CN213895831U - Pipe cap structure for nucleic acid detection reaction pipe and nucleic acid detection reaction pipe - Google Patents

Abstract

The utility model discloses a pipe cap structure for a nucleic acid reaction detection pipe and a nucleic acid detection reaction pipe, which comprises a main pipe, a pipe cap and a pipe column; one end of the pipe column is positioned at the inner side of the pipe cap, and the other end of the pipe column is provided with a bowl-shaped structure; the side with large area of the bowl-shaped structure is an open structure and faces to the lower end of the main pipe; the length of the pipe column is less than that of the main pipe, and the bowl-shaped structure and the bottom of the main pipe form a closed inner cavity. Under the existing condition, after the reaction reagent is added into the reaction tube, the residual liquid is centrifuged to the tube bottom for reaction after uniform mixing is needed during oscillation. The utility model has the advantages that through the bowl mouth structure, the residual tube wall is greatly reduced when the reagent is mixed, and the centrifugation is saved; for nucleic acid reaction, the risk of aerosol pollution after amplification can be effectively reduced; meanwhile, amplification necessary components such as magnesium ions can be independently added to the bottom of the bowl, good mixing effect can be achieved through small-amplitude shaking and uniform mixing, centrifugation is avoided, hot-start polymerization amplification reaction is achieved, and meanwhile necessary conditions are provided for high-throughput automatic reaction.

Description

Pipe cap structure for nucleic acid detection reaction pipe and nucleic acid detection reaction pipe

Technical Field

The utility model belongs to a biological reaction device, and relates to a nucleic acid detection reaction tube.

Background

Polymerase Chain Reaction (PCR) is a highly sensitive nucleic acid amplification technique with the advantages of: the method has the advantages of high sensitivity, strong specificity, high yield, good repeatability, rapidness, simplicity and the like, is widely applied to the fields of microbiology, archaeology, forensic medicine, sports and the like, is popularized to a plurality of common laboratories, and greatly simplifies the traditional molecular cloning technology, thereby easily analyzing and identifying target genes.

However, the method has the disadvantages that the operation processes of sample preparation (nucleic acid extraction), nucleic acid amplification, amplification product detection and the like need to be carried out separately, the operation steps required by professional operators are more and more complicated, the excessive manual operation is easy to cause cross contamination of samples, contamination of amplification products and other nucleic acid fragments, false positive of detection results and the like. These restrict the use of PCR in clinical assays; secondly, PCR requires continuous temperature change to amplify nucleic acid, so that a thermal cycler is an indispensable device. However, the thermal cycler has a large volume and a high price, so that the popularization and field detection of the thermal cycler in the basement layer are greatly limited.

Thus, there are presented in succession different isothermal amplification techniques, such as Strand Displacement Amplification (SDA) (Walker GT, Little MC, Nadeau JG, et al. Proceedings of the National Academy of Sciences of the United States of America, 1992, 89 (1): 392-, clinical Chemistry, 2005, 51 (10): 1973-1981.), Recombinase Polymerase Amplification (RPA) (Piepenburg O, Williams CH, Stemple DL, Ares NA. PLoS biol. 2006; 4: e 204.), and the like. Researchers have further developed visual detection methods for isothermal amplification technology such as real-time fluorescence, immunoassay, turbidimetric assay, colorimetric assay, visual fluorescent dye assay, and the like.

Compared with the traditional PCR, the technology has single reaction temperature, is not limited by a thermal cycler, and is gradually becoming the best alternative method for PCR. It has played an important role in different fields of clinical medicine, inspection medicine, molecular biology, genomics, food safety and the like, particularly in the detection of microorganisms such as pathogenic bacteria, viruses and the like. However, isothermal amplification techniques also have significant disadvantages, such as tedious pretreatment, complex reaction system, multiple primers and enzymes, and single detection.

Both detection methods have the common disadvantage that the reagents are easily contaminated. The main reasons of pollution are cross contamination among samples, pollution among samples is caused by pollution of a sample suction gun in the extraction process of a sample nucleic acid template, some microorganism samples, particularly viruses, can be diffused along with aerosol or form aerosol, the reaction tube is shaken violently during operation, and aerosol can be formed during uncovering, sample suction and repeated sample suction of a pollution sample injection gun, so that mutual pollution is caused.

The PCR tube has a simple structure, but the frequent use of a pipette and a switch cover is required for detection, which causes the above-described contamination. CN2018218308788 improves the temperature rise and fall speed in the PCR reaction, but does not solve the pollution generated by aerosol and the like; CN2017200068520 uses a simple solid surface sealing reaction solution to achieve the purpose of reducing pollution, but it has the disadvantages that a specific tool is needed for sample addition and reaction triggering to destroy the sealing layer, the reaction space is too small to be mixed evenly, the reaction system is stored in a liquid form, the storage time is short, and the like; CN2018109797262 seals and stores enzymes required for reaction in a reaction system, which can solve the disadvantage of short storage time of the enzymes in the reaction system to a certain extent, but adds more steps for releasing the enzymes in the use of a reaction tube, thereby increasing the uncontrollable property of the reaction, and the enzymes are sealed and still exist in the reaction liquid, or are accidentally released to cause the possibility of detection failure; other kinds of single-tube nucleic acid reaction tubes, such as the diagnostic test reaction tube mentioned in AXXIN corporation, are divided into a plurality of parts, a sample processing tube, a stirring device, a sealing layer and a reaction tube, wherein the reaction tubes with different shapes are required to be selected according to different requirements, and special instruments are required for reading results.

Therefore, a new nucleic acid detection reaction tube needs to be developed, and the purposes of one-time sample introduction, pollution avoidance and no need of centrifugation are met.

Disclosure of Invention

An object of the utility model is to provide a nucleic acid detects for reaction tube pipe cap structure and nucleic acid detects reaction tube, not only can reduce the complexity of operation, can also effectively prevent the pollution problem in the testing process.

The technical scheme of the utility model:

the nucleic acid detection reaction tube is of a tube cap structure and comprises a tube cap and a tube column; one end of the pipe column is positioned at the inner side of the pipe cap, and the other end of the pipe column is provided with a bowl-shaped structure; the side with large area of the bowl-shaped structure is of an open structure.

The nucleic acid detection reaction tube comprises a main tube, a tube cap and a tube column; one end of the pipe column is positioned at the inner side of the pipe cap, and the other end of the pipe column is provided with a bowl-shaped structure; the side with large area of the bowl-shaped structure is an open structure and faces to the lower end of the main pipe; the length of the pipe column is less than that of the main pipe.

In the utility model, the related position is the position relation in practical use, and the side with large bowl-shaped structure area faces downwards; the bottom of being responsible for is called the reaction liquid cavity for depositing the cavity that the reaction liquid is used for the reaction, the utility model discloses bowl form structure's lower surface is gapped with the reaction liquid cavity upper surface of being responsible for, can not influence the reaction like this and go on, for example, bowl form structure's lower surface is apart from the reaction liquid cavity upper surface of being responsible for 0.5mm ~ 8 mm.

In the utility model, a mutually matched sealing structure is arranged between the pipe and the pipe cap, the design can be a conventional fastening structure, for example, the outer side of the upper end part of the main pipe is provided with an external thread, the pipe cap is provided with a matched internal thread, and the internal thread and the external thread are screwed to realize the sealing combination of the main pipe of the pipe cap; or the outer side of the upper end part of the main pipe is provided with a bayonet, the pipe cap is provided with a matched buckle, and the combination of the bayonet and the buckle realizes the sealing combination of the main pipe of the pipe cap.

In the utility model, the length of the pipe column comprises the length of the bowl-shaped structure and is less than the length of the main pipe, so that the pipe column and the lower end part of the main pipe form a sealing structure for the reaction cavity; further, bowl form structure edge and the contact of being responsible for the inner wall, bowl form structure and the reaction chamber of being responsible for the tip down constitute airtight space, combine the pipe cap and be responsible for sealed, form double seal, effectively avoid nucleic acid reaction to receive the pollution, this design does the utility model discloses the initiative.

The utility model discloses in, the lower tip cross-sectional area of being responsible for diminishes gradually, the position that the cross-sectional area begins to diminish does not have the special limitation, technical personnel in the field are according to reaction volume conventional design, it is preferred, the bowl form structure edge is located the position below that the lower tip cross-sectional area of being responsible for begins to diminish with the contact surface of being responsible for the inner wall, can enough play sealed effect like this, reduce when reaction liquid rocks and hinder, also make reaction liquid flow through bowl form structure's upper surface inner wall more easily, dissolve reaction starting material, and when having solved prior art mixing, the problem of reaction liquid glue on the main pipe wall.

The area of the lower surface of the bowl-shaped structure is smaller than or equal to the maximum cross section area of the main pipe, and when the bowl-shaped structure is in close contact with the inner wall (preferably the position with the reduced cross section area) of the main pipe to seal the reaction cavity, the storage and the release of reaction starting materials can be facilitated. The effect of bowl form structure is to form in close contact with the person in charge inner wall to at the person in charge bottom formation airtight inner chamber structure, for nucleic acid detection reaction provides the space, the side inner wall of preferred bowl form structure is the arc, and the less surface of area is upwards, and the opening is towards being responsible for the bottom, and the upper surface inboard of bowl form structure can be plane, cambered surface etc.. The utility model discloses in, the position when spatial position is nucleic acid detection reaction tube in-service use.

Preferably, the tubular column is hollow structure, can lighten weight, and pipe cap, tubular column, bowl-shaped structure can be integrated into one piece structure, also can tubular column, bowl-shaped structure be integrated into one piece structure and then with the conventional equipment of pipe cap, for example bond. The upper surface of the bowl, i.e. the small area surface, may be the lower surface of the column, which is a closed structure for storing the reaction initiating substance.

Preferably, bowl-shaped structure's inner wall is spherical structure, can understand, the utility model discloses a nucleic acid detects each part of reaction tube all has conventional thickness, just also has the inner wall outer wall to contact reaction liquid or be the inner wall towards one side of reaction liquid, spherical structure makes reaction liquid rock the time-wise smooth, dissolves reaction starting material more easily.

When the pipe cap and the main pipe are closed and screwed or fastened, the bowl-shaped structure is matched with the inner wall of the main pipe, so that a closed inner cavity is formed. Single tube nucleic acid reaction can be sealed required reagent in advance and deposit the utility model discloses in, reagent pollution risk is reduced, all reactions concentrate on a detect reagent intraductally go on during the use, and the reaction system can be enlargied as required, increases the accuracy, can add reaction starting material, control reaction time as required in the bowl structure. The reaction system is uniformly mixed, bubbles cannot be generated to influence the reaction, pollution is not easily caused in the detection process, cross pollution cannot occur, and the detection precision is higher. The bowl-shaped structure can be added with different substances according to different requirements, can be freeze-dried and attached, can be sealed by other substances and can be released when the reaction is needed. Compared with the existing reaction tube, the utility model has the advantages of simple structure, simplified operation, wide application range, etc.

The utility model discloses a nucleic acid detects reaction tube, adopt reaction system material to separately seal up and deposit, "bowl" form cock body seals up and deposits materials such as reaction start, is responsible for the bottom and seals up and deposits other essential material (reaction liquid), seals up and deposits the optional freeze-drying of but not limiting on of method, wax sealing etc. only need add during the detection and dissolve liquid and sample, screws up or straining reaction tube, can begin to react after the mixing. When the detection device is used, all reactants are concentrated in one detection reagent tube, the reaction controllability is good, the detection can be finished only by opening the cover once, the pollution and the cross pollution can be effectively avoided, the detection reagent of the dissolving solution can be adjusted according to the existing conditions, the detection reagent can be selected from but not limited to DNA dyeing, a fluorescent probe and the like, and the detection precision is improved.

In the prior art, after a reaction reagent is added into a reaction tube, the reagent is required to be uniformly mixed, a part of liquid reagent is remained on the tube wall during mixing, and the remained liquid is required to be centrifuged to the tube bottom for reaction through centrifugation, which is a problem objectively existing in the prior art. The utility model forms a closed space required by detection reaction with the reagent tube by the bowl mouth structure extending into the lower part of the tube part, thereby greatly reducing the tube wall remained when the reagent is mixed uniformly, saving the centrifugation step and improving the operation convenience; the double-sealing structure can effectively reduce the expanded aerosol pollution caused by untight sealing; meanwhile, certain components necessary for amplification, such as magnesium ions, can be prefabricated on a bowl opening in advance and dried in the air, after a pipe cover is screwed or fastened, automatic mixing can be achieved through an automatic mixing device with small amplitude, centrifugation is avoided, and therefore physical hot start polymerization amplification reaction is achieved, and necessary conditions are provided for high-throughput automatic reaction.

Drawings

FIG. 1 is a schematic diagram of a main pipe structure according to an embodiment;

FIG. 2 is a schematic view of a cap according to an embodiment;

FIG. 3 is a schematic structural view of a second embodiment of a cap;

FIG. 4 is a schematic structural view of a triple cap according to an embodiment;

FIG. 5 is a schematic diagram of a four-tube cap according to an embodiment;

FIG. 6 is a schematic diagram showing the structure of a nucleic acid detecting reaction tube according to the second embodiment;

FIG. 7 is a schematic structural view of a five-tube cap according to an embodiment;

FIG. 8 is a schematic structural view of a six-main tube and a tube cap according to an embodiment;

the pipe comprises a main pipe 1, a pipe cap 2, a pipe column 3, a bowl-shaped structure 4, an external thread 5, an internal thread 6, a spherical structure 7, an arc-shaped structure 8, a protrusion 9, a bayonet 10 and a buckle 11.

Detailed Description

Each part of the nucleic acid detection reaction tube has conventional thickness, one side contacting with the reaction liquid or facing to the reaction liquid is taken as an inner wall, and the spatial position is the position of the nucleic acid detection reaction tube in actual application; the specific size of each component is selected according to the actual reaction liquid amount, and generally the components are in a regular structure. The utility model discloses a creativity lies in setting up the tubular column that has bowl form structure in the pipe cap inboard, utilizes bowl form structure to play and deposits reaction starting material, double sealing, avoids the reaction solution to glue technical effect such as being responsible for the inner wall. The present invention will be further described with reference to the drawings and examples, but the invention is not limited thereto; the specific bowl-shaped structure, internal and external threads, and bayonet catches can all be prepared according to conventional methods in the art.

Example one

Referring to FIGS. 1-2:

the nucleic acid detection reaction tube consists of a main tube 1, a tube cap 2 and a tube column 3, wherein the tube cap and the tube column form a tube cap structure for the nucleic acid detection reaction tube; the cross section area of the lower end part of the main pipe is gradually reduced, and the bottom part of the main pipe is a reaction liquid cavity; one end of the pipe column is positioned at the inner side of the pipe cap, the other end of the pipe column is provided with a bowl-shaped structure 4, and the side with the large area of the bowl-shaped structure is an open structure and faces the lower end of the main pipe; the length of the pipe column is smaller than that of the main pipe, the length of the pipe column comprises the length of a bowl-shaped structure and is smaller than that of the main pipe, and thus the pipe column and a reaction cavity at the lower end part of the main pipe form a sealing structure;

the outer side of the upper end part of the main pipe is provided with an external thread 5, and the pipe cap is provided with an internal thread 6; the external thread is matched with the internal thread, so that the pipe cap can be screwed with the main pipe to achieve a sealing effect;

the bowl-shaped structure is in close contact with the inner wall of the main pipe, so that a closed inner cavity structure is formed at the bottom of the main pipe to provide a space for nucleic acid detection reaction, and the opening of the bowl-shaped structure faces the bottom end of the main pipe; bowl form structure's lower surface area is less than the biggest cross sectional area of being responsible for, and the bowl form structure edge is located the position below that the lower tip cross sectional area of being responsible for begins to diminish with the contact surface of being responsible for the inner wall, can enough play sealed effect like this, reduces the hindrance when reaction liquid rocks, also makes reaction liquid flow through bowl form structure's upper surface inner wall more easily, dissolves reaction starting material, can also avoid reaction liquid to glue the inner wall of being responsible for, has solved the centrifugation step that prior art all exists.

Bowl form structure edge and the interior wall contact of being responsible for, the airtight space of reaction chamber that bowl form structure and the tip of being responsible for are constituteed combines the pipe cap and is responsible for sealed, forms double seal, effectively avoids nucleic acid reaction to receive the pollution, and this design does the utility model discloses the initiative.

The utility model discloses in, the lower tip of being responsible for, the position that the cross-sectional area begins to diminish is not specially limited, as nucleic acid reaction cavity, and the technical staff in the field designs according to the reaction volume conventionality. The bowl-shaped structure is in close contact with the inner wall (with reduced cross-sectional area) of the main pipe to seal the reaction cavity, and meanwhile, the storage and release of reaction starting materials can be facilitated. The pipe column is of a hollow structure, so that the weight can be reduced, and the pipe cap, the pipe column and the bowl-shaped structure are of an integrally formed structure; the upper surface of the bowl-shaped structure, i.e. the inner wall of the surface with small area, is a closed structure and is used for storing reaction starting materials.

Example two

On the basis of the first embodiment, the inner wall of the bowl-shaped structure is a spherical structure 7, and the rest is unchanged, see fig. 3.

EXAMPLE III

On the basis of the first embodiment, the side inner wall of the bowl-shaped structure is arc-shaped 8, similar to an inverted bowl, and the rest is unchanged, see fig. 4.

It can be understood that the utility model discloses a nucleic acid detects each part of reaction tube all has conventional thickness, just also has the inner wall outer wall to contact reaction liquid or be the inner wall towards one side of reaction liquid, spherical surface structure or arc structure make reaction liquid rock the time-wise smooth, dissolve reaction starting material more easily.

Example four

On the basis of the first embodiment, the second embodiment or the third embodiment, the protrusions 9 are arranged on the outer side of the upper end part of the main pipe and below the external thread, and the rest is not changed, which is shown in fig. 5.

The tube cap and the main tube are screwed, and the tube column is inserted into the main tube to form the nucleic acid detection reaction tube, referring to fig. 6, the schematic diagram of the second embodiment is taken as an example, and the schematic diagram of the first embodiment and the third embodiment is referred to as the example.

EXAMPLE five

On the basis of the second embodiment, the contact surface of the edge of the bowl-shaped structure and the inner wall of the main pipe is positioned above the position where the cross-sectional area of the lower end part of the main pipe begins to become smaller, and the rest is unchanged, see fig. 7.

Coating a reaction starting material on the inner wall of the upper surface of the bowl-shaped structure, adding a reaction liquid to the bottom of the main pipe, and then screwing the pipe cap and the main pipe, and freezing or not freezing; when nucleic acid detection reaction is required, if the nucleic acid is frozen, the nucleic acid is thawed, the tube cap is opened, and a detection sample is added; then twist the pipe cap with the person in charge, rock the reaction liquid gently, the reaction liquid of bowl structure's upper surface inner wall of flowing through can dissolve reaction starting material, through the actual measurement (ten parallel experiments of every structure, the reaction liquid that all experiments adopted, reaction starting material quantity unanimous), the utility model provides an one to the fifth nucleic acid of embodiment detect reaction tube, only need rock slightly (rock angle 30 or vortex vibrations 2-6 seconds) can all dissolve reaction starting material rapidly, open promptly and see, bowl structure's upper surface inner wall does not have any reaction starting material to remain, wherein embodiment rocks for 6 times, embodiment two rocks 4 times, embodiment three rocks 4 times, embodiment five rocks 10 times.

EXAMPLE six

On the basis of the first embodiment, the outer side of the upper end part of the main pipe is provided with a bayonet 10, and the pipe cap is provided with a buckle 11; the bayonet 10 is matched with the buckle 11, so that the pipe cap can be fastened with the main pipe to play a sealing effect, and the rest is unchanged, see fig. 8.

Application examples

During practical application, reaction liquid (the bottom of a main pipe) and reaction starting materials (the inner wall of the upper surface of a bowl-shaped structure) are already stored in a nucleic acid detection reaction pipe purchased by a detection mechanism, a pipe cap is opened during detection, a sample is added, the pipe cap is screwed or fastened, an automatic mixing machine is uniformly mixed or shaken by hand, and conventional amplification reaction can be carried out without centrifugation.

The nucleic acid detection reaction tube of example two was used for HDA reaction amplification of target sequences, and experimental condition references were made, An L, Tang W, Ranalli TA, Kim HJ, Wytiaz J, Kong H.J Biol chem.2005; 280(32): 28952-.

The bottom of the main channel contains the reaction solution including 1 XThermoPol II buffer, 200. mu.M dNTPs, 3mM dATP, 120 units of Bst polymerase, 600ng of Tte-UvrD helicase, 1.2mg of Tte-MutL, 1 × sybr green, 100000 copies of DNA template, water to make up ddH2O to a final reaction volume of 300 μ l, 250nM final concentration of each primer adhering to the inside of the bowl top surface, 3.5 mM final concentration of MgSO 2₄。

Opening the tube cap, adding the template, covering the tube cap, shaking the automatic mixer for four times at 30 degrees without centrifugation, immediately detecting the change value of fluorescence by using a fluorescence detector capable of placing a large centrifuge tube, reading the fluorescence every 30s, and reacting for 25 minutes at 65 ℃.

By combining all the elements described above in the reaction tube of the present invention and incubating directly at 65 ℃ for 25 minutes, a HDA reaction without thermal denaturation can be established.

Finally, the sensitivity of the method is confirmed to be capable of detecting 50 copies through detection, and the sensitivity can completely meet the application requirement.

Comparative example

The main pipe of the second embodiment is combined with a pipe cap without a pipe column as a comparative example, and it can be understood that a bowl-shaped structure does not exist; when the same nucleic acid amplification experiment is carried out, multi-step sample adding is needed, particularly centrifugal treatment is needed after uniform mixing, otherwise reaction liquid adhesion is obviously seen on the tube wall; and the lower surface of two bowl-shaped structures of embodiment is apart from the reaction liquid upper surface 1mm who is responsible for, and it is not centrifugal also not have the reaction liquid to bond, and further, when bowl-shaped structure's lower surface is apart from the reaction liquid upper surface 2mm ~ 5mm who is responsible for, it is not centrifugal also not have the reaction liquid to bond, explains the utility model discloses a bowl-shaped structure back-off can avoid the problem that the interior wall bonding of being responsible for that prior art rocked and leads to remains the reaction liquid.

Through conventional experiment, the testing result of finding the second test tube of embodiment is superior to the comparative example, and this demonstrates the utility model discloses not only the application of sample is simple and convenient, and an application of sample can be accomplished, need not the centrifugation moreover, and most important, double sealing has improved the effect that detects.

The third embodiment of the nucleic acid detection reaction tube is used for detecting the prawn baculovirus

The reaction solution is stored at the bottom of the main tube and includes 60mM Tris-acetate buffer (pH8.0), 100mM potassium acetate, 3mM dithiothreitol, 5% polyethylene glycol (20000), 2mM ATP, 20mM creatine phosphate, 420nM primer mixture, 120nM fluorescent probe, 100 ng/. mu.l creatine kinase, 600 ng/. mu.l phage gp32 protein, 150 ng/. mu.l phage uvsX protein, 25 ng/. mu.l phage uvsY protein, 80 ng/. mu.l klenow polymerase large fragment (exo-), 50 ng/. mu.l exonuclease III, 450. mu.l dNTP primer mixture (50 mu.l system)

The magnesium acetate with a final concentration of 14mM is adhered to the inner side of the upper surface of the bowl-shaped structure

The tube cap was opened, the template was added, the tube cap was closed, the automatic mixer was shaken four times at 30 ℃ without centrifugation, and then the change in fluorescence was immediately detected with a fluorescence constant temperature amplification apparatus (model GS8, dada technologies, su), the fluorescence was read every 30s, and the reaction was carried out at 37 ℃ for 20 minutes.

Finally confirming that the sensitivity can be detected by 10 after detection^-7ng/mu L, the sensitivity can completely meet the application requirement.

Comparative example

The main pipe of the third example is combined with a pipe cap without a pipe column as a comparative example, and it can be understood that a bowl-shaped structure does not exist; when the same nucleic acid amplification experiment is carried out, multi-step sample adding is needed, particularly centrifugal treatment is needed after uniform mixing, otherwise reaction liquid adhesion is obviously seen on the tube wall; and the lower surface of three bowl-shaped structure of embodiment is apart from the reaction liquid upper surface 1mm who is responsible for, and it is not centrifugal also not have the reaction liquid to bond, and further, when bowl-shaped structure's lower surface was apart from the reaction liquid upper surface 2mm ~ 5mm who is responsible for, it was not centrifugal also not to have the reaction liquid to bond, explains the utility model discloses a bowl-shaped structure back-off can avoid the problem that the interior wall bonding of being responsible for that prior art rocked and leads to remains the reaction liquid.

Through conventional experiment, the testing result of finding three sense tubes of embodiment is superior to the comparative example, and this demonstrates the utility model discloses not only the application of sample is simple and convenient, and an application of sample can be accomplished, need not the centrifugation moreover, and most important, double sealing has improved the effect that detects.

Claims (8)

1. The pipe cap structure for the nucleic acid detection reaction pipe is characterized by comprising a pipe cap and a pipe column; one end of the pipe column is positioned at the inner side of the pipe cap, and the other end of the pipe column is provided with a bowl-shaped structure; the side with large area of the bowl-shaped structure is of an open structure.

2. The cap structure for nucleic acid detecting reaction tube according to claim 1, wherein the inner wall of the side of the bowl-shaped structure is curved.

3. The cap structure for nucleic acid detecting reaction tube according to claim 1, wherein the inner wall of the bowl-shaped structure is a spherical structure.

4. The nucleic acid detection reaction tube is characterized by comprising a main tube, a tube cap and a tube column; one end of the pipe column is positioned at the inner side of the pipe cap, and the other end of the pipe column is provided with a bowl-shaped structure; the side with large area of the bowl-shaped structure is an open structure and faces to the lower end of the main pipe; the length of the pipe column is less than that of the main pipe.

5. The nucleic acid detecting reaction tube according to claim 4, wherein the area of the lower surface of the bowl-shaped structure is equal to or less than the maximum cross-sectional area of the main tube; the edge of the bowl-shaped structure is contacted with the inner wall of the main pipe.

6. The nucleic acid detecting reaction tube according to claim 4, wherein the inner wall of the side of the bowl-shaped structure is curved.

7. The nucleic acid detecting reaction tube according to claim 4, wherein the inner wall of the bowl-shaped structure is a spherical structure.

8. The nucleic acid detecting reaction tube according to claim 4, wherein a seal structure is provided between the main tube and the cap for engaging with each other.

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