CN213739497U

CN213739497U - Auxiliary amplification device for high-throughput sequencing library construction

Info

Publication number: CN213739497U
Application number: CN202022622994.4U
Authority: CN
Inventors: 朱杰; 陈青云
Original assignee: Wuhan Hsa Bind Biotechnology Co ltd
Current assignee: Wuhan Hsa Bind Biotechnology Co ltd
Priority date: 2020-11-13
Filing date: 2020-11-13
Publication date: 2021-07-20
Anticipated expiration: 2030-11-13

Abstract

The utility model discloses a supplementary amplification device of high throughput sequencing literary composition founds, including fixed storehouse, fixed storehouse bottom inner wall bonds there is the heating frame, and the inside heating wire that is equipped with of heating frame, fixed storehouse inner wall bonds in the top department of heating frame has first heat-conducting plate, first heat-conducting plate top both sides all bond has fixed pipe, fixed intraductal wall bonds has the spring strip, the spring strip top bonds there is the movable block, the movable block top bonds there is the push rod, fixed storehouse top inner wall bonds there is the second heat-conducting plate, is equipped with the distilled water between first heat-conducting plate and the second heat-conducting plate, the push rod bonds with the second heat-conducting plate, and fixed storehouse one side bonds there is the power control case, the inside intermediate layer that is equipped with in fixed storehouse, fixed storehouse department bonds there is the rock wool layer in the intermediate layer, it has the rubber ring to bond between second heat-conducting plate and the fixed storehouse. The utility model discloses a heating distilled water makes the distilled water vaporization become high temperature steam to it is more even to make the device heating alternating temperature.

Description

Auxiliary amplification device for high-throughput sequencing library construction

Technical Field

The utility model relates to a gene amplification technical field especially relates to a supplementary amplification device of high throughput sequencing library construction.

Background

In the process of constructing a high-throughput sequencing library, a large amount of gene data is needed, so that the gene needs to be amplified, different constant temperature needs to be ensured during gene amplification, different equipment is needed when constant temperature heating devices with different temperatures cannot adjust the constant temperature heating time, and the efficiency is low.

Through the retrieval, chinese patent publication is CN 207998614U's patent discloses a high flux preface literary composition storehouse founds amplification device, including heating cabinet (1), snakelike heat pipe (2), hot feed liquor pipe (201), hot drain pipe (202), heating cylinder (3), heating resistor (4), circulating pump (5), microcontroller (6), touch-sensitive screen (7), the utility model discloses a circulating pump and heating power dual regulation can control the temperature of constant temperature heating effectively, can carry out different constant temperature heating temperature's settlement, and the while heats evenly, and the alternating temperature is rapid.

The above patent uses the snakelike heat pipe to heat the heating box, but because the snakelike heat pipe can not fill up whole heating box, so the heating box top still can have inhomogeneous by being heated, leads to the alternating temperature slowly.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects existing in the prior art and providing a high-throughput sequencing library construction auxiliary amplification device.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides a supplementary amplification device of high flux sequencing library structure, includes fixed storehouse, fixed storehouse bottom inner wall bonds has the heating frame, and the inside heating wire that is equipped with of heating frame, fixed storehouse inner wall bonds in the top department of heating frame has first heat-conducting plate, first heat-conducting plate top both sides all bond has fixed pipe, fixed pipe inner wall bonds has the spring strip, the spring strip top bonds has the movable block, the movable block top bonds has the push rod, fixed storehouse top inner wall bonds has the second heat-conducting plate, be equipped with the distilled water between first heat-conducting plate and the second heat-conducting plate, the push rod bonds with the second heat-conducting plate, and fixed storehouse one side bonds has power control box, the inside intermediate layer that is equipped with of fixed storehouse, and fixed storehouse bonds at the intermediate layer has the rock wool layer, it has the rubber ring to bond between second heat-conducting plate and the fixed storehouse.

Furthermore, the first heat-conducting plate and the second heat-conducting plate are both made of copper materials.

Furthermore, a power module and a control module are arranged in the power control box, and the power control box is electrically connected with the heating wire.

Furthermore, one side of the fixed bin is rotatably connected with a rotating plate through a rotating shaft, and one end of the rotating plate is bonded with a baffle.

Furthermore, rock wool pads are bonded on one side of the baffle, and handle rings are bonded on two ends of one side of the baffle.

Furthermore, a fixing block is bonded on one side of the fixing bin, and a clamping rod is connected to one side of the fixing block in a sliding mode.

Further, fixed storehouse one side is equipped with the fixed disk, and the fixed disk both sides all bond there is the screw thread piece, the fixed disk passes through the bolt bonding with external mechanism, a plurality of card hole has been seted up to fixed disk one side.

The utility model has the advantages that:

1. through setting up first heat-conducting plate, the second heat-conducting plate, the heating frame, the heating wire, spring strip and push rod, when needing opening device to heat, when heating frame heating at first, first heat-conducting plate heating, heat transfer is for the distilled water simultaneously, make inside water vaporization become high temperature steam, when distilled water constantly vaporizes simultaneously, the internal pressure increases, it rises to drive first heat-conducting plate, the spring strip is tensile, vapor is filled between first heat-conducting plate and second heat-conducting plate, evenly carry out heat transfer to the second heat-conducting plate, it is more even to make the second heat-conducting plate be heated, the alternating temperature efficiency has been improved.

2. Through setting up the baffle, rotor plate and rock wool pad, when need not heat, rotate the rotor plate, the rotor plate drives the baffle and blocks the second heat-conducting plate automatically, when avoiding scalding the staff, has also avoided when not being suitable for the device to heat, and debris that fall on the first heat-conducting plate lead to, and it is inhomogeneous to be heated, has protected the device.

3. Through setting up the screw thread piece, card hole and kelly, when needs adjusting device's height, at first the pulling kelly to thereby the height of fixed storehouse is adjusted to the slip fixed block, makes the device can carry out altitude mixture control according to the external mechanism of different grade type, has improved the universality.

Drawings

FIG. 1 is a front view of an apparatus for constructing an auxiliary amplification device by using a high throughput sequencing library as set forth in example 1;

FIG. 2 is a front sectional view of an amplification device with the aid of high throughput sequencing library construction as set forth in example 1;

FIG. 3 is a front view of a rubber ring for constructing an auxiliary amplification device by using a high throughput sequencing library as set forth in example 1;

FIG. 4 is a front view of the fixed block of a high throughput sequencing library-based amplification device as set forth in example 2.

In the figure: 1-fixed bin, 2-power control box, 3-rock wool layer, 4-rubber ring, 5-second heat conducting plate, 6-interlayer, 7-fixed tube, 8-push rod, 9-moving block, 10-spring strip, 11-first heat conducting plate, 12-heating frame, 13-heating wire, 14-bracelet, 15-baffle, 16-rock wool pad, 17-rotating plate, 18-fixed disk, 19-fixed block, 20-clamping rod, 21-clamping hole and 22-thread block.

Detailed Description

The technical solution of the present invention will be described in further detail with reference to the following embodiments.

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present invention, and should not be construed as limiting the present invention.

In the description of the present invention, it is to be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are merely for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, are not to be construed as limiting the present invention.

In the description of the present invention, it should be noted that unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "disposed" are to be construed broadly and can include, for example, a fixed connection, a removable connection, or an integral connection or a removable arrangement. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

Example 1

Referring to fig. 1-3, a high-throughput sequencing library construction auxiliary amplification device comprises a fixed bin 1, wherein a heating frame 12 is bonded to the inner wall of the bottom end of the fixed bin 1, a heating wire 13 is arranged inside the heating frame 12, a first heat conduction plate 11 is bonded to the inner wall of the fixed bin 1 above the heating frame 12, fixed tubes 7 are bonded to both sides of the top end of the first heat conduction plate 11, spring strips 10 are bonded to the inner wall of the fixed tubes 7, movable blocks 9 are bonded to the top ends of the spring strips 10, push rods 8 are bonded to the top ends of the movable blocks 9, a second heat conduction plate 5 is bonded to the inner wall of the top end of the fixed bin 1, distilled water is arranged between the first heat conduction plate 11 and the second heat conduction plate 5, the push rods 8 are bonded to the second heat conduction plate 5, a power control box 2 is bonded to one side of the fixed bin 1, an interlayer 6 is arranged inside the fixed bin 1, a rock wool layer 3 is bonded to the interlayer 6 of the fixed bin 1, the rubber ring 4 is bonded between the second heat-conducting plate 5 and the fixed bin 1, when the opening device is required to heat, firstly, when the heating frame 12 is heated, the first heat-conducting plate 11 is heated, meanwhile, heat is transferred to distilled water, so that the internal water is vaporized into high-temperature steam, meanwhile, when the distilled water is constantly vaporized, the internal pressure is increased, the first heat-conducting plate 11 is driven to ascend, the spring strip 10 is stretched, the water vapor is filled between the first heat-conducting plate 11 and the second heat-conducting plate 5, the heat is uniformly transferred to the second heat-conducting plate 5, so that the second heat-conducting plate 5 is heated more uniformly, and the temperature change efficiency is improved.

Wherein, first heat-conducting

plate

11 and 5 materials of second heat-conducting plate are the copper product, 2 inside power module and the control module of being equipped with of power control box,

power control box

2 and 13 electric connections of heating wire, 1 one side of fixed bin is rotated through the pivot and is connected with rotor plate 17, rotor plate 17 one end bonds there is

baffle

15, 15 one side of baffle bonds there is the

rock wool pad

16, 15 one side both ends of baffle bond there is handle ring 14, when need not heat, rotate rotor plate 17, rotor plate 17 drives baffle 15 and blocks second heat-conducting plate 5 automatically, when avoiding scalding the staff, has also avoided when not being suitable for the device to heat, and the debris that falls on first heat-conducting plate 11 leads to, and it is inhomogeneous to be heated, has protected the device.

The working principle is as follows: when the opening device is needed to heat, when the heating frame 12 is heated at first, the first heat conduction plate 11 is heated, and meanwhile, heat is transferred to distilled water, so that the internal water is vaporized into high-temperature steam, and meanwhile, when the distilled water is continuously vaporized, the internal pressure is increased to drive the first heat conduction plate 11 to ascend, the spring strip 10 is stretched, the steam is filled between the first heat conduction plate 11 and the second heat conduction plate 5, so that the heat is transferred to the second heat conduction plate 5 uniformly, the second heat conduction plate 5 is heated more uniformly, and the temperature change efficiency is improved.

When not needing to heat, rotate rotor plate 17, rotor plate 17 drives baffle 15 and blocks second heat-conducting plate 5 automatically, when avoiding scalding the staff, has also avoided when not being suitable for the device to heat, and debris that falls on first heat-conducting plate 11 lead to, and it is inhomogeneous to be heated, has protected the device.

Example 2

Referring to fig. 4, a supplementary amplification device of high throughput sequencing library structure, this embodiment compares in embodiment 1, in order to improve the universality of device, fixed 1 one side of storehouse bonds and has fixed block 19, and fixed 19 one side sliding connection has kelly 20, fixed 1 one side of storehouse is equipped with fixed disk 18, and fixed disk 18 both sides all bond and have screw thread piece 22, fixed disk 18 passes through the bolt bonding with external mechanism, a plurality of card hole 21 has been seted up to fixed disk 18 one side, when needs adjusting device's height, at first stimulates kelly 20 to thereby slide fixed block 19 adjusts the height of fixed storehouse 1, makes the device can carry out altitude mixture control according to the external mechanism of different grade type, has improved the adaptability.

When the height of the device needs to be adjusted, the clamping rod 20 is pulled at first, and the fixing block 19 is slid, so that the height of the fixing bin 1 is adjusted, the device can be adjusted in height according to different types of external mechanisms, and the universality is improved.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. A high-throughput sequencing library construction auxiliary amplification device comprises a fixed bin (1) and is characterized in that a heating frame (12) is fixedly connected to the inner wall of the bottom end of the fixed bin (1), an electric heating wire (13) is arranged inside the heating frame (12), a first heat-conducting plate (11) is fixedly connected to the inner wall of the fixed bin (1) above the heating frame (12), fixed tubes (7) are fixedly connected to two sides of the top end of the first heat-conducting plate (11), spring strips (10) are fixedly connected to the inner wall of the fixed tubes (7), movable blocks (9) are fixedly connected to the top ends of the spring strips (10), push rods (8) are fixedly connected to the top ends of the movable blocks (9), a second heat-conducting plate (5) is fixedly connected to the inner wall of the top end of the fixed bin (1), and distilled water is arranged between the first heat-conducting plate (11) and the second heat-conducting plate (5), push rod (8) and second heat-conducting plate (5) fixed connection, fixed storehouse (1) one side fixedly connected with power control box (2), fixed storehouse (1) inside is equipped with intermediate layer (6), and fixed storehouse (1) is in intermediate layer (6) fixedly connected with rock wool layer (3), fixedly connected with rubber ring (4) between second heat-conducting plate (5) and fixed storehouse (1).

2. The high-throughput sequencing library-building assisted amplification apparatus of claim 1, wherein the first heat-conducting plate (11) and the second heat-conducting plate (5) are both made of copper.

3. The high-throughput sequencing library construction assisted amplification device according to claim 2, wherein a power supply module and a control module are arranged inside the power supply control box (2), and the power supply control box (2) is electrically connected with the heating wire (13).

4. The high-throughput sequencing library construction-assisted amplification device according to claim 3, wherein a rotating plate (17) is rotatably connected to one side of the fixed bin (1) through a rotating shaft, and a baffle (15) is fixedly connected to one end of the rotating plate (17).

5. The high-throughput sequencing library construction auxiliary amplification device is characterized in that a rock wool pad (16) is fixedly connected to one side of the baffle plate (15), and a handle ring (14) is fixedly connected to two ends of one side of the baffle plate (15).

6. The high-throughput sequencing library construction-assisted amplification device according to claim 5, wherein a fixing block (19) is fixedly connected to one side of the fixing bin (1), and a clamping rod (20) is slidably connected to one side of the fixing block (19).

7. The high-throughput sequencing library construction auxiliary amplification device according to claim 6, wherein a fixed disc (18) is arranged on one side of the fixed bin (1), a thread block (22) is fixedly connected to each of two sides of the fixed disc (18), the fixed disc (18) is fixedly connected with an external mechanism through a bolt, and a plurality of clamping holes (21) are formed in one side of the fixed disc (18).