CN213680623U

CN213680623U - Nucleic acid extraction element who facilitates use

Info

Publication number: CN213680623U
Application number: CN202022199881.8U
Authority: CN
Inventors: 端青; 韩雪莲; 任宇豪; 杨江涛
Original assignee: Xiamen Health Medicine Co ltd
Current assignee: Xiamen Health Medicine Co ltd
Priority date: 2020-09-30
Filing date: 2020-09-30
Publication date: 2021-07-13
Anticipated expiration: 2030-09-30

Abstract

The utility model relates to the field of biotechnology, and a nucleic acid extraction element who facilitates use is disclosed, including handling the case, still include: the high-speed motor is arranged on the right side of the bottom of the inner wall of the treatment box, a conical gear is fixedly arranged on an output shaft of the high-speed motor, a gear disc is meshed on the right side of the conical gear, the bottom of the gear disc is rotatably connected with the bottom of the inner wall of the treatment box, a rotating rod is bolted to the top of the gear disc, a rotating box is fixedly connected to the top of the rotating rod, and the top end of the rotating box penetrates through the treatment box and is rotatably connected with the treatment box; the utility model discloses it is simple to possess the step of drawing, and work efficiency is high, and the installation is dismantled to the instrument of drawing to the convenience in addition, for the advantage that the operator facilitates, has solved current nucleic acid and has drawed equipment, and the step is more loaded down with trivial details, and degree of automation is low, makes work efficiency lower, and inconvenient dismantles the installation to drawing the instrument moreover, brings inconvenient problem for the operator.

Description

Nucleic acid extraction element who facilitates use

Technical Field

The utility model relates to the field of biotechnology, specifically be a nucleic acid extraction element who facilitates use.

Background

Nucleic acids are a class of biopolymers, an essential constituent of all known life forms; nucleic acids are a generic term for deoxyribonucleic acid (DNA) and ribonucleic acid (RNA); nucleic acids are composed of nucleotides, whereas nucleotide monomers are composed of 5-carbon sugars, phosphate groups and nitrogenous bases. If the 5-carbon sugar is ribose, the polymer formed is RNA; if the 5-carbon sugar is deoxyribose, the polymer formed is DNA.

The molecular biology detection technology is a crystal which makes great progress in modern molecular biology and molecular genetics, and is developed on the basis that people increasingly deepen understanding of essential life problems such as gene structure, gene expression, regulation and control and the like, nucleic acid extraction becomes the most important and basic operation in molecular biology experimental technology, and the extraction of nucleic acid is usually involved in the operation.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a nucleic acid extraction element who facilitates use, it is simple to possess the extraction step, and work efficiency is high, and the installation is dismantled to extracting the instrument to the convenience moreover, for the advantage that the operator facilitates, has solved current nucleic acid and has drawed equipment, and the step is more loaded down with trivial details, and degree of automation is low, makes work efficiency lower, and inconvenient dismantles the installation to extracting the instrument moreover, the problem of inconvenience is brought for the operator.

In order to achieve the above object, the utility model provides a following technical scheme: a nucleic acid extraction device that facilitates use, includes the processing case, still includes:

the high-speed motor is installed on the right side of the bottom of the inner wall of the processing box, a bevel gear is fixedly installed on an output shaft of the high-speed motor, a gear disc is meshed on the right side of the bevel gear, the bottom of the gear disc is rotatably connected with the bottom of the inner wall of the processing box, a rotating rod is bolted to the top of the gear disc, a rotating box is fixedly connected to the top of the rotating rod, the top end of the rotating box penetrates through the processing box and is rotatably connected with the processing box, a centrifugal tube is slidably connected to the inside of the rotating box, and clamping structures are arranged at the tops of the left side and the right side of;

the material pump is arranged at the top of the installation pipe, the left side and the right side of the installation pipe are both communicated with hoses, the bottom ends of the hoses are communicated with guide pipes, the middle parts of the left side and the right side of the support rod are fixedly connected with fixed rods, and one side of each fixed rod, which is far away from the support rod, is provided with a fixed structure;

the first box that draws, its setting is in the left side of handling the incasement wall, the first top that draws the box run through handle the case and with handle sliding connection between the case, the first bottom that draws the box is provided with nucleic acid filtration membrane, the first below that draws the box is provided with the second and draws the box, the second draws the bottom of box to be provided with the nucleic acid adsorption membrane, the second draws the below of box to be provided with the third and draws the box.

Preferably, the chucking structure is including fixed box, fixed block, spring and circular slot, fixed box bolt is at the top of rotatory box left and right sides, fixed block sliding connection is in the inside of fixed box, and one side of fixed block runs through fixed box and extends to the outside of fixed box, the fixed block passes through spring fixed connection with fixed box inner wall one side in opposite directions, the circular slot is seted up in the left and right sides of centrifuging tube, and sliding connection between fixed block and the circular slot.

Preferably, the fixing structure comprises a fixing plate, a first fixing plate and a screw rod, the fixing plate is bolted to one side of the fixing rod, which is far away from the support rod, the fixing plate is arc-shaped, the first fixing plate is bolted to the front side and the rear side of one side of the fixing plate, a threaded hole is formed in the surface of the first fixing plate, and the screw rod is in threaded connection with the inside of the threaded hole.

Preferably, the left and right sides of the first extraction box, the second extraction box and the third extraction box are all bolted with second fixing pieces, the surface of each second fixing piece is provided with a fixing groove, the inside of each fixing groove is connected with a pull rod in a sliding mode, and the bottom of each pull rod is bolted with a cross rod.

Preferably, the left side and the right side of the top of the second extraction box and the top of the third extraction box are both provided with sliding grooves, the bottoms of the first extraction box and the second extraction box are both bolted with sliding blocks, and the sliding grooves are in sliding connection with the sliding blocks.

Preferably, the inner wall of the fixing plate is bonded with a protection pad, and the protection pad is made of rubber.

Compared with the prior art, the beneficial effects of the utility model are as follows:

1. the inside of rotatory box is equipped with the centrifuging tube, and the top of installation pipe is equipped with the material pump, and the left and right sides of installation pipe all is equipped with the hose, and the first bottom that draws the box is equipped with nucleic acid filtration membrane, and the second bottom that draws the box is equipped with the nucleic acid adsorption film, has solved current nucleic acid and has drawed equipment, and the step is more loaded down with trivial details, problem that work efficiency hangs down, and the device possesses the advantage of drawing nucleic acid step simply, improvement work efficiency.

2. The left and right sides of rotatory box all is equipped with the chucking structure, and one side that the bracing piece was kept away from to the dead lever is equipped with fixed knot and constructs, has solved that current nucleic acid draws the equipment inconvenient dismantles the installation to drawing the instrument, and the device possesses the convenience and dismantles drawing the instrument, brings convenient advantage for the operator.

Drawings

FIG. 1 is a front sectional view of the structure of the present invention;

FIG. 2 is an enlarged view of a portion of the structure of FIG. 1;

FIG. 3 is an enlarged view of a portion of the structure of FIG. 1B according to the present invention;

fig. 4 is a schematic perspective view of the first extraction box of the present invention;

fig. 5 is a schematic perspective view of the fixing structure of the present invention.

In the figure: 1. a treatment tank; 2. a high-speed motor; 3. a bevel gear; 4. a gear plate; 5. rotating the rod; 6. rotating the cassette; 7. centrifuging the tube; 8. a clamping structure; 81. a fixing box; 82. a fixed block; 83. a spring; 84. a circular groove; 9. a support bar; 10. installing a pipe; 11. a material pump; 12. a hose; 13. a guide tube; 14. fixing the rod; 15. a fixed structure; 151. a fixing plate; 152. a first fixing sheet; 153. a screw; 16. a first extraction cartridge; 17. a nucleic acid filtration membrane; 18. a second extraction cartridge; 19. a nucleic acid-adsorbing membrane; 20. a third extraction cartridge; 21. a second fixing sheet; 22. fixing grooves; 23. a pull rod; 24. a cross bar; 25. a chute; 26. a slider; 27. and (6) a protective pad.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to FIGS. 1 to 5, a convenient nucleic acid isolation apparatus includes a processing chamber 1, and further includes:

the high-speed motor 2 is arranged on the right side of the bottom of the inner wall of the treatment box 1, a bevel gear 3 is fixedly arranged on an output shaft of the high-speed motor 2, a gear disc 4 is meshed on the right side of the bevel gear 3, the bottom of the gear disc 4 is rotatably connected with the bottom of the inner wall of the treatment box 1, a rotating rod 5 is bolted to the top of the gear disc 4, a rotating box 6 is fixedly connected to the top of the rotating rod 5, the top end of the rotating box 6 penetrates through the treatment box 1 and is rotatably connected with the treatment box 1, a centrifugal tube 7 is slidably connected to the inside of the rotating box 6, and clamping structures 8 are arranged at the tops of the;

the device comprises a supporting rod 9, a material pump 11, hoses 12, a guide pipe 13, a fixing rod 14 and a fixing structure 15, wherein the supporting rod 9 is fixedly arranged at the top of a treatment box 1, the top of the supporting rod 9 is bolted with a mounting pipe 10, the top of the mounting pipe 10 is provided with the material pump 11, the left side and the right side of the mounting pipe 10 are both communicated with the hoses 12, the bottom ends of the hoses 12 are communicated with the guide pipe 13, the middle parts of the left side and the right side of the supporting rod 9 are;

the first extraction box 16 is arranged on the left side of the inner wall of the treatment box 1, the top of the first extraction box 16 penetrates through the treatment box 1 and is connected with the treatment box 1 in a sliding manner, the nucleic acid filtering membrane 17 is arranged at the bottom of the first extraction box 16, the second extraction box 18 is arranged below the first extraction box 16, the nucleic acid adsorption membrane 19 is arranged at the bottom of the second extraction box 18, and the third extraction box 20 is arranged below the second extraction box 18.

Please refer to fig. 1 and 2, chucking structure 8 includes fixed box 81, fixed block 82, spring 83 and circular slot 84, fixed box 81 bolts at the top of the rotatory box 6 left and right sides, fixed block 82 sliding connection is in the inside of fixed box 81, and one side of fixed block 82 runs through fixed box 81 and extends to the outside of fixed box 81, fixed block 82 passes through spring 83 fixed connection with fixed box 81 inner wall one side in opposite directions, circular slot 84 sets up in the left and right sides of centrifuging tube 7, and sliding connection between fixed block 82 and the circular slot 84, through fixed box 81, fixed block 82, the setting of spring 83 and circular slot 84, can fix the position of centrifuging tube 7, prevent that centrifuging tube 7 from appearing rocking when carrying out centrifugal operation.

Referring to fig. 1 and 5, the fixing structure 15 includes a fixing plate 151, a first fixing plate 152 and a screw 153, the fixing plate 151 is bolted to one side of the fixing rod 14 away from the supporting rod 9, the fixing plate 151 is arc-shaped, the first fixing plate 152 is bolted to the front side and the rear side of one side of the fixing plate 151, a threaded hole is formed in the surface of the first fixing plate 152, the screw 153 is in threaded connection with the inside of the threaded hole, and the position of the guide tube 13 can be clamped and fixed by the arrangement of the fixing plate 151, the first fixing plate 152 and the screw 153.

Referring to fig. 1 and 4, the first extraction box 16, the second extraction box 18 and the third extraction box 20 are bolted with second fixing plates 21 at the left and right sides, fixing grooves 22 are formed on the surfaces of the second fixing plates 21, pull rods 23 are slidably connected inside the fixing grooves 22, cross rods 24 are bolted at the bottoms of the pull rods 23, and through the arrangement of the second fixing plates 21, the fixing grooves 22, the pull rods 23 and the cross rods 24, an operator can take out the extracted nucleic acids conveniently.

Referring to fig. 1, 3 and 4, the left and right sides of the top of the second extraction box 18 and the top of the third extraction box 20 are both provided with a sliding groove 25, the bottom of the first extraction box 16 and the bottom of the second extraction box 18 are both bolted with a sliding block 26, the sliding groove 25 is in sliding connection with the sliding block 26, and the stability between the three extraction boxes is improved by the arrangement of the sliding groove 25 and the sliding block 26.

Referring to fig. 5, a protection pad 27 is adhered to an inner wall of the fixing plate 151, the protection pad 27 is made of rubber, and the fixing plate 151 is arranged to prevent damage to the guide tube 13 when the guide tube 13 is clamped and fastened by the protection pad 27.

The working principle is as follows: when nucleic acid extraction is required, an operator turns on the high-speed motor 2, so that the high-speed motor 2 drives the bevel gear 3 to rotate, the bevel gear 3 is meshed with the gear disc 4, the gear disc 4 drives the rotating rod 5 to rotate, the centrifugal tube 7 starts centrifugal operation, cell rapid lysis is realized, preliminary extraction of nucleic acid is completed, the material pump 11 is started, the material pump 11 pumps the preliminarily extracted nucleic acid into the first extraction box 16 through the guide tube 13 on the right side, the preliminarily extracted nucleic acid flows downwards, impurities are filtered by the nucleic acid filtering membrane 17, the nucleic acid is adsorbed by the nucleic acid adsorption membrane 19 at the bottom of the second extraction box 18, then the screw 153 on the left side is rotated to detach the screw 153 from the first fixing plate 152, the guide tube 13 on the left side is pulled upwards, and the movement is stopped after the guide tube 13 moves upwards to a proper height, then the screw 153 is installed again to complete clamping and fixing of the guide tube 13, then the pull rod 23 is pulled upwards to take out the three extraction boxes, the pull rods 23 at two sides are rotated, when the cross rod 24 is overlapped with the fixing groove 22, the pull rod 23 is pulled upwards to complete dismounting of the pull rod 23, then the first extraction box 16 is pulled upwards, the second extraction box 18 is taken out, the nucleic acid adsorbed at the bottom of the second extraction box 18 is taken out, when the centrifugal tube 7 needs to be cleaned, the guide tube 13 at the right side is lifted upwards and fixed, then the centrifugal tube 7 is pulled upwards to enable the fixing block 82 to be moved out from the circular groove 84 and move towards the inside of the fixing box 81, the fixing block 82 extrudes the spring 83, when the centrifugal tube 7 is taken out, the spring 83 rebounds and pushes out the fixing block 82, after the centrifugal tube 7 is cleaned, the centrifugal tube 7 is put into the rotating box 6 again, the position of the centrifugal tube 7 is fixed, and the extraction of the nucleic acid is completed.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A nucleic acid extraction device that facilitates use, includes a processing tank (1), and is characterized by further comprising:

the high-speed motor (2) is installed on the right side of the bottom of the inner wall of the processing box (1), a bevel gear (3) is fixedly installed on an output shaft of the high-speed motor (2), a gear disc (4) is meshed on the right side of the bevel gear (3), the bottom of the gear disc (4) is rotatably connected with the bottom of the inner wall of the processing box (1), a rotating rod (5) is bolted to the top of the gear disc (4), a rotating box (6) is fixedly connected to the top of the rotating rod (5), the top end of the rotating box (6) penetrates through the processing box (1) and is rotatably connected with the processing box (1), a centrifugal tube (7) is slidably connected to the inside of the rotating box (6), and clamping structures (8) are arranged at the tops of the left side and the right side of the rotating box (;

the device comprises a supporting rod (9) which is fixedly arranged at the top of a treatment box (1), wherein the top of the supporting rod (9) is connected with a mounting pipe (10) in a bolted mode, the top of the mounting pipe (10) is provided with a material pump (11), the left side and the right side of the mounting pipe (10) are both communicated with hoses (12), the bottom end of each hose (12) is communicated with a guide pipe (13), the middle parts of the left side and the right side of the supporting rod (9) are fixedly connected with fixing rods (14), and one side, away from the supporting rod (9), of each fixing rod (14) is provided with a fixing structure;

the first box (16) of drawing, its setting is in the left side of handling case (1) inner wall, the top of first box (16) of drawing run through handle case (1) and with handle sliding connection between case (1), the bottom of first box (16) of drawing is provided with nucleic acid filtration membrane (17), the below of first box (16) of drawing is provided with the second and draws box (18), the bottom of second box (18) of drawing is provided with nucleic acid adsorption membrane (19), the below of second box (18) of drawing is provided with the third and draws box (20).

2. A convenient nucleic acid extraction device according to claim 1, wherein: chucking structure (8) are including fixed box (81), fixed block (82), spring (83) and circular slot (84), fixed box (81) bolt is at the top of rotatory box (6) left and right sides, fixed block (82) sliding connection is in the inside of fixed box (81), and one side of fixed block (82) runs through fixed box (81) and extends to the outside of fixed box (81), spring (83) fixed connection is passed through with fixed box (81) inner wall in opposite directions one side in fixed block (82), the left and right sides in centrifuging tube (7) is seted up in circular slot (84), and sliding connection between fixed block (82) and circular slot (84).

3. A convenient nucleic acid extraction device according to claim 1, wherein: fixed knot constructs (15) including fixed plate (151), first stationary blade (152) and screw rod (153), fixed plate (151) bolt is in one side of dead lever (14) keeping away from bracing piece (9), and fixed plate (151) are circular-arcly, first stationary blade (152) bolt is in the front and back both sides of fixed plate (151) one side, the screw hole has been seted up on the surface of first stationary blade (152), screw rod (153) threaded connection is in the inside of screw hole.

4. A convenient nucleic acid extraction device according to claim 1, wherein: the left side and the right side of the first extraction box (16), the second extraction box (18) and the third extraction box (20) are respectively bolted with a second fixing piece (21), a fixing groove (22) is formed in the surface of the second fixing piece (21), a pull rod (23) is connected to the inside of the fixing groove (22) in a sliding mode, and a cross rod (24) is bolted to the bottom of the pull rod (23).

5. A convenient nucleic acid extraction device according to claim 1, wherein: the left and right sides at the tops of the second extraction box (18) and the third extraction box (20) are respectively provided with a sliding groove (25), the bottoms of the first extraction box (16) and the second extraction box (18) are respectively bolted with a sliding block (26), and the sliding grooves (25) are in sliding connection with the sliding blocks (26).

6. A convenient nucleic acid extraction device according to claim 3, wherein: the inner wall of the fixing plate (151) is bonded with a protective pad (27), and the protective pad (27) is made of rubber materials.