing block 1111 and two supporting rods 1112, at least two supporting rods 1112 are arranged, one end of each supporting rod 1112 is fixedly connected to the fixing block 1111, the supporting rod 1112 is bolted or welded to the fixing block 1111, the other end of each supporting rod 1112 is fixedly connected to the inner wall of the box body 110, the supporting rods 1112 are bolted or welded to the box body 110, one end of each rotating rod 123 is rotatably connected to the fixing block 1111 and used for supporting the rotating rod 123 to vertically rotate and keep balance, the worm wheel 124 is sleeved on the surface of the rotating rod 123, the worm wheel 124 is meshed with the worm 122, two reagent racks 130 are arranged, each reagent rack 130 comprises a supporting rod 131 and a first placing ring 132, the supporting rods 131 are provided with a plurality of supporting rods 131, the supporting rods 131 are fixedly connected to the outer sides of the rotating rods 123, the supporting rods 131 are bolted or welded to the rotating rods 123, the first placing rings 132 are fixedly connected to one ends of the supporting rods 131, the first placing rings 132 are bolted or welded to the supporting rods 131, the first placing rings 132 are provided with jacks 1321, reagent tube 140 inserts and locates in jack 1321, specifically, ring 133 is placed to the second that has cup jointed to bracing piece 131, ring 133 has also been placed to the second has seted up a plurality of jacks 1321, it increases reagent tube 140 and places the capacity to set up two and place the ring, two relative cross arrangement of jack 1321 of placing on the ring, avoid reagent tube 140 to block each other and observe the field of vision, in this embodiment, be provided with heat insulating board 112 in the box body 110, heat insulating board 112 one side is provided with electric heating board 1121, be used for heating in the box, increase the practicality, transparent plate 150 sets up in box body 110 one side, specifically, transparent plate 150 inlays in box body 110 one side, box body 110 top fretwork, lid 160 rotates and sets up in box body 110 top fretwork department, lid 160 rotates through the round pin axle and sets up in box body 110, specifically, spacing groove 114 has been.

Wherein, one side of the box cover 160 is provided with a locking block 161, one side of the box body 110 is provided with a locking member 170, the locking member 170 comprises a connecting block 171, a spring 172 and a clamping rod 173, the connecting block 171 is provided with an inner cavity 1711, the spring 172 is arranged in the inner cavity 1711, one end of the clamping rod 173 is fixedly connected to the spring 172, the clamping rod 173 is bolted or welded to the spring 172, the other end of the clamping rod 173 is clamped to one side of the locking block 161, specifically, the locking block 161 is L-shaped, the locking block 161 is provided with a clamping hole 1611 at one side, the clamping rod 173 is matched with the clamping hole 1611, the clamping rod 173 is pushed by the spring 172 to be clamped into the clamping hole 1611, so as to fixedly cover the box cover 160, it should be noted that the inner cavity 1712 is communicated with the sliding hole 1712, one side of the clamping rod 173 is fixedly connected with a moving block 1731, the clamping rod 173 is integrally formed with a moving block 1731, the moving block 1731 is in clearance fit with the inner wall of the sliding hole 1712, so that a human hand can move the moving block 1731 and further move the clamping rod 173 to press the spring 172, the latch rod 173 is moved out of the latch hole 1611, thereby facilitating the opening of the cover 160.

Specifically, the operating principle of the fluorescent PCR detection kit for classical swine fever virus is as follows: when the reagent tube fluorescence analyzer is used, the moving block 1731 is moved, the clamping rod 173 is moved to press the spring 172, the clamping rod 173 is moved out of the clamping hole 1611, the box cover 160 is opened, the reagent tube 140 filled with the processed solution is inserted into the insertion hole 1321, the box cover 160 is closed, the moving block 1731 is loosened, the spring 172 elastically restores to push the clamping rod 173 to enter the clamping hole 1611, so that the box cover 160 is fixedly covered, when fluorescence needs to be observed, the knob 1211 is rotated, the knob 1211 drives the transmission rod 121 to rotate, the transmission rod 121 drives the worm 122 to rotate, the worm 122 is meshed with the worm wheel 124 to drive the worm wheel 124 to rotate, the worm wheel 124 drives the rotating rod 123 to rotate, the rotating rod 123 drives the reagent rack 130 filled with the reagent tube 140 to rotate, the reagent rack 130 and the reagent tube 140 are observed one by one through the transparent plate 150 without opening the box cover 160, the reagent rack 130 and the reagent tube 140 are driven to rotate through the cooperation of the transmission rod 121, the rotating rod 123 and the worm wheel 124, fluorescence displayed in each reagent tube 140 is observed through the transparent plate 150, thereby preventing the opening and closing of the box cover 160 and effectively preventing the reagent tube 140 from being polluted.

It should be noted that the specific model specification of the electric heating plate 1121 needs to be determined by type selection according to the actual specification of the device, and the specific type selection calculation method adopts the prior art, so detailed description is omitted.

The power supply of the electrical heating plate 1121 and the principle thereof will be apparent to those skilled in the art and will not be described in detail herein.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A fluorescent PCR detection kit for classical swine fever viruses is characterized by comprising

The reagent kit component (100) comprises a kit body (110), a driving piece (120), reagent racks (130), reagent tubes (140), a transparent plate (150) and a kit cover (160), wherein the driving piece (120) comprises a transmission rod (121), a worm (122), a rotating rod (123) and a worm wheel (124), the transmission rod (121) is horizontally and rotatably arranged at the bottom in the kit body (110), one end of the transmission rod (121) extends out of the kit body (110), the worm (122) is arranged at one side of the transmission rod (121), the rotating rod (123) is vertically and rotatably arranged in the kit body (110), the worm wheel (124) is sleeved on the surface of the rotating rod (123), the worm wheel (124) is meshed with the worm (122), the reagent racks (130) are provided with two parts, and the reagent racks (130) comprise supporting rods (131) and first placing rings (132), the utility model discloses a reagent tube, including bracing piece (131), rotary rod (123), first ring (132) fixed connection of placing in bracing piece (131) one end, jack (1321) have been seted up in first ring (132) of placing, reagent tube (140) insert locate in jack (1321), transparent plate (150) set up in box body (110) one side, box body (110) top fretwork, lid (160) rotate set up in box body (110) top fretwork department.

2. The fluorescent PCR detection kit for classical swine fever viruses as defined in claim 1, wherein a connecting frame (111) is disposed at the hollow portion of the top of the box body (110), the connecting frame (111) comprises a fixing block (1111) and at least two supporting rods (1112), one end of each supporting rod (1112) is fixedly connected to the fixing block (1111), the other end of each supporting rod (1112) is fixedly connected to the inner wall of the box body (110), and one end of each rotating rod (123) is rotatably connected to the inside of the fixing block (1111).

3. The fluorescent PCR detection kit for classical swine fever viruses as defined in claim 1, wherein a second placing ring (133) is sleeved on the support rod (131), and a plurality of the insertion holes (1321) are formed in the second placing ring (133).

4. The fluorescent PCR detection kit for classical swine fever viruses as defined in claim 1, wherein a heat insulation board (112) is disposed in the kit body (110), and an electric heating plate (1121) is disposed on one side of the heat insulation board (112).

5. The fluorescent PCR detection kit for classical swine fever viruses according to claim 1, wherein a locking block (161) is disposed at one side of the box cover (160), a locking member (170) is disposed at one side of the box body (110), the locking member (170) comprises a connecting block (171), a spring (172) and a clamping rod (173), an inner cavity (1711) is formed in the connecting block (171), the spring (172) is disposed in the inner cavity (1711), one end of the clamping rod (173) is fixedly connected to the spring (172), and the other end of the clamping rod (173) is clamped at one side of the locking block (161).

6. The fluorescent PCR detection kit for classical swine fever viruses as defined in claim 5, wherein the locking block (161) is L-shaped, a locking hole (1611) is formed on one side of the locking block (161), and the locking rod (173) is adapted to the locking hole (1611).

7. The fluorescent PCR detection kit for classical swine fever viruses according to claim 6, wherein the inner cavity (1711) is communicated with a slide hole (1712), a moving block (1731) is fixedly connected to one side of the clamping rod (173), and the moving block (1731) is in clearance fit with the inner wall of the slide hole (1712).

8. The fluorescent PCR detection kit for classical swine fever viruses as defined in claim 1, wherein a knob (1211) is fixedly connected to one end of the transmission rod (121), a groove (113) is formed in the outer side of the box body (110), and the knob (1211) is located in the groove (113).

9. The fluorescent PCR detection kit for classical swine fever viruses as defined in claim 1, wherein a limiting groove (114) is formed at the top of the box body (110), and a handle (115) is disposed in the limiting groove (114).

10. The fluorescent PCR detection kit for classical swine fever viruses as defined in claim 1, wherein the transmission rod (121) and the worm (122) are integrally designed, and the transparent plate (150) is embedded on one side of the box body (110).