CN213792230U

CN213792230U - Centrifugal PCR analyzer for SNP detection

Info

Publication number: CN213792230U
Application number: CN202022661230.6U
Authority: CN
Inventors: 赵建华; 杨燕宇; 侯静; 罗辉
Original assignee: Wuhan Jingpeptide Biotechnology Co ltd
Current assignee: Wuhan Jingpeptide Biotechnology Co ltd
Priority date: 2020-11-17
Filing date: 2020-11-17
Publication date: 2021-07-27
Anticipated expiration: 2030-11-17

Abstract

The utility model discloses a centrifugal PCR analysis appearance is used in SNP detects, involve the PCR analysis appearance field, including the analysis appearance organism, the circular slot has been seted up at analysis appearance organism upper end middle part, the ring channel has been seted up to the circumference lateral wall below of circular slot, the inside fixedly connected with fluorescence detection module of ring channel, the top middle part of analysis appearance organism is rotated through rotary mechanism and is connected with the carousel, circular cavity has been seted up at the inside middle part of carousel, the bull stick of connecting vertical setting is rotated through first bearing in the inside of circular cavity, the fixed cover of pole wall below of bull stick has first bevel gear, the upper end of bull stick extends to the top and the fixedly connected with knob of carousel. The utility model discloses can adjust the position of placing of centrifugal test tube to can adjust the centrifugal force that liquid received when rotating in the centrifugal test tube, improve centrifugal efficiency and centrifugal effect, and be convenient for compress tightly the centrifugal test tube, avoid the test tube to take place to rock and lead to liquid to spill when the centrifugation.

Description

Centrifugal PCR analyzer for SNP detection

Technical Field

The utility model relates to a PCR analysis appearance field, in particular to centrifugal PCR analysis appearance is used in SNP detection.

Background

The SNP suspended biological filler is a spherical filler consisting of a fiber filament sphere, a grid shell and a through porous cylinder, overcomes the defects that the existing soft and semi-soft fillers are difficult to fixedly install, maintain and manage, the soft fillers are easy to form balls and block, the semi-soft fillers are poor in film hanging and the like, and overcomes the defects of high power consumption and small specific surface area of carriers such as quartz sand, ceramsite and the like. Has a uniquely designed aerobic, facultative and anaerobic functional area, can fully and effectively exert the sewage treatment capacity, and has less sludge production which is about 80 percent of an activated sludge system.

At present, after SNP is produced, a centrifugal PCR analyzer is needed to be used for centrifuging and detecting SNP liquid, but in the prior art, the placing position of a test tube in the centrifugal PCR analyzer is fixed, so that the centrifugal force of the SNP liquid in the test tube is not convenient to adjust, the centrifugal effect of the SNP is influenced, and people are not convenient to detect.

Therefore, it is necessary to develop a centrifugal PCR analyzer for SNP detection to solve the above problems.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a centrifugal PCR analysis appearance is used in SNP detects to the test tube among the centrifugal PCR analysis appearance that proposes in solving above-mentioned background art places the position and all is fixed unchangeable, thereby leads to the centrifugal force that the SNP liquid in the test tube received to be not convenient for adjust, influences the centrifugal effect of SNP, the people of being not convenient for carry out the problem that detects.

In order to achieve the above object, the utility model provides a following technical scheme: a centrifugal PCR analyzer for SNP detection comprises an analyzer body, wherein a circular groove is formed in the middle of the upper end of the analyzer body, an annular groove is formed in the lower portion of the circumferential side wall of the circular groove, a fluorescence detection module is fixedly connected inside the annular groove, a rotary disc is rotatably connected to the middle of the upper portion of the analyzer body through a rotating mechanism, a circular cavity is formed in the middle of the inner portion of the rotary disc, the inner portion of the circular cavity is rotatably connected with a vertically arranged rotary rod through a first bearing, a first bevel gear is fixedly sleeved below the rod wall of the rotary rod, the upper end of the rotary rod extends to the upper portion of the rotary disc and is fixedly connected with a knob, a plurality of uniformly distributed strip-shaped openings are further formed in the upper end of the rotary disc, threaded rods are rotatably connected to the inner portions of the strip-shaped openings through second bearings, and second bevel gears are fixedly connected to the ends, close to the circular cavity, of the plurality of the threaded rods, the second bevel gears are all meshed with the first bevel gears and are connected with the rod walls of the threaded rods in a sleeved mode through threads, a cylinder is fixedly connected to one side of the threaded cylinder, a centrifugal test tube is inserted into the cylinder, a pressing mechanism is arranged above the rotary disc, and a protection mechanism is arranged above the analyzer body.

Preferably, rotary mechanism includes buncher, the motor chamber has been seted up to the inside below of analysis appearance organism, buncher is located the fixed setting in inside in motor chamber, and buncher's upper end rotates through the shaft coupling and is connected with the pivot, the upper end of pivot extend to the inside of circular slot and with the lower extreme middle part fixed connection of carousel, the side wall of going up of third bearing and motor chamber through the pivot of pivot rotates and is connected.

Preferably, one side of the cylinder, which is far away from the threaded rod, is fixedly connected with a sliding block, and a sliding groove matched with the sliding block is formed in the side wall of the strip-shaped opening.

Preferably, hold-down mechanism includes transparent pressure strip, the right side of transparent pressure strip is passed through the hinge and is connected with the right side rotation of carousel, the left end fixedly connected with first box fastener of transparent pressure strip, the left end fixedly connected with second box fastener of carousel, first box fastener and the mutually supporting lock joint of second box fastener, the downside middle part of transparent pressure strip seted up with knob assorted recess.

Preferably, protection machanism includes transparent protection casing and annular protection plate, the upper end fixed connection of annular protection plate and analysis appearance organism, and the annular protection plate cover is established in the outside of carousel, the outside at the annular protection plate is established to transparent protection casing cover, and the upper end right side rotation of hinge and analysis appearance organism is connected in one side of transparent protection casing.

Preferably, the surface of the knob is provided with anti-skid grains.

The utility model discloses a technological effect and advantage:

1. through the provided analyzer body, the speed regulation motor, the rotating shaft, the rotating disc, the rotating rod, the first bevel gear, the knob, a plurality of second bevel gears, a plurality of threaded rods, the mutual matching of a plurality of threaded cylinders and a plurality of cylinders, the rotating knob can drive the rotating rod to rotate, the rotating rod drives the first bevel gear to rotate, the first bevel gear drives the plurality of second bevel gears to rotate, the plurality of second bevel gears drive the plurality of threaded rods to rotate, the plurality of threaded rods can drive the plurality of threaded cylinders to move, the plurality of threaded cylinders can drive the plurality of cylinders to move, the plurality of cylinders can drive the plurality of centrifugal test tubes to move, the distance between the plurality of centrifugal test tubes and the center of the rotating disc can be conveniently adjusted, and therefore the centrifugal force applied to the centrifugal test tubes when the speed regulation motor drives the rotating disc to rotate is facilitated;

2. through the transparent pressing plate, after the centrifugal test tubes to be centrifuged are mounted, the transparent pressing plate is rotated to mutually lock the first box fastener and the second box fastener, so that a plurality of centrifugal test tubes can be pressed, and the phenomenon that the centrifugal test tubes shake to cause liquid to flow out when the centrifugal test tubes are rotated and centrifuged is avoided;

3. through mutually supporting of the annular guard plate that is equipped with and transparent protection casing, when needs rotate the centrifugation, overturn transparent protection casing, make the upper end of transparent protection casing and analysis appearance organism closed to can protect the top of analysis appearance organism, avoid people to miss to touch and receive the injury.

Drawings

Fig. 1 is a schematic front sectional structure of the present invention;

fig. 2 is a schematic top view of the turntable according to the present invention;

fig. 3 is an enlarged schematic structural view of a portion a in fig. 1 according to the present invention;

fig. 4 is an enlarged schematic structural diagram of a portion B in fig. 1 according to the present invention.

In the figure: 1. an analyzer body; 2. a fluorescence detection module; 3. a turntable; 4. a first bevel gear; 5. a knob; 6. a threaded rod; 7. a second bevel gear; 8. a threaded barrel; 9. a cylinder; 10. centrifuging the test tube; 11. a speed-regulating motor; 12. a rotating shaft; 13. a slider; 14. a transparent pressure strip; 15. a first box fastener; 16. a second box fastener; 17. a transparent protective cover; 18. and (4) an annular protection plate.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

The utility model provides a centrifugal PCR analyzer for SNP detection as shown in figures 1-4, which comprises an analyzer body 1, a circular groove is arranged at the middle part of the upper end of the analyzer body 1, a circular groove is arranged at the lower part of the circumferential side wall of the circular groove, a fluorescence detection module 2 is fixedly connected with the inside of the circular groove, a rotary disc 3 is rotatably connected with the upper middle part of the analyzer body 1 through a rotary mechanism, a circular cavity is arranged at the middle part of the inside of the rotary disc 3, the inside of the circular cavity is rotatably connected with a vertically arranged rotary rod through a first bearing, a first bevel gear 4 is fixedly sleeved at the lower part of the rod wall of the rotary rod, the upper end of the rotary rod extends to the upper part of the rotary disc 3 and is fixedly connected with a knob 5, a plurality of bar-shaped ports which are uniformly distributed are also arranged at the upper end of the rotary disc 3, a threaded rod 6 is rotatably connected with a second bearing at the inside of the plurality of the bar-shaped ports, one end of the plurality of the threaded rod 6 which is close to the circular cavity extends to the inside of the circular cavity and is fixedly connected with a second bevel gear 7, a plurality of second bevel gears 7 all are connected with the meshing of first bevel gear 4, and the equal screw thread of the pole wall of a plurality of threaded rods 6 has cup jointed threaded section of thick bamboo 8, and one side fixedly connected with drum 9 of threaded section of thick bamboo 8, the inside of drum 9 is inserted and is equipped with centrifugal test tube 10, and the top of carousel 3 is equipped with hold-down mechanism, and the top of analysis appearance organism 1 is equipped with protection machanism, and fluorescence detection module 2 can detect the inside liquid of centrifugal test tube 10, and this is prior art.

As shown in fig. 1, the rotary mechanism includes an adjustable speed motor 11, a motor cavity has been seted up to the inside below of analyzer organism 1, adjustable speed motor 11 is located the fixed setting in inside in motor cavity, and the upper end of adjustable speed motor 11 is rotated through the shaft coupling and is connected with pivot 12, the upper end of pivot 12 extend to the inside of circular slot and with the lower extreme middle part fixed connection of carousel 3, the axle wall of pivot 12 is connected through the last side wall rotation of third bearing with the motor cavity, it is rotatory that start-up adjustable speed motor 11 can drive pivot 12, pivot 12 can drive carousel 3 rotatoryly, thereby can drive a plurality of centrifugal test tubes 10 and rotate, can carry out centrifugal detection to the liquid in a plurality of centrifugal test tubes 10.

As shown in figure 2, one side of the cylinder 9, which is far away from the threaded rod 6, is fixedly connected with a sliding block 13, and a sliding groove matched with the sliding block 13 is formed in the side wall of the strip-shaped opening, so that the cylinder 9 is more stable when the strip-shaped opening moves.

As shown in fig. 1, fig. 3 and fig. 4, hold-down mechanism includes transparent pressure strip 14, the right side of transparent pressure strip 14 is passed through the hinge and is connected with the right side rotation of carousel 3, the left end fixedly connected with first box fastener 15 of transparent pressure strip 14, the left end fixedly connected with second box fastener 16 of carousel 3, first box fastener 15 and the mutual cooperation fastening of second box fastener 16, the downside middle part of transparent pressure strip 14 is seted up with knob 5 assorted recess, after treating centrifugal test tube 10 of centrifugation finishes the installation, rotate transparent pressure strip 14, with first box fastener 15 and the mutual fastening of second box fastener 16, thereby can compress tightly a plurality of centrifugal test tubes 10, centrifugal test tube 10 rocks and leads to the liquid outflow when avoiding rotating the centrifugation.

As shown in fig. 1, the protection mechanism comprises a transparent protection cover 17 and an annular protection plate 18, the annular protection plate 18 is fixedly connected with the upper end of the analyzer body 1, the annular protection plate 18 is sleeved on the outer portion of the rotary table 3, the transparent protection cover 17 is sleeved on the outer portion of the annular protection plate 18, one side of the transparent protection cover 17 is rotatably connected with the upper end right side of the analyzer body 1 through a hinge, when the centrifugal force is required to be rotated, the transparent protection cover 17 is turned over, the upper end of the transparent protection cover 17 and the upper end of the analyzer body 1 are closed, therefore, the upper portion of the analyzer body 1 can be protected, and people are prevented from being mistakenly touched and injured.

As shown in fig. 3, the surface of the knob 5 is provided with anti-slip lines, which is convenient for people to rotate the knob 5.

The utility model discloses the theory of operation:

when the device is used, the rotating knob 5 can be rotated to drive the rotating rod to rotate, the rotating rod drives the first bevel gear 4 to rotate, the first bevel gear 4 drives the second bevel gears 7 to rotate, the second bevel gears 7 drive the threaded rods 6 to rotate, the threaded rods 6 can drive the threaded cylinders 8 to move in a rotating mode, the threaded cylinders 8 can drive the cylinders 9 to move in a moving mode, the distance between the cylinders 9 and the center of the turntable 3 can be conveniently adjusted, and the centrifugal test tubes 10 filled with detection liquid are respectively inserted into the cylinders 9;

the transparent pressure strip 14 is turned over, the first box fastener 15 and the second box fastener 16 are buckled with each other, so that a plurality of centrifugal test tubes 10 can be pressed tightly, liquid flowing out caused by shaking of the centrifugal test tubes 10 during rotation and centrifugation is avoided, the transparent protective cover 17 is turned over, the transparent protective cover 17 is closed with the upper end of the analyzer body 1, the upper side of the analyzer body 1 can be protected, and people are prevented from being injured due to mistaken touch;

start speed regulating motor 11 and can drive pivot 12 rotatory, pivot 12 can drive 3 rotations of carousel to can drive a plurality of centrifugal test tubes 10 and rotate, can carry out the centrifugation to the liquid in a plurality of centrifugal test tubes 10, can detect the liquid after the centrifugation in a plurality of centrifugal test tubes 10 through fluorescence detection module 2.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.

Claims

1. A centrifugal PCR analyzer for SNP detection is characterized in that: comprises an analyzer body (1), a circular groove is formed in the middle of the upper end of the analyzer body (1), a circular groove is formed below the circumferential side wall of the circular groove, a fluorescence detection module (2) is fixedly connected inside the circular groove, a rotary disc (3) is rotatably connected in the middle of the upper portion of the analyzer body (1) through a rotating mechanism, a circular cavity is formed in the middle of the inner portion of the rotary disc (3), the inner portion of the circular cavity is rotatably connected with a vertically arranged rotary rod through a first bearing, a first bevel gear (4) is fixedly sleeved below the rod wall of the rotary rod, the upper end of the rotary rod extends to the upper portion of the rotary disc (3) and is fixedly connected with a knob (5), a plurality of uniformly distributed bar-shaped ports are further formed in the upper end of the rotary disc (3), and threaded rods (6) are rotatably connected inside the bar-shaped ports through second bearings, a plurality of the one end that is close to circular cavity of threaded rod (6) all extends to the inside of circular cavity and equal fixedly connected with second bevel gear (7), and is a plurality of second bevel gear (7) all are connected with first bevel gear (4) meshing, and are a plurality of the equal screw thread of pole wall of threaded rod (6) has cup jointed threaded section of thick bamboo (8), one side fixedly connected with drum (9) of threaded section of thick bamboo (8), the inside of drum (9) is inserted and is equipped with centrifugal test tube (10), the top of carousel (3) is equipped with hold-down mechanism, the top of analysis appearance organism (1) is equipped with protection machanism.

2. The centrifugal PCR analyzer for SNP detection according to claim 1, wherein: the rotary mechanism comprises an adjustable speed motor (11), a motor cavity is formed in the inner lower portion of the analyzer body (1), the adjustable speed motor (11) is located in the motor cavity and fixedly arranged in the inner portion, a rotating shaft (12) is connected to the upper end of the adjustable speed motor (11) in a rotating mode through a coupler, the upper end of the rotating shaft (12) extends to the inner portion of the circular groove and is fixedly connected with the middle of the lower end of the rotary table (3), and the shaft wall of the rotating shaft (12) is connected with the upper side wall of the motor cavity through a third bearing in a rotating mode.

3. The centrifugal PCR analyzer for SNP detection according to claim 1, wherein: one side fixedly connected with slider (13) that threaded rod (6) were kept away from in drum (9), the lateral wall of bar mouth is seted up with slider (13) assorted spout.

4. The centrifugal PCR analyzer for SNP detection according to claim 1, wherein: hold-down mechanism includes transparent pressure strip (14), the right side of transparent pressure strip (14) is passed through the hinge and is rotated with the right side of carousel (3) and be connected, the left end fixedly connected with first box fastener (15) of transparent pressure strip (14), the left end fixedly connected with second box fastener (16) of carousel (3), first box fastener (15) and second box fastener (16) mutually support the lock joint, the downside middle part of transparent pressure strip (14) is seted up with knob (5) assorted recess.

5. The centrifugal PCR analyzer for SNP detection according to claim 1, wherein: protection machanism includes transparent protection casing (17) and annular protection board (18), the upper end fixed connection of annular protection board (18) and analysis appearance organism (1), and annular protection board (18) cover is established in the outside of carousel (3), the outside at annular protection board (18) is established in transparent protection casing (17) cover, and the upper end right side rotation of hinge and analysis appearance organism (1) is connected in one side of transparent protection casing (17).

6. The centrifugal PCR analyzer for SNP detection according to claim 1, wherein: the surface of the knob (5) is provided with anti-skid grains.