CN214041862U

CN214041862U - Microscopic imaging system

Info

Publication number: CN214041862U
Application number: CN202023203961.2U
Authority: CN
Inventors: 张晓佳
Original assignee: Shanghai Ruiyu Biotech Co Ltd
Current assignee: Shanghai Ruiyu Biotech Co Ltd
Priority date: 2020-12-25
Filing date: 2020-12-25
Publication date: 2021-08-24
Anticipated expiration: 2030-12-25

Abstract

The embodiment of the utility model provides a microscopic imaging system, microscopic imaging system include light source, formation of image objective, objective table and imaging unit, light source provides the illuminating beam, the objective table is used for bearing the weight of the orifice plate, after illuminating beam throws the sample on the orifice plate, the sample is imaged in imaging unit by the formation of image objective; the imaging device further comprises a field diaphragm which is positioned on an optical path between the imaging objective lens and the imaging unit. The embodiment of the utility model provides a microscopic imaging system to can carry out the selectivity to the light beam after imaging objective assembles and shelter from, shelter from and get rid of stray light, eliminate stray halo and vignetting that the concave liquid level edge leads to, improve the imaging quality.

Description

Microscopic imaging system

Technical Field

The utility model relates to a microscopic imaging technique especially relates to a microscopic imaging system.

Background

The microscope is used as a microcosmic observation device and is more and more widely applied to the fields of scientific research, industry and the like. The conventional microscope light source consists of a condenser and an aperture, and has the function of concentrating light rays on a specimen to be observed. When the optical objective lens is used for imaging a 96-well plate mainly used for biological culture, a concave liquid surface is formed due to the surface tension of liquid, and a light source irradiates the edge of the well, so that stray light halo and dark corners exist during imaging, and the imaging quality is poor. And if the flux of the bright field light source is increased, bright spots are formed in the center.

SUMMERY OF THE UTILITY MODEL

The embodiment of the utility model provides a microscopic imaging system to can carry out the selectivity to the light beam after imaging objective assembles and shelter from, shelter from and get rid of stray light, eliminate stray halo and vignetting that the concave liquid level edge leads to, improve the imaging quality.

The embodiment of the utility model provides a microscopic imaging system, including illumination source, formation of image objective, objective table and imaging element, illumination source provides the illuminating beam, the objective table is used for bearing the weight of the orifice plate, after the illuminating beam throws the sample on the orifice plate, the sample is imaged on the imaging element by the formation of image objective;

the imaging device further comprises a field diaphragm which is positioned on an optical path between the imaging objective lens and the imaging unit.

Optionally, the sample is imaged at the imaging unit to comprise a central region and a peripheral region, the peripheral region surrounding the central region,

when the central area is imaged, the diameter of the light through hole of the field diaphragm is D1, and when the peripheral area is imaged, the diameter of the light through hole of the field diaphragm is D2, and D1 is less than D2.

Optionally, the exit pupil diameter of the imaging objective lens is H, and the target surface size of the imaging unit is K;

when H/2 is more than or equal to K, D1 is more than or equal to H/5 and less than or equal to K/4;

when H/5 > K/2, D1 is equal to K/2.

when H/2 is less than K, D2 is equal to K;

when H/3 is greater than K, H/3 is greater than or equal to D2 is greater than or equal to H/2.

Optionally, the central region is a cross.

Optionally, the diaphragm comprises a plectrum disc, a shading scribing sheet, a guide rod and a driver;

the plurality of shading scribing sheets are arranged on the sheet shifting disc and connected with one end of the guide rod, and the other end of the guide rod is connected with the driver; the driver drives the guide rod to move, and the diameter of the light through hole formed by polymerization of the shading scribing slices is controlled.

Optionally, the field stop includes a stop plate and a plurality of light passing holes disposed on the stop plate;

the plurality of light through holes comprise a first light through hole and a second light through hole, and the diameter of the first light through hole is smaller than that of the second light through hole.

Optionally, the imaging device further comprises a tube mirror, and the tube mirror is located on a light path between the field stop and the imaging unit.

Optionally, the illumination light source includes a lamp bead, a first lens, a second lens, a third lens, a bright field diaphragm, a fourth lens, and a fifth lens, which are sequentially arranged along an optical axis.

Optionally, the imaging objective has a magnification of 10 times.

The embodiment of the utility model provides a pair of micro-imaging system sets up the field diaphragm between formation of image objective and imaging unit, and the field diaphragm is located behind the formation of image objective to can carry out the selectivity to the light beam after assembling formation of image objective and shelter from and get rid of stray light, eliminate stray halo and the vignetting that the concave liquid level edge leads to, improve the imaging quality.

Drawings

Fig. 1 is a schematic structural diagram of a microscopic imaging system according to an embodiment of the present invention;

FIG. 2 is a schematic view of the sample on the well plate in the area imaged by the imaging unit;

fig. 3 is a schematic structural diagram of a field stop according to an embodiment of the present invention;

fig. 4 is a schematic structural diagram of another field stop according to an embodiment of the present invention;

fig. 5 is a schematic structural diagram of an illumination source according to an embodiment of the present invention.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings and examples. It is to be understood that the specific embodiments described herein are merely illustrative of the invention and are not limiting of the invention. It should be further noted that, for the convenience of description, only some of the structures related to the present invention are shown in the drawings, not all of the structures.

Fig. 1 is the embodiment of the utility model provides a structural schematic diagram of a microscopic imaging system, refer to fig. 1, microscopic imaging system includes illumination source 41, formation of image objective 43, objective table 42 and imaging unit 45, and illumination source 41 provides the illuminating beam, and objective table 42 is used for bearing the orifice plate, and after illuminating beam throwed the sample on the orifice plate, the sample was imaged in imaging unit 45 by imaging objective 43. The microscopic imaging system further comprises a field stop 40, the field stop 40 being located in the optical path between the imaging objective 43 and the imaging unit 45.

Exemplarily, referring to fig. 1, the stage 42 is located between the illumination light source 41 and the imaging objective 43, and the imaging objective 43 is located between the stage 42 and the imaging unit 45.

The embodiment of the utility model provides a pair of micro-imaging system sets up field stop 40 between formation of image objective 43 and imaging unit 45, and field stop 40 is located behind formation of image objective 43 to can carry out the selectivity to the light beam after assembling formation of image objective 43 and shelter from, shelter from and get rid of stray light, eliminate stray halo and the vignetting that the concave liquid level edge leads to, improve the imaging quality. Compare in setting up the field stop between illumination source and formation of image objective, the embodiment of the utility model provides a set up the field stop in the light path between formation of image objective and the imaging unit under the same illumination source intensity, can not reduce the light flux volume, consequently can not change imaging system's optical resolution.

Fig. 2 is a schematic diagram of an imaging area formed by the imaging unit of a sample on the well plate, and referring to fig. 1 and 2, after an illumination beam provided by an illumination light source 41 is projected onto a stage 42, the illumination beam is projected onto the sample on the well plate carried by the stage 42, then the light projected onto the sample is converged by an imaging objective lens 43, and after being filtered by a field stop 40, the sample is imaged on an imaging unit 45, and an image is formed on the imaging unit 45. Specifically, the sample placed in the well plate (illustratively, the well plate is a 96-well plate, and 96 samples can be placed at the maximum) is imaged by the imaging unit 45 to include a central region 451 and a peripheral region 452, and the peripheral region 452 surrounds the central region 451. When the central area 451 is imaged, the diameter of the light passing hole 401 of the field stop 40 is D1. In the imaging of the peripheral region 452, the diameter of the light passage hole 401 of the field stop 40 is D2, D1 < D2. The embodiment of the utility model provides an in, when central zone 451 imaged, the logical unthreaded hole 401 of field stop 40 was less, and when peripheral zone 452 imaged, the logical unthreaded hole 401 of field stop 40 was great, through the size that changes the logical unthreaded hole 401 of field stop 40, adapted to the formation of image in different regions to have the same luminance when making central zone and peripheral zone formation of image, improved the imaging quality.

Illustratively, referring to fig. 1-2, the image of the sample on the well plate formed at the imaging unit 45 includes a plurality of sub-regions. One sub-region corresponds to a single-hole sample picture obtained by photographing once, and the number of the sub-regions is related to the aperture D of the hole plate and the target surface size K of the imaging unit 45. Taking an aperture of a 96-well plate of 6.4mm and a target surface size K of the imaging unit 45 of 1.3mm as an example, the number of sub-regions is D/K rounded, i.e. 25. As such, the plurality of sub-regions are a first sub-region Q1, a second sub-region Q2, a third sub-region Q3, a fourth sub-region Q4, a fifth sub-region Q5, a sixth sub-region Q6, a seventh sub-region Q7, an eighth sub-region Q8, a ninth sub-region Q9, a tenth sub-region Q10, an eleventh sub-region Q11, a twelfth sub-region Q12, a thirteenth sub-region Q13, a fourteenth sub-region Q14, a fifteenth sub-region Q15, a sixteenth sub-region Q16, a seventeenth sub-region Q17, an eighteenth sub-region Q18, a nineteenth sub-region Q19, a twentieth sub-region Q20, a twenty-first sub-region Q21, a twenty-second sub-region Q22, a twenty-third sub-region Q23, a twenty-fourth sub-region Q24, and a twenty-fifth sub-region Q25, respectively. The first to fifth sub-regions Q1 to Q5 are arranged in a line, the sixth to tenth sub-regions Q6 to Q10 are arranged in a line, the eleventh to fifteenth sub-regions Q11 to Q15 are arranged in a line, the sixteenth to twentieth sub-regions Q16 to Q20 are arranged in a line, and the twenty-first to twenty-fifth sub-regions Q21 to Q25 are arranged in a line. The first to twenty-first sub-regions Q1 to Q21 are arranged in a row. The central region 451 includes an eighth sub-region Q8, a thirteenth sub-region Q13, an eighteenth sub-region Q18, a twelfth sub-region Q12, and a fourteenth sub-region Q14. The peripheral region 452 includes other sub-regions outside the central region 451, which will not be described in detail herein. In the embodiment of the present invention, in order to shoot the image of the sample in the complete single hole of the hole plate, a mode of fusing 25 photos (i.e. 25 sub-regions) is used.

Illustratively, the sample shots in a single well of the well plate may be sequentially imaged in the order of the first sub-region Q1 to the twenty-fifth sub-region Q25. In other embodiments, the plurality of sub-regions may be arranged differently from the plurality of sub-regions shown in fig. 2, for example, the first to fifth sub-regions Q1 to Q5 are arranged in a column, the sixth to tenth sub-regions Q6 to Q10 are arranged in a column, the eleventh to fifteenth sub-regions Q11 to Q15 are arranged in a column, the sixteenth to twentieth sub-regions Q16 to Q20 are arranged in a column, and the twenty-first to twenty-fifth sub-regions Q21 to Q25 are arranged in a column. The first sub-region Q1, the tenth sub-region Q10, the twentieth sub-region Q20 and the twenty-first sub-region Q21 are arranged in a row.

Alternatively, referring to fig. 1-2, the central region 451 is a cross. The central region 451 and the peripheral region 452 together form a square region, with the central region 451 being located at the center of the peripheral region 452. The embodiment of the utility model provides an in, the orifice plate can be 96 orifice plates, and the sample on the orifice plate forms 25 subregions after imaging unit 45 shoots 25 times, and the shape that a plurality of subregions that are located central zone 451 occupy is the cross.

The diameter of the clear aperture 401 of the field stop 40 can be varied in different ways, and different clear aperture diameters can be used to image different areas of the sample in a single aperture of the aperture plate. Alternatively, referring to fig. 3, the field stop 40 includes a dial 402, a light-shielding scribe 403, a guide bar 404, and a driver 405. A plurality of light-shielding slits 403 are provided on the dial 402, the plurality of light-shielding slits 403 are connected to one end of a guide rod 404, and the other end of the guide rod 404 is connected to a driver 405. The driver 405 drives the guide rod 404 to move, and controls the diameter of the light through hole 401 formed by the aggregation of the plurality of light shielding slits 403. The embodiment of the utility model provides an in, the diameter size of the clear aperture 401 of the diaphragm 40 of visual field of change automatically through driver 405, when central zone 451 formed images, the diameter of the clear aperture 401 of the diaphragm 40 of control visual field of view was D1. When imaging the peripheral region 452, the diameter of the light transmission hole 401 of the field stop 40 is controlled to be D2, D1 < D2.

Illustratively, referring to fig. 3, the plurality of light-shielding scribes 403 collectively shield light, and a light-passing hole 401 is formed at a central position where the plurality of light-shielding scribes 403 converge, and the diameter size of the light-passing hole 401 can be controlled by the movement of the plurality of light-shielding scribes 403.

Alternatively, the exit pupil diameter of the imaging objective 43 is H and the target surface size of the imaging unit 45 is K. The target surface size of the imaging unit 45 refers to the maximum distance in the transverse direction or the longitudinal direction in the target surface of the imaging unit 45, for example, the target surface of the imaging unit 45 is rectangular, and the target surface size of the imaging unit 45 is the length of the maximum side length of the rectangle. When H/2 is more than or equal to K, D1 is more than or equal to H/5 and less than or equal to K/4; when H/5 > K/2, D1 is equal to K/2.

Alternatively, the exit pupil diameter of the imaging objective 43 is H and the target surface size of the imaging unit 45 is K. Satisfies the following conditions: when H/2 is less than K, D2 is equal to K; when H/3 is greater than K, H/3 is greater than or equal to D2 is greater than or equal to H/2.

Optionally, D1 is more than or equal to 5.5mm and less than or equal to 7.5 mm. When imaging is performed in the central area 451, the diameter of the light passing hole 401 of the field stop 40 is greater than or equal to 5.5mm and less than or equal to 7.5 mm.

Optionally, 12mm ≦ D2 ≦ 14 mm. When the peripheral region 452 is imaged, the diameter of the light passing hole 401 of the field stop 40 is greater than or equal to 12mm and less than or equal to 14 mm.

Fig. 4 is a schematic structural diagram of another field stop provided in an embodiment of the present invention, and referring to fig. 4, the field stop 40 includes a stop plate 406 and a plurality of light passing holes 401 disposed on the stop plate 406. The plurality of light passing holes 401 include a first light passing hole 4071 and a second light passing hole 4072, and the diameter of the first light passing hole 4071 is smaller than the diameter of the second light passing hole 4072. In the embodiment of the present invention, when the central area 451 is used for imaging, the first light passing hole 4071 with a smaller diameter may be selected; when the peripheral region 452 is imaged, the second light passing hole 4072 with a larger diameter can be selected and used, and the size of the light passing hole 401 of the field stop 40 is changed to adapt to imaging of different regions, so that the central region and the peripheral region have the same brightness when being imaged, and the imaging quality is improved.

Illustratively, referring to FIG. 4, the diameter of the first light passing hole 4071 is D1, the diameter of the second light passing hole 4072 is D2, 5.5mm ≦ D1 ≦ 7.5mm, and 12mm ≦ D2 ≦ 14 mm.

Exemplarily, referring to fig. 4, the field stop 40 includes 2 first light passing holes 4071 and 2 second light passing holes 4072, the 2 first light passing holes 4071 are symmetrical with respect to the geometric center O of the stop plate 406, and the 2 second light passing holes 4072 are symmetrical with respect to the geometric center O of the stop plate 406. It should be noted that, in other embodiments, the field stop 40 may further include other numbers of light passing holes 401, and the embodiment of the present invention is not limited to the number of the light passing holes 401.

Alternatively, referring to fig. 1, the magnification of the imaging objective 43 is 10 times. When the imaging objective 43 with the power of 10 times is adopted to perform convergent imaging on light, a mode of fusing 25 photos can be used to form an image of a sample in a single hole of the complete hole plate. In other embodiments, the imaging objective 43 may also have other magnifications, such as 4 times.

Illustratively, the imaging objective 43 may be a finite distance objective, and the imaging objective 43 focuses the parallel light onto the imaging unit 45.

Fig. 5 is a schematic structural diagram of an illumination source provided by the embodiment of the present invention, referring to fig. 5, the illumination source 41 includes a lamp bead 2, a first lens 31, a second lens 32, a third lens 33, a bright field diaphragm 1, a fourth lens 34 and a fifth lens 35 which are arranged in sequence along an optical axis. First lens 31, second lens 32 and third lens 33 send light to lamp pearl 2 and assemble, so, set up bright field diaphragm 1 between third lens 33 and fourth lens 34, be favorable to carrying out selectivity printing opacity to the light beam after assembling through bright field diaphragm 1, improve imaging quality.

Optionally, with continued reference to fig. 1, the microscopic imaging system further comprises a tube lens 44, the tube lens 44 being located in the optical path between the field stop 40 and the imaging unit 45. The illumination light beam provided by the illumination light source 41 is projected onto the stage 42, converged by the imaging objective lens 43, filtered by the field stop 40, converged again by the tube lens 44, and imaged on the imaging unit 45.

For example, referring to fig. 1, the imaging objective 43 may be an infinity objective, and the light passing through the imaging objective 43 becomes parallel light, and the parallel light is filtered by the field stop 40 and then converged on the imaging unit 45 by the tube lens 44.

It should be noted that the foregoing is only a preferred embodiment of the present invention and the technical principles applied. It will be understood by those skilled in the art that the present invention is not limited to the particular embodiments described herein, but is capable of various obvious modifications, rearrangements, combinations and substitutions as will now become apparent to those skilled in the art without departing from the scope of the invention. Therefore, although the present invention has been described in greater detail with reference to the above embodiments, the present invention is not limited to the above embodiments, and may include other equivalent embodiments without departing from the scope of the present invention.

Claims

1. A microscopic imaging system is characterized by comprising an illumination light source, an imaging objective lens, an object stage and an imaging unit, wherein the illumination light source provides an illumination light beam, the object stage is used for bearing a pore plate, and after the illumination light beam is projected to a sample on the pore plate, the sample is imaged on the imaging unit by the imaging objective lens;

2. The microscopy imaging system of claim 1, wherein the sample is imaged at the imaging unit to include a central region and a peripheral region, the peripheral region surrounding the central region,

3. A microscopic imaging system according to claim 2, wherein the imaging objective has an exit pupil diameter H, and the imaging unit has a target surface size K;

when H/5 > K/2, D1 is equal to K/2.

4. A microscopic imaging system according to claim 2, wherein the imaging objective has an exit pupil diameter H, and the imaging unit has a target surface size K;

when H/2 is less than K, D2 is equal to K;

5. The microscopy imaging system of claim 2, wherein the central region is a cross.

6. The microscopic imaging system according to claim 1, wherein the diaphragm comprises a plectrum disc, a light-shielding scribe, a guide bar, and a driver;

7. The microscopic imaging system according to claim 1, wherein said field stop comprises a stop plate and a plurality of light passing apertures disposed on said stop plate;

8. The microscopic imaging system of claim 1, further comprising a tube mirror positioned on an optical path between the field stop and the imaging unit.

9. The microscopic imaging system according to claim 1, wherein the illumination source comprises a lamp bead, a first lens, a second lens, a third lens, a bright field stop, a fourth lens and a fifth lens arranged in sequence along an optical axis.

10. A microscopic imaging system according to claim 1, wherein said imaging objective has a magnification of 10.