CN214060527U

CN214060527U - Ultra-large biological macromolecule extraction device

Info

Publication number: CN214060527U
Application number: CN202023094031.8U
Authority: CN
Inventors: 杨锦宇
Original assignee: Haining Boshang Biotechnology Co ltd
Current assignee: Haining Boshang Biotechnology Co ltd
Priority date: 2020-12-21
Filing date: 2020-12-21
Publication date: 2021-08-27
Anticipated expiration: 2030-12-21

Abstract

The utility model discloses a biomacromolecule extraction element in a large number relates to the biomacromolecule and draws equipment technical field, to the not good problem of current extraction element working property, now proposes following scheme, and it is including drawing the casing, the top movable sleeve that draws the casing is equipped with the top cap, the silo of throwing has all been seted up at the both ends of top cap, the inner wall of top cap bottom is fixed with an installation section of thick bamboo, the inner wall that draws the casing bottom has seted up and has loaded the groove, the bottom sliding connection of an installation section of thick bamboo is in the inside of loading the groove, the inner wall at installation section of thick bamboo top is fixed with driving motor, driving motor's output transmission is connected with the drive shaft. The utility model discloses can carry out quick mixing to magnetic bead and washing liquid and handle, and can carry out the separation to the magnetic bead, avoid magnetic bead and pivoted stirring part to take place rigid collision to can be simple and convenient dismantle the maintenance to extraction element's stirring part, the working property is high, convenient to use.

Description

Ultra-large biological macromolecule extraction device

Technical Field

The utility model relates to a biomacromolecule draws equipment technical field, especially relates to a super large amount of biomacromolecule extraction element.

Background

The existing biomacromolecule extraction method is mostly a magnetic bead method, because the method is convenient and easy to realize automation, more and more use scenes are provided, the nucleic acid extraction of a column method is basically eliminated at present, along with the use of various devices, the high-flux nucleic acid extraction is rapidly developed at present, the patterns are different, but the basic operation steps are still adsorption, washing and elution. The equipment has perfect extraction quality and flux, and in the process of extracting biomacromolecules, a corresponding extraction device is needed to uniformly mix and stir magnetic beads and washing liquid so as to achieve the purpose of washing, thereby facilitating a user to carry out the next step of elution treatment.

The existing extraction device is mostly provided with a corresponding stirring component, the magnetic beads and the washing liquid are uniformly mixed through the stirring component, the magnetic beads and the washing liquid can be uniformly mixed, but in the using process, the stirring component of the extraction device is mostly in direct contact with the magnetic beads inside the extraction device, therefore, the stirring component of the extraction device is very easy to collide with the magnetic beads in the operation process, in the process, the damage of the magnetic beads and the stirring component is very easy to cause, the service lives of the magnetic beads and the stirring component are greatly reduced, the working performance is poor, and therefore an ultra-large quantity of biomacromolecule extraction devices are provided.

SUMMERY OF THE UTILITY MODEL

The utility model provides a super a large amount of biomacromolecule extraction element has solved the not good problem of extraction element working property.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a huge amount of biomacromolecule extraction device comprises an extraction shell, wherein a top cover is movably sleeved at the top of the extraction shell, feed slots are formed in two ends of the top cover, an installation cylinder is fixed on the inner wall of the bottom of the top cover, a loading slot is formed in the inner wall of the bottom of the extraction shell, the bottom of the installation cylinder is slidably connected inside the loading slot, a driving motor is fixed on the inner wall of the top of the installation cylinder, the output end of the driving motor is in transmission connection with a driving shaft, one end, away from the driving motor, of the driving shaft is rotatably connected on the inner wall of the bottom of the installation cylinder through a bearing, a plurality of stirring rods are vertically fixed on the outer wall of the driving shaft, the stirring rods are rotatably sleeved inside the installation cylinder, a plurality of water permeable holes are formed in the outer wall of the installation cylinder, a filter screen is fixed on the inner wall of the bottom of the extraction shell, and a connecting hole is formed in the middle part of the filter screen, the bottom sliding connection of installation section of thick bamboo is in the inside of connecting hole, draw the bottom of casing one side and install drainage tube, the last butterfly valve that installs of drainage tube.

Preferably, the top of installation section of thick bamboo both sides all is fixed with loads the piece, and the outer wall that should load the piece all articulates through the hinge has the dog, throw the silo and seal through corresponding dog respectively, the one end that the silo is close to driving motor of throwing all is equipped with the holding tank, the holding tank is all seted up on the inner wall at top cap top, the inner wall at holding tank top all is fixed with a plurality of coupling springs, the outside of holding tank is all extended to coupling spring's the other end to fix respectively at corresponding dog top.

Preferably, the top of drawing the casing both sides has all been seted up the mounting groove, the equal sliding connection in inside of mounting groove has the slider, the inner wall of mounting groove all is fixed with a plurality of reset spring, reset spring's the other end is fixed respectively on corresponding slider, one side that reset spring was kept away from to the slider all is fixed with the fixture block, the draw-in groove has all been seted up at the both ends of top cap, the draw-in groove is corresponding with the mounting groove, the one end that the slider was kept away from to the fixture block extends the inside of mounting groove to difference sliding connection is inside corresponding draw-in groove.

Preferably, one side of the outer part of the installation cylinder is hinged with a movable door through a hinge, and a handle is fixed on the outer side of the movable door.

Preferably, the extraction shell is a box body with an upward opening, and the top cover is of a concave structure.

Preferably, the depth of the mounting groove is greater than the length of the fixture block, and the fixture block is slidably connected with the mounting groove.

The utility model discloses in:

through the matching work of the extraction shell, the top cover, the feeding groove, the mounting cylinder, the loading groove, the driving motor, the driving shaft, the stirring rod, the water permeable hole, the filter screen, the connecting hole, the liquid discharge pipeline, the stop block, the holding groove, the connecting spring, the mounting groove, the slide block, the reset spring, the clamping block, the clamping groove and the movable door, on one hand, the magnetic beads and the washing liquid can be quickly mixed and treated, and when the magnetic beads and the washing liquid are mixed and treated, the magnetic beads are blocked, the hard collision between the magnetic beads and the rotating stirring part is avoided, and then the service lives of the magnetic beads and the stirring part are effectively prolonged; on the other hand can seal extraction element's feed inlet to when avoiding magnetic bead and washing liquid to stir the mixing in extraction element's inside, washing liquid is from extraction element spill, then effectual improvement extraction element's working property, and can be according to user's maintenance demand, simple and convenient dismantles the maintenance, convenient to use to extraction element's stirring part.

To sum up, the utility model discloses can carry out quick mixing to magnetic bead and washing liquid and handle, and can carry out the separation to the magnetic bead, avoid magnetic bead and pivoted stirring part to take place rigid collision to can be simple and convenient dismantle the maintenance to extraction element's stirring part, the working property is high, convenient to use.

Drawings

FIG. 1 is a schematic structural diagram of an ultra-large biomacromolecule extraction device provided by the present invention;

FIG. 2 is an enlarged view of a portion A of FIG. 1;

FIG. 3 is an enlarged view of a portion B of FIG. 1;

FIG. 4 is a front view of the installation cylinder of the huge number of biomacromolecule extraction device provided by the present invention.

Reference numbers in the figures: 1. extracting the shell; 2. a top cover; 3. a feeding trough; 4. mounting the cylinder; 5. a loading slot; 6. a drive motor; 7. a drive shaft; 8. a stirring rod; 9. water permeable holes; 10. filtering with a screen; 11. connecting holes; 12. a liquid discharge conduit; 13. a stopper; 14. accommodating grooves; 15. a connecting spring; 16. mounting grooves; 17. a slider; 18. a return spring; 19. a clamping block; 20. a card slot; 21. a movable door.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

Referring to fig. 1-4, a huge amount of biomacromolecule extraction device comprises an extraction shell 1, a top cover 2 is movably sleeved on the top of the extraction shell 1, feeding grooves 3 are respectively arranged at two ends of the top cover 2, an installation cylinder 4 is fixed on the inner wall of the bottom of the top cover 2, a loading groove 5 is arranged on the inner wall of the bottom of the extraction shell 1, the bottom of the installation cylinder 4 is slidably connected inside the loading groove 5, a driving motor 6 is fixed on the inner wall of the top of the installation cylinder 4, the output end of the driving motor 6 is in transmission connection with a driving shaft 7, one end of the driving shaft 7, far away from the driving motor 6, is rotatably connected on the inner wall of the bottom of the installation cylinder 4 through a bearing, a plurality of stirring rods 8 are vertically fixed on the outer wall of the driving shaft 7, the stirring rods 8 are rotatably sleeved inside the installation cylinder 4, a plurality of water permeable holes 9 are arranged on the outer wall of the installation cylinder 4, and a filter screen 10 is fixed on the inner wall of the bottom of the extraction shell 1, the aperture of the permeable hole 9 and the aperture of the filter screen 10 are both smaller than the diameter of a magnetic bead, the middle part of the filter screen 10 is provided with a connecting hole 11, the bottom of the mounting cylinder 4 is slidably connected inside the connecting hole 11, the bottom of one side of the extraction shell 1 is provided with a drainage pipeline 12, the drainage pipeline 12 is provided with a butterfly valve, the tops of two sides of the mounting cylinder 4 are both fixed with a loading block, the outer wall of the loading block is hinged with a stop block 13 through a hinge, the feeding grooves 3 are respectively sealed through corresponding stop blocks 13, one ends of the feeding grooves 3 close to the driving motor 6 are both provided with accommodating grooves 14, the accommodating grooves 14 are both arranged on the inner wall of the top cover 2, the inner wall of the top of the accommodating grooves 14 is both fixed with a plurality of connecting springs 15, the other ends of the connecting springs 15 all extend out of the accommodating grooves 14 and are respectively fixed on the tops of the corresponding stop blocks 13, when a user extracts biomacromolecules, when the extraction device is required to be used for stirring and uniformly mixing the magnetic nano material and the washing liquid, the user can push the stopper 13 at the moment, the stopper 13 can be driven to deflect through the hinged cooperation of the stopper 13 and the loading block at the moment, after the feeding groove 3 is opened by the stopper 13, the user can feed sufficient magnetic beads and washing liquid into the extraction shell 1 through the feeding groove 3 at the moment, the magnetic beads entering the extraction shell 1 can fall on the top of the filter screen 10 and can bear the magnetic beads through the filter screen 10, after the sufficient magnetic beads and the washing liquid enter the extraction shell 1, the stopper 13 can be loosened by the user at the moment, the stopper 13 can be driven to reset through the resetting elasticity of the connecting spring 15 at the moment, the stopper 13 is driven to reset through the hinged cooperation of the stopper 13 and the loading block, the reset stopper 13 can seal the feeding groove 3 at the moment, thereby avoiding the washing liquid from splashing out of the feeding groove 3 when the magnetic beads and the washing liquid are stirred and mixed uniformly in the extraction shell 1, further effectively improving the working performance of the extraction device, and when the baffle block 13 seals the feeding groove 3, the driving shaft 7 can be driven to rotate by the operation of the driving motor 6, the stirring rod 8 can be driven to rotate by the rotating driving shaft 7, the washing liquid in the extracting shell 1 can be driven to rotate by the rotating stirring rod 8, thereby fully reacting with the magnetic beads on the top of the filter screen 10, and when the magnetic beads are uniformly mixed with the washing liquid, the magnetic beads can be blocked by the mounting cylinder 4, thereby avoid the magnetic bead to collide with 8 of pivoted stirring rod, effectual improvement magnetic bead and 8 life of stirring rod then.

The top of the two sides of the extracting shell 1 is provided with a mounting groove 16, the inside of the mounting groove 16 is connected with a sliding block 17 in a sliding manner, the inner wall of the mounting groove 16 is fixed with a plurality of reset springs 18, the other ends of the reset springs 18 are respectively fixed on the corresponding sliding blocks 17, one side of the sliding block 17 far away from the reset springs 18 is fixed with a clamping block 19, the two ends of the top cover 2 are respectively provided with a clamping groove 20, the clamping grooves 20 correspond to the mounting groove 16, one end of the clamping block 19 far away from the sliding block 17 extends out of the inside of the mounting groove 16 and is respectively connected in the corresponding clamping grooves 20 in a sliding manner, one side of the outside of the mounting barrel 4 is hinged with a movable door 21 through a hinge, the outside of the movable door 21 is fixed with a handle, and after the extracting device works for a long time, when a user needs to disassemble and maintain a stirring part of the extracting device, the user can press the clamping block 19 at the time, and can work through the sliding fit of the clamping block 19 and the clamping groove 20 at the time, through the sliding fit work of mounting groove 16 and slider 17, drive fixture block 19 and carry out the displacement, break away from the inside of draw-in groove 20 when fixture block 19, enter into the inside back of mounting groove 16, the user can pull top cap 2 this moment, accessible top cap 2 and the sliding fit work who draws casing 1 this moment, drive top cap 2 carries out the displacement, the sliding fit work of accessible installation section of thick bamboo 4 and loading chute 5 this moment, drive installation section of thick bamboo 4 and carry out the displacement, after the inside of drawing casing 1 is broken away from to installation section of thick bamboo 4, the dodge gate 21 can be opened to the user this moment, the user can be convenient this moment to the inside driving motor 6 of installation section of thick bamboo 4, drive shaft 7 and stirring rod 8 overhaul the maintenance, high durability and convenient use.

Draw casing 1 and be the ascending box of opening, top cap 2 is the spill structure, and the degree of depth of mounting groove 16 is greater than fixture block 19's length,

fixture block

19 and 16 sliding connection of mounting groove, through the mounting groove 16 that the degree of depth is greater than fixture block 19 length, can carry out effectual accomodating to fixture block 19.

The working principle is as follows: when a user needs to use the extraction device to stir and mix the magnetic nano material and the washing liquid uniformly in the process of extracting the biomacromolecules, the user can push the stopper 13 at the moment, the stopper 13 can be driven to deflect through the hinged cooperation of the stopper 13 and the loading block at the moment, after the stopper 13 is opened to the feeding groove 3, the user can feed sufficient magnetic beads and washing liquid into the extraction shell 1 through the feeding groove 3 at the moment, the magnetic beads entering the extraction shell 1 can fall on the top of the filter screen 10, the magnetic beads are supported through the filter screen 10, after the sufficient magnetic beads and washing liquid enter the extraction shell 1, the stopper 13 can be loosened by the user at the moment, the reset elasticity of the connecting spring 15 can be realized at the moment, the stopper 13 is driven to reset through the hinged cooperation of the stopper 13 and the loading block, the reset stopper 13 can seal the feeding groove 3 at the moment, thereby avoiding the magnetic beads and the washing liquid from splashing out of the feeding groove 3 when the feeding groove 3 is sealed by the stopper 13, effectively improving the working performance of the extracting device, driving the driving shaft 7 to rotate by the operation of the driving motor 6 when the feeding groove 3 is sealed by the stopper 13, driving the stirring rod 8 to rotate by the rotating driving shaft 7, driving the washing liquid in the extracting shell 1 by the rotating stirring rod 8, driving the washing liquid in the extracting shell 1 to rotate, fully reacting with the magnetic beads on the top of the filter screen 10, separating the magnetic beads by the mounting cylinder 4 when the magnetic beads and the washing liquid are mixed, avoiding the collision between the magnetic beads and the rotating stirring rod 8, effectively improving the service life of the magnetic beads and the stirring rod 8, and after the extracting device works for a long time, when a user needs to disassemble and maintain the stirring component of the extraction device, the user can press the fixture block 19 at the moment, and the fixture block 19 and the clamping groove 20 can work in a sliding fit manner, the slide fit of the mounting groove 16 and the slide block 17 drives the clamping block 19 to displace, when the clamping block 19 is separated from the inside of the clamping groove 20 and enters the inside of the mounting groove 16, the user can pull the top cover 2, the top cover 2 can be driven to displace through the sliding fit work of the top cover 2 and the extraction shell 1, the mounting cylinder 4 can be driven to displace through the sliding fit work of the mounting cylinder 4 and the loading groove 5, after installation section of thick bamboo 4 and the inside that breaks away from and draw casing 1, the user can open dodge gate 21 this moment, and the user can be convenient overhauls the inside driving motor 6, drive shaft 7 and the puddler 8 of installation section of thick bamboo 4 and maintains convenient to use this moment.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. The ultra-large biomacromolecule extraction device comprises an extraction shell (1) and is characterized in that a top cover (2) is movably sleeved at the top of the extraction shell (1), feed chutes (3) are formed in two ends of the top cover (2), an installation cylinder (4) is fixed on the inner wall of the bottom of the top cover (2), a loading groove (5) is formed in the inner wall of the bottom of the extraction shell (1), the bottom of the installation cylinder (4) is slidably connected inside the loading groove (5), a driving motor (6) is fixed on the inner wall of the top of the installation cylinder (4), a driving shaft (7) is connected to the output end of the driving motor (6) in a transmission mode, one end, far away from the driving motor (6), of the driving shaft (7) is rotatably connected to the inner wall of the bottom of the installation cylinder (4) through a bearing, a plurality of stirring rods (8) are vertically fixed on the outer wall of the driving shaft (7), stirring rod (8) rotate the cover and establish the inside at installation section of thick bamboo (4), a plurality of holes (9) of permeating water have been seted up to the outer wall of installation section of thick bamboo (4), the inner wall that draws casing (1) bottom is fixed with filter screen (10), connecting hole (11) have been seted up at the middle part of filter screen (10), the bottom sliding connection of installation section of thick bamboo (4) is in the inside of connecting hole (11), draw the bottom of casing (1) one side and install drainage tube (12), install the butterfly valve on drainage tube (12).

2. The extraction device for the huge amount of biological macromolecules of claim 1, wherein the top of the two sides of the installation cylinder (4) is fixed with a loading block, the outer wall of the loading block is hinged with a stop block (13) through a hinge, the feeding grooves (3) are respectively sealed through the corresponding stop blocks (13), one ends of the feeding grooves (3) close to the driving motor (6) are respectively provided with a containing groove (14), the containing grooves (14) are respectively arranged on the inner wall of the top cover (2), the inner wall of the top of the containing groove (14) is respectively fixed with a plurality of connecting springs (15), and the other ends of the connecting springs (15) extend out of the containing groove (14) and are respectively fixed on the top of the corresponding stop blocks (13).

3. The extraction device for the ultra-large number of biological macromolecules as claimed in claim 1, wherein mounting grooves (16) are formed in the tops of two sides of the extraction shell (1), sliders (17) are slidably connected to the inside of the mounting grooves (16), a plurality of return springs (18) are fixed to the inner walls of the mounting grooves (16), the other ends of the return springs (18) are respectively fixed to the corresponding sliders (17), clamping blocks (19) are fixed to one sides, away from the return springs (18), of the sliders (17), clamping grooves (20) are formed in two ends of the top cover (2), the clamping grooves (20) correspond to the mounting grooves (16), one ends, away from the sliders (17), of the clamping blocks (19) extend out of the inside of the mounting grooves (16), and are respectively and slidably connected to the inside of the corresponding clamping grooves (20).

4. The extraction apparatus for huge amount of biological macromolecules of claim 1, wherein the installation cylinder (4) is hinged with a movable door (21) on one side of the outside through a hinge, and a handle is fixed on the outside of the movable door (21).

5. An extraction apparatus for very large numbers of biological macromolecules as claimed in claim 1, wherein the extraction housing (1) is a box with an upward opening and the top cover (2) is a concave structure.

6. The extraction apparatus for huge amount of biological macromolecules of claim 3, wherein the depth of the installation groove (16) is larger than the length of the fixture block (19), and the fixture block (19) is connected with the installation groove (16) in a sliding way.