CN213951145U

CN213951145U - Cell recovery water bath

Info

Publication number: CN213951145U
Application number: CN202022667024.6U
Authority: CN
Inventors: 王大鹏; 王秀娥; 张雅楠; 武嘉钊; 牟春琳; 张昕艳; 李政楠
Original assignee: Huayu Biotechnology Tianjin Co ltd
Current assignee: Huayu Biotechnology Tianjin Co ltd
Priority date: 2020-11-18
Filing date: 2020-11-18
Publication date: 2021-08-13
Anticipated expiration: 2030-11-18

Abstract

The utility model relates to the technical field of cell resuscitation devices, in particular to a cell resuscitation water bath, which comprises a bath body, wherein a platform driven by a motor is arranged in the bath body; the output end of the motor is connected with the linear reciprocating mechanism, and the linear reciprocating mechanism is connected with the platform; the platform is provided with a support body, and the support body is used for placing and fixing the cell resuscitation plate; the cell recovery plate comprises a plate body, at least one strip-shaped crack is arranged on the plate body, and the width of the strip-shaped crack is between the diameter of the tube body of the cell cryopreservation tube and the diameter of the tube cover; at least one tail end of the long-strip-shaped crack is set to be an expansion end, and the diameter of the expansion end is not smaller than the diameter of a tube cover of the cell cryopreservation tube; the expansion end is provided with a cover body for covering the expansion end. The utility model provides a cell resuscitation water bath has solved among the prior art cryopreserving pipe resuscitation in-process and has easily taken off the hand, easily pollute, be heated inhomogeneous, the inefficiency scheduling problem.

Description

Cell recovery water bath

Technical Field

The utility model relates to a cell recovery technical field especially relates to a cell recovery water bath.

Background

The cryopreservation tube is a commonly used cell sample cryopreservation device, and is generally applied to biological and medical experiments. In the process of recovering the cell sample, the cryopreservation tube needs to be placed in a water bath kettle, the cryopreservation tube is shaken to enable cells in the cryopreservation tube to be uniformly heated, and the cryopreservation tube is easy to fall off in the shaking and unfreezing process. In addition, the biological sample in the cryopreservation tube is not beneficial to being uniformly heated. And if a plurality of cells are revived at one time, a plurality of experimenters are needed to operate together, great inconvenience is brought to the experimenters, and the efficiency is very low.

SUMMERY OF THE UTILITY MODEL

The present invention aims at least solving one of the technical problems existing in the prior art or the related art.

In order to achieve the aim, the utility model provides a cell resuscitation water bath which comprises a bath body, wherein a platform driven by a motor is arranged in the bath body;

the output end of the motor is connected with a linear reciprocating mechanism, and the linear reciprocating mechanism is connected with the platform;

the platform is provided with a support body, and the support body is used for placing and fixing a cell resuscitation plate;

the cell recovery plate comprises a plate body, at least one strip-shaped crack is arranged on the plate body, and the width of the strip-shaped crack is between the diameter of the tube body of the cell cryopreservation tube and the diameter of the tube cover;

at least one tail end of the long-strip-shaped crack is set to be an expansion end, and the diameter of the expansion end is not smaller than the diameter of a tube cover of the cell cryopreservation tube;

the expansion end is provided with a cover body used for covering the expansion end.

In some possible embodiments, the support is at least one upright structure;

the upright post structure is provided with a movable barrier, the cell recovery plate is provided with a through hole matched with the upright post structure, and the through hole of the cell recovery plate penetrates through the upright post structure and stays to the barrier.

In some possible embodiments, the stud structure is provided with a thread along its length, and the obstacle is provided with a thread adapted to the thread of the stud structure, the obstacle being in threaded connection with the stud structure.

In some possible embodiments, the support body is a pillar structure, and the middle position of the cell resuscitation plate is provided with a through hole matched with the pillar structure;

and the through hole of the cell recovery plate penetrates through the upright post structure and stays at the barrier, and then the cell recovery plate is fixed by a fixture.

In some possible embodiments, the motor is further connected with a controller for controlling the rotation speed of the motor, and the operation panel of the cell resuscitation water bath is correspondingly provided with a regulating component for controlling the rotation speed of the motor.

In some possible embodiments, a water level sensor and a temperature sensor are arranged in the tank body, and a temperature display screen and a water level display screen are correspondingly arranged on an operation panel of the cell resuscitation water bath tank.

In some possible embodiments, a heater is arranged in the tank body, and an operation panel of the cell resuscitation water bath is provided with a temperature regulator and a temperature comparator;

the temperature comparator is respectively connected with the temperature sensor and the temperature regulator, and the temperature comparator is used for calculating the difference value between the temperature value of the temperature sensor and the temperature value of the temperature regulator and determining to turn on or turn off the heater.

In some possible embodiments, at least two elongated slits are provided on the plate body, the width of the elongated slits is at least one different, and the elongated slits are arranged in parallel.

In some possible embodiments, one end of the elongated slit is provided as an enlarged end, and the same end of the elongated slit is provided with an enlarged end.

In some possible embodiments, the slit of the enlarged end is provided in a circular shape.

Compared with the prior art, the utility model following beneficial effect has:

(1) the utility model provides a cell resuscitation water bath has solved among the prior art cryopreserving pipe resuscitation in-process and has easily taken off the hand, easily pollute, be heated inhomogeneous, the inefficiency scheduling problem.

(2) The utility model provides a cell recovery water bath, the long banding crack of the cell recovery board wherein through setting up different width has solved the problem of resuscitating when different diameter cells cryopreserved pipe.

(3) The utility model provides a cell resuscitation water bath still is including being used for covering the lid of inflation end freezes to deposit the pipe at the cell and puts into the back, through covering the inflation end, prevents that the cell from freezing to deposit the pipe and sliding into the inflation end and drop.

(4) The utility model provides a cell recovery water bath, the reciprocating motion through the supporter drives the cell recovery board motion on it, reaches the purpose that external mechanical force freezes the cell in the pipe with cell cryopreservation, practices thrift the manpower.

Drawings

The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:

fig. 1 is a schematic structural diagram of an external shape of a cell resuscitation water bath provided by an embodiment of the present invention;

fig. 2 is a schematic structural diagram of a cell resuscitation plate according to an embodiment of the present invention;

fig. 3 is a schematic structural diagram of another cell resuscitation plate according to an embodiment of the present invention;

fig. 4 is a schematic structural diagram of another cell resuscitation plate according to an embodiment of the present invention;

fig. 5 is a schematic structural diagram of another cell resuscitation plate according to an embodiment of the present invention;

fig. 6 is a schematic structural diagram of another cell resuscitation plate according to an embodiment of the present invention;

wherein, the correspondence between the reference numbers and the part names in fig. 1 to 6 is:

100-groove body; 110-cell resuscitation plate; 111-elongated slits; 112-an enlarged end; 113-a via; 114-a lid; 200-an operation panel; 300-water pipe.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention more clearly understood, the present invention will be described in further detail with reference to the accompanying drawings and detailed description. It should be noted that the embodiments and features of the embodiments of the present application may be combined with each other without conflict.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, however, the present invention may be practiced in other ways than those specifically described herein, and therefore the scope of the present invention is not limited to the specific embodiments disclosed below.

A cell resuscitation water bath according to the present invention is described below with reference to fig. 1 to 6.

The embodiment of the utility model provides a cell recovery water bath, as shown in fig. 1-6, comprises a bath body 100, wherein a platform driven by a motor is arranged in the bath body 100;

the platform is provided with a support for placing and fixing a cell resuscitation plate 110;

the cell recovery plate 110 comprises a plate body, at least one strip-shaped crack 111 is arranged on the plate body, and the width of the strip-shaped crack 111 is between the diameter of the tube body of the cell cryopreservation tube and the diameter of the tube cover;

at least one tail end of the long-strip-shaped crack 111 is set to be an expanded end 112, and the diameter of the expanded end 112 is not smaller than that of a tube cover of the cell cryopreservation tube;

the enlarged end 112 is provided with a cover 114 for covering the enlarged end 112.

In the current experiment operation, the cell freezing tube is held by hand and shaken in the tank body 100 of the water bath kettle to achieve the purpose of thawing the cells therein. On one hand, the number of the cell freezing tubes which can be held by hands is limited, and the cells are easy to slide off when the cells are shaken; on the other hand, the manual shaking is labor-consuming and is not easy to control the time.

Based on this, the utility model provides a cell resuscitation water bath, when using, put the cell cryopreservation pipe into the cell resuscitation plate 110, the lid 114 covers the expansion end 112; then the cell recovery plate 110 is put on the support body, and the motor is started to make the cell recovery plate 110 reciprocate on the support body at a proper speed, so as to achieve the purpose of continuously thawing the cells in the cell freezing tube.

Because the diameter difference between the tube body and the tube cover of the cell cryopreservation tube is small, the long-strip-shaped crack 111 is arranged on the plate body and is directly placed into the cell cryopreservation tube, and the cell cryopreservation tube is difficult to place due to different placing angles; in addition, even after the cell cryopreservation tube is placed in the long-strip-shaped crack 111, the crack is gradually increased due to the acting force in the placing process, and the cell cryopreservation tube slowly falls from the long-strip-shaped crack 111 easily, so that the service life of the plate body is shortened.

The utility model discloses in, the cell cryopreserved pipe is put into from cell resuscitation board 110's expansion end 112, then freezes the tube cap of depositing the pipe with the cell and is located the plate body top, and the body that is close to the cell cryopreserved pipe tube cap removes through the rectangular form crack 111 of expansion end 112 for a plurality of cells cryopreserved pipe arrange in proper order in rectangular form crack 111, and last lid 114 covers expansion end 112. The cell cryopreservation tube is placed in a sliding mode, so that the operability of placing the cell cryopreservation tube into the long-strip-shaped crack 111 is improved, the acting force on the width of the long-strip-shaped crack 111 in the sliding process is small, and the width of the long-strip-shaped crack 111 is basically not obviously influenced, so that the service life of the resuscitation plate is effectively prolonged.

The diameters of the tube bodies of the commonly used cell freezing tubes are 1cm, 1.25cm, 1.5cm, 2cm, 3cm and the like. The diameter of the tube cover of the cell freezing tube is slightly larger than that of the tube body, and is generally 0.2-0.5cm larger than that of the tube body. The utility model discloses in, rectangular form crack 111's width is between the diameter of tube cap and the diameter of body. Generally, the width of the elongated slit 111 is set closer to the diameter of the tube body of the cell cryopreservation tube than the diameter of the tube cover of the cell cryopreservation tube, which is mainly considered to be able to more firmly fix the cell cryopreservation tube in the elongated slit 111.

In addition, the main body part of the tube body of the freezing and storing tube is generally a cylinder with the same diameter at the upper part and the lower part, the tube bottom of the freezing and storing tube is in a circular arc shape or a cone shape, and the tube bottom of the freezing and storing tube is also provided with an additional hollow cylinder or other parts consisting of supporting pieces on the basis of the circular arc shape or the cone shape. In addition, the upper and lower different diameters of the tube body of some freezing tubes are generally that the lower part is narrow and gradually thicker. For the cylindrical tube body with the same diameter at the upper part and the lower part, the width of the long-strip-shaped crack 111 of the utility model is between the diameter of the tube body of the cell cryopreservation tube and the diameter of the tube cover; to the body of the cylinder of different diameters from top to bottom, the width of long form crack 111 of the utility model is generally between the diameter of the body that is close to the tube cap part and the tube cap diameter.

The utility model discloses in, body or the tube cap of cell cryopreserving pipe are not only limited to the cylinder, still can be for other shapes, if be other shapes, then the straight line numerical value between the minimum opposite face is chooseed for use to the diameter of body, and the straight line numerical value between the minimum opposite face is also chooseed for use to the diameter of tube cap. If the body of cell cryopreserving pipe is the cuboid, the tube cap of cell cryopreserving pipe also is the cuboid, then the diameter of body is the minor face numerical value of cuboid side, and the minor face numerical value of cuboid side is also chooseed for use to the diameter of tube cap. Others are not listed.

After the cell cryopreservation tube slides from the expansion end 112 to the long-strip-shaped crack 111, if the angle for taking the cell to recover the water bath is different, if the expansion end 112 is located below and the long-strip-shaped crack 111 is located above, the cell cryopreservation tube in the long-strip-shaped crack 111 is easy to slide to the expansion end 112, so after the cell cryopreservation tube slides from the expansion end 112 to the long-strip-shaped crack 111, the expansion end 112 is sealed by the cover body 114, which can be sealed within a certain range or sealed within a larger range, and the cell cryopreservation tube in the long-strip-shaped crack 111 is not easy to slide to the expansion end 112 and fall.

In the present invention, the cover 114 may be of a size that is completely compatible with the shape of the enlarged end 112, as shown in fig. 2-6; may also be sized to fit the shape of the enlarged end 112; and the like. The cover body 114 may be independent or may be connected to a plate body, for example, a connector is disposed between the cover body 114 and the plate body, one end of the connector is connected to the cover body 114, and the other end is connected to the plate body, and the part connected to the plate body may be rotatably connected to the plate body, so that the cover body 114 is convenient to operate; it is also possible that the portion of the connecting body connected to the cover body 114 may be rotatably connected so that the cover body 114 may be conveniently handled when in use.

In the utility model, the support body is used for placing and fixing the cell resuscitation plate 110, and simultaneously, the cell freezing tube on the cell resuscitation plate 110 is required to contact the water with the specific temperature in the tank body 100, the support body can be arranged in various ways, such as a mechanical arm, and the mechanical arm can only clamp one side or two sides of the cell resuscitation plate 110; the support body can also be of other structures.

As in some possible embodiments, the support is at least one upright structure;

the upright post structure is provided with a movable blocking object, the cell recovery plate 110 is provided with a through hole 113 matched with the upright post structure, and the through hole 113 of the cell recovery plate 110 penetrates into the upright post structure and stops at the blocking object.

In some embodiments, the support body may be a column structure, and if the support body is a column structure, a suitable through hole 113 is generally formed in the middle of the cell resuscitation plate 110, and a movable blocking member is disposed on the column structure, so that the cell resuscitation plate 110 is inserted into the column structure and then fixed; in some embodiments, the support body may be two upright structures, and the upright structures are both provided with movable stoppers, at this time, the two sides of the cell resuscitation plate 110 may be provided with suitable through holes 113, and then the corresponding through holes 113 of the cell resuscitation plate 110 are inserted into the upright structures and fixed; in some embodiments, the support body may be a three-column structure, and similarly, the cell recovery plate 110 is provided with three corresponding through holes 113, and then the corresponding through holes 113 of the cell recovery plate 110 are inserted into the three-column structure and fixed; in some embodiments, the support body may be a four-pillar structure, and similarly, the cell resuscitation plate 110 is provided with four corresponding through holes 113, and then the corresponding through holes 113 of the cell resuscitation plate 110 are inserted into the four-pillar structure and fixed; and so on.

The utility model discloses in, the mobilizable obstacle that the stand structure was last to be set up, like in some embodiments, the stand structure is provided with the screw thread along its length, is provided with the screw thread that suits with the screw thread of stand structure on the obstacle, obstacle and stand structure threaded connection. I.e. the position of the obstacle is controlled by its connection to the column structure. Generally, the blocking object is a sheet or a solid object with threads in the middle, so long as the cell resuscitation plate 110 is limited in position and the cell resuscitation plate 110 is kept fixed while the support reciprocates.

In some possible embodiments, the support body is a pillar structure, and the middle of the cell resuscitation plate 110 is provided with a through hole 113 corresponding to the pillar structure;

the through hole 113 of the cell recovery plate 110 penetrates the pillar structure to stay at the barrier and then is fixed by a fixture.

When the support body is of a column structure, the cell recovery plate 110 penetrates through the column structure through the through hole 113 and stays at a barrier, if the cell recovery plate 110 is too large or the sample is unevenly distributed, the cell recovery plate 110 is easy to shake, and the cell recovery plate 110 is further fixed to prevent the situation from happening, so that the through hole 113 of the cell recovery plate 110 penetrates through the column structure and stays at the barrier and is fixed by a fixing object. The fixing object can be a sheet or a solid object with threads in the middle, and the cell recovery plate 110 is fixed between the two objects through the threaded connection.

The utility model discloses in, the motor provides power for drive platform's straight reciprocating motion, and straight reciprocating motion's speed then can be through the rotational speed adjustment of control motor, if in some possible embodiments, the controller that is used for control motor rotational speed is still connected to the motor, and the corresponding adjusting part who sets up control motor rotational speed of operating panel 200 of cell resuscitation water bath.

The utility model provides a cell resuscitation water bath still is provided with some corresponding parts in the aspect of the water bath, like water level information, temperature information, outlet (water pipe 300, as shown in figure 1), switch, refrigeration or heating device etc. correspondingly, sets up corresponding operating button on cell resuscitation water bath's the operating panel 200.

As in some possible embodiments, a water level sensor and a temperature sensor are arranged in the tank body 100, and a temperature display screen and a water level display screen are respectively arranged on the operation panel 200 of the cell resuscitation water bath.

Further, in some possible embodiments, a heater is disposed in the tank body 100, and the operation panel 200 of the cell resuscitation water bath is provided with a temperature regulator and a temperature comparator;

The utility model discloses a straight reciprocating motion mechanism adopt present commercially available straight reciprocating motion module or straight reciprocating motion workstation can, the electricity that relates to be connected carry out corresponding connection according to the conventional means of technical in the field can.

The utility model discloses in, long form crack 111 can be the rectangle, also can be in the rectangular right angle for the condition of fillet.

In some possible embodiments, the number of the elongated slits 111 provided in the plate body may be 1, or may be 2 (see fig. 2-5), 3 (see fig. 6), 4, 5, 6, or the like.

In some possible embodiments, the elongated slits 111 may be disposed on the plate body in various manners, for example, the elongated slits 111 may be disposed at a position near the middle of the plate body, may be distributed at the middle, may be distributed at the edge portion of the plate body, may be disposed at the edge portion of the plate body, or may be randomly distributed on the plate body.

In some possible embodiments, when there are a plurality of the elongated slits 111, the elongated slits 111 may be disposed in various ways, such as parallel arrangement between two elongated slits 111, or intersecting arrangement, such as perpendicular arrangement and intersection at other angles. Of course, there is generally no intersecting portion between the elongated slits 111, and only the extension lines of the elongated slits 111 have an intersecting relationship.

In some possible embodiments, at least two elongated slits 111 are provided on the plate body, and the elongated slits 111 are arranged in parallel (as shown in fig. 2 to 6).

The parallel elongated slits 111 may be equal in length or different in length.

Further, the ends of the elongated slits 111 are provided as the expanded ends 112, and the expanded ends 112 of different elongated slits 111 may be provided at the same end (as shown in fig. 2 to 4) or at different ends (as shown in fig. 5 to 6). In addition, the expanded end 112 may be provided at either end of the elongated slit 111, or the expanded ends 112 may be provided at both ends.

Specifically, in some possible embodiments, one end of the elongated slit 111 is provided as an enlarged end 112, and the same end of different elongated slits 111 is provided with the enlarged end 112.

Because the specification of cell cryopreserving pipe can have some differences, consequently, in order to make the cell cryopreserving pipe of different width melt, the width of the long strip crack 111 of a plurality of rectangular form fissures 111, different rectangular form fissures 111 that sets up on the plate body is different. Of course, if the specifications of the cell cryopreservation tubes are consistent, the widths of the different elongated slits 111 may be set to be the same.

In some possible embodiments, at least two elongated slits 111 are provided on the plate body, and the widths of the different elongated slits 111 are the same or different.

If the plate body is provided with more than two strip-shaped cracks 111, the width of the strip-shaped cracks 111 can be different or partially different.

For example, in some possible embodiments, two elongated slits 111 are provided on the plate body, and the two elongated slits 111 have different widths; in some possible embodiments, two elongated slits 111 are provided on the plate body, and the width of the two elongated slits 111 is the same; in some possible embodiments, three elongated slits 111 are provided on the plate body, and the widths of the three elongated slits 111 may be all different or may be partially different; in some possible embodiments, three elongated slits 111 are provided on the plate body, and the widths of the three elongated slits 111 are the same; by analogy, the above description is omitted.

In the present invention, the gap of the expansion end 112 can be various shapes, such as a square, a rectangle, a pentagon, a hexagon, an octagon, an ellipse, and a circle; and so on.

For example, in some possible embodiments, the slot of the enlarged end 112 is configured to be circular.

In the utility model, the shape of the plate body can be set into various shapes, such as common square, rectangle (figure 2, figure 4-6), pentagon, hexagon, octagon, ellipse and circle (figure 3); and so on.

The utility model discloses in, the material of plate body can be made for materials such as plastics, metal, foam, and the material of plate body is waterproof, and indeformable basically to it is great to prevent to lead to the difference in height that different samples deepened the water bath owing to warp in the water-bath.

In the present application, the terms "first", "second", "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance; the term "plurality" means two or more unless expressly limited otherwise. The terms "mounted," "connected," "fixed," and the like are to be construed broadly, and for example, "connected" may be a fixed connection, a removable connection, or an integral connection; "coupled" may be direct or indirect through an intermediary. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In the description of the present invention, it should be understood that the terms "upper", "lower", "left", "right", "front", "back", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or unit indicated must have a specific direction, be constructed and operated in a specific orientation, and therefore, should not be construed as limiting the present invention.

In the description of the present specification, the description of the terms "some possible implementations," "some embodiments," "specific embodiments," etc. means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A cell resuscitation water bath is characterized by comprising a bath body, wherein a platform driven by a motor is arranged in the bath body;

2. The cell resuscitation water bath according to claim 1, wherein said support is at least one upright structure;

3. The cell resuscitation water bath according to claim 2, wherein said stud structure is provided with a thread along its length, and said barrier is provided with a thread adapted to said stud structure thread, said barrier being in threaded connection with said stud structure.

4. The cell recovery water bath tank as claimed in claim 3, wherein the support body is a column structure, and a through hole matched with the column structure is arranged in the middle of the cell recovery plate;

5. The cell recovery water bath tank as claimed in claim 1, wherein the motor is further connected with a controller for controlling the rotation speed of the motor, and the operation panel of the cell recovery water bath tank is correspondingly provided with a regulating component for controlling the rotation speed of the motor.

6. The cell recovery water bath tank as claimed in claim 1, wherein a water level sensor and a temperature sensor are arranged in the tank body, and a temperature display screen and a water level display screen are correspondingly arranged on an operation panel of the cell recovery water bath tank.

7. The cell recovery water bath tank as claimed in claim 6, wherein a heater is arranged in the tank body, and an operation panel of the cell recovery water bath tank is provided with a temperature regulator and a temperature comparator;

8. The cell recovery water bath according to any one of claims 1-7, wherein at least two elongated slits are provided in the plate body, the elongated slits having at least one different width, the elongated slits being arranged in parallel.

9. The cell recovery water bath according to claim 8, wherein one end of the elongated slit is provided as an enlarged end, and the same end of a different elongated slit is provided with an enlarged end.

10. The cell resuscitation water bath according to claim 8, wherein the aperture of the enlarged end is rounded.