CN213327606U - Sample tube for cracking biological sample and molecular in-vitro diagnostic instrument - Google Patents
Sample tube for cracking biological sample and molecular in-vitro diagnostic instrument Download PDFInfo
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- CN213327606U CN213327606U CN202020511549.8U CN202020511549U CN213327606U CN 213327606 U CN213327606 U CN 213327606U CN 202020511549 U CN202020511549 U CN 202020511549U CN 213327606 U CN213327606 U CN 213327606U
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Abstract
The utility model provides a sample cell for schizolysis biological sample, it includes tube cap and shaft, the inner wall of the shaft of sample cell has two at least strip protrudingly. The utility model also provides a molecular external diagnostic instrument including nucleic acid extraction and detection device and automated control system, wherein nucleic acid extraction and detection device's schizolysis module includes aforementioned sample cell that is used for schizolysis biological sample to and the oscillator rather than supporting use.
Description
Technical Field
The utility model relates to a biology and medical device field. Specifically, the utility model provides a sample cell for schizolysis biological sample, the utility model also provides an external diagnostic instrument of molecular body, wherein the schizolysis module of this external diagnostic instrument of molecular body includes aforementioned sample cell that is used for schizolysis biological sample to and the oscillator rather than supporting use.
Background
Cell lysis is the destruction of the cell membrane of a cell and the release of biologically active substances of the cell, such as organelles, enzymes, DNA and RNA, etc. The fluid thus obtained, which contains intracellular components, is called lysate.
In various fields such as modern clinical disease diagnosis, transfusion safety, forensic identification, environmental microorganism detection, food safety detection, molecular biology research, etc., it is required to extract bioactive substances from cells, and thus cell lysis is required to destroy cell membranes.
The natural occurrence of cell lysis includes intracellular viral replication and release, enzymatic lysis of cell membranes, lysis of cells by bursting of cell membranes due to excess water entering the cells as a result of osmotic pressure imbalance, and the like. In addition to natural cell lysis, various methods have been developed for cell lysis in the laboratory. Such as local heating, which causes denaturation of proteins; milling and dispersing cells using rotating blades; shearing cells using high frequency sound waves; repeated cycles of freezing and thawing rupture the cells; and cell cultures frozen in liquid nitrogen were milled manually. The method of solution-based cell lysis comprises: using a hypotonic additive to reduce osmotic pressure to cause cell membrane collapse; use of a hypertonic additive to increase osmotic pressure to burst cell membranes; and the use of detergents.
The mechanical disruption method is a method for applying a wide range of cell disruption methods, and has advantages in that the influence of the added reagent on the subsequent reaction such as nucleic acid amplification reaction such as PCR can be avoided, and the method can be used together with other cell disruption methods.
There is a need in the art for more efficient methods and devices for cell division, particularly for cell division suitable for use in automated nucleic acid extraction instruments.
SUMMERY OF THE UTILITY MODEL
The utility model provides a sample cell for schizolysis biological sample, it includes tube cap and shaft, its characterized in that, the inner wall of the shaft of sample cell has two at least strip protrudingly. The tube cap and the tube body of the sample tube are detachably connected, for example, by a screw connection.
In other embodiments of the present invention, there may be a plurality of axial strip-like protrusions, such as 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 strips, etc. The plurality of axial strip-shaped bulges can be uniformly arranged on the inner wall of the pipe body.
In one aspect of the present invention, the tube cover has a hollow tube cap, an upper portion of which has an outer membrane, and a lower portion of which has an inner membrane.
In yet another aspect of the present invention, the inner membrane of the sample tube has good sealing properties while being easily pierced by the tubular object. When the tubular object stays in the inner membrane, the inner membrane can keep good tightness around the tubular object, and when the tubular object is pulled out, the inner membrane can restore to the original shape to keep the tightness of the sample tube. For example, the inner film is made of rubber or silica gel.
In yet another aspect of the present invention, the inner membrane has a fracture to facilitate insertion of a tubular object through the fracture into the inner membrane. For example, the center of the inner membrane has a cross-hair split.
In yet another aspect of the present invention, the outer membrane is an aluminum-plastic or plastic-sealed membrane. The outer film can prevent liquid from splashing outside in the reagent transportation process. In yet another aspect of the present invention, the outer membrane may have an outer membrane tongue for easy removal by a user.
In yet another aspect of the present invention, the lysis tube contains abrasive particles. The abrasive particles are fine particles having a certain hardness, such as glass beads or ceramic beads. The abrasive particles may have a particle size of about 0.1-1 mm. In operation, the abrasive particles are vortexed with the biological sample to mechanically disrupt the biological sample.
The utility model also provides an automatic molecular in vitro diagnostic apparatus, it includes nucleic acid extraction and detection device to and automatic control system based on computer. Wherein, the nucleic acid extraction and detection device comprises a sample receiving module, a cracking module, a nucleic acid extraction module, a nucleic acid amplification module and a liquid distribution module. The automatic control system can control the liquid distribution module of the molecular in-vitro diagnostic instrument to transfer samples between or in the sample receiving module, the cracking module, the nucleic acid extraction module and the nucleic acid amplification module of the nucleic acid extraction and detection device in a programmed manner, and carry out cracking, extraction, amplification, detection and other procedures according to preset steps under proper conditions, so as to realize the detection of the samples to be detected. The utility model provides a nucleic acid of external diagnostic instrument of molecule draws and detection device's schizolysis module includes aforementioned sample cell that is used for schizolysis biological sample to and the oscillator rather than supporting use.
In yet another aspect of the present invention, the molecular in vitro diagnostic apparatus is used for infection source identification, genetic disease, cancer detection, or genetic variation detection.
Drawings
Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments made with reference to the following drawings:
fig. 1 is a perspective view of an exemplary external structure of a sample tube for lysing a biological sample provided by the present invention.
Fig. 2 is an exploded view of an exemplary sample tube for lysing a biological sample provided by the present invention.
Fig. 3 is a cross-sectional view of an exemplary sample tube for lysing a biological sample provided by the present invention.
Fig. 4 is an exemplary working state diagram of the sample tube and the adapted oscillator for lysing biological samples according to the present invention.
Detailed Description
For a better understanding and explanation of the present invention, the present invention will be described in further detail below with reference to the accompanying drawings.
Example 1
The utility model provides a sample cell for schizolysis biological sample. The utility model provides a sample cell is applicable to the cell membrane in the mode schizolysis biological sample that adopts the physics to grind to release the cell inner material to in the solution. The physical grinding refers to that fine particles with certain hardness, namely grinding particles, such as glass beads or ceramic beads, are oscillated with a sample, and the sample cells are impacted by the rapid motion of the grinding particles, so that cell membranes are broken, substances in the cells are released, and the purpose of mechanically breaking and cracking the sample cells is achieved. Physical milling has a higher lysis efficiency than other cell disruption methods, can lyse cells uniformly and comprehensively, and has less impact on subsequent biochemical or physiological reactions because mechanical lysis does not require the addition of unwanted chemical or biological agents.
The present invention provides a sample tube for lysing a biological sample, which is used for analyzing various biological samples containing a substance to be analyzed (e.g., nucleic acid), particularly containing cells or subcellular components (e.g., vesicles or exosomes). The sample tube for lysing biological samples provided by the present invention can be used for genetic testing including but not limited to genetic testing for human genes and clinical testing for various infectious diseases. The biological sample applied to the methods described herein can be from any source. The biological sample may be a biological material that is separated from its natural environment and comprises polynucleotides, such as a tissue sample, a biological fluid sample, or a cell sample comprising polynucleotides. Biological fluids include, as non-limiting examples, blood, plasma, sputum, urine, cerebrospinal fluid, lavage fluid samples. The biological sample may be from a plant, animal, bacterial or viral source. Biological samples can be obtained from different sources, including but not limited to samples from different individuals, different developmental stages of the same or different individuals, different diseased individuals (e.g., individuals with cancer or suspected of having a genetic disorder), normal individuals, different disease stages of the same or different individuals, individuals treated for different diseases, individuals under different environmental factors, or individuals with a predisposition to a disease, or individuals exposed to an infectious disease agent (e.g., HIV).
Fig. 1 is a perspective view of an exemplary external structure of a sample tube for lysing a biological sample according to the present invention. As shown in fig. 1, the sample tube has a tube cover 1 and a tube body 2. The cap 1 and the shaft 2 are detachably connected, for example by screwing. In one aspect of the invention, the tube cover has internal threads, the tube body has external threads, and the tube cover can be sleeved on the tube body. The utility model provides an inner wall of body of tube 2 of sample cell has the axial strip of two at least symmetries protrudingly. In the exemplary sample tube shown in fig. 1, the stripe-shaped protrusions are made by a film-pressing process of the tube body, thereby forming concave stripes 22 on the outside of the tube body.
Fig. 2 is an exploded view of an exemplary sample tube for lysing a biological sample provided by the present invention. As shown in fig. 2, the tube cover 1 is a double-film cover structure, and has a tube cap 11, the tube cap 11 is a cylinder with an upper opening and a lower opening, the upper part of the tube cap 11 has a removable outer film 12, the lower part has an inner film 13, and the inner film is removably or irremovably connected with the lower part of the tube cap. The cap 11 may have an internal thread for detachable connection with a pipe body having an external thread. In one embodiment of the present invention, the diameter of the top opening of the cap 11 is smaller than the diameter of the cap 11, so as to form a brim 111, which partially covers the top opening, and further avoid the liquid in the tube from splashing out.
The outer membrane 12 of the tube cover can be an aluminum plastic or plastic packaging membrane, so that liquid is prevented from splashing outside in the reagent transportation process. The outer film 12 may have an outer film tongue 121 to facilitate its removal by a user.
The inner membrane 13 of the cap has a good tightness and at the same time is easily pierced by a tubular object, such as a plastic pipette tip or pipette. When the tubular object stays in the inner membrane, the inner membrane can keep good tightness around the tubular object, and when the tubular object is pulled out, the inner membrane can restore to the original shape to keep the tightness of the sample tube. The material of the inner film of the tube cover can be rubber or silica gel. In the embodiment shown in fig. 2, the inner membrane has a cruciform fracture 131 to facilitate insertion and extraction of the tubular object through the fracture in the inner membrane.
Need give the utility model provides a under the operating condition that is used for the sample cell of schizolysis biological sample to add or take out biological sample, can remove the adventitia, then tubulose objects such as pipette head or pipette can pass the intima and carry out the application of sample, when taking out tubulose objects, the intima can scrape the remaining of tub outer wall, avoids the outer wall to hang the liquid and drips and cause the pollution. In addition, the inner membrane can close the system when the sample tube is poured or inverted, so that the contained liquid does not flow outwards, and the pollution is further avoided.
As shown in fig. 2, the tube body of the sample tube for lysing a biological sample provided by the present invention has a cylindrical hollow tube body, which has an external thread 21 at the upper part thereof for detachable connection with a tube cap having an internal thread. The utility model provides a space for holding about 0.2-10ml, for example about 0.5-5 ml's liquid can be provided in the body of the sample cell for schizolysis biological sample. In one aspect of the present invention, the height of the body of the sample tube is about 20-80mm, such as about 30-50 mm.
Fig. 3 is a cross-sectional view of an exemplary sample tube for lysing a biological sample provided by the present invention. Which is a cross-section through two symmetrical outer concave strips 22 of the body of the aforementioned sample tube as shown in figure 1. As shown in fig. 3, the inner wall of the tube body 2 of the sample tube provided by the present invention has at least two symmetrical axial strip-shaped protrusions 23. In other embodiments of the present invention, there may be a plurality of axial strip-like protrusions, such as 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 strips, etc. The plurality of axial strip-shaped bulges can be uniformly arranged on the inner wall of the pipe body. The height of the protrusions relative to the inner wall of the shaft is about 1-5mm, for example about 2 mm. The inventor of the utility model unexpectedly discovers that the inside of sample cell sets up strip protruding, when oscillating the sample cell, can make glass pearl or porcelain pearl change the direction of motion along the pipe wall motion in-process for the collision between glass pearl or porcelain pearl and the cell is more violent, can make the sample schizolysis more complete remarkably.
In one embodiment of the present invention, the sample tube for lysing a biological sample further contains abrasive particles. The abrasive particles are fine particles having a certain hardness, such as glass beads or ceramic beads. The abrasive particles may have a particle size of about 0.1-1 mm. In operation, the abrasive particles are vortexed with the biological sample to mechanically disrupt the biological sample.
The utility model provides a sample cell for schizolysis biological sample need vibrate at work. Various known oscillators capable of generating oscillation can be used for matching with the sample tube for cracking the biological sample. Fig. 4 is an exemplary working state diagram of the sample tube and the adapted oscillator for lysing biological samples according to the present invention. As shown in fig. 4, the oscillator 3 has an eccentric seat 31 for accommodating a syringe and a base 34, and the oscillator 3 rotates around the axis of the direct current 32 by using an eccentric shaft 33, so that the eccentric seat 21 generates high-speed vibration. The sample tube is placed in the eccentric seat, and the glass beads or the ceramic beads added into the sample tube move at high speed and vibrate, so that the purpose of breaking cells in the sample is achieved. The motor used in the device of the present invention and its rotation speed can be determined by a person skilled in the art according to the prior art or limited experiments.
The utility model provides a sample tube for cracking biological sample is through mode schizolysis sample of physics grinding, and it can also jointly use with the mode of other schizolysis cells. Other means of lysing cells include various means of chemical or enzymatic lysis. Wherein corresponding lysis reagents (including various reagents and/or solutions for lysing sample cells) can be added to the sample tube provided by the present invention to lyse the cells.
In one embodiment of the present invention, the other means of lysing the cells is chemical lysing agent lysis. The lysing agents that can be used include various surfactants such as SDS, Triton X, NP-40, etc., as well as other chemical agents such as buffers, protease inhibitors, reducing agents, etc., which serve the primary functions of: (1) disrupting the lipid bilayer using detergent, disrupting the cells; (2) dissolving the protein; (3) promoting protein denaturation; (4) inhibiting the activity of protease and nuclease.
In one embodiment of the present invention, the other means of lysing the cells is enzymatic lysis. The enzyme which can be used may be various enzymes which cleave components in cell walls or cell membranes, such as Labiase lyase, lysostaphin, hen egg protein-derived lysozyme, human-derived lysozyme, achromopeptidase, Streptomyces globisporus-derived mutanolysin, Staphylococcus aureus-derived alpha-hemolysin, chitinase, Rhizoctonia solani-derived lyase, Arthrobacter luteus-derived lyase, Trichoderma harzianum-derived lyase, Streptococcus pyogenes-derived pneumolysin O, tetanus bacillus-derived tetanus pneumolysin, and the like.
The utility model provides an above-mentioned sample cell for schizolysis biological sample is fit for being used for various automatic molecular in vitro diagnostic instruments. In one embodiment of the present invention, the molecular in-vitro diagnostic apparatus comprises a nucleic acid extraction and detection device, and a computer-based automatic control system. The nucleic acid extraction and detection device comprises a sample receiving module, a cracking module, a nucleic acid extraction module and a nucleic acid amplification module. The automatic system can control the liquid distribution module of the molecular in-vitro diagnostic instrument to transfer samples between or in the sample receiving module, the cracking module, the nucleic acid extraction module and the nucleic acid amplification module of the nucleic acid extraction and detection device in a programmed manner, and perform cracking, extraction, amplification, detection and other procedures according to preset steps under proper conditions to detect the samples to be detected. The utility model provides a sample cell for schizolysis biological sample diagnostic instrument can reach rather than supporting oscillator that uses can be used to diagnostic instrument's schizolysis module. The diagnostic instrument can be used for identifying infection sources, detecting genetic diseases, detecting cancers or detecting genetic variation.
In an embodiment of the present invention, there is also provided an external molecular diagnosis apparatus, which includes the aforementioned sample tube for lysing a biological sample, and an oscillator used in cooperation with the sample tube.
In one embodiment of the present invention, the diagnostic device may be used for the detection of: influenza virus, enterovirus, hepatitis B virus, hepatitis C virus, Ebola virus, Marburg virus, SARS virus, Securia virus, bunyavirus, rhinovirus, respiratory nucleocapsid virus, cholera virus, etc., or bacterial pathogens such as tubercle bacillus, Escherichia coli, Acinetobacter baumannii, Diplococcus pneumoniae, Streptococcus lactis, Sporosarcina urens, Staphylococcus aureus, Bacillus subtilis, Bacillus anthracis, Bacillus subtilis, Bacillus streptococci, Bacillus proteus, Vibrio cholerae, Treponema pallidum, etc.
In one embodiment of the invention, the diagnostic instrument may be used for the detection of: gastric cancer, liver cancer, lung cancer, esophageal cancer, cervical cancer, breast cancer, colon cancer, rectal cancer, nasopharyngeal cancer, ovarian cancer, renal cancer, bladder cancer, thyroid cancer, skin cancer, etc.; malignant tumors derived from mesenchymal tissues such as muscle, fat, bone, blood vessel, lymph, etc., for example, rhabdomyosarcoma, leiomyosarcoma, fibrosarcoma, liposarcoma, osteosarcoma, chondrosarcoma, angiosarcoma, lymphosarcoma, etc. Also, for example, leukemia, Hodgkin's disease, Wilms ' tumor (nephroblastoma), melanoma, retinoblastoma, seminoma, granuloma, Kupffer's tumor, Ewing's tumor, malignant vascular endothelial cell tumor, or Paget's disease of the breast.
Although the present invention has been described in detail with respect to the exemplary embodiments and advantages thereof, it should be understood that various changes, substitutions, and alterations can be made hereto without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (8)
1. The utility model provides a sample cell for schizolysis biological sample, its includes tube cap and body, its characterized in that, the inner wall of the body of sample cell has two at least strip projections, the relative body inner wall's of body height of strip projection is 1-5mm, wherein, the sample cell contains the abrasive particle.
2. The sample tube of claim 1, wherein the tube cap has a hollow tube cap with an outer membrane on an upper portion and an inner membrane on a lower portion.
3. The sample tube of claim 2, wherein the inner membrane has a fracture to facilitate insertion of a tubular object through the fracture into the inner membrane.
4. The sample tube of claim 2, wherein the inner membrane is made of rubber or silicone.
5. The sample tube of claim 2, wherein the outer membrane is an aluminum plastic or plastic-encapsulated membrane.
6. The sample tube of claim 1, wherein the height of the strip-shaped protrusion relative to the inner wall of the tube body is 2 mm.
7. The sample tube of claim 1, wherein the abrasive particles have a particle size of 0.1-1 mm.
8. An in vitro molecular diagnostic instrument comprising a nucleic acid extraction and detection device comprising a sample receiving module, a lysis module, a nucleic acid extraction module, a nucleic acid amplification module, and a liquid distribution module, and a computer-based automated control system,
characterized in that the lysis module of the nucleic acid extraction and detection device comprises a sample tube for lysing a biological sample according to any one of claims 1 to 7, and a vibrator used in conjunction therewith.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202020511549.8U CN213327606U (en) | 2020-04-09 | 2020-04-09 | Sample tube for cracking biological sample and molecular in-vitro diagnostic instrument |
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| CN202020511549.8U CN213327606U (en) | 2020-04-09 | 2020-04-09 | Sample tube for cracking biological sample and molecular in-vitro diagnostic instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115138413A (en) * | 2022-08-02 | 2022-10-04 | 石家庄元鼎医疗器械有限公司 | Puncture type ureter convenient to sample |
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2020
- 2020-04-09 CN CN202020511549.8U patent/CN213327606U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115138413A (en) * | 2022-08-02 | 2022-10-04 | 石家庄元鼎医疗器械有限公司 | Puncture type ureter convenient to sample |
| CN115138413B (en) * | 2022-08-02 | 2023-09-22 | 石家庄元鼎医疗器械有限公司 | Puncture type urinary catheter convenient for sampling |
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