CN213668036U

CN213668036U - Enzyme immobilization chromatographic column

Info

Publication number: CN213668036U
Application number: CN202022517617.4U
Authority: CN
Inventors: 李晓娟; 王珣; 李柱刚; 赵曦; 赵伟; 陆杰; 王家有; 冷春旭; 付玉杰; 赵春建; 盖庆岩; 焦骄
Original assignee: Biotechnology Research Institute Heilongjiang Academy Of Agricultural Sciences
Current assignee: Biotechnology Research Institute Heilongjiang Academy Of Agricultural Sciences
Priority date: 2020-11-04
Filing date: 2020-11-04
Publication date: 2021-07-13
Anticipated expiration: 2030-11-04

Abstract

The utility model discloses an enzyme immobilization chromatographic column, including the support body, set up in inside post pipe of support body, set up in the stationary phase dog-house on the post pipe lateral wall, set up in the top of post pipe stretches into downwards the inside mobile phase inlet pipe of post pipe, set up in the stationary phase discharge gate of post pipe bottom, and set up in the post pipe bottom is close to the fluid-discharge tube of stationary phase discharge gate one side, the inside slip of post pipe is provided with the clamp plate, be fixed with multiunit position sleeve on the bottom plate of support body, multiunit position sleeve's inside slip cap closes there is movable branch. The utility model discloses a vibrator can make the column tube carry out the vibrations of vertical direction, and transmission can drive arrangement simultaneously, makes the clamp plate receive perpendicular to effort extrusion downwards and loads in the inside stationary phase granule of column tube, and under the combined action of extrusion and vibrations, the stationary phase granule can evenly closely knit distribute in the column tube, the enzyme in more abundant absorption mobile phase.

Description

Enzyme immobilization chromatographic column

Technical Field

The utility model relates to a chromatographic column, in particular to an enzyme immobilization chromatographic column.

Background

Chromatography is a technique for achieving the separation purpose by utilizing different distribution proportions of substances between a stationary phase and a mobile phase, and the stationary phase is silica gel, activated carbon, alumina, ion exchange resin, ion exchange fiber and the like.

The existing enzyme immobilization chromatographic column has no compaction measure aiming at stationary phase particles, the accumulation of the stationary phase particles is loose and not uniform enough, and the gaps among the particles are large, so that the enzyme absorption in the stationary phase relative to the mobile phase is not sufficient.

SUMMERY OF THE UTILITY MODEL

Based on the above mentioned shortcomings in the prior art, the utility model provides an enzyme immobilization chromatographic column.

The utility model discloses an adopt following technical scheme to overcome above technical problem, specifically do:

an enzyme immobilized chromatographic column comprises a frame body, a column tube arranged in the frame body, a stationary phase feed port arranged on the side wall of the column tube, a mobile phase feed pipe arranged at the top end of the column tube and extending downwards into the column tube, a stationary phase discharge port arranged at the bottom end of the column tube, and a liquid discharge pipe arranged at the bottom end of the column tube and close to one side of the stationary phase discharge port, wherein a pressing plate is arranged in the column tube in a sliding manner, a plurality of groups of positioning sleeves are fixed on a bottom plate of the frame body, movable support rods are sleeved in the plurality of groups of positioning sleeves in a sliding manner, a support plate is fixed at the top end of each movable support rod, supports are fixed on two sides of the column tube and are fixedly connected with the support plates, a servo motor is fixed on the bottom plate of the frame body, and a first rotating shaft which is rotatably connected with a side plate of the frame, a second rotating shaft which is rotatably connected with the side plate of the frame body is arranged above the column tube;

the device comprises a support plate, a first rotating shaft, a second rotating shaft, a column tube, a pressing plate, a driving device and a transmission device, wherein a vibration device acting on the support plate is arranged between the first rotating shaft and the support plate, a guide device for the pressing plate to slide up and down is arranged inside the column tube, the driving device acting on the pressing plate is arranged between the pressing plate and the second rotating shaft, and the transmission device is arranged between the first rotating shaft and the second rotating shaft.

As a further aspect of the present invention: the vibration device comprises a grooved rotary table fixedly sleeved on the first rotating shaft, a roller rotatably arranged at the bottom end of the support plate and in rolling contact with the surface of the grooved rotary table, a baffle fixed at the bottom end of the movable supporting rod and a spring sleeved on the surface of the movable supporting rod and in a stressed and compressed state;

the baffle with the spring all set up in positioning sleeve's inside, the one end of spring with the baffle is kept away from the one end of support body bottom plate is inconsistent, the other end of spring with positioning sleeve's inner wall is inconsistent.

As a further aspect of the present invention: the guiding device comprises a sliding rod fixed inside the column tube and a sliding sleeve which is sleeved on the sliding rod in a sliding mode and fixedly connected with the pressing plate.

As a further aspect of the present invention: the driving device comprises a first gear fixed at the top end of the pressing plate and extending upwards out of the top plate of the column tube, and a rack fixedly sleeved on the surface of the second rotating shaft and meshed with the first gear.

As a further aspect of the present invention: the transmission device comprises a second gear fixedly sleeved on the first rotating shaft, a third gear fixedly sleeved on the second rotating shaft, and an inner belt sleeved between the second gear and the third gear;

the top end and the bottom end of the inner ruler belt are respectively meshed with the second gear and the third gear.

As a further aspect of the present invention: the clamp plate is provided with a through hole for inserting one end of the mobile phase feeding pipe, and a filter screen is arranged inside the through hole.

After the structure more than adopting, the utility model discloses compare in prior art, possess following advantage: can make the column casing carry out the vibrations of vertical direction through vibrator, transmission can drive arrangement simultaneously, makes the clamp plate receive vertical to effort and extrudees downwards and pack in the inside stationary phase granule of column casing, and under the combined action of extrusion and vibrations, the stationary phase granule can be evenly closely knit distributes in the column casing, the enzyme in more abundant absorption mobile phase.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a schematic structural diagram of the middle baffle and the spring of the present invention.

Fig. 3 is a schematic structural view of the turntable with grooves and the roller in the present invention.

Fig. 4 is a schematic structural diagram of the second gear and the third gear of the present invention.

Fig. 5 is a schematic structural view of the positioning sleeve and the support plate of the present invention.

In the figure: 1. a frame body; 2. a column tube; 3. a stationary phase feed port; 4. a mobile phase feed pipe; 5. a stationary phase discharge port; 6. a liquid discharge pipe; 7. pressing a plate; 8. a positioning sleeve; 9. a movable strut; 10. a support plate; 11. a support; 12. a servo motor; 13. a first rotating shaft; 14. a second rotating shaft; 15. a turntable with a groove; 16. a roller; 17. a baffle plate; 18. a spring; 19. a slide rod; 20. a sliding sleeve; 21. a first gear; 22. a rack; 23. a second gear; 24. a third gear; 25. an inner ruler belt; 26. a through hole; 27. and (5) filtering by using a filter screen.

Detailed Description

In order to facilitate understanding of the present invention, the present invention will be described more fully hereinafter with reference to the accompanying drawings. The preferred embodiments of the present invention are shown in the drawings. The invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

In addition, an element of the present invention may be said to be "secured to" or "disposed on" another element, either directly on the other element or with intervening elements present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only and do not represent the only embodiments.

Referring to fig. 1 to 5, in an embodiment of the present invention, an enzyme immobilized chromatographic column includes a frame body 1, a column tube 2 disposed inside the frame body 1, a stationary phase feeding port 3 disposed on a side wall of the column tube 2, a mobile phase feeding pipe 4 disposed at a top end of the column tube 2 and extending downward into the column tube 2, a stationary phase discharging port 5 disposed at a bottom end of the column tube 2, and a liquid discharging pipe 6 disposed at a bottom end of the column tube 2 near a side of the stationary phase discharging port 5, a pressing plate 7 slidably disposed inside the column tube 2, a plurality of sets of positioning sleeves 8 fixed on a bottom plate of the frame body 1, a movable support rod 9 slidably sleeved inside the plurality of sets of positioning sleeves 8, a support plate 10 fixed at a top end of the movable support rod 9, supports 11 fixed at two sides of the column tube 2, the supports 11 fixedly connected to the support plate 10, a servo motor 12 is fixed on a bottom plate of the frame body 1, a first rotating shaft 13 which is rotatably connected with a side plate of the frame body 1 is fixed on an output shaft of the servo motor 12, and a second rotating shaft 14 which is rotatably connected with the side plate of the frame body 1 is arranged above the column tube 2;

a vibration device acting on the support plate 10 is arranged between the first rotating shaft 13 and the support plate 10, a guide device for the pressing plate 7 to slide up and down is arranged inside the column tube 2, a driving device acting on the pressing plate 7 is arranged between the pressing plate 7 and the second rotating shaft 14, and a transmission device is arranged between the first rotating shaft 13 and the second rotating shaft 14;

the stationary phase dog-house 3 is opened to fill stationary phase particles into the column tube 2, the enzyme-containing solution flows into the column tube 2 from the mobile phase inlet tube 4, the flowing speed of the mobile phase can be adjusted by controlling the adjusting valve on the mobile phase inlet tube 4, the output shaft of the servo motor 12 can drive the first rotating shaft 13 to rotate, the support plate 10 can perform reciprocating vibration in the vertical direction under the action of the vibration device when the first rotating shaft 13 rotates, so as to drive the column tube 2 to perform vertical vibration integrally, the stationary phase particles inside the column tube 2 are distributed more uniformly, the first rotating shaft 13 can drive the transmission device to rotate the second rotating shaft 14 when rotating, when the second rotating shaft 14 rotates, the pressure plate 7 can be driven by the driving device to vertically move in the column tube 2 along the direction of the guiding device, when the pressure plate 7 moves downwards, the stationary phase particles inside the column tube 2 can be extruded downwards, the stationary phase granule of column tube 2 inside can be under vibrations and extruded combined action, and the distribution is more even and closely knit, helps improving chromatography efficiency.

In an embodiment of the present invention, the vibration device includes a grooved rotary table 15 fixedly sleeved on the first rotary shaft 13, a roller 16 rotatably disposed at the bottom end of the support plate 10 and in rolling contact with the surface of the grooved rotary table 15, a baffle 17 fixed at the bottom end of the movable rod 9, and a spring 18 sleeved on the surface of the movable rod 9 and in a stressed and compressed state;

the baffle 17 and the spring 18 are both arranged inside the positioning sleeve 8, one end of the spring 18 is abutted against one end of the baffle 17 far away from the bottom plate of the frame body 1, and the other end of the spring 18 is abutted against the inner wall of the positioning sleeve 8;

because the spring 18 is in a stressed and compressed state, the baffle 17 can be acted by the downward elastic force of the spring 18, the baffle 17 can drive the grooved turntable 15 to generate downward pulling force on the support plate 10, so that the roller 16 hinged at the bottom end of the support plate 10 is in close contact with the groove on the surface of the grooved turntable 15, the first rotating shaft 13 can drive the grooved turntable 15 to rotate when rotating, and the roller 16 is tightly attached to the groove on the surface of the grooved turntable 15 to roll and drive the support plate 10 to perform reciprocating vibration in the vertical direction.

In another embodiment of the present invention, the guiding device comprises a sliding rod 19 fixed inside the column tube 2, and a sliding sleeve 20 slidably sleeved on the sliding rod 19 and fixedly connected to the pressing plate 7;

when the pressing plate 7 is forced to move, the sliding sleeve 20 slides on the surface of the sliding rod 19 along the vertical direction, and at this time, the pressing plate 7 can move along the vertical direction under the restriction of the sliding rod 19 and the sliding sleeve 20.

In another embodiment of the present invention, the driving device comprises a first gear 21 fixed on the top end of the pressing plate 7 and extending upward out of the top plate of the column tube 2, and a rack 22 fixedly attached to the surface of the second rotating shaft 14 and engaged with the first gear 21;

when the second rotating shaft 14 rotates, the first gear 21 is driven to rotate, and due to the meshing relationship between the rack 22 and the first gear 21, the first gear 21 rotates and simultaneously generates a driving force in a vertical direction on the rack 22, so that the platen 7 is driven to move vertically.

In another embodiment of the present invention, the transmission device includes a second gear 23 fixed on the first shaft 13, a third gear 24 fixed on the second shaft 14, and an inner belt 25 sleeved between the second gear 23 and the third gear 24;

wherein, the top end and the bottom end of the inner belt 25 are respectively meshed with the second gear 23 and the third gear 24;

the first rotating shaft 13 drives the second gear 23 to rotate, the third gear 24 is driven by the inner belt 25 to rotate, finally the second rotating shaft 14 is driven by the third gear 24 to rotate, and the diameter of the second gear 23 is smaller than that of the third gear 24, so that the rotating speed of the second gear 23 is slower than that of the third gear 24.

In another embodiment of the present invention, a through hole 26 is formed on the pressing plate 7 for inserting one end of the mobile phase feeding pipe 4, and a filter screen 27 is disposed inside the through hole 26;

the enzyme solution flowing out from the mobile phase feeding pipe 4 can be dripped into the column tube 2 through the through hole 26, and the filter screen 27 can prevent the pressing plate 7 from entering the inside of the through hole 26 when the stationary phase particles inside the column tube 2 are compressed, so as to block the through hole 26.

The foregoing is illustrative of the preferred embodiments of the present invention only, and is not to be construed as limiting the claims. The present invention is not limited to the above embodiments, and the specific structure thereof is allowed to be changed. However, all changes which come within the scope of the independent claims of the invention are to be embraced therein.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

Claims

1. An enzyme immobilized chromatographic column comprises a frame body (1), a column tube (2) arranged in the frame body (1), a stationary phase feed port (3) arranged on the side wall of the column tube (2), a mobile phase feed pipe (4) arranged at the top end of the column tube (2) and extending downwards into the column tube (2), a stationary phase discharge port (5) arranged at the bottom end of the column tube (2), and a liquid discharge pipe (6) arranged at the bottom end of the column tube (2) and close to one side of the stationary phase discharge port (5), and is characterized in that a pressing plate (7) is arranged in the column tube (2) in a sliding manner, a plurality of groups of positioning sleeves (8) are fixed on a bottom plate of the frame body (1), movable support rods (9) are sleeved in the plurality of groups of positioning sleeves (8) in a sliding manner, support plates (10) are fixed at the top ends of the movable support rods (9), two sides of the column tube (2) are fixedly provided with a support (11), the support (11) is fixedly connected with the support plate (10), a bottom plate of the frame body (1) is fixedly provided with a servo motor (12), an output shaft of the servo motor (12) is fixedly provided with a first rotating shaft (13) which is rotatably connected with a side plate of the frame body (1), and a second rotating shaft (14) which is rotatably connected with the side plate of the frame body (1) is arranged above the column tube (2);

the device comprises a support plate (10), a first rotating shaft (13) and a second rotating shaft (14), wherein a vibration device acting on the support plate (10) is arranged between the first rotating shaft (13) and the support plate (10), a guide device for enabling the pressing plate (7) to slide up and down is arranged inside the column tube (2), a driving device acting on the pressing plate (7) is arranged between the pressing plate (7) and the second rotating shaft (14), and a transmission device is arranged between the first rotating shaft (13) and the second rotating shaft (14).

2. The enzyme immobilization chromatography column according to claim 1, wherein the vibration device comprises a grooved turntable (15) fixedly sleeved on the first rotating shaft (13), a roller (16) rotatably disposed at the bottom end of the support plate (10) and in rolling contact with the surface of the grooved turntable (15), a baffle (17) fixed at the bottom end of the movable support rod (9), and a spring (18) sleeved on the surface of the movable support rod (9) and under a stressed compression state;

wherein, baffle (17) with spring (18) all set up in the inside of position sleeve (8), the one end of spring (18) with baffle (17) are kept away from the one end of support body (1) bottom plate is inconsistent, the other end of spring (18) with the inner wall of position sleeve (8) is inconsistent.

3. The enzyme-immobilized chromatography column according to claim 1, wherein the guide means comprises a slide rod (19) fixed inside the column tube (2), and a slide sleeve (20) slidably fitted over the slide rod (19) and fixedly connected to the pressure plate (7).

4. An enzyme-immobilized chromatography column according to claim 1, wherein the driving means comprises a first gear (21) fixed to the top end of the pressure plate (7) and extending upward from the top plate of the column tube (2), and a rack (22) fixed to the surface of the second shaft (14) and engaged with the first gear (21).

5. The enzyme immobilization chromatography column as claimed in claim 1, wherein said transmission means comprises a second gear (23) fixedly secured to said first shaft (13), a third gear (24) fixedly secured to said second shaft (14), and an inner belt (25) fitted between said second gear (23) and said third gear (24);

wherein the top end and the bottom end of the inner belt (25) are respectively meshed with the second gear (23) and the third gear (24).

6. The enzyme-immobilized chromatographic column according to claim 1, wherein the pressure plate (7) is provided with a through hole (26) into which one end of the mobile phase feed pipe (4) is inserted, and a filter screen (27) is arranged inside the through hole (26).