CN213835126U - Protein renaturation device for enzyme linked immunosorbent assay experiments - Google Patents
Protein renaturation device for enzyme linked immunosorbent assay experiments Download PDFInfo
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- CN213835126U CN213835126U CN202022609504.7U CN202022609504U CN213835126U CN 213835126 U CN213835126 U CN 213835126U CN 202022609504 U CN202022609504 U CN 202022609504U CN 213835126 U CN213835126 U CN 213835126U
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Abstract
The utility model discloses a protein renaturation device for enzyme-linked immunosorbent assay experiments, wherein a recovery box is arranged in a working box body; the first container is arranged at the top of the working box body and is connected with a recovery box in the working box body through a liquid discharge pipe; the second container is arranged in the first container, and the second container and the first container are not communicated; a liquid supply pump is arranged in the liquid storage tank, the liquid storage tank is communicated with the second container through a liquid conveying pipe, and the liquid in the liquid storage tank is pumped into the second container through the liquid conveying pipe by the liquid supply pump; the telescopic box body is positioned on one side of the working box body, a telescopic column is fixed at the top of the telescopic box body, a bearing plate is connected onto the telescopic column, a driving motor is arranged at the top of the bearing plate, a stirring shaft is arranged at the position, corresponding to the second container, at the bottom of the bearing plate, and an output shaft of the driving motor is connected with the stirring shaft; the utility model discloses a experimental protein renaturation device of ELISA adsorption method can improve the effect of protein dialysis to can improve stirring efficiency, improve stirring effect, realize the rapidization and the standardization of ELISA experiment.
Description
Technical Field
The utility model belongs to the technical field of ELISA experimental device, in particular to protein renaturation device for ELISA experiments.
Background
In performing an ELISA test on rat interferon gamma (IFN- γ), it is sometimes necessary to first perform protein renaturation on a sample, and to remove a denaturation factor when denaturation conditions are not severe and the internal structure of the denatured protein is not greatly changed, and to restore the native conformation and biological activity of the denatured protein under appropriate conditions, which is called renaturation of the protein. The phenomenon that the spatial conformation of a molecule is destroyed by the influence of certain physical or chemical factors, so that the physicochemical properties of the protein are changed and the original biological activity is lost is called protein denaturation. Denaturation does not cause the destruction of the primary structure of the protein, but the destruction of the secondary structure and higher structures, and the denatured protein is called a denatured protein.
The protein renaturation method is to dialyze protein dialysate according to gradient 8M-6M-5M-4M-3M-2M-1M-PBS, each gradient needs 4-8h, and the method has certain requirement on the solubility of the protein to be renatured, and the general protein concentration is 0.1-1 mg/ml. Because a plurality of gradients are needed, time is calculated manually, solution is prepared, dialysate is continuously replaced, time consuming is long, the concentration of protein to be dialyzed is low, the probability of protein precipitation is high, concentration and other treatment are needed in the later stage, meanwhile, when the solution is prepared, the solution needs to be stirred manually, the condition of uneven stirring can occur, and long-term manual stirring can also lead to high labor intensity of experimenters and low personnel utilization rate.
Based on this situation, the present inventors have made research and improvement, and the present case has been made therefrom.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing an experimental protein renaturation device of ELISA adsorption method, it can improve the effect of protein dialysis to can improve stirring efficiency, improve stirring effect, use manpower sparingly.
In order to achieve the above purpose, the solution of the present invention is:
a protein renaturation device for an enzyme-linked immunosorbent assay experiment comprises a working box body, a recovery box, a first container, a second container, a liquid storage box and a telescopic box body;
a recovery box is arranged in the working box body, and an access door is arranged on the side surface corresponding to the recovery box;
the first container is arranged at the top of the working box body and is connected with a recovery box in the working box body through a liquid discharge pipe; the second container is arranged in the first container, and the second container and the first container are not communicated;
a liquid supply pump is arranged in the liquid storage tank, the liquid storage tank is communicated with the second container through a liquid conveying pipe, and the liquid in the liquid storage tank is pumped into the second container through the liquid conveying pipe by the liquid supply pump;
the telescopic box body is located on one side of the working box body, a telescopic column is fixed at the top of the telescopic box body, a bearing plate is connected onto the telescopic column, a driving motor is arranged at the top of the bearing plate, a stirring shaft is arranged at the position, corresponding to the second container, of the bottom of the bearing plate, and the driving motor is connected with the stirring shaft through an output shaft.
The bottom of the work box body is provided with a support column, and protective paint is sprayed on the surfaces of the work box body and the support column.
And a sealing strip is fixed at the joint of the access door and the working box body.
The liquid level sensor is arranged on the inner wall of the liquid storage tank at a position flush with the liquid supply pump, the controller is arranged on the side face of the working tank body, the liquid level sensor senses the position of liquid in the liquid storage tank and transmits the position to the controller, and when the liquid level sensor exceeds a threshold value, the controller controls the liquid supply pump to be started.
The upper part of the liquid storage tank is provided with a liquid inlet hole.
And sealing rings are arranged at the joints of the infusion tube, the liquid storage tank and the second container.
The telescopic cylinder is arranged in the telescopic box body, the top of the telescopic cylinder is connected with the bottom of the telescopic column, and the top of the telescopic column is connected with the bottom of the bearing plate.
And a sealing ring is arranged at the joint of the liquid discharge pipe and the recovery box.
After the scheme is adopted, the utility model discloses compare prior art's beneficial effect is: this protein renaturation device, setting through driving motor and (mixing) shaft, it can dilute the protein dialysate in succession to reach the device, do not need the manual work to trade the liquid, make the protein renaturation process be in a continuous state, it presents the linearity degressive to guarantee the protein dialysate concentration, be convenient for realize the protein renaturation, it is lower to the requirement of protein concentration simultaneously, setting through liquid reserve tank and industry pump, realize full automatic supply liquid, it dilutes the protein dialysate to provide stable liquid to come in succession, reduce personnel's use, and the device is simple, low cost, easy realization, no matter be laboratory or mill all can use, especially, to the manpower, nervous laboratory, small-size enterprise etc. are especially important.
Drawings
FIG. 1 is a perspective view of the present invention;
FIG. 2 is an internal structure view of the telescopic box of the present invention;
fig. 3 is a schematic view of the internal structure of the middle liquid storage tank of the present invention.
Reference numerals:
1. a working box body; 2. a support pillar;
3. a controller; 4. an access door; 5. a recycling bin;
6. a first container; 7. a second container;
8. a liquid storage tank; 9. a liquid supply pump;
10. a telescopic box body; 11. a telescopic column; 12. a carrier plate;
13. a drive motor; 14. a stirring shaft;
15. a hinge; 16. a liquid level sensor;
17. a liquid inlet hole; 18. a transfusion tube; 19. a telescopic cylinder; 20. and a liquid discharge pipe.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.
As shown in fig. 1 to 3, the utility model provides a protein renaturation device for enzyme-linked immunosorbent assay experiments, which comprises a working box body 1, wherein a support column 2 is fixedly arranged at the bottom of the working box body 1, a controller 3 is fixedly arranged at one side of the working box body 1, an access door 4 is fixedly arranged at the other side of the working box body 1, and a recovery box 5 is fixedly arranged at one side of the access door 4 and in the working box body 1; a first container 6 is fixedly arranged at the top of the working box body 1, and a second container 7 is fixedly arranged in the first container 6; a liquid storage tank 8 is fixedly arranged on one side of the working tank body 1, which is far away from the access door 4, and a liquid supply pump 9 is fixedly arranged in the liquid storage tank 8; work box 1 is keeping away from controller 3 one side fixed mounting and is having flexible box 10, and flexible box 10 top fixed mounting has flexible post 11, and flexible post 11 top fixed mounting has loading board 12, and loading board 12 top fixed mounting has driving motor 13, and driving motor 13 bottom fixed mounting has (mixing) shaft 14.
As a preferred embodiment of the utility model, as shown in fig. 1, the support columns 2 are fixedly provided with four, four support columns 2 are fixedly installed at the bottom of the work box body 1 through spot welding, and the surface of the support columns 2 and the surface of the work box body 1 are all fixedly sprayed with protective paint.
As a preferred embodiment of the present invention, as shown in fig. 1, the access door 4 is fixedly installed on one side of the working box body 1 through a hinge 15, the hinge 15 is fixedly installed on one side of the access door 4 and the working box body 1 through screws, and a sealing strip is fixedly installed at the connection between the access door 4 and the working box body 1.
As a preferred embodiment of the present invention, as shown in fig. 3, a liquid level sensor 16 is fixedly installed inside the liquid storage tank 8 at a side close to the liquid supply pump 9, and the liquid level sensor 16 is electrically connected to the controller 3; a liquid inlet hole 17 is fixedly arranged on one side of the liquid storage tank 8 far away from the liquid level sensor 16, and a liquid conveying pipe 18 is fixedly arranged on one side of the liquid supply pump 9.
As a preferred embodiment of the utility model, the infusion tube 18 runs through the top of the liquid storage box 8 and one side of the first container 6, the infusion tube 18 extends to the inside of the second container 7, and the joints of the infusion tube 18 and the liquid storage box 8, the first container 6 and the second container 7 are fixedly provided with sealing rings.
As a preferred embodiment of the present invention, as shown in fig. 2, a telescopic cylinder 19 is fixedly installed inside the telescopic box 10, the telescopic cylinder 19 is fixedly installed inside the telescopic box 10 through a fixing seat, the top of the telescopic cylinder 19 is connected with the telescopic column 11 through a screw seat, and the telescopic column 11 is fixedly installed at the bottom of the loading plate 12 through a screw seat.
As a preferred embodiment of the present invention, as shown in fig. 1, a liquid discharge pipe 20 is fixedly installed on one side of the first container 6 away from the infusion tube 18, the liquid discharge pipe 20 penetrates through the top of the work box 1 and extends into the recycling box 5, and a sealing ring is fixedly installed at the connection position of the liquid discharge pipe 20 and the recycling box 5.
As a preferred embodiment of the present invention, as shown in fig. 1, the output end of the driving motor 13 is connected to the stirring shaft 14 through a coupling, and the driving motor 13 is electrically connected to the controller 3.
The utility model discloses a theory of operation is: pouring renaturation protein dialysate into the second container 7, controlling a telescopic cylinder 19 in the telescopic box body 10 to start by the controller 3, and driving the telescopic column 11 to move longitudinally by the telescopic cylinder 19; the stirring shaft 14 is placed in the second container 7, the controller 3 controls the driving motor 13 to start, the driving motor 13 drives the stirring shaft 14 to rotate, the liquid supply pump 9 starts to convey liquid, the liquid is input into the second container 7 through the liquid conveying pipe 18 at a constant speed, protein dialysate can be continuously diluted, the effect is better, the liquid is continuously input, the leaked liquid falls into the first container 6, and the leaked liquid is discharged into the recovery box 5 through the liquid discharge pipe 20; through the arrangement of the driving motor 13 and the stirring shaft 14, the protein dialysate can be continuously diluted by the device, manual liquid replacement is not needed, the protein renaturation process is in a continuous state, the concentration of the protein dialysate is ensured to be linearly reduced, the protein renaturation is convenient to realize, and the requirement on the protein concentration is low; through the setting of liquid reserve tank 8 and feed pump 9, realize full-automatic supply liquid, provide stable liquid and dilute the protein dialysate in succession, reduce personnel and use, the device is simple moreover, and is with low costs, realizes easily, no matter be the laboratory or mill all can use, especially important to laboratory, small-size enterprise etc. that manpower, material resources are nervous.
In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description of the present invention and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above should not be understood to necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples described in this specification can be combined and combined by those skilled in the art.
Although embodiments of the present invention have been shown and described, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art without departing from the scope of the present invention.
Claims (8)
1. A kind of enzyme-linked immunosorbent assay experiment uses the protein renaturation apparatus, characterized by that: comprises a working box body, a recovery box, a first container, a second container, a liquid storage box and a telescopic box body;
a recovery box is arranged in the working box body, and an access door is arranged on the side surface corresponding to the recovery box;
the first container is arranged at the top of the working box body and is connected with a recovery box in the working box body through a liquid discharge pipe; the second container is arranged in the first container, and the second container and the first container are not communicated;
a liquid supply pump is arranged in the liquid storage tank, the liquid storage tank is communicated with the second container through a liquid conveying pipe, and the liquid in the liquid storage tank is pumped into the second container through the liquid conveying pipe by the liquid supply pump;
the telescopic box body is located on one side of the working box body, a telescopic column is fixed at the top of the telescopic box body, a bearing plate is connected onto the telescopic column, a driving motor is arranged at the top of the bearing plate, a stirring shaft is arranged at the position, corresponding to the second container, of the bottom of the bearing plate, and the driving motor is connected with the stirring shaft through an output shaft.
2. The protein renaturation device for enzyme linked immunosorbent assay experiments as claimed in claim 1, wherein: the bottom of the work box body is provided with a support column, and protective paint is sprayed on the surfaces of the work box body and the support column.
3. The protein renaturation device for enzyme linked immunosorbent assay experiments as claimed in claim 1, wherein: and a sealing strip is fixed at the joint of the access door and the working box body.
4. The protein renaturation device for enzyme linked immunosorbent assay experiments as claimed in claim 1, wherein: the liquid storage tank inner wall is equipped with the liquid level inductor in the position with the feed pump parallel and level, and the side of work box is equipped with the controller, and the liquid level inductor sensing liquid position in the liquid storage tank conveys to the controller, and when exceeding the threshold value, the controller control feed pump starts.
5. The protein renaturation device for enzyme linked immunosorbent assay experiments as claimed in claim 1, wherein: the upper part of the liquid storage tank is provided with a liquid inlet hole.
6. The protein renaturation device for enzyme linked immunosorbent assay experiments as claimed in claim 1, wherein: and sealing rings are arranged at the joints of the infusion tube, the liquid storage tank and the second container.
7. The protein renaturation device for enzyme linked immunosorbent assay experiments as claimed in claim 1, wherein: the telescopic cylinder is arranged in the telescopic box body, the top of the telescopic cylinder is connected with the bottom of the telescopic column, and the top of the telescopic column is connected with the bottom of the bearing plate.
8. The protein renaturation device for enzyme linked immunosorbent assay experiments as claimed in claim 1, wherein: and a sealing ring is arranged at the joint of the liquid discharge pipe and the recovery box.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022609504.7U CN213835126U (en) | 2020-11-12 | 2020-11-12 | Protein renaturation device for enzyme linked immunosorbent assay experiments |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022609504.7U CN213835126U (en) | 2020-11-12 | 2020-11-12 | Protein renaturation device for enzyme linked immunosorbent assay experiments |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213835126U true CN213835126U (en) | 2021-07-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022609504.7U Active CN213835126U (en) | 2020-11-12 | 2020-11-12 | Protein renaturation device for enzyme linked immunosorbent assay experiments |
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| Country | Link |
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| CN (1) | CN213835126U (en) |
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- 2020-11-12 CN CN202022609504.7U patent/CN213835126U/en active Active
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