CN213772068U - Saving type and low-damage tissue primary cell separation device - Google Patents
Saving type and low-damage tissue primary cell separation device Download PDFInfo
- Publication number
- CN213772068U CN213772068U CN202022627626.9U CN202022627626U CN213772068U CN 213772068 U CN213772068 U CN 213772068U CN 202022627626 U CN202022627626 U CN 202022627626U CN 213772068 U CN213772068 U CN 213772068U
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- China
- Prior art keywords
- fixed
- enzyme liquid
- cell
- liquid container
- filter screen
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- Expired - Fee Related
Links
- 238000000926 separation method Methods 0.000 title claims abstract description 20
- 239000007788 liquid Substances 0.000 claims abstract description 56
- 108090000790 Enzymes Proteins 0.000 claims abstract description 55
- 102000004190 Enzymes Human genes 0.000 claims abstract description 55
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000011084 recovery Methods 0.000 claims abstract description 13
- 238000009434 installation Methods 0.000 claims abstract description 11
- 239000000463 material Substances 0.000 claims description 33
- 238000007789 sealing Methods 0.000 claims description 11
- 238000007599 discharging Methods 0.000 claims description 8
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 7
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 7
- 241001330002 Bambuseae Species 0.000 claims description 7
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 7
- 239000011425 bamboo Substances 0.000 claims description 7
- 125000003003 spiro group Chemical group 0.000 claims description 6
- 230000006978 adaptation Effects 0.000 claims 1
- 238000002955 isolation Methods 0.000 claims 1
- 229940088598 enzyme Drugs 0.000 abstract description 41
- 108010019160 Pancreatin Proteins 0.000 abstract description 10
- 229940055695 pancreatin Drugs 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 3
- 210000002966 serum Anatomy 0.000 abstract description 3
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 64
- 239000012530 fluid Substances 0.000 description 9
- 238000003756 stirring Methods 0.000 description 7
- 238000001914 filtration Methods 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003701 histiocyte Anatomy 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 239000006193 liquid solution Substances 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Images
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a saving type, low damage tissue primary cell separation device in the technical field of tissue primary cell separation, the outer wall of the installation cylinder is uniformly and fixedly provided with a passive grinding rod, the inner cavity side wall of an enzyme liquid container is fixed with a first filter screen, the inner cavity bottom of the enzyme liquid container is fixed with a second filter screen, the top of the second filter screen is fixed with a magnetic stirrer, the right side wall of the enzyme liquid container is fixed with a water pump, the output end of the water pump is fixedly provided with an enzyme liquid recovery tube, the bottom outer wall of the guide tube is screwed with a cell collector, the bottom of the inner cavity side wall of the cell collector is fixedly provided with a cell collection filter screen, the separation of cell tissue blocks and pancreatin is realized, cells are separated from pancreatin contact and are protected by serum, the damage of the cell tissue is reduced compared with the traditional method, and the cell activity is enhanced, avoid damaging cells as much as possible, contribute to enhancing cell activity and improving cell yield.
Description
Technical Field
The utility model relates to a primary cell separation technical field of tissue specifically is a primary cell separator of tissue of saving type, low damage.
Background
The cell culture is a culture technology for taking cells out of tissues in vivo to mimic the environment appearing in vivo, growing and propagating the cells in the body and maintaining the structure and functions of the cells under the conditions of sterility, proper temperature, proper pH value and certain nutrition.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a primary cell separator of tissue of saving type, low damage to solve the problem that proposes in the above-mentioned background art.
In order to achieve the above object, the utility model provides a following technical scheme: a saving type low-damage tissue primary cell separation device comprises an enzyme liquid container, wherein supporting legs are fixed at four corners of the bottom of the enzyme liquid container, a stirring motor is fixed at the top of the enzyme liquid container, an output end of the stirring motor extends into the enzyme liquid container and is fixedly assembled with a mounting cylinder, a passive grinding rod is uniformly and fixedly assembled on the outer wall of the mounting cylinder, a feed hopper is fixedly assembled on the right side of the top of the enzyme liquid container, a first filter screen is fixed on the side wall of an inner cavity of the enzyme liquid container, a second filter screen is fixed at the bottom of the inner cavity of the enzyme liquid container, a material guide channel matched with the second filter screen is arranged at the center of the bottom of the enzyme liquid container, a magnetic stirrer is fixed at the top of the second filter screen, a water pump is fixed on the right side wall of the enzyme liquid container, an enzyme liquid recovery pipe is fixed at the output end of the water pump, a material guide hopper is fixed at the bottom of the enzyme liquid container, the fixed passage that is equipped with in bottom of baffle box, the bottom outer wall spiro union of passage is equipped with the cell collector, the fixed cell collection filter screen that is equipped with in cell collector inner chamber lateral wall bottom, the bottom spiro union of cell collector is equipped with row material connecting hopper, it is equipped with the sealing washer to arrange the fixed bottom of material connecting hopper, arrange the fixed row's of being equipped with in bottom of material connecting hopper material pipe, just arrange material pipe and enzyme liquid recovery tube intercommunication.
Preferably, the outer wall of the mounting cylinder is provided with a threaded hole, and the blunt grinding rod is integrally provided with a screw rod matched with the threaded hole.
Preferably, blunt grinding rod evenly sets up along the length direction of installation section of thick bamboo, just blunt grinding rod is the grinding rod that length reduces in proper order.
Preferably, the cell collector is screwed on the outer wall of the guide tube, and the bottom end of the guide tube is flush with the top of the inner cavity of the cell collector.
Preferably, the bottom of the cell collector is provided with a mounting hole matched with the discharging connecting hopper, the sealing ring is fixedly assembled at the top in the mounting hole, and the size of the sealing ring is matched with that of the top of the discharging connecting hopper.
Preferably, arrange the material pipe and the enzyme liquid recovery pipe intercommunication, first filter screen and installation section of thick bamboo bottom correspond, just the water pump is fixed at the right side wall top of enzyme liquid container.
Compared with the prior art, the beneficial effects of the utility model are that: the utility model has the advantages of reasonable design, realize the leading-in of cell tissue liquid through the feeder hopper, agitator motor drives the rotation of the blunt grinding rod on the installation section of thick bamboo and can stir the cell tissue liquid in the enzyme liquid solution and grind, can accelerate cell separation, the enzyme liquid of separation completion can be layered above simultaneously, the water pump is so derived the enzyme liquid through enzyme liquid recovery tube, realize the separation to cell tissue piece and pancreatin, the cell breaks away from the pancreatin contact and obtains the protection of serum, the damage of cell tissue reduces than traditional method, cell vitality is strengthened, avoid damaging the cell as far as possible, help strengthening cell vitality, cell yield is improved, first filter screen can filter the cell tissue piece that gets off of separation, then magnetic stirrers can shake to cell tissue liquid and break up the separation, realize the filtration to the tissue liquid through the second filter screen, the cell that the filtration was accomplished is derived through the guide passageway after filtering out through the second filter screen, the material guide hopper is convenient for realizing blanking of histiocytes and can rapidly enter the cell collector through the material guide pipe, and pancreatin liquid attached to the cell tissues is filtered by the cell collection filter screen and then is guided out through the material discharge pipe to realize collection and be convenient for recycling.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
fig. 2 is the schematic view of the cell collector and the discharging connecting hopper assembly structure of the present invention.
In the figure: 1. an enzyme solution container; 2. a support leg; 3. a stirring motor; 4. mounting the cylinder; 5. a passive grinding rod; 6. a feed hopper; 7. a first filter screen; 8. a second filter screen; 9. a magnetic stirrer; 10. a material guide channel; 11. a water pump; 12. an enzyme solution recovery pipe; 13. a material guide hopper; 14. a material guide pipe; 15. a cell collector; 16. a cell collection filter screen; 17. a discharge connecting hopper; 18. a seal ring; 19. a discharge pipe.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1 and fig. 2, the present invention provides a technical solution: a saving type low-damage tissue primary cell separation device comprises an enzyme liquid container 1, supporting legs 2 are fixed at four corners of the bottom of the enzyme liquid container 1, a stirring motor 3 is fixed at the top of the enzyme liquid container 1, an installation cylinder 4 is fixedly assembled after the output end of the stirring motor 3 extends into the enzyme liquid container 1, blunt grinding rods 5 are uniformly and fixedly assembled on the outer wall of the installation cylinder 4, a feed hopper 6 is fixedly assembled at the right side of the top of the enzyme liquid container 1, a first filter screen 7 is fixed on the side wall of an inner cavity of the enzyme liquid container 1, a second filter screen 8 is fixed at the bottom of the inner cavity of the enzyme liquid container 1, a material guide channel 10 matched with the second filter screen 8 is arranged at the center of the bottom of the enzyme liquid container 1, a magnetic stirrer 9 is fixed at the top of the second filter screen 8, a water pump 11 is fixed on the right side wall of the enzyme liquid container 1, an enzyme liquid recovery pipe 12 is fixedly assembled at the output end of the water pump 11, the bottom of enzyme liquid container 1 is fixed with baffle box 13, the bottom of baffle box 13 is fixed with baffle tube 14, the bottom outer wall spiro union of baffle tube 14 is equipped with cell collector 15, cell collector 15 inner chamber lateral wall bottom is fixed with cell collection filter screen 16, the bottom spiro union of cell collector 15 is equipped with row material connecting bucket 17, row material connecting bucket 17 top is fixed with sealing washer 18, row material connecting bucket 17's bottom is fixed with row material pipe 19, and row material pipe 19 and enzyme liquid recovery tube 12 intercommunication.
The outer wall of the mounting cylinder 4 is provided with a threaded hole, and the blunt grinding rod 5 is integrally provided with a threaded rod matched with the threaded hole, so that the installation of the blunt grinding rod 5 is convenient and quick;
referring to fig. 1, the inactive grinding rods 5 are uniformly arranged along the length direction of the mounting cylinder 4, and the inactive grinding rods 5 are grinding rods with sequentially decreasing lengths, so that the cell tissue fluid can be speeded up to be separated when being stirred by the inactive grinding rods 5;
the cell collector 15 is screwed and assembled on the outer wall of the material guide pipe 14, and the bottom end of the material guide pipe 14 is flush with the top of the inner cavity of the cell collector 15;
the bottom of the cell collector 15 is provided with a mounting hole matched with the discharging connecting hopper 17, the sealing ring 18 is fixedly assembled at the top in the mounting hole, the size of the sealing ring 18 is matched with that of the top of the discharging connecting hopper 17, the sealing performance is improved through the sealing ring 18, and meanwhile, the cell collector 15 can be rapidly taken down after the cell collector 15 finishes cell collection, and the collected cells can be conveniently taken out at any time;
the discharge pipe 19 is communicated with the enzyme liquid recovery pipe 12, the first filter screen 7 corresponds to the bottom of the mounting cylinder 4, and the water pump 11 is fixed on the top of the right side wall of the enzyme liquid container 1.
The working principle is as follows: the introduction of cell tissue fluid is realized through a feed hopper 6, a stirring motor 3 drives a passive grinding rod 5 on a mounting cylinder 4 to rotate, the cell tissue fluid in the enzyme fluid solution can be stirred and ground, the cell separation can be accelerated, and simultaneously, the separated pancreatin fluid can be layered above, so that the enzyme fluid is led out through an enzyme fluid recovery pipe 12 by a water pump 11, the separation of cell tissue blocks and pancreatin is realized, the cells are separated from the contact of the pancreatin and are protected by serum, the damage of the cell tissue is reduced compared with the traditional method, the cell activity is enhanced, the damage to the cells is avoided as much as possible, the cell activity is enhanced, the cell yield is improved, the separated cell tissue blocks can be filtered by a first filter screen 7, then a magnetic stirrer 9 can vibrate, the cell tissue fluid is scattered and separated, the tissue fluid is filtered by a second filter screen 8, the filtered cells are led out through a material guide channel 10 after being filtered by the second filter screen 8, the material guide hopper 13 is convenient for discharging tissue cells, can quickly enter the cell collector 15 through the material guide pipe 14, and pancreatin liquid attached to cell tissues is filtered by the cell collection filter screen 16 and then is led out through the material discharge pipe 19 for collection, so that the pancreatin liquid can be recycled.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A saving type low-damage tissue primary cell separation device comprises an enzyme liquid container (1), wherein supporting legs (2) are fixed at four corners of the bottom of the enzyme liquid container (1), and the device is characterized in that: the top of enzyme liquid container (1) is fixed with agitator motor (3), the output of agitator motor (3) extends into enzyme liquid container (1) after the fixed installation section of thick bamboo (4) that is equipped with, even fixed blunt grinding rod (5) of being equipped with on the outer wall of installation section of thick bamboo (4), the fixed feeder hopper (6) that is equipped with in top right side of enzyme liquid container (1), be fixed with first filter screen (7) on the inner chamber lateral wall of enzyme liquid container (1), the inner chamber bottom of enzyme liquid container (1) is fixed with second filter screen (8), the bottom center department of enzyme liquid container (1) sets up guide passageway (10) with second filter screen (8) looks adaptation, the top of second filter screen (8) is fixed with magnetic stirrer (9), be fixed with water pump (11) on the right side wall of enzyme liquid container (1), the fixed enzyme liquid recovery tube (12) that is equipped with in output of water pump (11), the bottom of enzyme liquid container (1) is fixed with baffle box (13), the fixed passage (14) that is equipped with in bottom of baffle box (13), the bottom outer wall spiro union of passage (14) is equipped with cell collector (15), cell collector (15) inner chamber lateral wall bottom fixed be equipped with cell collection filter screen (16), the bottom spiro union of cell collector (15) is equipped with row material connecting hopper (17), row material connecting hopper (17) top fixed be equipped with sealing washer (18), the fixed row of being equipped with in bottom of arranging material connecting hopper (17) expects pipe (19), just arrange material pipe (19) and enzyme liquid recovery tube (12) intercommunication.
2. The economical, low-damage tissue primary cell separation device according to claim 1, characterized in that: the outer wall of the mounting cylinder (4) is provided with a threaded hole, and the blunt grinding rod (5) is integrally provided with a screw rod matched with the threaded hole.
3. A conservation-oriented, low-damage tissue primary cell isolation apparatus as claimed in claim 2, wherein: blunt grinding rod (5) evenly set up along the length direction of installation section of thick bamboo (4), just blunt grinding rod (5) are the grinding rod that length reduces in proper order.
4. The economical, low-damage tissue primary cell separation device according to claim 1, characterized in that: the cell collector (15) is assembled on the outer wall of the material guide pipe (14) in a threaded manner, and the bottom end of the material guide pipe (14) is flush with the top of the inner cavity of the cell collector (15).
5. The economical, low-damage tissue primary cell separation device according to claim 1, characterized in that: the bottom of the cell collector (15) is provided with a mounting hole matched with the discharging connecting hopper (17), the sealing ring (18) is fixedly assembled at the top in the mounting hole, and the size of the sealing ring (18) is matched with the size of the top of the discharging connecting hopper (17).
6. The economical, low-damage tissue primary cell separation device according to claim 1, characterized in that: arrange material pipe (19) and enzyme liquid recovery tube (12) intercommunication, first filter screen (7) and installation section of thick bamboo (4) bottom are corresponding, just water pump (11) are fixed in the right side wall top of enzyme liquid container (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022627626.9U CN213772068U (en) | 2020-11-13 | 2020-11-13 | Saving type and low-damage tissue primary cell separation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022627626.9U CN213772068U (en) | 2020-11-13 | 2020-11-13 | Saving type and low-damage tissue primary cell separation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213772068U true CN213772068U (en) | 2021-07-23 |
Family
ID=76916306
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022627626.9U Expired - Fee Related CN213772068U (en) | 2020-11-13 | 2020-11-13 | Saving type and low-damage tissue primary cell separation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213772068U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114112611A (en) * | 2021-10-08 | 2022-03-01 | 重庆金域医学检验所有限公司 | Washing unit is collected to cell pathology section |
-
2020
- 2020-11-13 CN CN202022627626.9U patent/CN213772068U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114112611A (en) * | 2021-10-08 | 2022-03-01 | 重庆金域医学检验所有限公司 | Washing unit is collected to cell pathology section |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210723 |