CN213570526U - Tissue cell culture bottle - Google Patents
Tissue cell culture bottle Download PDFInfo
- Publication number
- CN213570526U CN213570526U CN202020536151.XU CN202020536151U CN213570526U CN 213570526 U CN213570526 U CN 213570526U CN 202020536151 U CN202020536151 U CN 202020536151U CN 213570526 U CN213570526 U CN 213570526U
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- CN
- China
- Prior art keywords
- bottle
- box body
- culture
- pipe
- culture bottle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 238000004113 cell culture Methods 0.000 title claims abstract description 12
- 239000007788 liquid Substances 0.000 claims abstract description 31
- 238000007789 sealing Methods 0.000 claims abstract description 21
- 125000003003 spiro group Chemical group 0.000 claims description 2
- 238000007599 discharging Methods 0.000 claims 3
- 239000011550 stock solution Substances 0.000 claims 2
- 210000003701 histiocyte Anatomy 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 description 12
- 239000001963 growth medium Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000012466 permeate Substances 0.000 description 2
- 206010066054 Dysmorphism Diseases 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 239000012510 hollow fiber Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a tissue cell culture bottle, which comprises a box body, wherein the box body is internally provided with a culture bottle, the side wall of the culture bottle is symmetrically provided with an operation tube, one end of the operation tube respectively penetrates through the side wall of the culture bottle, the other end of the operation tube respectively penetrates through the side wall of the box body, the upper end of the culture bottle is screwed with a second sealing cover, the lower end of the side wall of the box body is provided with one end of a liquid discharge tube, the liquid discharge tube is provided with a first valve, the upper end of the side wall of the box body is provided with a liquid feed tube, the liquid feed tube is provided with a second valve, when the culture solution is replaced, the culture solution in the box body is discharged only through the liquid discharge tube arranged on the box body, when the culture solution is required to be added, the culture solution can be added through the liquid feed tube, and the operation tube is communicated with the culture, thereby both hands can infiltrate in the operation pipe, operate the histiocyte in the blake bottle, convenient to use and simple structure.
Description
Technical Field
The utility model relates to a cell culture technical field, specific field is a tissue cell culture bottle.
Background
Tissue culture is a technique in which living isolated organs (such as roots, stems, leaves, stem segments, protoplasts), tissues or cells are placed in a culture medium under artificially created sterile conditions and placed in an appropriate environment for continuous culture to obtain cells, tissues or individuals. The culture bottle is a common device in the technology, the traditional tissue cell culture bottle is of a single bottle structure of an original flat overhead bottle, when the culture solution is changed, the tissue cells need to be separated out in a centrifugal mode, then the original solution is discarded, new culture solution is changed in, pollution is inevitably caused in the process, the culture effect of the tissue cells is influenced, meanwhile, the solution changing method is time-consuming and labor-consuming, the cost in multiple aspects is high, meanwhile, the bottleneck is small, the tissue is extremely inconvenient to slice, peel and the like, and therefore the tissue cell culture bottle is provided.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a tissue cell culture bottle to solve the problem that proposes among the above-mentioned background art.
In order to achieve the above object, the utility model provides a following technical scheme: a tissue cell culture bottle comprises a cylindrical box body, a culture bottle is arranged in the box body, the culture bottle is cylindrical, the culture bottle and the box body are coaxially arranged, the side wall of the culture bottle is symmetrically provided with operating tubes, one end of each operating tube penetrates through the side wall of the culture bottle, the other end of the operating pipe respectively penetrates through the side wall of the box body, the side wall of the other end of the operating pipe is respectively hinged with a first sealing cover, the outer surface of the operating pipe is hinged with a convex hook respectively far away from the corresponding hinged part of the first sealing cover, the convex hook is matched with the first sealing cover, the upper end of the culture bottle is screwed with a second sealing cover, the lower end of the side wall of the box body is provided with one end of a liquid discharge pipe, the liquid discharge pipe is provided with a first valve, the upper end of the side wall of the box body is provided with a liquid inlet pipe, and the liquid inlet pipe is provided with a second valve.
Preferably, the operating tubes are all arranged obliquely, and one end of each operating tube, which is far away from the culture bottle, is higher than one end of each operating tube, which is close to the culture bottle.
Preferably, the lateral wall of box corresponds operating tube punishment does not is equipped with logical groove, the operating tube passes respectively logical groove, the inside wall that leads to the groove is equipped with the rectangular channel, the inside wall of rectangular channel slides respectively and is equipped with the rectangular plate, the both sides surface of rectangular plate respectively with the internal surface of rectangular channel contacts, the operating tube respectively with correspond rectangular plate fixed connection.
Preferably, the outer surface of the culture bottle is fixedly connected with a connecting plate, the upper side wall of the box body is screwed with an adjusting screw rod, one end of the adjusting screw rod is rotatably connected with the connecting plate, and the other end of the adjusting screw rod penetrates through the upper side wall of the box body and is fixedly connected with an adjusting handle.
Preferably, the upper side wall of the box body is provided with a through hole, and the upper end of the culture bottle penetrates through the through hole.
Preferably, a sealing ring is arranged between the through hole and the culture bottle.
Preferably, the liquid storage device further comprises a liquid storage bottle, the lower end of the liquid storage bottle is connected with one end of a guide pipe, the other end of the guide pipe penetrates through the side wall of the box body, and a third valve is arranged on the guide pipe.
Compared with the prior art, the beneficial effects of the utility model are that: the utility model provides a tissue cell culture bottle, through setting up the box, at the inside blake bottle that sets up of box, place the inside at the box with the blake bottle, and place the tissue cell in the blake bottle, thereby effect through osmotic pressure, the inside culture solution of box can infiltrate in the blake bottle, the tissue cell to in the blake bottle cultivates, when changing the blake liquid, only need pass through the drain pipe that sets up on the box, can with the play of culture solution in the box, and when needing to add the blake liquid, can annotate the blake liquid through the feed liquor pipe, through setting up the control tube, make the control tube run through box lateral wall and blake bottle intercommunication, thereby both hands can infiltrate in the control tube, operate the tissue cell in the blake bottle, high durability and convenient use and simple structure.
Drawings
Fig. 1 is a schematic front view, sectional structure of embodiment 1 of the present invention;
fig. 2 is a schematic top view of embodiment 1 of the present invention;
fig. 3 is a schematic left-side sectional structure view according to embodiment 1 of the present invention;
fig. 4 is a schematic front view of a cross-sectional structure according to embodiment 2 of the present invention;
fig. 5 is a left side view structural diagram of the case body according to embodiment 2 of the present invention.
In the figure: 1-box body, 2-culture bottle, 3-operation tube, 4-first sealing cover, 5-convex hook, 6-second sealing cover, 7-liquid discharge tube, 8-first valve, 9-liquid inlet tube, 10-second valve, 11-through groove, 12-rectangular groove, 13-rectangular plate, 14-connecting plate, 15-adjusting screw rod, 16-adjusting handle, 17-liquid storage bottle, 18-conduit and 19-third valve.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Example 1
Referring to fig. 1-3, the present invention provides a technical solution: a tissue cell culture bottle comprises a box body 1, as shown in figure 2, the box body 1 is cylindrical and is convenient to place, the box body 1 is circular, internal dead angles are reduced, and cleaning is convenient, a culture bottle 2 is arranged in the box body 1, the culture bottle 2 is cylindrical, the culture bottle 2 and the box body 1 are coaxially arranged, the culture bottle 2 is made of hollow fiber materials, the materials allow proteins, amino acids and the like of macromolecules in a culture solution to permeate through, but not allow tissue cells to permeate through, so that the tissue cells in the culture bottle 2 cannot run out of the culture bottle 2, an operation tube 3 is symmetrically arranged on the side wall of the culture bottle 2, the operation tubes 3 are all cylindrical, arms of users can be stretched into the operation tube 3 to operate the tissue cells in the prime number culture bottle 2, one end of the operation tube 3 respectively runs through the side wall of the culture bottle 2, the other end of the operation tube 3 respectively penetrates through the side wall of the box body 1, the side wall of the other end of the operation tube 3 is respectively hinged with a first sealing cover 4, one end of the first sealing cover 4 is hinged with the side wall of the operation tube 3, the outer surface of the operation tube 3, far away from the corresponding hinged position of the first sealing cover 4, is respectively hinged with a convex hook 5, the convex hook 5 is matched with the first sealing cover 4, namely the convex hook 5 can hook the other end of the sealing cover 4, so that the sealing cover 4 can seal the operation tube 3 to prevent external pollutants from entering from the book operation tube 3, the upper end of the culture bottle 2 is screwed with a second sealing cover 6, the lower end of the side wall of the box body 1 is provided with one end of a liquid discharge tube 7, the liquid discharge tube 7 is provided with a first valve 8, and the first valve 8 can adopt conventional valve equipment on the market, play to the opening and shutting of fluid-discharge tube 7 to when opening first valve 8, the culture solution of box 1 inside is discharged by fluid-discharge tube 7, the lateral wall upper end of box 1 is equipped with feed liquor pipe 9, be equipped with second valve 10 on the feed liquor pipe 9, by feed liquor pipe 9 to the inside new culture solution that pours into of box 1.
Specifically, as shown in fig. 1, the operation tubes 3 are all disposed obliquely, and one end of the operation tube 3 away from the culture bottle 2 is higher than one end of the operation tube 3 close to the culture bottle 2, so that the operation tube is convenient for a user to use, and simultaneously prevents the liquid in the culture bottle 2 from flowing out from the operation tube 3.
Specifically, as shown in fig. 3, the culture medium container further comprises a liquid storage bottle 17, one end of a conduit 18 is connected to the lower end of the liquid storage bottle 17, the other end of the conduit 18 penetrates through the side wall of the container body 1, a third valve 19 is arranged on the conduit 18, a new culture medium is stored in the liquid storage bottle 17, the third valve 19 is opened, and the culture medium in the liquid storage bottle 17 enters the container body 1 through the conduit 18, so that the culture medium can be supplemented at any time.
The working principle is as follows: the utility model discloses during the use, add histiocyte in blake bottle 2, open second valve 10, add the culture solution to box 1 through feed liquor pipe 9, make the culture solution submerge blake bottle 2, carry out the histiocyte culture in blake bottle 2, then, when cultivateing a period and need changing the culture solution, only need open first valve 8, make the culture solution by fluid-discharge tube 7 discharge can, add new culture solution again by feed liquor pipe 9, when needs go on further operation to cell tissue, open protruding hook 5, make first sealed lid 4 open, both hands are stretched into by control tube 3 and can be operated histiocyte in the blake bottle.
Example 2
Referring to fig. 4-5, the present embodiment is different from embodiment 1 in that: through grooves 11 are respectively arranged on the side wall of the box body 1 corresponding to the operating tubes 3, the operating tubes 3 respectively penetrate through the through grooves 11, as shown in fig. 5, the operating tubes 3 can slide up and down along the through grooves 11, rectangular grooves 12 are arranged on the inner side walls of the through grooves 11, rectangular plates 13 are respectively arranged on the inner side walls of the rectangular grooves 12 in a sliding manner, the surfaces of the two sides of each rectangular plate 13 are respectively contacted with the inner surfaces of the rectangular grooves 12, the through grooves 11 can be always blocked when the rectangular plates 13 slide up and down, so that the sealing inside the box body 1 is kept, the operating tubes 3 are respectively fixedly connected with the corresponding rectangular plates 13, the position of the culture bottle 2 in the box body 1 can be changed by controlling the operating tubes 3, so that the culture bottle 2 can move upwards when tissue cells need to be operated, and the culture bottle 2 is separated from culture solution, thereby eliminating the need to drain the culture solution.
Particularly, the outer fixed surface of blake bottle 2 is connected with connecting plate 14, the last lateral wall spiro union of box 1 has adjusting screw 15, adjusting screw 15 one end with connecting plate 14 rotates to be connected, adjusting screw 15's the other end passes the last lateral wall fixedly connected with adjustment handle 16 of box 1 rotates adjustment handle 16, can control blake bottle 2 reciprocates, is convenient for control blake bottle 2's position.
Specifically speaking, the upper side wall of box 1 is equipped with the through-hole, the upper end of blake bottle 2 is passed the through-hole is convenient for take out the inside histocyte of blake bottle 2.
Specifically, a seal ring is provided between the through hole and the culture bottle 2, so that a gap between the through hole and the culture bottle 2 is kept sealed.
In the description of the present invention, unless expressly stated or limited otherwise, the terms "mounted," "connected," and "fixed" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral part; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The utility model discloses the standard part that uses all can purchase from the market, and dysmorphism piece all can be customized according to the description with the record of drawing of description, and the concrete connection mode of each part all adopts conventional means such as ripe bolt, rivet, welding among the prior art, and machinery, part and equipment all adopt prior art, and conventional model, including circuit connection adopts conventional connection mode among the prior art, does not detailed here again.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A kind of tissue cell culture bottle, including the container body (1), its characterized in that: the box body (1) is cylindrical, a culture bottle (2) is arranged inside the box body (1), the culture bottle (2) is cylindrical, the culture bottle (2) and the box body (1) are coaxially arranged, an operating pipe (3) is symmetrically arranged on the side wall of the culture bottle (2), one end of the operating pipe (3) penetrates through the side wall of the culture bottle (2) respectively, the other end of the operating pipe (3) penetrates through the side wall of the box body (1) respectively, the other end of the operating pipe (3) is hinged with a first sealing cover (4) respectively, a protruding hook (5) is hinged at the position, far away from the corresponding first sealing cover (4), of the outer surface of the operating pipe (3), the protruding hook (5) is matched with the first sealing cover (4), a second sealing cover (6) is screwed at the upper end of the culture bottle (2), the liquid discharging device is characterized in that one end of a liquid discharging pipe (7) is arranged at the lower end of the side wall of the box body (1), a first valve (8) is arranged on the liquid discharging pipe (7), a liquid inlet pipe (9) is arranged at the upper end of the side wall of the box body (1), and a second valve (10) is arranged on the liquid inlet pipe (9).
2. A tissue culture flask according to claim 1, wherein: the operating tubes (3) are all obliquely arranged, and one ends of the operating tubes (3) far away from the culture bottles (2) are higher than one ends of the operating tubes (3) close to the culture bottles (2).
3. A tissue culture flask according to claim 2, wherein: the lateral wall of box (1) corresponds operating tube (3) punishment do not is equipped with logical groove (11), operating tube (3) pass respectively logical groove (11), the inside wall that leads to groove (11) is equipped with rectangular channel (12), the inside wall of rectangular channel (12) slides respectively and is equipped with rectangular plate (13), the both sides surface of rectangular plate (13) respectively with the internal surface of rectangular channel (12) contacts, operating tube (3) respectively with correspond rectangular plate (13) fixed connection.
4. A tissue culture flask according to claim 3, wherein: the outer fixed surface of blake bottle (2) is connected with connecting plate (14), the last lateral wall spiro union of box (1) has adjusting screw (15), adjusting screw (15) one end with connecting plate (14) rotate to be connected, adjusting screw (15) the other end passes the last lateral wall fixedly connected with adjustment handle (16) of box (1).
5. A tissue culture flask according to claim 4, wherein: the upper side wall of the box body (1) is provided with a through hole, and the upper end of the culture bottle (2) penetrates through the through hole.
6. A tissue culture flask according to claim 5, wherein: a sealing ring is arranged between the through hole and the culture bottle (2).
7. A tissue culture flask according to claim 1, wherein: still including stock solution bottle (17), the lower extreme of stock solution bottle (17) is connected with the one end of pipe (18), the other end of pipe (18) runs through the lateral wall of box (1), be equipped with third valve (19) on pipe (18).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020536151.XU CN213570526U (en) | 2020-04-13 | 2020-04-13 | Tissue cell culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020536151.XU CN213570526U (en) | 2020-04-13 | 2020-04-13 | Tissue cell culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213570526U true CN213570526U (en) | 2021-06-29 |
Family
ID=76520961
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020536151.XU Expired - Fee Related CN213570526U (en) | 2020-04-13 | 2020-04-13 | Tissue cell culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213570526U (en) |
-
2020
- 2020-04-13 CN CN202020536151.XU patent/CN213570526U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210629 |