CN213623937U

CN213623937U - High-precision protein purification device for purifying hepatitis B e antigen

Info

Publication number: CN213623937U
Application number: CN202021904919.0U
Authority: CN
Inventors: 刘杰; 黄杰良
Original assignee: Xiamen Wanbo Biotechnology Co ltd
Current assignee: Xiamen Wanbo Biotechnology Co ltd
Priority date: 2020-09-03
Filing date: 2020-09-03
Publication date: 2021-07-06
Anticipated expiration: 2030-09-03

Abstract

The utility model discloses a high accuracy protein purification device of purification hepatitis B e antigen, including first bottom plate, the upper end of first bottom plate is fixed with the bracing piece, the bracing piece symmetry is provided with two, the upper end of bracing piece is fixed with the second bottom plate, the outside of first bottom plate is fixed with the head rod, the top of head rod is fixed with first montant, the outside of first montant is fixed with the second connecting rod, first montant passes through second connecting rod and second bottom plate interconnect, the outside of first montant is fixed with the third connecting rod, the top of third connecting rod is fixed with the first lantern ring, the bottom of second bottom plate is provided with first motor. This high accuracy protein purification device of purification hepatitis B e antigen starts first motor and drives the rotation of second montant, and the second montant drives the rolling disc and rotates, makes another test tube connect another layer of solution after the analysis, has realized automatic accurate taking processing to the protein solution after the purification.

Description

High-precision protein purification device for purifying hepatitis B e antigen

Technical Field

The utility model relates to a protein purification equipment technical field specifically is a high accuracy protein purification device of purification hepatitis B e antigen.

Background

Protein purification equipment is widely used in biochemical research and application, is an important operation technology, a typical eukaryotic cell can contain thousands of different proteins, some are abundant in content, some only contain a few copies, in order to research a certain protein, the protein must be purified from other proteins and non-protein molecules at first, when the existing protein purification device is used for purifying the protein, the purified protein cannot be contained in time, and when a liquid is manually taken, other solutions are easy to enter, so that the purified liquid is not pure enough.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a high accuracy protein purification device of purification hepatitis B e antigen to solve the problem that current protein purification device that above-mentioned background art provided can not carry out automatic connecing to the protein solution after the purification and get.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a high accuracy protein purification device of purification hepatitis B e antigen, includes first bottom plate, the upper end of first bottom plate is fixed with the bracing piece, the bracing piece symmetry is provided with two, the upper end of bracing piece is fixed with the second bottom plate, the outside of first bottom plate is fixed with the head rod, the top of head rod is fixed with first montant, the outside of first montant is fixed with the second connecting rod, first montant passes through second connecting rod and second bottom plate interconnect, the outside of first montant is fixed with the third connecting rod, the top of third connecting rod is fixed with the first lantern ring, the bottom of second bottom plate is provided with first motor.

Preferably, a second vertical rod is arranged at the upper end of the first motor and is rotatably connected with the second bottom plate, a second motor is arranged at the upper end of the second vertical rod, and a first sliding groove is formed in the outer side of the second vertical rod.

Preferably, the inside of second montant is provided with the threaded rod, the upper end of threaded rod and the bottom interconnect of second motor, the outside of threaded rod is provided with the second lantern ring, the threaded rod is connected for the lead screw with the second lantern ring, the outside of the second lantern ring is fixed with the fourth connecting rod, first spout is sliding connection with the fourth connecting rod.

Preferably, a rotating disc is fixed on the outer side of the fourth connecting rod, the rotating disc is in sliding connection with the second vertical rod, a second sliding groove is formed in the upper end of the rotating disc, and the second sliding grooves are symmetrically formed in a plurality of positions.

Preferably, the inboard of second spout is provided with the test tube, the second spout is sliding connection with the test tube, the outside upper end of test tube is fixed with first baffle, the diameter of first baffle is greater than the diameter of second spout.

Preferably, the inboard of first lantern ring is provided with a purification section of thick bamboo, first lantern ring and a purification section of thick bamboo are sliding connection, the bottom mounting of a purification section of thick bamboo has the liquid outlet, the outside of a purification section of thick bamboo is fixed with the second baffle, the diameter ratio of second baffle is big than the diameter of a purification section of thick bamboo, the upper end of a purification section of thick bamboo is provided with the cover, the cover is connected for the block with a purification section of thick bamboo, the inboard of a purification section of thick bamboo is provided with the screening layer, screening layer symmetry is provided with two.

Compared with the prior art, the beneficial effects of the utility model are that: this high accuracy protein purification device of purification hepatitis B e antigen, through putting the screening layer into a purification section of thick bamboo, then put into the protein solution that needs the purification, put into another screening layer after, add the solution that the PH is suitable, close cover and purification section of thick bamboo, slide in the purification section of thick bamboo along first lantern ring, first lantern ring receives the hindrance of second baffle, fix purification section of thick bamboo position, slide in the test tube along the second spout, the second spout receives the hindrance of first baffle, carry out the rigidity to the test tube, start the second motor and drive the threaded rod and rotate, it moves upwards to drive the second lantern ring, the second lantern ring drives the fourth connecting rod and moves upwards, the fourth connecting rod drives the rolling disc and moves upwards to suitable position, the protein in the purification section of thick bamboo carries out the purification process, the solution after the analysis gets into the test tube, start first motor and drive the rotation of second montant, the second montant drives the rolling disc and rotates, and another test tube is connected with another layer of solution after analysis, so that the automatic and accurate connection and taking treatment of the purified protein solution is realized.

Drawings

FIG. 1 is a schematic perspective view of the present invention;

fig. 2 is a schematic view of the unfolding structure of the present invention;

fig. 3 is a schematic view of the internal structure of the second vertical rod of the present invention;

fig. 4 is a schematic view of the cross-sectional structure of the purifying cartridge of the present invention.

In the figure: 1. a first base plate; 2. a support bar; 3. a second base plate; 4. a first connecting rod; 5. a first vertical bar; 6. a second connecting rod; 7. a third connecting rod; 8. a first collar; 9. a first motor; 10. a second vertical bar; 11. a second motor; 12. a first chute; 13. a threaded rod; 14. a second collar; 15. a fourth connecting rod; 16. rotating the disc; 17. a second chute; 18. a test tube; 19. a first baffle plate; 20. a purification cartridge; 21. a liquid outlet; 22. a second baffle; 23. a cylinder cover; 24. and (5) screening layers.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-4, the present invention provides a technical solution: the utility model provides a high accuracy protein purification device of purification hepatitis B e antigen, including first bottom plate 1, the upper end of first bottom plate 1 is fixed with bracing piece 2, bracing piece 2 symmetry is provided with two, the upper end of bracing piece 2 is fixed with second bottom plate 3, the outside of first bottom plate 1 is fixed with head rod 4, the top of head rod 4 is fixed with first montant 5, the outside of first montant 5 is fixed with second connecting rod 6, first montant 5 passes through second connecting

rod

6 and 3 interconnect of second bottom plate, the outside of first montant 5 is fixed with third connecting rod 7, the top of third connecting rod 7 is fixed with first lantern ring 8, the bottom of second bottom plate 3 is provided with first motor 9.

Further, the upper end of the first motor 9 is provided with a second vertical rod 10, the second vertical rod 10 is rotatably connected with the second bottom plate 3, the upper end of the second vertical rod 10 is provided with a second motor 11, and the outer side of the second vertical rod 10 is provided with a first sliding groove 12, so that the rotation of the rotating disc 16 is realized.

Further, the inside of second montant 10 is provided with threaded rod 13, the upper end of threaded rod 13 and the bottom interconnect of second motor 11, and the outside of threaded rod 13 is provided with the second lantern ring 14, and threaded rod 13 is connected for the lead screw with second lantern ring 14, and the outside of second lantern ring 14 is fixed with fourth connecting rod 15, and first spout 12 is sliding connection with fourth connecting rod 15, has realized handling from top to bottom to rolling disc 16.

Further, the outside of fourth connecting rod 15 is fixed with rolling disc 16, and rolling disc 16 is sliding connection with second montant 10, and second spout 17 has been seted up to rolling disc 16's upper end, and second spout 17 symmetry is provided with a plurality ofly, has realized placing more test tubes 18, and more accurate connecing gets solution.

Further, the inboard of second spout 17 is provided with test tube 18, and second spout 17 is sliding connection with test tube 18, and test tube 18's outside upper end is fixed with first baffle 19, and the diameter of first baffle 19 is greater than the diameter of second spout 17, is convenient for to putting into and taking out of test tube 18.

Further, the inboard of first lantern ring 8 is provided with a purification section of thick bamboo 20, first lantern ring 8 is sliding connection with a purification section of thick bamboo 20, the bottom mounting of a purification section of thick bamboo 20 has liquid outlet 21, the outside of a purification section of thick bamboo 20 is fixed with second baffle 22, the diameter ratio of second baffle 22 is big than the diameter of a purification section of thick bamboo 20, the upper end of a purification section of thick bamboo 20 is provided with cover 23, cover 23 is connected for the block with a purification section of thick bamboo 20, the inboard of a purification section of thick bamboo 20 is provided with screening layer 24, screening layer 24 symmetry is provided with two, the purification treatment to protein has been realized.

The working principle is as follows: the screening layer 24 is put into the purifying cylinder 20, then the protein solution to be purified is put into the purifying cylinder, after another screening layer 24 is put into the purifying cylinder, the solution with proper PH is added, the cylinder cover 23 and the purifying cylinder 20 are closed, the purifying cylinder 20 slides in along the first lantern ring 8, the first lantern ring 8 is blocked by the second baffle 22, the position of the purifying cylinder 20 is fixed, the test tube 18 slides in along the second chute 17, the second chute 17 is blocked by the first baffle 19, the test tube 18 is fixed in position, the second motor 11 is started to drive the threaded rod 13 to rotate, the threaded rod 13 drives the second lantern ring 14 to move upwards, the second lantern ring 14 drives the fourth connecting rod 15 to move upwards, the fourth connecting rod 15 drives the rotating disc 16 to move upwards to a proper position, the protein in the purifying cylinder 20 is purified, the analyzed solution enters the test tube 18, the first motor 9 is started to drive the second vertical rod 10 to rotate, the second vertical rod 10 drives the rotating disc 16 to rotate, so that the other test tube 18 is connected with the other layer of solution after analysis, and the automatic and accurate connection and taking treatment of the purified protein solution is realized.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a high accuracy protein purification device of purification hepatitis B e antigen, includes first bottom plate, its characterized in that: the utility model discloses a motor, including first bottom plate, bracing piece, first bottom plate, second bottom plate, first connecting rod, second connecting rod, first montant, first bottom plate, first connecting rod, second connecting rod, first motor, second motor, first bottom plate, the upper end of first bottom plate is fixed with the bracing piece, the bracing piece symmetry is provided with two, the upper end of bracing piece is fixed with the second bottom plate, the outside of first bottom plate is fixed with the head rod, the top of head rod is fixed with first montant, the outside of first montant is fixed with the second connecting rod, first montant passes through second connecting rod and second bottom plate interconnect, the outside of first montant is fixed with the third connecting.

2. The apparatus for purifying hepatitis B e antigen with high precision according to claim 1, wherein: the upper end of the first motor is provided with a second vertical rod, the second vertical rod is rotatably connected with the second bottom plate, the upper end of the second vertical rod is provided with a second motor, and a first sliding groove is formed in the outer side of the second vertical rod.

3. The apparatus for purifying hepatitis B e antigen with high precision according to claim 2, wherein: the utility model discloses a motor, including first montant, second montant, upper end and the bottom interconnect of second motor, the inside of second montant is provided with the threaded rod, the upper end of threaded rod and the bottom interconnect of second motor, the outside of threaded rod is provided with the second lantern ring, the threaded rod is connected for the lead screw with the second lantern ring, the outside of the second lantern ring is fixed with the fourth connecting rod, first spout is sliding connection with the fourth connecting rod.

4. The apparatus for purifying hepatitis B e antigen with high precision according to claim 3, wherein: the outer side of the fourth connecting rod is fixed with a rotating disc, the rotating disc is in sliding connection with the second vertical rod, a second sliding groove is formed in the upper end of the rotating disc, and the second sliding grooves are symmetrically formed in a plurality of positions.

5. The apparatus for purifying hepatitis B e antigen with high precision according to claim 4, wherein: the inboard of second spout is provided with the test tube, the second spout is sliding connection with the test tube, the outside upper end of test tube is fixed with first baffle, the diameter of the diameter ratio second spout of first baffle is big.

6. The apparatus for purifying hepatitis B e antigen with high precision according to claim 1, wherein: the inboard of first lantern ring is provided with a purification section of thick bamboo, first lantern ring is sliding connection with a purification section of thick bamboo, the bottom mounting of a purification section of thick bamboo has the liquid outlet, the outside of a purification section of thick bamboo is fixed with the second baffle, the diameter ratio of second baffle is big than the diameter of a purification section of thick bamboo, the upper end of a purification section of thick bamboo is provided with the cover, the cover is connected for the block with a purification section of thick bamboo, the inboard of a purification section of thick bamboo is provided with the screening layer, screening layer symmetry is provided with two.