CN213537951U - CHO cell serum-free medium - Google Patents

Abstract

The utility model discloses a CHO cell serum-free medium, including the culture dish that is equipped with CHO cell serum-free culture medium, culture dish bottom central authorities have embedded the motor, the power output shaft of motor extends to in the culture dish, the bottom is located the power output shaft outside and installs the bearing in the culture dish, the bearing housing is established in the bracing piece bottom, power output shaft and bracing piece bottom rigid coupling, bracing piece upper portion has seted up the multichannel spout along bracing piece length direction, the cover is equipped with the sleeve on the bracing piece, the sleeve inner wall has set firmly a plurality of sliders that match with the spout, the sleeve side has set firmly many puddlers. The utility model discloses a combine motor and culture dish, drive the bracing piece through starter motor and rotate to drive the puddler and rotate the serum-free culture medium of CHO cell in the stirring culture dish, do not need external agitated vessel to stir the culture medium.

Description

CHO cell serum-free medium

Technical Field

The utility model relates to a culture medium field especially relates to a CHO cell serum-free medium.

Background

The culture medium is a nutrient medium prepared from different nutrient substances for the growth and reproduction of microorganisms, plants or animals (or tissues). Generally comprises several major substances such as carbohydrate, nitrogen-containing substances, inorganic salts (including trace elements), vitamins and water. The culture medium is not only a basic substance for providing nutrition for the cells and promoting the proliferation of the cells, but also a living environment for the growth and the propagation of the cells. The culture medium has many kinds, and can be divided into natural culture medium, synthetic culture medium and semi-synthetic culture medium according to the source of the preparation raw material; can be divided into solid culture medium, liquid culture medium and semisolid culture medium according to physical state; according to the culture function, the culture medium can be divided into a basic culture medium, a selective culture medium, an enriched culture medium, an identification culture medium and the like; according to the application range, the culture medium can be divided into a bacteria culture medium, an actinomycete culture medium, a yeast culture medium, a fungus culture medium and the like. The media is typically tested and pH adjusted after preparation, and sterilized, typically by autoclaving and filtration. The culture medium is rich in nutrients and is easy to be polluted or deteriorated. It should not be placed for a long time after being prepared, and is best to be prepared for use immediately.

At present, the CHO cell serum-free culture medium is easy to precipitate and stratify after being stored for a long time, stirring is needed before use, stirring equipment is usually needed, extra carrying is needed, the operation process is complex, and improvement is needed.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a CHO cell serum-free culture medium to solve the technical problem.

The utility model discloses a solve above-mentioned technical problem, adopt following technical scheme to realize:

the utility model provides a CHO cell serum-free culture medium, is including the culture dish that is equipped with CHO cell serum-free culture medium, the embedded motor that has in culture dish bottom central authorities, the power output shaft of motor extends to in the culture dish, the bottom is located the power output shaft outside and installs the bearing in the culture dish, the bearing housing is established in the bracing piece bottom, power output shaft and bracing piece bottom rigid coupling, bracing piece upper portion has seted up the multichannel spout along bracing piece length direction, the cover is equipped with the sleeve on the bracing piece, the sleeve inner wall has set firmly a plurality of sliders that match with the spout, the sleeve side has set firmly many puddlers.

Preferably, a cover plate is arranged above the culture dish, a rotary drum is fixedly arranged in the center of the bottom surface of the cover plate, and the rotary drum is matched with the top end of the support rod in an inserted manner.

Preferably, a motor controller is fixedly arranged on the top surface of the cover plate and is in wireless connection with the motor.

Preferably, a liquid adding pipe penetrates and is fixedly arranged on the cover plate, and a sealing cap is connected to the top end of the liquid adding pipe in a threaded mode.

Preferably, the culture dish and the cover plate are made of glass.

Preferably, the stirring rod is made of plastic.

Preferably, the sleeve is made of stainless steel.

The utility model has the advantages that:

1. the utility model discloses a combine motor and culture dish, drive the bracing piece through starter motor and rotate to drive the puddler and rotate the serum-free culture medium of CHO cell in the stirring culture dish, do not need external agitated vessel to stir the culture medium.

2. The culture dish can be sealed through the cover plate, the CHO cell serum-free culture medium is prevented from spilling out during stirring, and an additional preparation can be added into the culture dish during stirring through the liquid feeding pipe.

Drawings

Fig. 1 is a schematic structural view of the present invention;

fig. 2 is a schematic top view of the sleeve of the present invention;

reference numerals: 1-sealing cap; 2-a liquid adding pipe; 3-a rotating drum; 5-a motor controller; 6-cover plate; 7-a stirring rod; 8-a support bar; 9-a chute; 10-a sleeve; 11-a bearing; 12-a power take-off shaft; 13-a motor; 14-a culture dish; 15-a slide block; 16-CHO cell serum-free culture medium.

Detailed Description

In order to make the technical means, the creation features, the achievement purposes and the functions of the present invention easy to understand, the present invention will be further explained below with reference to the following embodiments and the accompanying drawings, but the following embodiments are only the preferred embodiments of the present invention, and not all embodiments are included. Based on the embodiments in the implementation, other embodiments obtained by those skilled in the art without any creative work belong to the protection scope of the present invention.

Specific embodiments of the present invention will be described below with reference to the accompanying drawings.

As shown in fig. 1-2, a CHO cell serum-free medium comprises a culture dish 14 containing a CHO cell serum-free medium 16, a motor 13 is embedded in the center of the bottom of the culture dish 14, a power output shaft 12 of the motor 13 extends into the culture dish 14, a bearing 11 is installed on the outer side of the power output shaft 12 at the bottom of the culture dish 14, the bearing 11 is sleeved on the bottom of a support rod 8, the power output shaft 12 is fixedly connected with the bottom end of the support rod 8, a plurality of sliding grooves 9 are formed in the upper part of the support rod 8 along the length direction of the support rod 8, a sleeve 10 is sleeved on the support rod 8, the sleeve 10 is made of stainless steel, a plurality of sliding blocks 15 matched with the sliding grooves 9 are fixedly arranged on the inner wall of the sleeve 10, a plurality of stirring rods 7 are fixedly arranged on the.

A cover plate 6 is arranged above the culture dish 14, a rotary drum 3 is fixedly arranged in the center of the bottom surface of the cover plate 6, and the rotary drum 3 is matched with the top end of the support rod 8 in an inserting mode.

The top surface of the cover plate 6 is fixedly provided with a motor controller 5, the motor controller 5 is wirelessly connected with a motor 13, the cover plate 6 is fixedly provided with a liquid feeding pipe 2 in a penetrating way, and the top end of the liquid feeding pipe 2 is in threaded connection with a sealing cap 1.

The working principle is as follows: when the CHO cell serum-free culture medium 16 in the culture dish 14 needs to be stirred, the supporting rod 8 is driven to rotate through the starting motor 13, so that the stirring rod 7 is driven to rotate and stir the CHO cell serum-free culture medium 16 in the culture dish 14, the culture medium can be stirred without external stirring equipment, the culture dish 14 can be sealed through the cover plate 6, the CHO cell serum-free culture medium 16 is prevented from spilling out during stirring, and an additional preparation can be added into the culture dish 14 through the liquid feeding pipe 2 during stirring.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It should be understood by those skilled in the art that the present invention is not limited by the above embodiments, and the description in the above embodiments and the description is only preferred examples of the present invention, and is not intended to limit the present invention, and that the present invention can have various changes and modifications without departing from the spirit and scope of the present invention, and these changes and modifications all fall into the scope of the claimed invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (7)

1. A CHO cell serum-free culture medium comprising a culture dish (14) filled with a CHO cell serum-free culture medium (16), characterized in that: culture dish (14) bottom central authorities are embedded to have motor (13), power output shaft (12) of motor (13) extend to in culture dish (14), bottom is located power output shaft (12) outside and installs bearing (11) in culture dish (14), bearing (11) cover is established in bracing piece (8) bottom, power output shaft (12) and bracing piece (8) bottom rigid coupling, bracing piece (8) upper portion has seted up multichannel spout (9) along bracing piece (8) length direction, the cover is equipped with sleeve (10) on bracing piece (8), sleeve (10) inner wall has set firmly slider (15) a plurality of and spout (9) matching, sleeve (10) side has set firmly many puddler (7).

2. The CHO cell serum-free medium according to claim 1, wherein: a cover plate (6) is arranged above the culture dish (14), a rotary drum (3) is fixedly arranged in the center of the bottom surface of the cover plate (6), and the rotary drum (3) is matched with the top end of the support rod (8) in an inserting manner.

3. A CHO cell serum-free medium according to claim 2, wherein: the top surface of the cover plate (6) is fixedly provided with a motor controller (5), and the motor controller (5) is wirelessly connected with a motor (13).

4. A CHO cell serum-free medium according to claim 3, characterized in that: the liquid adding pipe (2) penetrates and is fixedly arranged on the cover plate (6), and the top end of the liquid adding pipe (2) is in threaded connection with a sealing cap (1).

5. A CHO cell serum-free medium according to claim 2, wherein: the culture dish (14) and the cover plate (6) are made of glass.

6. The CHO cell serum-free medium according to claim 1, wherein: the stirring rod (7) is made of plastic.

7. The CHO cell serum-free medium according to claim 1, wherein: the sleeve (10) is made of stainless steel.

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