CN213739254U - Fixing device for protein purification - Google Patents
Fixing device for protein purification Download PDFInfo
- Publication number
- CN213739254U CN213739254U CN202022666027.8U CN202022666027U CN213739254U CN 213739254 U CN213739254 U CN 213739254U CN 202022666027 U CN202022666027 U CN 202022666027U CN 213739254 U CN213739254 U CN 213739254U
- Authority
- CN
- China
- Prior art keywords
- purification
- fixing plate
- placing
- protein purification
- seal box
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000001742 protein purification Methods 0.000 title claims abstract description 22
- 238000000746 purification Methods 0.000 claims abstract description 52
- 238000001816 cooling Methods 0.000 claims abstract description 13
- 238000007789 sealing Methods 0.000 claims abstract description 12
- 238000003860 storage Methods 0.000 claims description 8
- 239000002519 antifouling agent Substances 0.000 claims description 3
- 238000003466 welding Methods 0.000 claims description 3
- 230000008859 change Effects 0.000 claims description 2
- 230000005389 magnetism Effects 0.000 claims 2
- 102000004169 proteins and genes Human genes 0.000 description 9
- 108090000623 proteins and genes Proteins 0.000 description 9
- 230000000694 effects Effects 0.000 description 6
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 4
- 239000011347 resin Substances 0.000 description 4
- 229920005989 resin Polymers 0.000 description 4
- 239000002245 particle Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 238000010828 elution Methods 0.000 description 2
- 229920001821 foam rubber Polymers 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 238000011897 real-time detection Methods 0.000 description 2
- HBGPNLPABVUVKZ-POTXQNELSA-N (1r,3as,4s,5ar,5br,7r,7ar,11ar,11br,13as,13br)-4,7-dihydroxy-3a,5a,5b,8,8,11a-hexamethyl-1-prop-1-en-2-yl-2,3,4,5,6,7,7a,10,11,11b,12,13,13a,13b-tetradecahydro-1h-cyclopenta[a]chrysen-9-one Chemical compound C([C@@]12C)CC(=O)C(C)(C)[C@@H]1[C@H](O)C[C@]([C@]1(C)C[C@@H]3O)(C)[C@@H]2CC[C@H]1[C@@H]1[C@]3(C)CC[C@H]1C(=C)C HBGPNLPABVUVKZ-POTXQNELSA-N 0.000 description 1
- PFRGGOIBYLYVKM-UHFFFAOYSA-N 15alpha-hydroxylup-20(29)-en-3-one Natural products CC(=C)C1CCC2(C)CC(O)C3(C)C(CCC4C5(C)CCC(=O)C(C)(C)C5CCC34C)C12 PFRGGOIBYLYVKM-UHFFFAOYSA-N 0.000 description 1
- 229940124158 Protease/peptidase inhibitor Drugs 0.000 description 1
- SOKRNBGSNZXYIO-UHFFFAOYSA-N Resinone Natural products CC(=C)C1CCC2(C)C(O)CC3(C)C(CCC4C5(C)CCC(=O)C(C)(C)C5CCC34C)C12 SOKRNBGSNZXYIO-UHFFFAOYSA-N 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000003223 protective agent Substances 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Images
Landscapes
- Peptides Or Proteins (AREA)
Abstract
The utility model discloses a fixing device for protein purification, which comprises a base, a sealing box and a test tube placing cabinet, wherein the sealing box and the test tube placing cabinet are arranged on the base; an auxiliary fixing plate and a purification column fixing plate are horizontally arranged in the seal box from bottom to top respectively, the auxiliary fixing plate is provided with a plurality of placing holes for placing centrifuge tubes, the purification column fixing plate is provided with a plurality of placing holes for placing purification columns, and the placing holes of the auxiliary fixing plate and the purification column fixing plate are corresponding to each other in the vertical direction; a cooling device is arranged between the auxiliary fixing plate and the purification column fixing plate on the inner side wall of the seal box; the test tube is equipped with in placing the cabinet and places the board, places the board and is used for placing the centrifuging tube after the purification is accomplished. This kind of fixing device for protein purification can realize the stable fixed to purification post and centrifuging tube to can provide low temperature environment in the purification process, improve the purification result.
Description
Technical Field
The utility model belongs to the technical field of biological preparation equipment, in particular to fixing device is used in protein purification.
Background
The purification of the protein is roughly divided into two stages, namely a coarse separation stage and a fine purification stage, wherein the coarse separation stage mainly separates the target protein from other cell components such as DNA, RNA and the like, and the sample has large volume and mixed components, so that the resin used has the requirements of high capacity, high flow rate, large particles and wide particle size distribution, and in order to quickly separate the protein from pollutants, a corresponding protective agent (such as a protease inhibitor) can be added if necessary to prevent the target protein from being degraded; the fine purification stage needs higher resolution, and the stage is to distinguish the target protein from those with molecular weight and physical and chemical properties close to each other, to use smaller resin particles to improve the resolution, and to use ion exchange columns and hydrophobic columns, which are used in combination with two factors of resin selectivity and column efficiency. The selectivity refers to the specificity of the combination of the resin and the target protein, the column effect refers to the capability of intensively eluting each protein component from the resin one by one, and the narrower the elution peak, the better the column effect. Only good selectivity, too broad elution peak, and inefficient protein separation as it is.
The protein purification rack manufactured by the traditional iron stand platform can not control a plurality of gravity columns at the same time; the fixing device self-made by the centrifuge tube rack is very easy to collapse, thereby influencing the purification result; and the protein purification process is required to be carried out at a low temperature as far as possible, the time required by the purification process is long, and the traditional iron stand table or a self-made fixing device cannot keep the low temperature state at room temperature.
Based on the above analysis, the present inventors have made studies and improvements on the conventional immobilization device for protein purification, and have come to the present invention.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a fixing device is used in protein purification, it can realize the stable fixed of purification post and centrifuging tube to can provide low temperature environment in purification process, improve the purification result.
In order to achieve the above purpose, the solution of the present invention is:
a fixing device for protein purification comprises a base, a sealing box and a test tube placing cabinet, wherein the sealing box and the test tube placing cabinet are arranged on the base;
the top of the seal box is provided with a top cover which can be opened and closed, an auxiliary fixing plate and a purification column fixing plate are respectively horizontally arranged in the seal box from bottom to top, the auxiliary fixing plate is provided with a plurality of placing holes for placing centrifuge tubes, the purification column fixing plate is provided with a plurality of placing holes for placing purification columns, and the placing holes of the auxiliary fixing plate and the purification column fixing plate are corresponding to each other in the vertical direction; a cooling device is arranged between the auxiliary fixing plate and the purification column fixing plate on the inner side wall of the seal box;
the test tube is placed and is equipped with in the cabinet and place the board, places and has seted up a plurality of holes of placing on the board for place the centrifuging tube after the purification is accomplished.
But be equipped with height-adjusting's load post between above-mentioned auxiliary fixed plate and the purification post fixed plate, when the height of adjusting load post, change the distance between auxiliary fixed plate and the purification post fixed plate.
The support column is fixedly installed at the bottom of the base through spot welding, and protective paint is sprayed on the surfaces of the support column and the base.
The handle is fixedly arranged on the outer side of the storage box.
The edge of the top cover is fixed with a magnetic suction strip, and the corresponding position of the sealing box after the top cover is buckled is provided with an auxiliary magnetic suction strip matched with the magnetic suction strip.
The outside of above-mentioned seal box is equipped with the display screen, and the inside position of purifying column fixed plate top of seal box is equipped with temperature sensor, and temperature sensor shows the sealed incasement temperature transfer of sensing to the display screen.
The diameters of the placing holes of the auxiliary fixing plate and the purifying column fixing plate are increased from one side to the other side in sequence.
The cooling device comprises a shell and a driving motor fixed in the shell, wherein the output end of the driving motor is connected with a rotating fan blade, a refrigerator is arranged in front of the rotating fan blade, and the rotating fan blade blows cold air generated by the refrigerator into the sealed box.
The lower side of the auxiliary fixing plate in the sealing box is provided with a spongy cushion, and the spongy cushion is provided with a groove corresponding to the size corresponding to the placement hole.
After the scheme is adopted, the utility model discloses compare prior art's beneficial effect is:
(1) the utility model ensures that the purification column is vertically downward through the matching of the purification column fixing plate and the auxiliary fixing plate, so that the fixation process of the purification column is convenient and rapid, and the stable proceeding of the protein purification process is ensured;
(2) the utility model can prevent the protein from losing activity due to overhigh environmental temperature in the purification process by arranging the cooling device in the sealed box, and has good heat preservation effect and simple structure;
(3) the utility model can place different centrifuge tubes of different refrigeration devices according to the requirements by arranging different sizes of the placing holes on the purifying column fixing plate and the auxiliary fixing plate, thereby meeting different requirements and improving the competitiveness of the device;
(4) the utility model discloses a set up temperature sensor and display in the seal box, can carry out real-time detection to the inside cooling temperature of seal box to detect the temperature transfer and show for the display screen carries out real-time display, ensure that protein purification process remains the low temperature state throughout, improve the purification effect.
Drawings
Fig. 1 is a schematic perspective view of the present invention;
FIG. 2 is a schematic view of the internal structure of the middle sealing box of the present invention;
fig. 3 is an enlarged view of a in fig. 2.
Reference numerals:
1. a base; 2. a support pillar;
3. placing a bin; 4. a storage box;
5. a sealing box; 6. a top cover;
7. fixing the purification column; 8. an auxiliary fixing plate;
9. a cooling device; 10. a load column;
11. a test tube placing cabinet; 12. placing the plate; 13. placing holes;
14. a handle; 15. magnetically attracting the strip;
16. a display screen; 17. a temperature sensor;
18. placing the hole; 19. a housing; 20. a drive motor;
21. connecting columns; 22. rotating the fan blades; 23. a spongy cushion.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.
As shown in fig. 1 to 2, the utility model provides a fixing device for protein purification, which comprises a base 1, a support column 2 is fixedly arranged at the bottom of the base 1, a placing bin 3 is fixedly arranged at one side of the base 1, and a storage box 4 is fixedly arranged in the placing bin 3; a seal box 5 is fixedly installed at the top of the base 1, a top cover 6 is fixedly installed at the top of the seal box 5, a purification column fixing plate 7 is fixedly installed in the seal box 5, an auxiliary fixing plate 8 is fixedly installed at the bottom of the purification column fixing plate 7, a cooling device 9 is also fixedly installed at one side of the seal box 5 between the purification column fixing plate 7 and the auxiliary fixing plate 8, and a load column 10 is fixedly installed at one side of the seal box 5 far away from the cooling device 9; 5 one side fixed mounting of seal box has the test tube to place cabinet 11, and 11 top fixed mounting of cabinet are placed to the test tube have place board 12, place board 12 inside fixed being provided with and place hole 13.
As a preferred embodiment of the present invention, as shown in fig. 1, four support columns 2 are fixedly disposed at four corners of the bottom of the base 1, the support columns 2 are fixedly disposed at the bottom of the base 1 by spot welding, and the support columns 2 and the base 1 are coated with protective paint.
As a preferred embodiment of the present invention, as shown in fig. 1, the storage box 4 is slidably connected to the storage compartment 3 through a slide rail, a handle 14 is fixedly mounted on one side of the storage box 4, and a placement groove is fixedly mounted inside the storage box 4.
As a preferred embodiment of the present invention, as shown in fig. 1, the top cover 6 is fixedly mounted on one side of the top of the sealing box 5 through a hinge, the top of the top cover 6 is fixedly mounted with a magnetic attraction strip 15, and the top of the sealing box 5 is fixedly mounted with a magnetic attraction strip 15.
As a preferred embodiment of the present invention, as shown in fig. 1, a display screen 16 is fixedly installed on one side of the seal box 5, a temperature sensor 17 is fixedly installed inside the seal box 5 and on the top of the purification column fixing plate 7, and the temperature sensor 17 is electrically connected to the display screen 16.
As a preferred embodiment of the present invention, as shown in fig. 1 and fig. 2, the purifying column fixing plate 7 and the auxiliary fixing plate 8 are both fixedly provided with the placing holes 18, and the sizes of the placing holes 18 are sequentially increased.
As a preferred embodiment of the present invention, as shown in fig. 3, the cooling device 9 includes a housing 19, a driving motor 20 is fixedly mounted inside the housing 19, connecting posts 21 are fixedly mounted on both sides of the driving motor 20, a rotating fan 22 is fixedly mounted on one side of the driving motor 20, the output end of the driving motor 20 is in transmission connection with the rotating fan 22 through a shaft coupling, and a refrigerator is fixedly disposed on one side of the rotating fan 22.
As a preferred embodiment of the utility model, as shown in fig. 3, the inside fixed heat preservation that is provided with of seal box 5, 8 bottom fixed mounting of auxiliary fixed plate have a foam-rubber cushion 23, and the fixed recess that is provided with and puts 18 matched with in hole in foam-rubber cushion 23 top.
The utility model discloses a theory of operation is: a user opens the top cover 6 through a hinge, places the purification column to be processed in the placing hole 18 of the purification column fixing plate 7, then places the centrifugal tube in the placing hole 18 of the auxiliary fixing plate 8, the purification column is vertically downward, so that the purification column is quickly and simply fixed, and then adjusts the height of the load column 10 according to the length of the purification column, so that the bottom end of the purification column is matched with the upper end of the centrifugal tube; when carrying out the purification process, temperature sensor 17 carries out real-time detection to the inside cooling temperature of seal box 5 to will detect the temperature and transmit for display screen 16 and show in real time, cooling device 9 prevents that protein from losing the activity because of ambient temperature is too high in the purification process simultaneously, keeps warm effectually and simple structure, places the centrifuge tube after the purification is accomplished on placing the hole 13 of placing of board 12.
In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description of the present invention and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above should not be understood to necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples described in this specification can be combined and combined by those skilled in the art.
Although embodiments of the present invention have been shown and described, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art without departing from the scope of the present invention.
Claims (9)
1. The utility model provides a fixing device is used in protein purification which characterized in that: the test tube placing cabinet comprises a base, a sealing box and a test tube placing cabinet, wherein the sealing box and the test tube placing cabinet are arranged on the base;
the top of the seal box is provided with a top cover which can be opened and closed, an auxiliary fixing plate and a purification column fixing plate are respectively horizontally arranged in the seal box from bottom to top, the auxiliary fixing plate is provided with a plurality of placing holes for placing centrifuge tubes, the purification column fixing plate is provided with a plurality of placing holes for placing purification columns, and the placing holes of the auxiliary fixing plate and the purification column fixing plate are corresponding to each other in the vertical direction; a cooling device is arranged between the auxiliary fixing plate and the purification column fixing plate on the inner side wall of the seal box;
the test tube is placed and is equipped with in the cabinet and place the board, places and has seted up a plurality of holes of placing on the board for place the centrifuging tube after the purification is accomplished.
2. The protein purification fixation device according to claim 1, wherein: but be equipped with height-adjusting's load post between auxiliary fixed plate and the purification post fixed plate, when the height of adjusting load post, change the distance between auxiliary fixed plate and the purification post fixed plate.
3. The protein purification fixation device according to claim 1, wherein: the support column is fixedly installed at the bottom of the base through spot welding, and protective paint is sprayed on the surfaces of the support column and the base.
4. The protein purification fixation device according to claim 1, wherein: the handle is fixedly arranged on the outer side of the storage box.
5. The protein purification fixation device according to claim 1, wherein: the top cap edge is fixed with magnetism and inhales the strip, and the seal box is equipped with the supplementary strip of inhaling of strip matched with magnetism in the corresponding position behind the top cap lock.
6. The protein purification fixation device according to claim 1, wherein: the outside of seal box is equipped with the display screen, and the inside position of purifying column fixed plate top of seal box is equipped with temperature sensor, and temperature sensor shows the sealed incasement temperature transfer who senses.
7. The protein purification fixation device according to claim 1, wherein: the diameters of the placing holes of the auxiliary fixing plate and the purifying column fixing plate are increased from one side to the other side in sequence.
8. The protein purification fixation device according to claim 1, wherein: the cooling device comprises a shell and a driving motor fixed in the shell, the output end of the driving motor is connected with a rotating fan blade, a refrigerator is arranged in front of the rotating fan blade, and the rotating fan blade blows cold air generated by the refrigerator into the sealed box.
9. The protein purification fixation device according to claim 1, wherein: the lower side of the auxiliary fixing plate in the seal box is provided with a spongy cushion, and the spongy cushion is provided with a groove corresponding to the size corresponding to the placement hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022666027.8U CN213739254U (en) | 2020-11-18 | 2020-11-18 | Fixing device for protein purification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022666027.8U CN213739254U (en) | 2020-11-18 | 2020-11-18 | Fixing device for protein purification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213739254U true CN213739254U (en) | 2021-07-20 |
Family
ID=76825688
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022666027.8U Expired - Fee Related CN213739254U (en) | 2020-11-18 | 2020-11-18 | Fixing device for protein purification |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213739254U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113713878A (en) * | 2021-09-13 | 2021-11-30 | 深圳市雅为泓源生物科技有限公司 | Device for fixing and controlling temperature of purification column |
-
2020
- 2020-11-18 CN CN202022666027.8U patent/CN213739254U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113713878A (en) * | 2021-09-13 | 2021-11-30 | 深圳市雅为泓源生物科技有限公司 | Device for fixing and controlling temperature of purification column |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210720 |
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| CF01 | Termination of patent right due to non-payment of annual fee |