CN213407902U - Filtering column for nucleic acid extractor - Google Patents
Filtering column for nucleic acid extractor Download PDFInfo
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- CN213407902U CN213407902U CN202021823896.0U CN202021823896U CN213407902U CN 213407902 U CN213407902 U CN 213407902U CN 202021823896 U CN202021823896 U CN 202021823896U CN 213407902 U CN213407902 U CN 213407902U
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Abstract
The utility model provides a nucleic acid extraction appearance is with filtering post, its characterized in that includes barrel, first spout, second spout, third spout, first filtering component, second subassembly, third filtering component, the utility model discloses increase first spout, first slider, second spout, second slider, third spout, third slider, sealing strip, locking screw, realized the slidable mounting and dismantlement of first filter piece, realized the slidable mounting and the dismantlement of second filter piece, realized the slidable mounting and the dismantlement of third filter piece, reduced the burden of installation dismantlement for the staff, make first filter piece, second filter piece, third filter piece in this application convenient to dismantle the installation more, can not cause the damage to it when dismantling the installation, can not influence its filter effect, reduced economic cost, prolonged life; the locking screw rod realizes locking and fixing of the first sliding groove and the first sliding block, the second sliding groove and the second sliding block, and the third sliding groove and the third sliding block.
Description
Technical Field
The utility model relates to a nucleic acid detects technical field, especially relates to nucleic acid extraction appearance is with filtering post.
Background
Nucleic acid includes plasmid DNA, when extracting plasmid DNA, solid phase impurities except plasmid need to be filtered and removed, and the solid phase impurities include denatured genome DNA and protein fusion, etc.; the most common method for filtering impurities during extraction of plasmid DNA is a filtering method, the filtering method usually adopts a syringe type nucleic acid filtering column, a microporous filtering material is arranged in the syringe type nucleic acid filtering column, filtering holes are formed in the microporous filtering material, during filtering, the surface of a solution to be filtered in the syringe is pressurized by pushing a push cylinder in the syringe type nucleic acid filtering column, so that the solution flows through the filtering holes of the microporous filtering material, solid-phase impurities in the solution to be filtered are filtered in the nucleic acid filtering column, and the subsequent operation is carried out after the solution containing the plasmid DNA passes through the filtering material; however, the aperture of the filtering hole formed in the microporous filtering material is small, generally about 10-15 μm, and in the filtering process, the filtering hole is easily blocked by large solid-phase impurities, which can increase the resistance of the plasmid DNA solution to be extracted passing through the filtering hole, thereby affecting the filtering speed, and meanwhile, after the filtering hole is blocked, part of the solution is retained on the microporous filtering material, so that the plasmid DNA solution to be extracted cannot be completely filtered, therefore, technical personnel in the related field further improve the filtering column, so that the solid-phase impurities can be thoroughly filtered, and the plasmid-following liquid phase can smoothly and quickly pass through a plurality of filtering pieces, thereby improving the filtering efficiency;
in the prior art, a nucleic acid solution to be extracted sequentially passes through a plurality of filter elements, so that solid-phase impurities are completely intercepted by the filter elements in sequence according to the size of the volume, and the nucleic acid can smoothly and rapidly sequentially pass through the filter elements along with a liquid phase, thereby improving the filtering efficiency during nucleic acid extraction;
in the prior art, the plurality of filtering elements are arranged on the side wall of the filtering column, after nucleic acid is filtered for a plurality of times, a large amount of intercepted particle impurities are attached to the surfaces of the plurality of filtering elements, and each filtering element needs to be taken out to be cleaned or replaced in order not to influence the later filtering effect, however, a filtering component in the prior art is arranged in a clamping groove of the side wall of the filtering column, and a worker needs to stretch into the filtering column by two hands to draw out the filtering element from the clamping groove, so that the difficulty of disassembling the filtering element is increased, the filtering element is not convenient to disassemble, the phenomenon that the filtering element is damaged in the process of drawing out the filtering element from the clamping groove is caused, the filtering effect of the filtering element is reduced, the economic cost is further increased, the service life of the filtering element is shortened to a certain extent, and therefore, we propose a filter column for a nucleic acid extractor.
Disclosure of Invention
According to the above technical problem, the utility model provides a nucleic acid extraction appearance is with filtering post, its characterized in that includes barrel, first spout, second spout, third spout, first filtering component, second subassembly, third filtering component, the inside wall symmetry of barrel has seted up first spout, the inside wall symmetry of barrel has seted up second spout, the inside wall symmetry of barrel has seted up third spout, the second spout, third spout have symmetrically been seted up between two said first spouts, the third spout has been seted up between one said first spout and one said second spout, the third spout has been seted up between another said first spout and another second spout; a first filtering assembly is arranged in the first sliding groove, a second filtering assembly is arranged in the second sliding groove, and a third filtering assembly is arranged in the third sliding groove;
the first filtering component consists of first sliding blocks, sealing strips, locking screws, an installation frame and first filtering pieces, the first sliding blocks are respectively installed in the two first sliding grooves, the sealing strips are respectively arranged on the side walls of the two first sliding blocks, the sealing strip is made of elastic materials, the outer side walls of the two first sliding blocks are respectively provided with a groove, the two grooves are respectively connected with the two ends of the mounting frame, a first filter element is arranged in the mounting frame, a mounting hole is arranged at the upper end of the inner part of the first sliding block, the first chute is internally provided with mounting holes, the two mounting holes are arranged on the same straight line, the inner side wall of the mounting hole is provided with threads, a locking screw is mounted in the mounting hole, the outer side wall of the locking screw is provided with threads matched with the mounting hole, and the first sliding block is fixed with the first sliding groove through the locking screw in the mounting hole;
the second filtering component consists of a second sliding block, a sealing strip, a locking screw, an installation frame and a second filtering piece, the second sliding blocks are respectively installed in the two second sliding grooves, the sealing strip is respectively arranged on the side wall of the two second sliding blocks, the outer side wall of the two second sliding blocks is respectively provided with a groove, the two grooves are respectively connected with the two ends of the installation frame, a second filter element is arranged in the mounting frame, the upper end of the inner part of the second sliding block is provided with a mounting hole, the second chute is internally provided with mounting holes, the two mounting holes are arranged on the same straight line, the inner side wall of the mounting hole is provided with threads, a locking screw is mounted in the mounting hole, the outer side wall of the locking screw is provided with threads matched with the mounting hole, and the second sliding block is fixed with the second sliding groove through the locking screw in the mounting hole;
the third filtering component consists of third sliding blocks, sealing strips, locking screws, an installation frame and third filtering pieces, the third sliding blocks are respectively installed in the two third sliding grooves, the sealing strips are respectively arranged on the side walls of the two third sliding blocks, grooves are respectively formed in the outer side walls of the two third sliding blocks, the two grooves are respectively connected with the two ends of the installation frame, a third filter element is arranged in the mounting frame, the upper end of the inner part of the third sliding block is provided with a mounting hole, the third chute is internally provided with mounting holes, the two mounting holes are arranged on the same straight line, the inner side wall of the mounting hole is provided with threads, a locking screw is mounted in the mounting hole, the outer side wall of the locking screw is provided with threads matched with the mounting hole, and the third sliding block is fixed with the third sliding groove through the locking screw in the mounting hole;
the length of the first sliding block is greater than that of the second sliding block;
the length of the second sliding block is greater than that of the third sliding block;
the length of the first sliding chute is greater than that of the second sliding chute;
the length of the second sliding chute is greater than that of the third sliding chute.
The utility model has the advantages that: the utility model discloses increase first spout, first slider, the second spout, the second slider, the third spout, the third slider, the sealing strip, locking screw, use through the cooperation of first spout and first slider, the slidable mounting and the dismantlement of first filter piece have been realized, the cooperation of second spout and second slider is used, the slidable mounting and the dismantlement of second filter piece have been realized, the cooperation of third spout and third slider is used, the slidable mounting and the dismantlement of third filter piece have been realized, the burden of installation dismantlement has been reduced for the staff, make the first filter piece, the second filter piece, the third filter piece in this application be convenient for more dismantle the installation, and, can not cause the damage to it when dismantling the installation, can not influence its filter effect, economic cost is reduced, and service life is prolonged; locking screw has realized fixed the locking of first spout and first slider, second spout and second slider, third spout and third slider, the sealing strip is realized between first spout and the first slider, between second spout and the second slider, effective sealing between third spout and the third slider, prevent that nucleic acid liquid from getting into first spout, the second spout, the inside of third spout, sealed effect has been played, the sealing strip is the elasticity material simultaneously, can effectively improve first slider, the second slider, the stability of third slider installation.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
fig. 2 is a top view of the side wall of the cylinder body of the present invention with a first chute, a second chute and a third chute;
fig. 3 is a top view of the first filter assembly, the second filter assembly and the third filter assembly installed inside the cylinder of the present invention;
FIG. 4 is a schematic structural view of a first filter assembly of the present invention;
FIG. 5 is a schematic structural view of a second filter assembly according to the present invention;
FIG. 6 is a schematic structural view of a third filter assembly according to the present invention;
fig. 7 is a schematic view of the sealing strip arranged at the bottom of the first sliding block of the present invention;
fig. 8 is a schematic view of the first sliding groove and the first sliding block being locked by the locking screw rod.
As shown in the figure, 1-cylinder, 10-first chute, 11-second chute, 12-third chute, 2-first filter assembly, 20-first slide block, 21-sealing strip, 22-locking screw rod, 23-mounting frame, 24-first filter element, 3-second filter assembly, 30-second slide block, 31-second filter element, 4-third filter assembly, 40-third slide block and 41-third filter element.
Detailed Description
Example 1
The utility model provides a nucleic acid extraction appearance is with filtering post, its characterized in that includes barrel 1, first spout 10, second spout 11, third spout 12, first filtering component 2, second subassembly, third filtering component 4, first spout 10 has been seted up to the inside wall symmetry of barrel 1, second spout 11 has been seted up to the inside wall symmetry of barrel 1, third spout 12 has been seted up to the inside wall symmetry of barrel 1, second spout 11, third spout 12 have been seted up to the symmetry between two first spouts 10, third spout 12 has been seted up between one first spout 10 and one second spout 11, third spout 12 has been seted up between another first spout 10 and another second spout 11; a first filtering component 2 is arranged in the first sliding chute 10, a second filtering component 3 is arranged in the second sliding chute 11, and a third filtering component 4 is arranged in the third sliding chute 12;
the first filtering component 2 is composed of first sliding blocks 20, sealing strips 21, locking screws 22, an installation frame 23 and first filtering pieces 24, the first sliding blocks 20 are respectively installed in the two first sliding grooves 10, the sealing strips 21 are respectively arranged on the side walls of the two first sliding blocks 20, the sealing strips 21 are made of elastic materials, grooves are respectively formed in the outer side walls of the two first sliding blocks 20, the two grooves are respectively connected with the two ends of the installation frame 23, the first filtering pieces 24 are installed inside the installation frame 23, installation holes are formed in the upper ends of the insides of the first sliding blocks 20, installation holes are formed inside the first sliding grooves 10, the two installation holes are formed in the same straight line, the inner side wall of the mounting hole is provided with threads, a locking screw 22 is installed in the mounting hole, the outer side wall of the locking screw 22 is provided with threads matched with the mounting hole, and the first sliding block 20 is fixed with the first sliding chute 10 through the locking screw 22 in the mounting hole;
the second filtering component 3 is composed of a second sliding block 30, a sealing strip 21, a locking screw 22, an installation frame 23 and a second filtering piece 31, the second sliding blocks 30 are respectively installed in the two second sliding grooves 11, the sealing strip 21 is respectively arranged on the side walls of the two second sliding blocks 30, grooves are respectively arranged on the outer side walls of the two second sliding blocks 30, the two grooves are respectively connected with the two ends of the installation frame 23, the second filtering piece 31 is installed inside the installation frame 23, an installation hole is arranged at the upper end inside the second sliding block 30, an installation hole is arranged inside the second sliding groove 11, the two installation holes are arranged on the same straight line, the inner side wall of the mounting hole is provided with threads, a locking screw 22 is installed in the mounting hole, the outer side wall of the locking screw 22 is provided with threads matched with the mounting hole, and the second sliding block 30 is fixed with the second sliding chute 11 through the locking screw 22 in the mounting hole;
the third filtering component 4 is composed of a third sliding block 40, a sealing strip 21, a locking screw 22, an installation frame 23 and a third filtering component 41, the third sliding blocks 40 are respectively installed in the two third sliding grooves 12, the sealing strip 21 is respectively arranged on the side walls of the two third sliding blocks 40, grooves are respectively arranged on the outer side walls of the two third sliding blocks 40, the two grooves are respectively connected with the two ends of the installation frame 23, the third filtering component 41 is installed inside the installation frame 23, an installation hole is arranged at the upper end inside the third sliding block 40, an installation hole is arranged inside the third sliding groove 12, the two installation holes are arranged on the same straight line, the inner side wall of the mounting hole is provided with threads, a locking screw 22 is installed in the mounting hole, the outer side wall of the locking screw 22 is provided with threads matched with the mounting hole, and the third sliding block 40 is fixed with the third sliding chute 12 through the locking screw 22 in the mounting hole;
the length of the first slider 20 is greater than the length of the second slider 30;
the length of the second slider 30 is greater than the length of the third slider 40;
the length of the first chute 10 is greater than that of the second chute 11;
the length of the second chute 11 is greater than the length of the third chute 12.
Example 2
The utility model discloses after using a period, first filter 24, second filter 31, the surface of third filter 41 can adhere to a large amount of impurity, in order not to influence the filtration efficiency of this application, need to dismantle first filter 24, second filter 31, third filter 41 and clear up or change, at first, twist off locking screw 22 on the third slider 40 from the mounting hole, make third slider 40 and third spout 12 be in locking state, the staff stimulates third slider 40 and upwards moves in third spout 12, make third slider 40 pull out from third spout 12, make the third filter 41 in the installation frame 23 taken out, the dismantlement of third filter 41 has been accomplished promptly; then, the locking screw 22 on the second slider 30 is screwed off from the mounting hole, so that the second slider 30 and the second chute 11 are in a locked state, and the worker pulls the second slider 30 in the second chute 11 and moves upward, so that the second slider 30 is pulled out from the second chute 11, so that the second filter member 31 in the mounting frame 23 is extracted, that is, the detachment of the second filter member 31 is completed; finally, the locking screw 22 on the first slider 20 is screwed off from the mounting hole, so that the first slider 20 and the first chute 10 are in a locked state, and the worker pulls the first slider 20 in the first chute 10 and moves upwards, so that the first slider 20 is pulled out from the first chute 10, the first filter member 24 in the mounting frame 23 is pulled out, and the first filter member 24 is completely detached; through the matching use of the first chute 10 and the first slider 20, the sliding installation and the disassembly of the first filtering piece 24 are realized, the matching use of the second chute 11 and the second slider 30 is realized, the sliding installation and the disassembly of the second filtering piece 31 are realized, the matching use of the third chute 12 and the third slider 40 is realized, the sliding installation and the disassembly of the third filtering piece 41 are realized, the installation and disassembly burden is reduced for workers, so that the first filtering piece 24, the second filtering piece 31 and the third filtering piece 41 in the application are more convenient to disassemble and assemble, in addition, the first filtering piece 24, the second filtering piece 31 and the third filtering piece 41 cannot be damaged during the disassembly and the assembly, the filtering effect cannot be influenced, the economic cost is reduced, and the service life is prolonged;
The basic principles and the main features of the invention and the advantages of the invention have been shown and described above. The utility model discloses each part that mentions is the common technique in prior art, and the technical personnel of this trade should understand, the utility model discloses do not receive the restriction of above-mentioned embodiment, the description only is the explanation in above-mentioned embodiment and the description the principle of the utility model, under the prerequisite that does not deviate from the spirit and the scope of the utility model, the utility model discloses still can have various changes and improvement, these changes and improvement all fall into the protection of claim the utility model is within the scope. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (6)
1. The filter column for the nucleic acid extraction instrument is characterized by comprising a cylinder body, first sliding grooves, second sliding grooves, third sliding grooves, first filter assemblies, second assemblies and third filter assemblies, wherein the inner side wall of the cylinder body is symmetrically provided with the first sliding grooves, the inner side wall of the cylinder body is symmetrically provided with the second sliding grooves, the inner side wall of the cylinder body is symmetrically provided with the third sliding grooves, the second sliding grooves and the third sliding grooves are symmetrically arranged between the two first sliding grooves, the third sliding groove is arranged between one first sliding groove and one second sliding groove, and the third sliding groove is arranged between the other first sliding groove and the other second sliding groove; a first filtering assembly is arranged in the first sliding groove, a second filtering assembly is arranged in the second sliding groove, and a third filtering assembly is arranged in the third sliding groove;
the first filtering component consists of first sliding blocks, sealing strips, locking screws, an installation frame and first filtering pieces, the first sliding blocks are respectively installed in the two first sliding grooves, the sealing strips are respectively arranged on the side walls of the two first sliding blocks, grooves are respectively formed in the outer side walls of the two first sliding blocks, the two grooves are respectively connected with the two ends of the installation frame, a first filter element is arranged in the mounting frame, a mounting hole is arranged at the upper end of the inner part of the first sliding block, the first chute is internally provided with mounting holes, the two mounting holes are arranged on the same straight line, the inner side wall of the mounting hole is provided with threads, a locking screw is mounted in the mounting hole, the outer side wall of the locking screw is provided with threads matched with the mounting hole, and the first sliding block is fixed with the first sliding groove through the locking screw in the mounting hole;
the second filtering component consists of a second sliding block, a sealing strip, a locking screw, an installation frame and a second filtering piece, the second sliding blocks are respectively installed in the two second sliding grooves, the sealing strip is respectively arranged on the side wall of the two second sliding blocks, the outer side wall of the two second sliding blocks is respectively provided with a groove, the two grooves are respectively connected with the two ends of the installation frame, a second filter element is arranged in the mounting frame, the upper end of the inner part of the second sliding block is provided with a mounting hole, the second chute is internally provided with mounting holes, the two mounting holes are arranged on the same straight line, the inner side wall of the mounting hole is provided with threads, a locking screw is mounted in the mounting hole, the outer side wall of the locking screw is provided with threads matched with the mounting hole, and the second sliding block is fixed with the second sliding groove through the locking screw in the mounting hole;
the third filtering component consists of third sliding blocks, sealing strips, locking screws, an installation frame and third filtering pieces, the third sliding blocks are respectively installed in the two third sliding grooves, the sealing strips are respectively arranged on the side walls of the two third sliding blocks, grooves are respectively formed in the outer side walls of the two third sliding blocks, the two grooves are respectively connected with the two ends of the installation frame, a third filter element is arranged in the mounting frame, the upper end of the inner part of the third sliding block is provided with a mounting hole, the third chute is internally provided with mounting holes, the two mounting holes are arranged on the same straight line, the inner side wall of the mounting hole is provided with threads, a locking screw is mounted in the mounting hole, the outer side wall of the locking screw is provided with threads matched with the mounting hole, and the third sliding block is fixed with the third sliding groove through the locking screw in the mounting hole.
2. The filter column for nucleic acid isolation apparatus according to claim 1, wherein the sealing strip is made of an elastic material.
3. The filter cartridge for a nucleic acid isolation apparatus according to claim 1, wherein the length of the first slider is longer than the length of the second slider.
4. The filter cartridge for a nucleic acid isolation apparatus according to claim 1, wherein the length of the second block is longer than the length of the third block.
5. The filter cartridge for a nucleic acid isolation apparatus according to claim 1, wherein the length of the first slide groove is longer than the length of the second slide groove.
6. The filter cartridge for a nucleic acid isolation apparatus according to claim 1, wherein the length of the second slide groove is longer than the length of the third slide groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021823896.0U CN213407902U (en) | 2020-08-27 | 2020-08-27 | Filtering column for nucleic acid extractor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021823896.0U CN213407902U (en) | 2020-08-27 | 2020-08-27 | Filtering column for nucleic acid extractor |
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| Publication Number | Publication Date |
|---|---|
| CN213407902U true CN213407902U (en) | 2021-06-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021823896.0U Active CN213407902U (en) | 2020-08-27 | 2020-08-27 | Filtering column for nucleic acid extractor |
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| CN (1) | CN213407902U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114028854A (en) * | 2021-12-11 | 2022-02-11 | 郯城泽华基础工程有限公司 | Seawater desalination treatment facility convenient to clearance sweeps |
-
2020
- 2020-08-27 CN CN202021823896.0U patent/CN213407902U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114028854A (en) * | 2021-12-11 | 2022-02-11 | 郯城泽华基础工程有限公司 | Seawater desalination treatment facility convenient to clearance sweeps |
| CN114028854B (en) * | 2021-12-11 | 2022-11-15 | 郯城泽华基础工程有限公司 | Seawater desalination treatment facility convenient to clearance sweeps |
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