CN213977665U - Cell culture liquid subpackaging device - Google Patents
Cell culture liquid subpackaging device Download PDFInfo
- Publication number
- CN213977665U CN213977665U CN202022875017.5U CN202022875017U CN213977665U CN 213977665 U CN213977665 U CN 213977665U CN 202022875017 U CN202022875017 U CN 202022875017U CN 213977665 U CN213977665 U CN 213977665U
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- Prior art keywords
- rotating shaft
- box
- bevel gear
- cell culture
- bottom plate
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- 239000007788 liquid Substances 0.000 title claims abstract description 47
- 238000004113 cell culture Methods 0.000 title claims abstract description 39
- 238000010438 heat treatment Methods 0.000 claims abstract description 63
- 238000004140 cleaning Methods 0.000 claims description 16
- 238000003756 stirring Methods 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 5
- 229920000742 Cotton Polymers 0.000 claims description 4
- 241000219146 Gossypium Species 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 239000000428 dust Substances 0.000 description 6
- 241000220317 Rosa Species 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
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Abstract
The utility model discloses a partial shipment device of cell culture liquid, include: the bottom plate, the bottom plate bottom is equipped with the suction mechanism, the suction mechanism includes the motor, motor fixed mounting is in the bottom plate bottom, motor output shaft fixedly connected with pivot one, the fixed bevel gear one that is equipped with in a pivot outside, bevel gear one side is equipped with bevel gear two, bevel gear two meshes with bevel gear one mutually, bevel gear two one side fixedly connected with pivot two, bottom plate bottom fixedly connected with bellows, a partial shipment device of cell culture liquid beneficial effect is: the puddler rotates and to make the air in the short time even heating, the condition of local heat can not appear and reduce the air heating time, can guarantee the clean cleanness of air, and during the hot-air got into flourishing liquid bottle through pipe one, cell culture liquid got into the scale bottle through pipe two, can the ration partial shipment cell culture liquid through the control switch valve.
Description
Technical Field
The utility model relates to a cell culture technical field especially relates to a partial shipment device of cell culture liquid.
Background
At present, the methods for supplementing culture solution in cell culture are simple, and generally a pipette or a liquid aspirator is used for sucking the required culture solution into a cell culture bottle for many times. In the prior art, a pipette or a pipette is generally used for sucking the nutrient solution in an aseptic storage bottle and then transferring the nutrient solution to various culture bottles. However, this method requires repeated operations to suck the culture solution into the target cell culture flask, which is time-consuming and labor-consuming. Moreover, the operation is not in a closed system, and the cell contamination is easily caused by improper operation.
The prior art has the following defects: all be manual operation when carrying out cell culture liquid partial shipment at present, aseptic chamber number of times of opening a door and non-operating personnel get into can be more frequent when the manual work carries out the partial shipment, the air of aseptic chamber will circulate with outside air for a long time like this, probably cause culture solution secondary pollution, can influence the quality of cell culture liquid, manual partial shipment cell culture liquid speed is slow, the inefficiency, some culture liquids need many people to cooperate when the partial shipment just can accomplish, intensity of labour is higher, and quantitative standard can descend when many people cooperate the partial shipment, can not accomplish quantitative partial shipment.
Therefore, in view of the above technical problems, it is necessary for those skilled in the art to develop a dispensing device for cell culture solution.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a partial shipment device of cell culture liquid.
In order to realize the purpose of partial shipment, the utility model provides a following technical scheme:
the utility model discloses a partial shipment device of cell culture liquid, include: the bottom plate, the bottom plate bottom is equipped with the suction mechanism, the bottom plate top is equipped with heating mechanism.
The air suction mechanism comprises a motor, the motor is fixedly arranged at the bottom of the bottom plate, a first rotating shaft is fixedly connected with an output shaft of the motor, a first bevel gear is fixedly arranged on the outer side of the first rotating shaft, a second bevel gear is arranged on one side of the first bevel gear and meshed with the first bevel gear, a second rotating shaft is fixedly connected with one side of the second bevel gear, an air box is fixedly connected with the bottom of the bottom plate, two filter screens are fixedly embedded in the air box, one end of the second rotating shaft extends into the air box, an air suction part is fixedly arranged on the outer part of the second rotating shaft and positioned on the inner sides of the two filter screens, and two cleaning brushes are fixedly arranged on the outer side of the second rotating shaft;
the heating mechanism comprises a heating box, the heating box is fixedly arranged at the top of a bottom plate, a first connecting pipe is fixedly connected between the bottom of the heating box and the top of an air box, the top end of a first rotating shaft extends to the top of the heating box, a plurality of heating sheets are fixedly arranged on the inner walls of the two sides of the heating box and are symmetrically distributed on the two sides of the inner wall of the heating box, a fan blade and a plurality of stirring rods are fixedly arranged at the outer side of the first rotating shaft, the fan blade is positioned at the tops of the stirring rods, a filter box is fixedly connected at the top of the heating box, two second connecting pipes are fixedly connected at the bottom of the filter box and the top of the heating box, two filter cottons are fixedly arranged in the filter box, a scale bottle and a liquid containing bottle are fixedly arranged at the top of the bottom plate, the liquid containing bottle is positioned at the inner sides of the air box and the scale bottle, a first guide pipe is fixedly connected at the top of the filter box, and one end of the guide pipe extends to the inside of the liquid containing bottle, the top of the liquid containing bottle is fixedly connected with a second conduit, one end of the second conduit extends into the liquid containing bottle, and the other end of the second conduit extends into the scale bottle.
Further, the first rotating shaft is movably connected with the joint of the bottom plate and the heating box through a bearing, the second rotating shaft is movably connected with the joint of the air box through a bearing, and the second rotating shaft is movably connected with the joint of the filter screen through a bearing sealing ring.
Further, two cleaning brushes are respectively contacted with two filter screens.
Furthermore, a switch valve is arranged on the outer side of the second guide pipe, and bases are fixedly connected to the two sides of the bottom plate.
Furthermore, the air suction component comprises a fan, and the fan is fixedly sleeved on the outer side of the second rotating shaft.
Furthermore, the air suction component comprises two fans which are fixedly sleeved on the outer sides of the second rotating shaft.
In the technical scheme, the utility model provides a pair of partial shipment device of cell culture liquid has following beneficial effect:
1. the utility model discloses a motor rotates and drives pivot one and rotate, and pivot one drives bevel gear and rotates, and bevel gear drives bevel gear two and rotates, and bevel gear two drives pivot two and rotates, and pivot two drive the fan in the outside and rotate, and the circulation of air has been accelerated in the fan rotation, and bellows inside inlays and is equipped with the filter screen, and the filter screen can filter the dust in the air, and the pivot two outsides still is equipped with cleaning brush, and cleaning brush contacts with the filter screen, and cleaning brush can clean the dust that the filter screen surface is piled up, can not cause the filter screen to block up;
2. the utility model discloses a motor drives pivot one and rotates, a pivot top extends to the heating cabinet top, heating cabinet both sides inner wall all is equipped with a plurality of heating plates, can let the air heating that flows into in the heating cabinet and detach the bacterium, a pivot outside still is equipped with puddler and fan, the puddler rotates and to make the air in the short time even heating, local hot condition can not appear and reduce the air heating time, the fan rotates the air that can make after the heating and flows into the rose box, the air can carry out secondary filter in the rose box, can guarantee the clean cleanness of air, during hot-air gets into the flourishing liquid bottle through pipe one, cell culture liquid passes through pipe two and gets into the scale bottle, can be with quantitative partial shipment cell culture liquid through the control switch valve.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present invention, and other drawings can be obtained by those skilled in the art according to these drawings.
Fig. 1 is a schematic structural diagram of a cell culture solution split charging device provided in an embodiment of the present invention;
FIG. 2 is an enlarged view of part A of a schematic structural diagram of a separate-loading device for cell culture solution according to an embodiment of the present invention;
FIG. 3 is an enlarged view of part B of a schematic structural diagram of a separate-loading device for cell culture solution according to an embodiment of the present invention;
fig. 4 is a schematic structural diagram of embodiment 2 of a cell culture solution dispensing device according to an embodiment of the present invention.
Description of reference numerals:
the device comprises a base plate 1, a motor 2, a first rotating shaft 3, a first bevel gear 4, a second bevel gear 5, a second rotating shaft 6, a filter screen 7, a fan 8, a cleaning brush 9, a first connecting pipe 10, a heating plate 11, a stirring rod 12, fan blades 13, a second connecting pipe 14, filter cotton 15, a scale bottle 16, a liquid containing bottle 17, a first conduit 18, a second conduit 19, a switch valve 20 and a base 21.
Detailed Description
In order to make the technical solution of the present invention better understood by those skilled in the art, the present invention will be further described in detail with reference to the accompanying drawings.
Example 1:
see fig. 1-3;
the utility model discloses a partial shipment device of cell culture liquid, include: the bottom plate 1, 1 bottom of bottom plate is equipped with the suction mechanism, 1 top of bottom plate is equipped with heating mechanism.
The air suction mechanism comprises a motor 2, the motor 2 is fixedly arranged at the bottom of a bottom plate 1, an output shaft of the motor 2 is fixedly connected with a first rotating shaft 3, a first bevel gear 4 is fixedly arranged on the outer side of the first rotating shaft 3, a second bevel gear 5 is arranged on one side of the first bevel gear 4, the second bevel gear 5 is meshed with the first bevel gear 4, a second rotating shaft 6 is fixedly connected on one side of the second bevel gear 5, an air box is fixedly connected with the bottom of the bottom plate 1, two filter screens 7 are fixedly embedded in the air box, one end of the second rotating shaft 6 extends into the air box, an air suction part is fixedly arranged on the outer portion of the second rotating shaft 6, the air suction part is positioned on the inner sides of the two filter screens 7, and two cleaning brushes 9 are fixedly arranged on the outer side of the second rotating shaft 6;
the heating mechanism comprises a heating box, the heating box is fixedly arranged at the top of a bottom plate 1, a first connecting pipe 10 is fixedly connected between the bottom of the heating box and the top of an air box, the top end of a first rotating shaft 3 extends to the top of the heating box, a plurality of heating sheets 11 are fixedly arranged on the inner walls of the two sides of the heating box, the plurality of heating sheets 11 are symmetrically distributed on the two sides of the inner wall of the heating box, a fan blade 13 and a plurality of stirring rods 12 are fixedly arranged on the outer side of the first rotating shaft 3, the fan blade 13 is positioned at the top of the plurality of stirring rods 12, a filtering box is fixedly connected with the top of the heating box, two second connecting pipes 14 are fixedly connected between the bottom of the filtering box and the top of the heating box, two filter cottons 15 are fixedly arranged in the filtering box, a scale bottle 16 and a liquid containing bottle 17 are fixedly arranged on the top of the bottom plate 1, the liquid containing bottle 17 is positioned on the inner side of the air box and the scale bottle 16, a first guiding pipe 18 is fixedly connected with the top of the filtering box, one end of the first conduit 18 extends into the liquid containing bottle 17, the top of the liquid containing bottle 17 is fixedly connected with a second conduit 19, one end of the second conduit 19 extends into the liquid containing bottle 17, and the other end of the second conduit 19 extends into the scale bottle 16.
Preferably, pivot one 3 all passes through bearing swing joint with bottom plate 1 and heating cabinet junction, two 6 bearing swing joints with the bellows junction of pivot, two 6 bearing sealing washer swing joint are passed through with the 7 junctions of filter screen in the pivot, and the multiple places can increase life through swing joint, can the long-time continuation work.
Preferably, two cleaning brushes 9 are respectively contacted with two filter screens 7, and the cleaning brushes 9 are contacted with the filter screens 7 to clean dust on the surfaces of the filter screens 7, so that the accumulation condition is avoided.
Preferably, the outer side of the second conduit 19 is provided with a switch valve 20, the two sides of the bottom plate 1 are fixedly connected with a base 21, and the switch valve 20 can control the flow of the cell culture solution and can perform quantitative sub-packaging.
Preferably, the air suction component comprises a fan 8, the fan 8 is fixedly sleeved outside the second rotating shaft 6, and the flow rate of air can be increased by rotating the fan 8.
The implementation mode is specifically as follows: the motor 2 rotates to drive the first rotating shaft 3 to rotate, the first rotating shaft 3 rotates to drive the first bevel gear 4 to rotate, the first bevel gear 4 drives the second bevel gear 5 to rotate, the second bevel gear 5 drives the second rotating shaft 6 to rotate, the second rotating shaft 6 rotates to drive the fan 8 to rotate, the fan 8 rotates to accelerate air flow rate, the second rotating shaft 6 rotates to drive the cleaning brush 9 to rotate, the cleaning brush 9 rotates to clean dust on the surface of the filter screen 7, a heating plate 11 is arranged inside the heating box, the heating plate 11 heats air and removes bacteria, the first rotating shaft 3 rotates to drive the stirring rod 12 to rotate, the stirring rod 12 rotates to enable air to be heated more uniformly, air heating time is reduced, the first rotating shaft 3 rotates to drive the fan blades 13 to rotate, the fan blades 13 rotate to heat air to enter the filter box, hot air flows into the liquid containing bottle 17 through the first guide pipe 18, cell culture liquid in the liquid containing bottle 17 enters the scale bottle 16 through the second guide pipe 19, and the control switch valve 20 can quantitatively contain the cell culture liquid.
Example 2:
the difference from example 1 is:
as shown in fig. 4;
the utility model discloses a partial shipment device of cell culture liquid, the part of breathing in includes two fans 8, two fan 8 is all fixed cup joints in two 6 outsides in the pivot.
The implementation mode is specifically as follows: two fans 8 suction of fixed cup joint in the 6 outside of pivot two are stronger, the slow condition of air flow rate can not appear, can have more air admission heating cabinet, solved the untimely condition of hot-air supply, let more hot-air pass through pipe 18 and get into flourishing liquid bottle 17 in, fan 8 rotates the in-process and inevitably appears damaging, this problem has been solved to two fans 8 perfect, one of them fan 8 damages and has another fan 8 can work in addition, can not influence the partial shipment work of cell culture liquid.
In the technical scheme, the utility model provides a pair of partial shipment device of cell culture liquid has following beneficial effect:
1. the utility model discloses a motor 2 rotates and drives pivot one 3 and rotate, pivot one 3 drives bevel gear one 4 and rotates, bevel gear one 4 drives bevel gear two 5 and rotates, bevel gear two 5 drives pivot two 6 and rotates, pivot two 6 drives the fan 8 rotation in the outside, the circulation of air has been accelerated in the fan 8 rotation, and bellows inside inlays and is equipped with filter screen 7, filter screen 7 can filter the dust in the air, and the pivot two 6 outside still is equipped with cleaning brush 9, cleaning brush 9 contacts with filter screen 7, cleaning brush 9 can clean the dust that accumulates on filter screen 7 surface, can not cause filter screen 7 to block up;
2. the utility model discloses a motor 2 drives pivot 3 and rotates, 3 tops of pivot extend to the heating cabinet top, heating cabinet both sides inner wall all is equipped with a plurality of heating plates 11, can let the air heating that flows into in the heating cabinet and detach the bacterium, 3 outsides in the pivot still are equipped with puddler 12 and fan 8, puddler 12 rotates and to make the air even heating in the short time, the condition of local heat can not appear and reduce the air heating time, fan 8 rotates and to make the air after the heating flow into the rose box, the air can carry out secondary filter in the rose box, can guarantee the clean cleanness of air, hot-air passes through pipe 18 and gets into in flourishing liquid bottle 17, cell culture liquid passes through pipe two 19 and gets into scale bottle 16, can divide partial shipment cell culture liquid with ration through control switch valve 20.
While certain exemplary embodiments of the present invention have been described above by way of illustration only, it will be apparent to those of ordinary skill in the art that the described embodiments may be modified in various different ways without departing from the spirit and scope of the present invention. Accordingly, the drawings and description are illustrative in nature and should not be construed as limiting the scope of the invention.
Claims (6)
1. A split charging device for cell culture solution is characterized by comprising: the heating device comprises a bottom plate (1), wherein a suction mechanism is arranged at the bottom of the bottom plate (1), and a heating mechanism is arranged at the top of the bottom plate (1);
the air suction mechanism comprises a motor (2), the motor (2) is fixedly arranged at the bottom of the bottom plate (1), an output shaft of the motor (2) is fixedly connected with a first rotating shaft (3), a first bevel gear (4) is fixedly arranged on the outer side of the first rotating shaft (3), a second bevel gear (5) is arranged on one side of the first bevel gear (4), the second bevel gear (5) is meshed with the first bevel gear (4), one side of the second bevel gear (5) is fixedly connected with a second rotating shaft (6), the bottom of the bottom plate (1) is fixedly connected with an air box, two filter screens (7) are fixedly embedded in the air box, one end of the second rotating shaft (6) extends into the air box, an air suction component is fixedly arranged outside the second rotating shaft (6), the air suction component is positioned on the inner sides of the two filter screens (7), two cleaning brushes (9) are fixedly arranged on the outer side of the second rotating shaft (6);
the heating mechanism comprises a heating box, the heating box is fixedly arranged at the top of a bottom plate (1), a first connecting pipe (10) is fixedly connected with the bottom of the heating box and the top of an air box, the top end of a first rotating shaft (3) extends to the top of the heating box, a plurality of heating sheets (11) are fixedly arranged on the inner walls of the two sides of the heating box, the heating sheets (11) are symmetrically distributed on the two sides of the inner wall of the heating box, a fan blade (13) and a plurality of stirring rods (12) are fixedly arranged on the outer side of the first rotating shaft (3), the fan blade (13) is arranged at the tops of the stirring rods (12), a filter box is fixedly connected with the top of the heating box, two second connecting pipes (14) are fixedly connected with the bottom of the filter box and the top of the heating box, two filter cottons (15) are fixedly arranged inside the filter box, a scale bottle (16) and a liquid containing bottle (17) are fixedly arranged at the top of the bottom plate (1), the liquid containing bottle (17) is located on the inner side of the air box and the scale bottle (16), a first guide pipe (18) is fixedly connected to the top of the filtering box, one end of the first guide pipe (18) extends to the inside of the liquid containing bottle (17), a second guide pipe (19) is fixedly connected to the top of the liquid containing bottle (17), one end of the second guide pipe (19) extends to the inside of the liquid containing bottle (17), and the other end of the second guide pipe (19) extends to the inside of the scale bottle (16).
2. The split charging device for the cell culture solution according to claim 1, wherein the joints of the first rotating shaft (3) and the bottom plate (1) and the heating box are movably connected through bearings, the joint of the second rotating shaft (6) and the air box is movably connected through bearings, and the joint of the second rotating shaft (6) and the filter screen (7) is movably connected through a bearing sealing ring.
3. A dispensing device for cell culture solution according to claim 1, characterized in that two cleaning brushes (9) are respectively in contact with two sieves (7).
4. The split charging device for the cell culture solution according to claim 1, wherein a switch valve (20) is arranged outside the second conduit (19), and bases (21) are fixedly connected to two sides of the bottom plate (1).
5. The split charging device for the cell culture solution according to claim 1, wherein the air suction component comprises a fan (8), and the fan (8) is fixedly sleeved outside the second rotating shaft (6).
6. The split charging device for the cell culture solution according to claim 1, wherein the air suction component comprises two fans (8), and the two fans (8) are fixedly sleeved outside the second rotating shaft (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022875017.5U CN213977665U (en) | 2020-12-02 | 2020-12-02 | Cell culture liquid subpackaging device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022875017.5U CN213977665U (en) | 2020-12-02 | 2020-12-02 | Cell culture liquid subpackaging device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213977665U true CN213977665U (en) | 2021-08-17 |
Family
ID=77239593
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022875017.5U Expired - Fee Related CN213977665U (en) | 2020-12-02 | 2020-12-02 | Cell culture liquid subpackaging device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213977665U (en) |
-
2020
- 2020-12-02 CN CN202022875017.5U patent/CN213977665U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210817 |