CN214004675U - Simple device for fish cell in-vitro culture - Google Patents
Simple device for fish cell in-vitro culture Download PDFInfo
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- CN214004675U CN214004675U CN202022903233.6U CN202022903233U CN214004675U CN 214004675 U CN214004675 U CN 214004675U CN 202022903233 U CN202022903233 U CN 202022903233U CN 214004675 U CN214004675 U CN 214004675U
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Abstract
The utility model discloses a simple and easy device of fish cell in vitro culture, including temperature sensor, device body and welded plate, still including the liquid feeding mechanism that is used for making things convenient for temperature control's water-cooling mechanism, automatic nutrient feeding and the elevation structure that the cell of being convenient for was taken, the cold water dustcoat is installed in the outside of lid. The utility model discloses the work of heating piece produces the heat, make this internal environment heating of device, make this internal gas flow of device through the fan, make the temperature even, provide suitable temperature to the cell, detect the temperature through temperature sensor, when the high temperature, cool down through the water in the cold water dustcoat, through the work of electric hydraulic power micropump, water in the cold water dustcoat enters into heat exchanger interior heat transfer, the rethread refrigerator returns the cold water dustcoat after refrigerating fast in, cool down to the water in the cold water dustcoat, make this internal rapid cooling of device, the controlled temperature of being convenient for.
Description
Technical Field
The utility model relates to a cell culture device technical field specifically is a simple and easy device of fish cell external culture.
Background
With the development of biotechnology, more and more fish cell lines are established, and the specific methods for fish cell culture vary according to cell types, but generally, the fish cell culture technology is an important and potential biological research technical means and method, and the culture research of cells through a culture device, the survival rate of cells is determined by the temperature in an incubator, so that the proper temperature is very important.
With the continuous installation and use of simple devices for culturing fish cells in vitro in the market, the following problems are found in the using process:
1. the simple device for culturing the fish cells in vitro does not have a structure convenient for controlling the temperature in the incubator in the daily use process, and is not beneficial to improving the survival rate of the cells.
2. And the simple device for culturing the fish cells in vitro does not have a structure convenient for placing and taking the cells in the using process, thereby facilitating the work.
3. And the simple device for culturing the fish cells in vitro does not have a structure for automatically adding nutrient solution to the cells in the using process, so that the activity of the cells is enhanced.
Therefore, a simple device for culturing fish cells in vitro needs to be designed to solve the problems.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a simple and easy device of fish cell external culture to propose the current simple and easy device of fish cell external culture in solving above-mentioned background art and do not have the problem of controlled temperature, be convenient for take and add the nutritional supplement automatically.
In order to achieve the above object, the utility model provides a following technical scheme: a simple device for in vitro culture of fish cells comprises a temperature sensor, a device body, a welding plate, a water cooling mechanism for conveniently controlling the temperature, a liquid adding mechanism for automatically adding a nutrient and a lifting structure for conveniently taking cells;
the device comprises a device body and is characterized in that a cover body is installed at the top end of the device body, a cold water outer cover is installed on the outer side of the cover body, a gas storage tank is installed on the outer wall of one side of the cold water outer cover, a connecting pipe is connected to the top end of the gas storage tank, an installation pipe is connected to the lower portion of the connecting pipe, gas outlets are uniformly formed in the bottom end of the installation pipe, a temperature sensor is installed above the inner wall of one side of the device body, and a water cooling mechanism is installed on the outer wall of the other side of the cold water outer cover;
the device comprises a device body and is characterized in that a fixed pipe is arranged on the inner side below the device body, air inlets are uniformly formed in the top end of the fixed pipe, an incubator is arranged on the inner side of the device body, and a liquid adding mechanism is arranged on the outer wall of one side of the incubator;
the device is characterized in that a controller is installed on the outer wall of one end of the device body, a visible window is arranged above the controller, and the lifting structure is installed below the incubator.
Preferably, the water cooling mechanism comprises an electro-hydraulic power micropump, a cooling block, a refrigerator and a heat exchanger, the cooling block is installed on the outer wall of one side of the cold water outer cover, the inner walls of the two ends of the cooling block are provided with the electro-hydraulic power micropumps, the refrigerator is installed on the inner wall of one side of the cooling block, and the heat exchanger is installed on the inner wall of the other side of the cooling block.
Preferably, the fans are installed on two sides of the inner wall of the device body, and the heating blocks are evenly installed on the inner wall of the device body.
Preferably, the liquid feeding mechanism includes nutrient solution case, piston, the pneumatic telescopic link of second and feed liquor pipe, the nutrient solution case is installed in the outer wall of incubator, the pneumatic telescopic link of second is installed on the top of nutrient solution case, the top of the pneumatic telescopic link of second is connected with the piston, the bottom of nutrient solution case is connected with the feed liquor pipe.
Preferably, the lifting structure comprises a welding plate, the welding plate is mounted on the inner wall of the device body, a first pneumatic telescopic rod is mounted at the middle position of the top end of the welding plate, and one end of the first pneumatic telescopic rod is mounted at the bottom end of the incubator.
Preferably, the horizontal pole is all installed to the inner wall of welding plate both sides, and the outer wall of horizontal pole all installs articulated frame.
Preferably, extension springs are installed on one sides of the hinged frames, and one sides of the hinged frames are movably hinged to the outer wall of the first pneumatic telescopic rod.
Compared with the prior art, the beneficial effects of the utility model are that:
(1) the second pneumatic telescopic rod, the incubator, the liquid inlet pipe and the piston are arranged, the piston moves downwards to extrude the nutrient solution through the work of the second pneumatic telescopic rod, and then the nutrient solution enters the incubator through the liquid inlet pipe, so that the survival rate of cells is improved;
(2) the first pneumatic telescopic rod is arranged, the hinged frame and the incubator work, the first pneumatic telescopic rod extends to enable the incubator to drive cells to move upwards, the hinged frame achieves a supporting effect, and the hinged frame moves inwards at the same time, so that the incubator can be conveniently lifted, and the cells in the incubator can be conveniently taken;
(3) the utility model discloses a temperature control device for a refrigerator, including a heating block, an electro-hydraulic power micropump, a refrigerator, a temperature sensor, the cold water dustcoat, a heat exchanger, fan and device body, produce the heat through the work of heating block, make the heating of this internal environment of device, make this internal gas flow of device through the fan, make the temperature even, provide suitable temperature to the cell, detect the temperature through temperature sensor, when the high temperature, cool down through the water in the cold water dustcoat, through the work of electro-hydraulic power micropump, water in the cold water dustcoat enters into heat transfer in the heat exchanger, the rethread refrigerator cools off fast in the back returns the cold water dustcoat, cool down to the water in the cold water dustcoat, make this internal rapid cooling of device, the controlled temperature of being convenient for.
Drawings
FIG. 1 is a schematic cross-sectional view of the device of the present invention;
FIG. 2 is a schematic view of the front view structure of the device of the present invention;
FIG. 3 is a schematic view of the water cooling mechanism of the present invention;
FIG. 4 is a schematic structural view of the liquid adding mechanism of the present invention;
fig. 5 is an enlarged schematic view of a portion a of fig. 1 according to the present invention.
In the figure: 1. a fan; 2. a water cooling mechanism; 201. an electro-hydraulic power micropump; 202. cooling the block; 203. a refrigerator; 204. a heat exchanger; 3. a temperature sensor; 4. a cover body; 5. installing a pipe; 6. a connecting pipe; 7. an air outlet; 8. a gas storage tank; 9. a heating block; 10. a device body; 11. an air inlet; 12. a fixed tube; 13. a first pneumatic telescopic rod; 14. an incubator; 15. a cold water housing; 16. a visible window; 17. a controller; 18. a liquid adding mechanism; 1801. a nutrient solution tank; 1802. a piston; 1803. a second pneumatic telescopic rod; 1804. a liquid inlet pipe; 19. welding the plate; 20. a cross bar; 21. and a hinged frame.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Example 1: referring to fig. 1-5, a simple device for fish cell in vitro culture comprises a temperature sensor 3, a device body 10, a welding plate 19, a water cooling mechanism 2 for conveniently controlling temperature, a liquid adding mechanism 18 for automatically adding nutrient, and a lifting structure for conveniently taking cells;
a cover body 4 is installed at the top end of the device body 10, a cold water outer cover 15 is installed on the outer side of the cover body 4, a gas storage tank 8 is installed on the outer wall of one side of the cold water outer cover 15, a connecting pipe 6 is connected to the top end of the gas storage tank 8, an installation pipe 5 is connected to the lower portion of the connecting pipe 6, gas outlets 7 are evenly arranged at the bottom end of the installation pipe 5, a temperature sensor 3 is installed above the inner wall of one side of the device body 10, the type of the temperature sensor 3 can be WRM-101, and a water cooling mechanism 2 is installed on the outer wall of the other side of the cold water outer cover 15;
a fixed pipe 12 is arranged on the inner side below the device body 10, air inlets 11 are uniformly formed in the top end of the fixed pipe 12, an incubator 14 is arranged on the inner side of the device body 10, and a liquid adding mechanism 18 is arranged on the outer wall of one side of the incubator 14;
a controller 17 is arranged on the outer wall of one end of the device body 10, the model of the controller 17 can be JY-PH160, a visible window 16 is arranged above the controller 17, and the lifting structure is arranged below the incubator 14;
referring to fig. 1-5, the simple device for fish cell in vitro culture further comprises a lifting structure, the lifting structure comprises a welding plate 19, the welding plate 19 is mounted on the inner wall of the device body 10, a first pneumatic telescopic rod 13 is mounted in the middle of the top end of the welding plate 19, the type of the first pneumatic telescopic rod 13 can be HD20180111, and one end of the first pneumatic telescopic rod 13 is mounted at the bottom end of the incubator 14;
the inner walls of the two sides of the welding plate 19 are respectively provided with a cross rod 20, and the outer wall of each cross rod 20 is provided with a hinge frame 21;
one side of the hinged frame 21 is provided with a telescopic spring, and one side of the hinged frame 21 is movably hinged to the outer wall of the first pneumatic telescopic rod 13;
specifically, as shown in fig. 1 and 5, when the structure is used, the first pneumatic telescopic rod 13 works, the first pneumatic telescopic rod 13 extends to enable the incubator 14 to move upwards with the cells, the hinge frame 21 achieves a supporting effect, the hinge frame 21 moves inwards at the same time, and the incubator 14 is convenient to lift, so that the cells in the incubator 14 can be conveniently taken.
Example 2: the water cooling mechanism 2 comprises an electro-hydraulic power micropump 201, a cooling block 202, a refrigerator 203 and a heat exchanger 204, wherein the cooling block 202 is installed on the outer wall of one side of the cold water outer cover 15, the electro-hydraulic power micropumps 201 are installed on the inner walls of the two ends of the cooling block 202, the model of the electro-hydraulic power micropumps 201 can be SB-3-1, the refrigerator 203 is installed on the inner wall of one side of the cooling block 202, the model of the refrigerator 203 can be DTA-40, the heat exchanger 204 is installed on the inner wall of the other side of the cooling block 202, and the model of the heat exchanger 204 can be BIU 1100-4.35;
the two sides of the inner wall of the device body 10 are both provided with the fans 1, the model of the fan 1 can be YWF350-4, and the inner wall of the device body 10 is uniformly provided with the heating blocks 9;
specifically, as shown in fig. 1, fig. 2 and fig. 3, when using this structure, through the work heat production of heating block 9, make the interior environment heating of device body 10, make the interior gas flow of device body 10 through fan 1, make the temperature even, provide suitable temperature to the cell, detect the temperature through temperature sensor 3, when the temperature is too high, cool down through the water in the cold water dustcoat 15, through the work of electric hydraulic power micropump 201, the water in the cold water dustcoat 15 enters into heat exchanger 204 and exchanges heat, the rethread refrigerator 203 returns to in the cold water dustcoat 15 after refrigerating fast, cool down the water in the cold water dustcoat 15, make the interior rapid cooling of device body 10, adjust the temperature that suitable cell survived.
Example 3: the liquid adding mechanism 18 comprises a nutrient solution box 1801, a piston 1802, a second pneumatic telescopic rod 1803 and a liquid inlet pipe 1804, wherein the nutrient solution box 1801 is installed on the outer wall of the incubator 14, the top end of the nutrient solution box 1801 is provided with the second pneumatic telescopic rod 1803, the model of the second pneumatic telescopic rod 1803 can be YQL890, the top end of the second pneumatic telescopic rod 1803 is connected with the piston 1802, and the bottom end of the nutrient solution box 1801 is connected with the liquid inlet pipe 1804;
specifically, as shown in fig. 1 and 4, when the structure is used, the second pneumatic telescopic rod 1803 works to enable the piston 1802 to move downwards to extrude the nutrient solution, and then the nutrient solution enters the incubator 14 through the liquid inlet pipe 1804, so that the cell survival rate is improved.
The output end of the controller 17 is electrically connected with the input ends of the fan 1, the electro-hydraulic power micropump 201, the refrigerator 203, the heat exchanger 204, the heating block 9, the first pneumatic telescopic rod 13 and the second pneumatic telescopic rod 1803 through wires, and is electrically connected with the input end of the temperature sensor 3.
The working principle is as follows: when the device is used, firstly, by opening the air valve on the connecting pipe 6, carbon dioxide gas in the gas storage tank 8 enters the mounting pipe 5 through the connecting pipe 6, then enters the device body 10 through the air outlet 7 to provide required gas for cells and improve the survival rate of the cells, and the piston 1802 moves downwards to extrude nutrient solution by working through the second pneumatic telescopic rod 1803, and then the nutrient solution enters the culture box 14 through the liquid inlet pipe 1804 to improve the survival rate of the cells;
then, the heating block 9 works to generate heat, so that the environment in the device body 10 is heated, the air in the device body 10 flows through the fan 1, so that the temperature is uniform, a proper temperature is provided for cells, the temperature is detected through the temperature sensor 3, when the temperature is too high, the temperature is reduced through the water in the cold water housing 15, through the work of the electro-hydraulic power micropump 201, the water in the cold water housing 15 enters the heat exchanger 204 for heat exchange, and then returns to the cold water housing 15 after being rapidly cooled through the refrigerator 203, so that the water in the cold water housing 15 is cooled, so that the temperature in the device body 10 is rapidly reduced, and the temperature suitable for the survival of the cells is adjusted;
finally, the condition of cells can be observed through the visual window 16, the first pneumatic telescopic rod 13 works, the first pneumatic telescopic rod 13 extends to enable the incubator 14 to carry the cells to move upwards, the hinged frame 21 achieves a supporting effect, the hinged frame 21 moves inwards at the same time, and the lifting of the incubator 14 is facilitated, so that the cells in the incubator 14 can be taken conveniently.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. The utility model provides a simple and easy device of fish cell in vitro culture, includes temperature sensor (3), device body (10) and welds board (19), its characterized in that: the device also comprises a water cooling mechanism (2) for conveniently controlling the temperature, a liquid adding mechanism (18) for automatically adding a nutrient and a lifting structure for conveniently taking cells;
the device is characterized in that a cover body (4) is installed at the top end of the device body (10), a cold water outer cover (15) is installed on the outer side of the cover body (4), a gas storage tank (8) is installed on the outer wall of one side of the cold water outer cover (15), the top end of the gas storage tank (8) is connected with a connecting pipe (6), an installation pipe (5) is connected to the lower portion of the connecting pipe (6), gas outlets (7) are uniformly formed in the bottom end of the installation pipe (5), a temperature sensor (3) is installed above the inner wall of one side of the device body (10), and the water cooling mechanism (2) is installed on the outer wall of the other side of the cold water outer cover (15);
a fixed pipe (12) is arranged on the inner side below the device body (10), air inlets (11) are uniformly formed in the top end of the fixed pipe (12), an incubator (14) is arranged on the inner side of the device body (10), and a liquid adding mechanism (18) is arranged on the outer wall of one side of the incubator (14);
the device is characterized in that a controller (17) is installed on the outer wall of one end of the device body (10), a visible window (16) is arranged above the controller (17), and the lifting structure is installed below the incubator (14).
2. The simple device for fish cell in-vitro culture according to claim 1 is characterized in that: the water cooling mechanism (2) comprises an electro-hydraulic power micropump (201), a cooling block (202), a refrigerator (203) and a heat exchanger (204), wherein the cooling block (202) is installed on the outer wall of one side of a cold water outer cover (15), the inner walls of the two ends of the cooling block (202) are provided with the electro-hydraulic power micropump (201), the refrigerator (203) is installed on the inner wall of one side of the cooling block (202), and the heat exchanger (204) is installed on the inner wall of the other side of the cooling block (202).
3. The simple device for fish cell in-vitro culture according to claim 1 is characterized in that: fan (1) are all installed to the both sides of device body (10) inner wall, heating piece (9) are evenly installed to the inner wall of device body (10).
4. The simple device for fish cell in-vitro culture according to claim 1 is characterized in that: liquid feeding mechanism (18) include nutrient solution case (1801), piston (1802), second pneumatic telescopic link (1803) and feed liquor pipe (1804), the outer wall in incubator (14) is installed in nutrient solution case (1801), second pneumatic telescopic link (1803) are installed to the top of nutrient solution case (1801), the top of second pneumatic telescopic link (1803) is connected with piston (1802), the bottom of nutrient solution case (1801) is connected with feed liquor pipe (1804).
5. The simple device for fish cell in-vitro culture according to claim 1 is characterized in that: the lifting structure comprises a welding plate (19), wherein the welding plate (19) is installed on the inner wall of the device body (10), a first pneumatic telescopic rod (13) is installed at the middle position of the top end of the welding plate (19), and one end of the first pneumatic telescopic rod (13) is installed at the bottom end of the incubator (14).
6. The simple device for fish cell in-vitro culture according to claim 5 is characterized in that: horizontal pole (20) are all installed to the inner wall of welding plate (19) both sides, and articulated frame (21) are all installed to the outer wall of horizontal pole (20).
7. The simple device for fish cell in-vitro culture according to claim 6, is characterized in that: extension springs are installed on one sides of the hinged frames (21), and one sides of the hinged frames (21) are movably hinged to the outer wall of the first pneumatic telescopic rod (13).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022903233.6U CN214004675U (en) | 2020-12-04 | 2020-12-04 | Simple device for fish cell in-vitro culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022903233.6U CN214004675U (en) | 2020-12-04 | 2020-12-04 | Simple device for fish cell in-vitro culture |
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| Publication Number | Publication Date |
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| CN214004675U true CN214004675U (en) | 2021-08-20 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202022903233.6U Active CN214004675U (en) | 2020-12-04 | 2020-12-04 | Simple device for fish cell in-vitro culture |
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| CN (1) | CN214004675U (en) |
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