CN213567218U

CN213567218U - Epithelial and epithelial-mesenchymal transition type circulating tumor cell detection kit

Info

Publication number: CN213567218U
Application number: CN202022478824.3U
Authority: CN
Inventors: 张开山; 饶浪晴
Original assignee: HANGZHOU WATSON BIOTECH Inc
Current assignee: HANGZHOU WATSON BIOTECH Inc
Priority date: 2020-10-30
Filing date: 2020-10-30
Publication date: 2021-06-29
Anticipated expiration: 2030-10-30

Abstract

The application relates to the field of detection devices, in particular to an epithelial and epithelial-mesenchymal transition type circulating tumor cell detection kit which is reasonable in design and combination, can be directly used for storage and transportation in a normal-temperature environment, can be used for cold chain transportation and storage of a low-temperature preservation reagent, ensures the validity of reagent consumable combination in the kit, is convenient for checking the name of a medicine reagent and improves the using effect; the utility model discloses a box body, the lid, the baffle, the test-tube rack, the dish absorbs water, the otter board, ice bag group and fixing base, the lid is established at the box body top, the box body top is equipped with the mounting groove, the baffle is fixed on the mounting groove inside wall, the dish that absorbs water is placed on the mounting groove diapire, the otter board is divided and is established in baffle bottom both sides, ice bag group is put between otter board and mounting groove lateral wall, the test-tube rack pull is fixed between the otter board of both sides, the fixing base is located the baffle top, the fixing base top is equipped with material standing groove and a plurality of medicine standing groove, and all be equipped with the nameplate.

Description

Epithelial and epithelial-mesenchymal transition type circulating tumor cell detection kit

Technical Field

The application relates to the field of detection devices, in particular to an epithelial and epithelial-mesenchymal transition type circulating tumor cell detection kit.

Background

Circulating Tumor Cells (CTCs) refer to Tumor Cells released from solid tumors or metastases into the circulation of peripheral blood spontaneously or due to medical procedures, and are a general term for various Tumor Cells existing in peripheral blood, and CTCs are heterogeneous populations mainly composed of epithelial CTCs, epithelial-mesenchymal mixed CTCs, and mesenchymal CTCs subsets.

Need oneself to prepare experimental medicine before circulating tumor cell detects, will spend a lot of time, still need classify the medicine after the experiment is accomplished, if lack one of them medicine, still need look for other medicine and remove the substitution, it does not have much but to cause the time of really using in the experiment at last, consequently multipurpose tumor cell detect reagent box sums up the detection medicine bottle and holds now, but the most temperature to the inside of kit that can not keep warm for a long time of tumor cell detect reagent box on the existing market, the inconvenient cryopreservation reagent to the preparation transports and stores, and the medicine bottle front side is write the fixed knot who has the name and is constructed by the kit in most and shelters from, inconvenient looking over medicine reagent name, there may exist the misuse phenomenon, influence the result of use.

SUMMERY OF THE UTILITY MODEL

Based on this, this application provides a reasonable, both can directly be used for normal atmospheric temperature environment's preservation and transportation of design combination, can carry out the cold chain transportation to the low-temperature preservation reagent again and store, has ensured the reagent consumptive material combination validity in the kit, conveniently looks over medicine reagent name simultaneously, reduces medicine reagent misuse condition in the operation process, improves the epithelia and the epithelial mesenchymal transition type circulation tumor cell detection kit of result of use.

The application provides an epithelial and epithelial-mesenchymal transition type circulating tumor cell detection kit, which comprises a box body, a box cover, a clapboard, a test tube rack, a water absorption disc, a screen plate, an ice bag group and a fixed seat, the box cover is arranged at the top of the box body, the top of the box body is provided with a placing groove, the clapboard is fixed on the inner side wall of the placing groove, the front side of the box body is provided with a taking and placing opening, a stop door is arranged at the taking and placing opening, the water absorption disc is placed on the bottom wall in the placing groove, the screen plates are respectively arranged at two sides of the bottom of the partition plate, the ice bag components are arranged between the screen plates and the side walls of the placing groove, the test tube rack is fixed between the screen plates on the two sides in a pulling way, the test tube rack is provided with a placing seat, the fixed seat is positioned above the clapboard, the top of the fixed seat is provided with a material placing groove and a plurality of medicine placing grooves, and a name plate block is arranged beside each medicine placing groove, a reagent bottle is placed in each medicine placing groove, and the name of the medicine in the corresponding reagent bottle is marked on each name plate block.

Preferably, the name plate block is made of transparent materials, a slot is formed in one side of the name plate block, a paper sheet is inserted into the slot, and names of medicines in the corresponding reagent bottles beside the name plate block are written on the paper sheet.

Preferably, the water absorption plate comprises a placing plate and a water absorption sponge cushion, the outer side wall of the placing plate is in contact with the inner side wall of the placing groove, a sponge placing groove is formed in the top of the placing plate, the water absorption sponge cushion is fixed inside the sponge placing groove, and the test tube rack and the ice bag set are located above the water absorption sponge cushion.

Preferably, the test tube rack comprises a support plate, a bottom plate and two connecting plates, sliding rails are arranged on the screen plates on two sides of the bottom of the partition plate, the connecting plates are connected with output ends of the sliding rails, two sides of the top of the bottom plate are respectively connected with the bottoms of the two connecting plates, two sides of the support plate are respectively connected with the middle parts of the two connecting plates, and the support plate is provided with a plurality of fixing holes.

Preferably, the placing seat is fixed on the upper side face of the bottom plate, the placing seat is a high-density sponge block, a plurality of refrigerant placing grooves are formed in the top of the placing seat, and the refrigerant placing grooves are located under the fixing holes.

Preferably, the fixing base selects the high-density sponge block, the high-density sponge block is provided with a plurality of nameplate grooves, and the bottom of the nameplate block is inserted into the nameplate grooves.

Preferably, a plurality of spiral sample chambers and microfluidic chips are placed in the material placement groove.

Preferably, the inner side wall of the box body is provided with aluminum foil paper.

Preferably, the rear side of the box cover is hinged with the rear side of the box body through a hinge, and the front sides of the box cover and the box body are provided with lock catches.

Preferably, the inner top wall of the box cover is provided with a sponge plate.

Compared with the prior art, the utility model has the following characteristics and beneficial effect:

1. when the reagent carries out normal atmospheric temperature environment's save and transportation in needs, only need with the splendid attire have experimental reagent the medicine bottle place on the fixing base can, if still need carry out cold chain transportation to the cryogenically preserved reagent and store, then put frozen ice bag earlier between otter board and resettlement groove lateral wall, later take out the test-tube rack from the box body, then place the medicine bottle that has the cryogenically preserved reagent in placing the seat, inside pushing back the test-tube rack box body after putting, the cold volume that gives off through the ice bag carries out the cryogenically preserved to the reagent, and absorb the drop that the ice bag outer wall dropped through the dish that absorbs water in the save process, and the use effect is improved.

2. The name plate block on the fixing seat is used for marking and displaying the name of the medicine, so that the name of the medicine reagent is conveniently checked, the misuse condition of the medicine reagent in the operation process is reduced, and the use effect is improved.

Drawings

In order to more clearly illustrate the embodiments of the present application or technical solutions in related arts, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without inventive efforts.

Fig. 1 is a schematic structural view of the present invention;

FIG. 2 is a top view of FIG. 1;

fig. 3 is an external schematic view of the present invention;

fig. 4 is a structural diagram of a nameplate block.

Reference numerals: 1. a box body; 2. a box cover; 3. a partition plate; 4. a test tube rack; 5. a water absorbing pan; 6. a screen plate; 7. a set of ice bags; 8. a fixed seat; 9. a shutter; 10. a material placement groove; 11. a medicine placing groove; 12. a name card block; 13. a reagent bottle; 14. a sheet of paper; 15. placing a tray; 16. a water-absorbing sponge cushion; 17. a support plate; 18. a base plate; 19. a connecting plate; 20. a placing seat; 21. a spiral sample chamber; 22. a microfluidic chip; 23. aluminum foil paper; 24. locking; 25. a sponge plate; 26. a cryogen placing groove; 27. test tubes.

Detailed Description

In order to make the objects, technical solutions and advantages of the present application more apparent, the present application is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application. All other examples, which can be obtained by a person skilled in the art without making any inventive step based on the examples in this application, are within the scope of protection of this application.

It should be understood by those skilled in the art that technical or scientific terms used in the claims and the specification should have the ordinary meaning as understood by those having ordinary skill in the art to which this application belongs. "connected" or "coupled" and like terms are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect; the terms "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like refer to an orientation or positional relationship as shown in the drawings, which are used for convenience in describing the invention and to simplify the description, and do not indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore, the above-described terms are not to be construed as limiting the invention.

Referring to fig. 1 to 4, in the embodiment of the present application, an epithelial-type and epithelial-mesenchymal transition-type circulating tumor cell detection kit is provided, which includes a box body 1, a box cover 2, a partition plate 3, a test tube rack 4, a water absorption tray 5, a mesh plate 6, an ice bag set 7 and a fixing base 8, wherein the box cover 2 is disposed at the top of the box body 1, the box body 1 is provided with a placement groove at the top thereof, the partition plate 3 is fixed on the inner side wall of the placement groove, the box body 1 is provided with a pick-and-place opening at the front side thereof, a door 9 is disposed at the pick-and-place opening, the water absorption tray 5 is disposed on the inner bottom wall of the placement groove, beads falling from the outer wall of the ice bag are absorbed by the water absorption tray 5 during the storage process, the mesh plates 6 are disposed at both sides of the bottom of the partition plate 3, the ice bag set 7 is disposed between the mesh plate 6 and the side wall of the placement, fixing base 8 is located 3 tops of baffle, 8 tops of fixing base are equipped with material standing groove 10 and a plurality of medicine standing groove 11, and all be equipped with name tablet piece 12 at 11 sides of every medicine standing groove, reagent bottle 13 has been placed in the medicine standing groove 11, all mark the name of medicine in the corresponding reagent bottle 13 on every name tablet piece 12, show through name tablet piece 12 mark to the name of medicine, conveniently look over medicine reagent name, reduce the medicine reagent misuse condition in the operation process, and the use effect is improved.

Preferably, the name card block 12 is made of transparent materials, a slot is formed in one side of the name card block 12, a paper sheet 14 is inserted into the slot, the name of the medicine in the corresponding reagent bottle 13 beside the name card block 12 is written on the paper sheet 14, and the name of the medicine record is changed at will by replacing the paper sheet 14 conveniently.

Preferably, the water absorption plate 5 comprises a placing plate 15 and a water absorption foam-rubber cushion 16, the outer side wall of the placing plate 15 is in contact with the inner side wall of the placing groove, a sponge placing groove is formed in the top of the placing plate 15, the water absorption foam-rubber cushion 16 is fixed inside the sponge placing groove, the test tube rack 4 and the ice bag group 7 are both located above the water absorption foam-rubber cushion 16, and water flowing out of the ice bag can be adsorbed through the water absorption foam-rubber cushion 16.

Preferably, test-tube rack 4 includes extension board 17, bottom plate 18 and two connecting plates 19, all is equipped with the slide rail on being located the otter board 6 of 3 bottom both sides of baffle, and connecting plate 19 is connected with the slide rail output, and 18 top both sides of bottom plate are connected with two connecting plates 19 bottoms respectively, and extension board 17 both sides are connected with 19 middle parts of two connecting plates respectively, are equipped with a plurality of fixed orificess on the extension board 17, and the fixed orifices through on the extension board 17 carries on spacingly to the test tube, supports the test tube through bottom plate 18.

Preferably, the placing seat 20 is fixed on the upper side surface of the bottom plate 18, the placing seat 20 is made of a high-density sponge block, a plurality of refrigerant placing grooves 26 are formed in the top of the placing seat 20, and the refrigerant placing grooves 26 are located right below the fixing holes.

Preferably, the fixing base 8 selects the high-density sponge block for use, is equipped with a plurality of nameplate grooves on the high-density sponge block, and the fixed effect to reagent bottle 13 is guaranteed through the compliance of high-density sponge block to insert in the nameplate groove at nameplate 12 bottoms.

Preferably, the material placing groove 10 is placed with a plurality of spiral sample chambers 21 and microfluidic chips 22, and the spiral sample chambers 21 and microfluidic chips 22 for auxiliary detection are accommodated therein.

Preferably, the inner side wall of the box body 1 is provided with aluminum foil paper 23, and the heat preservation performance of the box body 1 is enhanced through the heat preservation performance of the aluminum foil paper 23.

Preferably, the back side of the box cover 2 is hinged with the back side of the box body 1 through a hinge, and a lock catch 24 is arranged at the front sides of the box cover 2 and the box body 1.

Preferably, the top wall is provided with a sponge plate 25 in the box cover 2, and the stability of the reagent bottle 13 on the fixing seat 8 is enhanced through the flexibility of the sponge plate.

To sum up, in the kit for detecting epithelial-type and epithelial-mesenchymal transition-type circulating tumor cells provided in the embodiment of the present application, when in use, the reagent bottle 13 is fixed by the medicine placing groove 11 on the fixing base 8, the spiral sample chamber 21 and the microfluidic chip 22 are stored by the material placing groove 10, the name of the medicine is marked and displayed by the nameplate block 12 on the fixing base 8, the name of the medicine and the reagent is conveniently checked, the misuse condition of the medicine and the reagent in the operation process is reduced, if the name of the nameplate block 12 is to be changed, the nameplate block 12 can be taken out from the fixing base 8, the paper 14 can be taken out from the nameplate block 12, the paper 14 written with a new name can be inserted into the nameplate block 12, the nameplate block 12 can be put back to the original position, the test tube 27 can be inserted into the test tube rack 4 to store the test tube 27, so that the test tube rack 4 can be directly pulled out from the box body 1 to use the test tube 27 during detection, the test tube 27 is convenient to take and use, and the experiment operation is simpler and more convenient; when carrying out cold chain transportation to needs cryopreserving reagent and storing, then put freezing good ice bag group 7 between otter board 6 and arrangement groove lateral wall earlier, later take out test-tube rack 4 from box body 1, then place the medicine bottle that contains the cryopreserving reagent in the cryogen standing groove 26 of placing the seat, push back test-tube rack 4 box body 1 portion after putting, cold volume through ice bag group 7 gived off carries out the cryopreservation to reagent, and absorb the drop of water that the ice bag outer wall dropped through water absorption tray 5 at the preservation in-process, and the use effect is improved.

The technical features of the above embodiments can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the above embodiments are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present application, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present application. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the concept of the present application, which falls within the scope of protection of the present application. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. The epithelial and epithelial-mesenchymal transition type circulating tumor cell detection kit is characterized by comprising a box body (1), a box cover (2), a partition plate (3), a test tube rack (4), a water absorption disc (5), a screen plate (6), an ice bag group (7) and a fixing seat (8), wherein the box cover (2) is arranged at the top of the box body (1), a placing groove is formed in the top of the box body (1), the partition plate (3) is fixed on the inner side wall of the placing groove, a taking and placing opening is formed in the front side of the box body (1), a blocking door (9) is arranged at the taking and placing opening, the water absorption disc (5) is placed on the inner bottom wall of the placing groove, the screen plate (6) is respectively arranged at two sides of the bottom of the partition plate (3), the ice bag group (7) is respectively placed between the screen plate (6) and the side wall of the placing groove, the test tube rack (4) is fixed between the screen plates (6) at two sides, a placing seat (20, fixing base (8) top is equipped with material standing groove (10) and a plurality of medicine standing groove (11) to all be equipped with brand piece (12) at every medicine standing groove (11) side, reagent bottle (13) have been placed in medicine standing groove (11), all be marked with the name of medicine in corresponding reagent bottle (13) on every brand piece (12).

2. The kit for detecting epithelial and epithelial-mesenchymal transition-type circulating tumor cells according to claim 1, wherein the nameplate block (12) is made of a transparent material, a slot is formed in one side of the nameplate block (12), a paper sheet (14) is inserted into the slot, and names of drugs in corresponding reagent bottles (13) beside the nameplate block (12) are written on the paper sheet (14).

3. The kit for detecting epithelial-type and epithelial-mesenchymal transition-type circulating tumor cells according to claim 1, wherein the water absorption tray (5) comprises a placing tray (15) and a water absorption sponge mat (16), the outer side wall of the placing tray (15) is in contact with the inner side wall of the placing groove, a sponge placing groove is formed in the top of the placing tray (15), the water absorption sponge mat (16) is fixed inside the sponge placing groove, and the test tube rack (4) and the ice bag set (7) are both positioned above the water absorption sponge mat (16).

4. The kit for detecting epithelial-type and epithelial-mesenchymal transition-type circulating tumor cells according to claim 1, wherein the test tube rack (4) comprises a support plate (17), a bottom plate (18) and two connecting plates (19), slide rails are arranged on the mesh plate (6) at two sides of the bottom of the partition plate (3), the connecting plates (19) are connected with the output ends of the slide rails, two sides of the top of the bottom plate (18) are respectively connected with the bottoms of the two connecting plates (19), two sides of the support plate (17) are respectively connected with the middle parts of the two connecting plates (19), and a plurality of fixing holes are arranged on the support plate (17).

5. The kit for detecting epithelial-type and epithelial-mesenchymal transition-type circulating tumor cells according to claim 4, wherein the holder (20) is fixed on the upper side of the bottom plate (18), the holder (20) is made of a high-density sponge block, a plurality of refrigerant holding grooves (26) are formed in the top of the holder (20), and the refrigerant holding grooves (26) are located right below the fixing holes.

6. The kit for detecting epithelial-type and epithelial-mesenchymal transition-type circulating tumor cells according to claim 1, wherein the fixing base (8) is a high-density sponge block, the high-density sponge block is provided with a plurality of nameplate grooves, and the bottoms of the nameplate blocks (12) are inserted into the nameplate grooves.

7. The kit for detecting epithelial-to-mesenchymal transition-type circulating tumor cells according to claim 1, wherein a plurality of spiral sample chambers (21) and microfluidic chips (22) are disposed in the material placement tank (10).

8. The kit for detecting epithelial-to-epithelial-mesenchymal transition-type circulating tumor cells according to claim 1, wherein the box body (1) is provided on its inner sidewall with aluminum foil paper (23).

9. The kit for detecting epithelial-type and epithelial-mesenchymal transition-type circulating tumor cells according to claim 1, wherein the rear side of the box cover (2) is hinged to the rear side of the box body (1) through a hinge, and a lock catch (24) is arranged on the front sides of the box cover (2) and the box body (1).

10. The kit for detecting epithelial-to-epithelial-mesenchymal transition-type circulating tumor cells according to claim 1, wherein the inner top wall of the box cover (2) is provided with a sponge plate (25).