CN213357550U - Reaction bag sampling device - Google Patents
Reaction bag sampling device Download PDFInfo
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- CN213357550U CN213357550U CN202120782609.4U CN202120782609U CN213357550U CN 213357550 U CN213357550 U CN 213357550U CN 202120782609 U CN202120782609 U CN 202120782609U CN 213357550 U CN213357550 U CN 213357550U
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Abstract
The utility model discloses a reaction bag sampling device, include: an insertion part and a sampling part; the front end of the insertion part is provided with a first hose which is arranged inside the reaction bag; the rear end of the first hose is fixed through the adapter; the sampling part comprises a three-way joint, one end of the three-way joint is connected with the adapter through a pipeline, and the other two ends of the three-way joint are respectively connected with the perfusion drainage pipeline and the sampling pipeline; the perfusion and drainage pipeline is provided with a pipe clamp, and the tail end of the perfusion and drainage pipeline is provided with an air filter; the sampling pipeline is connected with a one-way valve in series and is provided with a pipe clamp, and the tail end of the sampling pipeline is connected with a second sampler in a rotating mode; the utility model has the advantages that the insertion part directly extends into the reaction bag, so that the wave reactor can be used for normal sampling in the shaking process, the sampling convenience is increased, and the sampling is not required to be stopped, thereby being beneficial to maintaining the stability of cell culture; the effect of continuously and uninterruptedly discharging culture solution can be achieved during perfusion culture, and experimental errors are reduced; and the design of the one-way valve is combined, so that the pollution risk during sampling is reduced.
Description
Technical Field
The utility model relates to a microbiological technology field especially relates to a reaction bag sampling device.
Background
Present bio-pharmaceuticals field, it is common to carry out seed preparation or perfusion culture with WAVE (WAVE) bioreactor, and it utilizes the mode of swaing to carry out cell culture, compares that the jar body reactor shearing force that uses the stirring rake comes for a short time, and uses disposable reaction bag, and the equipment is convenient. However, the manufacturer provides a standard reaction bag with a sampling device located above the bag, and the device needs to be stopped and swung during sampling, so that the culture solution can be sampled while being inclined. In addition to the inconvenience of sampling, the wave bioreactor may cause dissolved oxygen fluctuation for a culture solution with high cell density in view of the fact that the wave bioreactor performs gas exchange by means of swing, and the swing is interrupted. And the sampling device of the standard reaction bag has no one-way valve, which also increases the risk of contamination during sampling.
In addition, in perfusion culture, it is necessary to continuously pump out the culture medium in a swing state to maintain the cell density in the bag, but the conventional apparatus of the standard reaction bag cannot achieve the purpose, and the phenomenon of liquid and gas interleaving is caused, so that the experimental error is large.
Chinese patent CN205473764U discloses a biological reaction bag sampling pipe; the waste liquid bottle is provided with a first silicone tube and a second silicone tube in a communicated manner at the bottle opening, and the first silicone tube is respectively connected with a third silicone tube and a fourth silicone tube through a three-way joint; the third silicone tube is connected with the C-Flex tube through a two-way joint, and the C-Flex tube is provided with a first gas sterilizing filter; and the sampling bottle group is respectively connected with a fourth silicone tube and a fifth silicone tube, and a second gas sterilization filter is arranged on the fifth silicone tube. The above technical solution solves the problem of contamination prevention in the subsequent sampling process, however, the technical solution does not solve the problem of continuous sampling, and therefore further improvement is required.
SUMMERY OF THE UTILITY MODEL
In view of this, the utility model provides a reaction bag sampling device can solve above-mentioned problem.
For this purpose, the utility model is implemented by the following technical scheme.
A reaction bag sampling device mainly comprises: an insertion part and a sampling part; the insertion part is connected in series with the sampling part;
the front end of the insertion part is provided with a first hose which is arranged inside the reaction bag; the front end of the first hose is lapped on the bottom surface of the reaction bag, the rear end of the first hose is fixed on a sampling port of the reaction bag through an adapter, and the adapter is positioned outside the reaction bag;
the adapter is connected with the sampling part through a pipeline; the sampling part comprises a three-way joint, one end of the three-way joint is connected with the adapter through a pipeline, and the other two ends of the three-way joint are respectively connected with a perfusion drainage pipeline and a sampling pipeline;
the tail end of the perfusion liquid discharge pipeline is provided with an air filter, and the perfusion liquid discharge pipeline is provided with a pipe clamp;
the sampling pipeline is connected with a one-way valve in series, and the tail end of the sampling pipeline is connected with a second sampler in a switching mode; and a pipe clamp is arranged on the sampling pipeline.
Further, the adapter is public head adapter, with the female head adapter of the sample connection of reaction bag is connected, first hose passes the sample connection.
Furthermore, a second hose, a first female adapter and a first sampler are sequentially connected between the adapter and the tee joint in series; the first sampler is a threaded sampling port; the first female adapter is connected with the first sampler; the first hose outer diameter is smaller than the second hose inner diameter; the first hose passes in proper order the inside of second hose, first female joint adapter, and with the inlet fixed connection of first sampler.
Furthermore, a first male adapter and a third hose are connected between the first sampler and the three-way joint in series; the first male adapter is connected with the first sampler.
Further, the main body of the perfusion and drainage pipeline is a fourth hose, and the tail end of the fourth hose is connected with a thermoplastic pipe through a single-way joint; the tail end of the thermoplastic pipe is provided with the air filter; a pipe clamp is arranged on the fourth hose.
Further, the sampling pipeline main body is a fifth hose, and the tail end of the fifth hose is connected with a sixth hose through the one-way valve; the second sampler is the screw thread sample connection, the sixth hose is terminal to be connected through the female first adapter of second the second sampler.
Furthermore, the valve body of the one-way valve is of a buckling structure and comprises an upper valve body and a lower valve body; the lower valve body is a liquid inlet end of the one-way valve, and the upper valve body is a liquid outlet end of the one-way valve;
a sealing ring is arranged on the contact end surface of the upper valve body and the lower valve body, and a cavity is arranged between the upper valve body and the lower valve body; the liquid outlet of the lower valve body protrudes towards the cavity to form a conical round platform structure; the liquid outlet is sleeved with an elastic part; the elastic part is of a round platform structure of the drawing shell, and the inner side face of the elastic part is matched with the outer side face of the liquid outlet.
Furthermore, the first hose and the pipeline are silica gel hoses.
The reaction bag in the utility model is a special disposable reaction bag on the WAVE bioreactor.
The utility model has the advantages of as follows:
1. the first hose is directly stretched into the reaction bag through the insertion part, the front end of the first hose is lapped on the bottom surface of the reaction bag, and liquid does not pass through the front end of the first hose, so that the wave reactor can be normally sampled in the shaking process, the sampling convenience is improved, and the cell culture stability can be maintained without stopping sampling; the effect of continuously and uninterruptedly discharging culture solution can be achieved during perfusion culture, and experimental errors are reduced;
2. the utility model discloses a newly-increased check valve in the sample pipeline prevents the liquid reflux, the risk of pollution when reducing the sample.
Drawings
In order to illustrate the embodiments of the present invention more clearly, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only one or several embodiments of the present invention, and that other drawings can be obtained by those skilled in the art without inventive efforts.
FIG. 1 is a schematic structural view of the present invention;
fig. 2 is an exploded view of an insertion portion in embodiment 1 of the present invention;
FIG. 3 is an exploded view of a sampling portion in example 1 of the present invention;
fig. 4 is a cross-sectional view of the check valve in embodiment 2 of the present invention;
fig. 5 is a top view of the upper valve body in embodiment 2 of the present invention.
In the figure:
1-an insertion portion; 2-a sampling part; 3-reaction bag; 101-a first hose; 102-an adapter; 103-a second hose; 104-a first female adapter; 105-a first sampler; 201-a first male adaptor; 202-a third hose; 203-three-way connection; 204-pipe clamp; 205-a fifth hose; 206-one-way valve; 207-sixth hose; 208-a second female adapter; 209-a second sampler; 210-a fourth hose; 211-single pass junction; 212-thermoplastic tube; 213-an air filter; 2061-upper valve body; 2062-a boss; 2063-an elastic member; 2064-sealing ring; 2065-lower valve body; 2066-liquid outlet.
Detailed Description
The conception, specific structure and technical effects of the present invention will be described clearly and completely with reference to the accompanying drawings and embodiments, so as to fully understand the objects, aspects and effects of the present invention. It should be noted that, in the case of no conflict, the features in the embodiments of the present application may be combined with each other.
It should also be noted that, in the description of the present invention, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and the like are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
The present invention will be further explained with reference to the accompanying drawings.
At first right the utility model discloses it is necessary to introduce to use the basis, and WAVE formula that WAVE WAVE bioreactor adopted non-intervention shakes the mixture, has avoided stirring paddle end and tympanic bulla to the injury of cell, provides the cell culture microenvironment of the high dissolved oxygen of gentle low shear force, is favorable to improving cell state, improves cell density and output. The reaction bag 3 is a sterile cell culture bag, so that cleaning and sterilization are not needed, the pollution risk is reduced, and the success rate of cell culture is improved. The WAVE WAVE bioreactor has the advantages of flexible culture volume range, simple operation, precise and reliable control and easy process amplification. The general design is adopted, the swing platform is connected through hinges and the like in the platform technology, the swing platform is switched by an external driver, the swing platform periodically swings, and the reaction bags 3 are placed on the platform.
Example 1
As shown in fig. 1, a reaction bag sampling device mainly comprises: an insertion part 1 and a sampling part 2; the insertion part 1 and the sampling part 2 are connected in series;
as shown in fig. 2, the first hose 101, specifically a silicone tube with a specification of 1/25 inches × 17/200 inches, is provided at the tip of the insertion portion 1, and is inserted through the sampling port of the reaction bag 3 and attached to the inside of the reaction bag 3. The front end of the first hose is lapped on the bottom surface of the reaction bag 3, and the adapter 102 is positioned outside the reaction bag 3; preferably, the adapter 102 is a male adapter, which is connected to a female adapter of the sampling port of the reaction bag 3. Furthermore, the insertion portion 1 further includes a second hose 103, a first female adapter 104, and a first sampler 105, which are sequentially connected in series between the adapter 102 and the three-way adapter 203. The second hose 103 is a silicone tube with specification of 1/8 inches x1/4 inches, and meets the size requirements of male and female heads. The first sampler 105 is a threaded sampling port so that sampling can be performed in the existing manner even if subsequent lines are not connected. The size of the liquid inlet of the first sampler 105 is smaller than the size of a connecting pipeline required by the male-female joint, and in order to realize switching, the first hose 101 sequentially passes through the adapter 102, the second hose 103, the first female adapter 104 and is fixedly connected with the liquid inlet of the first sampler 105, and the first female adapter 104 is externally connected with the first sampler 105, so that the fixation of each component of the insertion part 1 is completed, which is equivalent to the fact that the first hose 101 is indirectly fixed on the sampling port of the reaction bag 3 through the adapter 102. The design can also realize the function of conveniently replacing the rear end pipeline; meanwhile, the existing market-sold specification parts are fully utilized, and a male adapter and a female adapter do not need to be designed independently.
The adapter 102 is connected with the sampling part 2 through a pipeline; the sampling part 2 comprises a three-way joint 203, one end of the three-way joint 203 is connected with the adapter 102 through a pipeline, and the other two ends are respectively connected with a perfusion drainage pipeline and a sampling pipeline; preferably, a first male adapter 201 and a third hose 202 are further connected in series between the first sampler 105 and the three-way joint 203; the first male adapter 201 is connected to the first sampler 105. Wherein, the third hose 202 can be selected from silicone tube with specification of 1/8 inches x1/4 inches.
As shown in fig. 3, the main body of the perfusion drainage pipeline is a fourth flexible pipe 210, and the end of the fourth flexible pipe 210 is connected with a thermoplastic pipe 212 through a single-way joint 211; the end of the thermoplastic pipe 212 is provided with an air filter 213; a clamp 204 is provided on the fourth hose 210. Wherein, the fourth hose 210 is a silicone tube with specification of 1/8 inches x1/4 inches, and the thermoplastic tube 212 is also 1/8 inches x1/4 inches.
As shown in FIG. 2, the sampling pipe is a fifth hose 205, the end of the fifth hose 205 is connected to a sixth hose 207 through a check valve 206, and the fifth hose 205 is further provided with a pipe clamp 204. The second sampler 209 is a threaded sampling port, and the end of the sixth hose 207 is connected to the second sampler 209 through a second female adapter 208. Wherein, silicone tubes with specification of 1/8 inches x1/4 inches are selected for the fifth hose 205 and the sixth hose 207.
Example 2
On the basis of embodiment 1, in this embodiment, the valve body of the check valve 206 is specifically a snap-fit structure, as shown in fig. 4, and includes an upper valve body 2061 and a lower valve body 2065. The lower valve body 2065 is a liquid inlet end of the one-way valve, and the upper valve body 2061 is a liquid outlet end of the one-way valve; the direction of fluid flow is shown by the arrows in figure 4.
Wherein, the contact end surface of the upper valve body 2061 and the lower valve body 2065 is provided with a sealing ring 2064. After the upper valve body 2061 and the lower valve body 2065 are fastened, a cylindrical cavity is formed therebetween for mounting the check member. Wherein, the liquid outlet 2066 of the lower valve body 2065 is protruded towards the cavity to form a cone-shaped round table structure; an elastic member 2063 is sleeved on the liquid outlet 2066; the elastic member 2063 is a round platform structure of the drawing shell, and the inner side surface is matched with the outer side surface of the liquid outlet 2066. The elastic member 2063 functions as a check member to prevent the reverse flow in order to allow the upstream fluid to normally flow out; referring to fig. 5, a plurality of bosses 2062 protruding radially inward are designed at the liquid inlet of the upper valve body 2061, and axially through slots are formed between adjacent bosses 2062, so that the inner cavity of the upper valve body 2061 is communicated with the cavity, and the bosses 2062 limit the movement of the elastic member 2063, and may be designed such that the lower surface of the boss 2062 directly contacts the upper surface of the elastic member 2063, or is in clearance fit with the upper surface of the elastic member 2063. In order to increase the sealing performance, a hemispherical structure may be designed on the upper surface of the inner side of the elastic member 2063. The above design is convenient for processing and assembly, and when the pressure of the lower fluid is greater than the pressure of the upper fluid by a certain threshold, the elastic member 2063 is compressed and moves upwards, and the fluid flows out from between the elastic member 2063 and the liquid outlet 2066, enters the cavity, enters the inner cavity of the upper valve body through the notch and finally flows out; when the difference value between the pressure intensity of the upper fluid and the pressure intensity of the lower fluid is smaller than the threshold value, the hemispheroid rebounds to rapidly block the passage, the range of the flow path acted by the elastomer is prolonged, backflow is reduced, the rebound speed of the upper part is higher than that of the lower part by virtue of the conical structure, backflow fluid in the cavity is extruded out from the side direction, the backflow amount can be effectively controlled, and biological pollution is reduced.
The above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the examples, those skilled in the art should understand that the technical solutions of the present invention can be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the scope of the claims of the present invention.
Claims (8)
1. A reaction bag sampling device, comprising: an insertion section (1) and a sampling section (2); the insertion part (1) and the sampling part (2) are connected in series;
the front end of the insertion part (1) is provided with a first hose (101), and the first hose (101) is arranged inside the reaction bag (3); the front end of a first hose is lapped on the bottom surface of the reaction bag (3), the rear end of the first hose is fixed on a sampling port of the reaction bag (3) through an adapter (102), and the adapter (102) is positioned outside the reaction bag (3);
the adapter (102) is connected with the sampling part (2) through a pipeline; the sampling part (2) comprises a three-way joint (203), one end of the three-way joint (203) is connected with the adapter (102) through a pipeline, and the other two ends of the three-way joint are respectively connected with a perfusion drainage pipeline and a sampling pipeline;
an air filter (213) is arranged at the tail end of the perfusion liquid discharge pipeline, and a pipe clamp (204) is arranged on the perfusion liquid discharge pipeline;
the sampling pipeline is connected with a one-way valve (206) in series, and the tail end of the sampling pipeline is connected with a second sampler (209) in a switching mode; and a pipe clamp (204) is arranged on the sampling pipeline.
2. A sampling device according to claim 1, characterized in that the adapter (102) is a male adapter, which is connected to a female adapter of a sampling opening of the reaction bag (3), through which the first hose (101) is passed.
3. The sampling device according to claim 2, characterized in that a second hose (103), a first female adapter (104) and a first sampler (105) are connected in series between the adapter (102) and the tee joint (203); the first sampler (105) is a threaded sampling port; the first female adapter (104) is connected with the first sampler (105); the outer diameter of the first hose (101) is smaller than the inner diameter of the second hose (103); the first hose (101) sequentially penetrates through the second hose (103) and the first female adapter (104), and is fixedly connected with the liquid inlet of the first sampler (105).
4. The sampling device according to claim 3, characterized in that a first male adapter (201) and a third hose (202) are connected in series between the first sampler (105) and the tee joint (203); the first male adapter (201) is connected with the first sampler (105).
5. The sampling device according to claim 1, wherein the perfusion drainage line body is a fourth hose (210), and the end of the fourth hose (210) is connected with a thermoplastic pipe (212) through a single-way joint (211); the end of the thermoplastic pipe (212) is provided with the air filter (213); the fourth hose (210) is provided with a pipe clamp (204).
6. The sampling device according to claim 1, characterized in that the sampling pipe body is a fifth hose (205), the end of the fifth hose (205) is connected with a sixth hose (207) through the one-way valve (206); the second sampler (209) is the screw thread sample connection, sixth hose (207) is terminal to be connected through second female adapter (208) second sampler (209).
7. The sampling device of claim 1, wherein the one-way valve (206) body is a snap-fit structure comprising an upper valve body (2061), a lower valve body (2065); the lower valve body (2065) is a liquid inlet end of the one-way valve, and the upper valve body (2061) is a liquid outlet end of the one-way valve;
a sealing ring (2064) is arranged on the contact end face of the upper valve body (2061) and the lower valve body (2065), and a cavity is arranged between the upper valve body (2061) and the lower valve body (2065); the liquid outlet (2066) of the lower valve body (2065) protrudes towards the cavity to form a conical round table structure; an elastic piece (2063) is sleeved on the liquid outlet (2066); the elastic piece (2063) is of a round table structure of the drawing shell, and the inner side face of the elastic piece is matched with the outer side face of the liquid outlet (2066).
8. A sampling device according to any one of claims 1-7, characterized in that the first hose (101) and the tubing are silicone hoses.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120782609.4U CN213357550U (en) | 2021-04-16 | 2021-04-16 | Reaction bag sampling device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120782609.4U CN213357550U (en) | 2021-04-16 | 2021-04-16 | Reaction bag sampling device |
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| CN213357550U true CN213357550U (en) | 2021-06-04 |
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| CN202120782609.4U Active CN213357550U (en) | 2021-04-16 | 2021-04-16 | Reaction bag sampling device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114874878A (en) * | 2022-07-11 | 2022-08-09 | 浙江金仪盛世生物工程有限公司 | Support frame with two clamping states and biological reaction device |
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2021
- 2021-04-16 CN CN202120782609.4U patent/CN213357550U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114874878A (en) * | 2022-07-11 | 2022-08-09 | 浙江金仪盛世生物工程有限公司 | Support frame with two clamping states and biological reaction device |
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Address after: 215123 unit 101, 201, C37 / F, 218 Xinghu street, Suzhou Industrial Park, Suzhou City, Jiangsu Province Patentee after: Suzhou Yaoming Biotechnology Co.,Ltd. Address before: 215123 unit 101, 201, C37 / F, 218 Xinghu street, Suzhou Industrial Park, Suzhou City, Jiangsu Province Patentee before: Suqiao Biology (Suzhou) Co., Ltd |