CN213327539U

CN213327539U - Magnetic needle and cannula system and apparatus for extracting biologically active substances

Info

Publication number: CN213327539U
Application number: CN202020713122.6U
Authority: CN
Inventors: 赵海峰; 江楠
Original assignee: Medshin International Co ltd
Current assignee: Medshin International Co ltd
Priority date: 2020-04-30
Filing date: 2020-04-30
Publication date: 2021-06-01
Anticipated expiration: 2030-04-30

Abstract

The utility model provides a magnetic needle and sleeve pipe system for extracting bioactive substance, which comprises: the device comprises a magnetic needle group, a sleeve group and a sleeve rotating mechanism, wherein the sleeve rotating mechanism is provided with a belt, a transmission rod group and a belt driving mechanism, the transmission rod group comprises a plurality of transmission rods, and the belt is sequentially in sliding contact with each transmission rod; the sleeve is connected with the transmission rod, and when the belt moves, the transmission rod drives the sleeve to rotate around the axis of the sleeve. The utility model also provides an instrument for extracting bioactive substances by using the magnetic needle and the sleeve system.

Description

Magnetic needle and cannula system and apparatus for extracting biologically active substances

Technical Field

The utility model relates to a medical science and biological detecting instrument field. Specifically, the utility model provides a magnetic needle and sleeve system for extracting bioactive substances by a magnetic rod method, and an instrument using the system.

Background

In the fields of clinical disease diagnosis and molecular biology research, extraction of bioactive substances such as proteins and nucleic acids plays an important role. Nucleic acids are the primary material basis for the storage, replication, and transmission of genetic information. The development of biotechnology is changing day by day, and with the application of PCR technology in various fields, including medical disease detection, agricultural transgene detection and other applications in many aspects, high-throughput methods and instruments for extracting bioactive substances from samples and fully-automatic extraction of bioactive substances are required.

Magnetic rod extraction is a technology developed in recent years, and the principle of the technology is that a magnetic separation device is immersed into liquid, and magnetically attracted particles in a mixture are concentrated on the surface of the separation device through the action of a magnetic field. In one mode, the magnetic separation device is a sleeve, the sleeve is made to generate magnetism by inserting a magnetic rod, particles which can be attracted by magnetism in the mixture are attracted to the surface of the sleeve, when the magnetic rod is pulled away, the sleeve loses magnetism, and the particles which are attracted by magnetism are separated from the sleeve. The existing nucleic acid extraction instrument usually adopts a magnetic bar oscillation mode: the mechanism driving the magnetic bar can drive the magnetic bar to move up and down in the sleeve, which is beneficial to cracking, rinsing and purifying in the extraction process.

However, the existing nucleic acid extraction apparatus and method have the disadvantages of complicated operation mechanism, large abrasion of mechanical devices, difficult maintenance and short service life.

SUMMERY OF THE UTILITY MODEL

The utility model provides a magnetic needle and bushing system for bioactive substance extraction appearance of bar magnet method still provides the use bioactive substance extraction appearance of system. The system and the extractor of the utility model can be applied in various fields such as clinical disease diagnosis, blood transfusion safety, forensic medicine identification, environmental microorganism detection, food safety detection, molecular biology research and the like, and are used for extracting and purifying bioactive substances such as cells, proteins and nucleic acids.

Specifically, the utility model provides a magnetic needle and bushing system for bioactive substance draws appearance, wherein magnetic needle and bushing system include:

a magnetic needle group, wherein the magnetic needle of the magnetic needle group can be vertically inserted into and separated from a sleeve arranged below the magnetic needle group,

the sleeve of the sleeve group is a hollow rod body capable of accommodating the magnetic needle and can be vertically inserted into and separated from the sample processing area arranged below the sleeve; the sleeve rotating mechanism is provided with a belt, a transmission rod group and a belt driving mechanism, wherein the transmission rod group comprises a plurality of transmission rods, the transmission rods are arranged in a transmission rod row or a plurality of parallel transmission rod rows, and the belt is sequentially in sliding contact with each transmission rod; the sleeve is connected with a transmission rod of the sleeve rotating mechanism, and when the belt moves, the transmission rod drives the sleeve to rotate around the axis of the sleeve.

In one aspect of the present invention, the body of the sleeve has a hollow lumen and a closed bottom, and the upper portion thereof has a connecting portion for engaging with the shaft of the transmission rod.

In yet another aspect of the present invention, the top of the drive rod has an opening and a hollow rod body; the magnetic needle can thus be inserted into and through the transmission rod, protruding into a sleeve connected to the transmission rod. The transmission rod is also provided with a bearing, and the rod body rotates along with the inner ring of the bearing.

In one aspect of the present invention, in the magnetic needle and sleeve system, the belt of the sleeve rotating mechanism is sequentially brought into sliding contact with each transmission rod in a staggered winding manner.

In one aspect of the present invention, the magnetic needle and the sleeve system, wherein the transmission rod of the sleeve rotating mechanism or each of the transmission rod rows of the plurality of transmission rod rows is contacted with a belt in a staggered winding manner. In another aspect of the present invention, the transmission rod row of the sleeve rotating mechanism further includes an auxiliary rotating rod, and the belt contacts the transmission rod row in a staggered winding manner and changes the direction after contacting the auxiliary rotating rod.

In one aspect of the present invention, the magnetic needle and the sleeve system, wherein the transmission rods of every two transmission rod rows in the plurality of rotation rod rows of the sleeve rotating mechanism contact with one belt in a staggered winding manner.

In the magnetic needle and cannula system provided by the present invention, the belt driving mechanism of the cannula rotating mechanism can adopt various driving mechanisms known in the art. In one embodiment of the present invention, the belt driving mechanism of the sleeve rotating mechanism is a motor: the belt is directly driven by the motor rotating shaft. Preferably, each belt is driven by a motor.

In one aspect of the present invention, the magnetic needle and the sleeve system, wherein the belt driving mechanism of the sleeve rotating mechanism is a belt driving mechanism. The utility model discloses an wherein in another aspect, sleeve pipe slewing mechanism's belt drive mechanism includes driving motor, action wheel, drive belt, goes up from driving wheel, actuating lever, follows the driving wheel down, wherein driving motor drive action wheel takes place to rotate, makes to go up from driving wheel, actuating lever and takes place to rotate from driving wheel down through the drive belt, and the belt is connected from driving wheel gliding property down, from this time down takes place to rotate from the driving wheel, makes the belt produce the motion through frictional force. The belt driving mechanism can comprise one or more upper driven wheels, driving rods and lower driven wheels, and drives one or more corresponding belts; wherein the driving belt and the plurality of upper driven wheels may be in contact in a staggered winding manner.

In one aspect of the present invention, the magnetic needle and sleeve system has a sleeve platform for supporting the sleeve rotating mechanism, which includes a transmission rod set platform, each transmission rod of the transmission rod set passes through the transmission rod set platform, and is fixed on the transmission rod set platform. In yet another aspect of the present invention, the casing platform further comprises a belt drive mechanism platform; the belt driving mechanism is arranged on the belt driving mechanism platform.

The utility model also provides a method of using aforementioned magnetic needle and sleeve pipe system to drive magnetic needle and sleeve pipe in bar magnet method bioactive substance extraction appearance, wherein magnetic needle and sleeve pipe system include magnetic needle group and thimble group and sleeve pipe slewing mechanism in the method, the magnetic needle can vertically insert and break away from the sleeve pipe that sets up below it, and the sleeve pipe can vertically insert and break away from the sample processing district that sets up below it for the hollow stick body that can hold the magnetic needle, the sleeve pipe slewing mechanism can drive the sleeve pipe and take place the rotation around its axle center, stirs the liquid sample in sample processing district. The sleeve rotating mechanism is provided with a belt, a transmission rod group and a belt driving mechanism, wherein the transmission rod group comprises a plurality of transmission rods, the transmission rods are arranged in a transmission rod row or a plurality of parallel transmission rod rows, the belt is in sliding contact with each transmission rod in sequence, the sleeve is connected with the transmission rods of the sleeve rotating mechanism, and when the belt moves, the sleeve is driven to rotate around the axis of the sleeve through the transmission rods.

The utility model also provides an instrument for drawing bioactive substance, including shell, workstation, sample processing district has on it, as before the utility model discloses a magnetism needle and sleeve pipe mobile system, it includes magnetism needle group, sleeve pipe group and sleeve pipe slewing mechanism to and magnetism needle elevating system, sleeve pipe elevating system and support magnetism needle elevating system and sleeve pipe elevating system's support. The sleeve rotating mechanism is provided with a belt, a transmission rod group and a belt driving mechanism, wherein the transmission rod group comprises a plurality of transmission rods, the transmission rods are arranged in a transmission rod row or a plurality of parallel transmission rod rows, the belt is in sliding contact with each transmission rod in sequence, the sleeve is connected with the transmission rods of the sleeve rotating mechanism, and when the belt moves, the sleeve is driven to rotate around the axis of the sleeve through the transmission rods.

In one aspect of the present invention, the magnetic needle lifting mechanism controls the magnetic needle group to ascend or descend. The magnetic needle lifting mechanism may employ various driving mechanisms known in the art. In one embodiment of the present invention, the magnetic needle lifting mechanism adopts a motor-screw mechanism, which may include, for example, a magnetic needle lifting motor and a magnetic needle lifting screw mechanism driven thereby, and has a magnetic needle group connecting plate for connecting and fixing with the magnetic needle group; the magnetic needle lifting motor drives the magnetic needle lifting screw rod mechanism to control the lifting of the magnetic needle group connecting plate, so that the lifting and descending of the magnetic needle group are controlled.

In one aspect of the present invention, the sleeve lifting mechanism controls the lifting or lowering of the sleeve group. The casing lifting mechanism may employ various drive mechanisms known in the art. In one embodiment of the present invention, the magnetic needle lifting mechanism adopts a motor-screw mechanism, which may include, for example, a casing lifting motor and a casing lifting screw mechanism driven thereby, and has a casing group connection board for connecting and fixing with a casing group; the sleeve lifting motor drives the sleeve lifting screw rod mechanism to control the lifting of the sleeve group connecting plate, so that the lifting and descending of the sleeve group are controlled.

The system and apparatus of the present invention can be used to extract and purify biologically active substances.

The systems and apparatus of the present invention described above may be used to extract biologically active substances, such as cells, proteins or nucleic acids.

The instrument of the present invention may be an instrument for extracting and purifying nucleic acid.

The apparatus of the present invention may be an apparatus suitable for point-of-care testing (POCT).

Drawings

Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments made with reference to the following drawings:

fig. 1 is a first directional perspective view of a magnetic needle and cannula system in accordance with a preferred embodiment of the present invention.

Fig. 2 is a schematic view of a connection structure of a sleeve and a sleeve rotating mechanism of a magnetic needle and sleeve system according to a preferred embodiment of the present invention, wherein the connection mode and the transmission relationship between a transmission rod and the sleeve in the sleeve rotating mechanism and the connection relationship between all or part of the transmission rods in a transmission rod set and a belt are exemplarily shown. Wherein FIG. 2A is a first directional perspective view and FIG. 2B is a top view; wherein the entire drive link group comprises 96 drive links in 8 drive link rows, 24 drive links of two of the drive link rows are shown; fig. 2C is a top view of another exemplary bushing and bushing rotation mechanism, wherein the set of drive links consists of 12 drive links in 1 drive link row.

Fig. 3 is a perspective view of the combination of the sleeve of the magnetic needle and sleeve system and the drive rod of the sleeve rotation mechanism of the preferred embodiment of the present invention. Wherein fig. 3A is a first-direction perspective view and fig. 3B is a second-direction perspective view.

Fig. 4 is a partial perspective view of an exemplary apparatus for extracting bioactive substances provided by the present invention.

Detailed Description

For a better understanding and explanation of the present invention, the present invention will be described in further detail below with reference to the accompanying drawings.

Magnetic needle and cannula systemSystem

Fig. 1 is a first directional perspective view of a magnetic needle and cannula system in accordance with a preferred embodiment of the present invention. The magnetic needle and cannula system provided by the utility model can be used for extracting bioactive substances such as nucleic acid by adopting a magnetic needle method.

As shown in fig. 1, the magnetic needle and cannula system provided by the present invention comprises a magnetic needle set 3, a cannula set 1 and a cannula rotating mechanism 2.

The utility model provides a thimble group of magnetic needle and sleeve pipe system contains a plurality of sleeves. The number of cannulas comprised in the set of cannulas can be set as desired, typically ranging from 4 to about 200, such as 24, 48, 96, 128. The sleeves are typically arranged in several rows of sleeves arranged in parallel, wherein each row of sleeves has more than 1 sleeve arranged in a line, for example 4-20 sleeves per row of sleeves. In the preferred embodiment shown in fig. 1, the set of ferrules has 8 rows of ferrules, 12 ferrules in each row, for a total of 96 ferrules.

The cannula is a tube having a closed bottom and a hollow lumen. The sleeve may have, for example, an elongated cylindrical shape. The material of the sleeve is typically non-magnetic. The material and thickness are chosen and designed such that they do not weaken the magnetic field or only slightly influence it, but it is ensured that a magnetic field capable of attracting magnetic beads or biological samples with magnetic beads is generated outside the cannula when the magnetic needle is inserted. The sleeve is capable of vertical insertion into and removal from a sample processing region disposed therebelow.

The sample processing region can include a working region, a sample well, or a combination thereof. The sample processing area is used for arranging sample grooves or sample holes for processing samples, or is used for placing vessels for loading samples, such as single or single-row centrifuge tubes, standard culture plates such as 24-well plates or 96-well plates, and the required non-standard culture plates can also be placed in the sample processing area. In the embodiment shown in FIG. 1, the sample processing region comprises a culture plate 4 having wells 41 corresponding to the number of cannulae in the set. The sleeve can be inserted into the well of the culture plate and rotated in the well, and the solution contained in the culture plate is stirred.

The magnetic needle group 3 has a plurality of magnetic needles 31 corresponding to the arrangement and number of the cannulas of the cannula group 1. The magnetic needle can be inserted into and detached from the cannula vertically. The bottom end of the magnetic needle (i.e., the end inserted into the cannula) is magnetic. When the magnetic needle is inserted into and contacts or approaches the bottom of the sleeve, a magnetic field can be generated outside the sleeve, so that the sleeve can adsorb and separate magnetic beads or biological samples with the magnetic beads in a solution outside the sleeve. In this embodiment, the bottom of the magnetic needle has a permanent magnet, and thus is magnetic. In other embodiments of the present invention, the magnetic needle may generate magnetism by electromagnetic action (electromagnet).

In the magnetic needle and cannula system provided by the present invention, the cannula can be inserted into the hole of the plate and can be rotated in the hole to stir the solution contained in the plate. The rotation of the sleeve is effected by means of the sleeve rotation mechanism 2. The sleeve rotating mechanism is provided with a belt 21, a transmission rod group 22 and a belt driving mechanism 23, wherein the transmission rod group is provided with a plurality of transmission rods 221 corresponding to the arrangement mode and the number of the sleeves of the sleeve group 1. The belt is in sliding contact with each transmission rod in sequence, and the sleeve is connected with the transmission rod of the sleeve rotating mechanism. When the belt moves under the driving of the belt driving mechanism, each transmission rod is driven to rotate through friction, so that the sleeve connected with the transmission rod rotates relative to the axis.

The utility model provides an among magnetic needle and the sleeve pipe system, the drive link group among the sleeve pipe slewing mechanism includes a plurality of transfer lines, the transfer line sets up the arrangement mode that the parallel transfer line of a transfer line row or several was arranged. Wherein each row of drive rods contains more than 1 drive rod arranged in a line. There are typically 4-20 drive rods per drive rod, for example 8, 12 or 16. The transmission rods are not contacted with each other, so that mechanical noise, damage to parts and operation failure caused by mutual friction of the transmission rods during rotation are reduced.

In one embodiment of the present invention, the sleeve rotating mechanism has a row of transmission rods therein, and each transmission rod is in contact with a belt in a staggered winding manner.

In one embodiment of the invention, the sleeve rotation mechanism has several drive rod rows, preferably an even number of drive rod rows, for example two, four, eight or twelve drive rod rows. In one embodiment of the present invention, the driving rods of every two driving rod rows of the plurality of driving rod rows contact with one belt in a staggered winding manner. In another embodiment of the present invention, the drive rod of each of the plurality of drive rod rows contacts a belt in a staggered winding manner.

Fig. 3 is a perspective view of the combination of the sleeve of the magnetic needle and sleeve system and the drive rod of the sleeve rotation mechanism of the preferred embodiment of the present invention. Wherein fig. 3A is a first-direction perspective view and fig. 3B is a second-direction perspective view. As shown in fig. 3, the shaft 111 of the sleeve 11 has a hollow lumen and a closed bottom, the upper portion of which has a connection portion 112 for engaging with the lower portion 2213 of the shaft of the drive rod 221. The top of the drive rod 221 has an opening 2214 and a hollow shaft into and through which a magnetic needle can be inserted and extend into a cannula attached to the drive rod.

Fig. 2 is a schematic view of a connection structure of a sleeve and a sleeve rotating mechanism of a magnetic needle and sleeve system according to a preferred embodiment of the present invention, wherein the connection mode and the transmission relationship between a transmission rod and the sleeve in the sleeve rotating mechanism and the connection relationship between all or part of the transmission rods in a transmission rod set and a belt are exemplarily shown. Wherein FIG. 2A is a first directional perspective view and FIG. 2B is a top view; where the entire drive rod group comprises 96 drive rods for 8 drive rod rows, 24 drive rods for two of the drive rod rows are shown. Fig. 2C is a top view of another exemplary bushing and bushing rotation mechanism, wherein the set of drive rods consists of 12 drive rods in 1 drive rod row, and further comprising 1 auxiliary rotation rod.

In the preferred embodiment magnetic needle and cannula system shown in fig. 2, two rows of cannulae in the cannula set each have 12 cannulae 11, said two rows being arranged in parallel. Correspondingly, the drive rod set of the bushing-rotating mechanism shown in fig. 2 has two rows of 24 drive rods 221 (12 drive rods in each row). In the magnetic needle and cannula system provided by the present invention, the belt driving mechanism can drive a plurality of belts simultaneously, one of which is exemplarily shown in fig. 2, and the connection and transmission relationship thereof with the cannula of the two cannula rows.

As shown in fig. 2B, the belt 21 is sequentially in sliding contact with the upper portion 2211 of the shaft of each of the two rows of drive links in a staggered winding manner. The tensioned belt 21 exerts a pressure on the shaft of the transmission lever, whereby the movement of the belt 21 causes the rotation of the transmission lever by friction, thereby causing the rotation of the sleeve 11, fixed to the other end of the transmission lever, with respect to its axis.

In another embodiment of the present invention, the transmission rod of each transmission rod row of the transmission rod row or the plurality of transmission rod rows of the sleeve rotating mechanism contacts with a belt in a staggered winding manner, that is, the transmission rod of each transmission rod row is driven by a belt. As shown in fig. 2C, in which the transmission lever group is composed of 12 transmission levers in 1 transmission lever row, the belt 21 is sequentially contacted with each transmission lever of the transmission lever row in a staggered winding manner, and the belt direction is changed via the auxiliary transmission lever 25. The tensioned belt 21 exerts a pressure on the shaft of the transmission rod, whereby the movement of the belt 21 causes the rotation of the transmission rod by friction, thereby driving the rotation of the sleeve fixed to the other end of the transmission rod with respect to its axis.

In the magnetic needle and cannula system provided by the present invention, the belt driving mechanism of the cannula rotating mechanism can adopt various driving mechanisms known in the art. In one embodiment of the present invention, the belt driving mechanism of the sleeve rotating mechanism is a motor: the belt is directly driven by the motor rotating shaft. Wherein each belt may be driven by a motor.

In another embodiment of the present invention, the belt driving mechanism of the sleeve rotating mechanism is a belt transmission mechanism. As shown in fig. 2A, in the magnetic needle and cannula system of the present preferred embodiment, the belt driving mechanism 23 includes an upper driven pulley 231, a driving lever 233, a lower driven pulley 232, a driving motor 234, a driving pulley 235, and a driving belt 236. The driving motor 234 drives the driving pulley 235 to rotate, and the upper driven pulley 231, the driving lever 233 and the lower driven pulley 232 are rotated by the driving belt 236. The belt 21 is slidably connected to the lower driven pulley 232, and when the lower driven pulley 232 rotates, the belt 21 is moved by friction. The belt driving mechanism may include one or more of the upper driven wheel 231, the driving rod 233, and the lower driven wheel 232 to drive the corresponding one or more belts; wherein the driving belt 236 and the plurality of upper driven wheels may be in contact in a staggered winding manner.

As shown in fig. 1, the magnetic needle and cannula system of the preferred embodiment of the present invention has a cannula platform 24 for supporting the cannula rotational mechanism 2, which includes a drive rod set platform 241 and a belt drive mechanism platform 242. Each driving rod 221 of the driving rod group 22 passes through the driving rod group platform 241, and a shaft sleeve 2212 of a bearing of the driving rod group 22 is fixed on the driving rod group platform 241. The belt drive 22 is disposed on a belt drive platform 242.

Apparatus for extracting bioactive substances by magnetic needle method

The utility model also provides an instrument for extracting bioactive substances by adopting the magnetic acupuncture method, such as a nucleic acid extractor. The instrument includes shell, foretell the utility model provides a magnetic needle and sleeve pipe system, workstation (have sample processing district on it), magnetic needle elevating system, sleeve pipe elevating system and be used for supporting magnetic needle elevating system and sleeve pipe elevating system's support.

The utility model provides an apparatus for extracting bioactive substances has a shell (not shown in the figure). The housing may separate the instrument interior from the atmosphere to avoid sample contamination and part damage. The housing panels are typically made of a metallic material. The housing may have a sample window that can be opened and closed for placing and removing a sample. In addition, the housing may have an external device interface connected to an external device, a display screen displaying an operation interface, various manipulation switches, keys or buttons, and the like.

The instrument for extracting bioactive substances provided by the utility model is particularly suitable for point-of-care testing (POCT). The housing of the instrument of the present invention may also have a handle or armrest feature that facilitates grasping or lifting by a user.

The utility model provides an instrument for extracting bioactive substances is provided with a workbench, and a sample processing area is arranged on the workbench. The sample processing area is used for arranging sample grooves or sample holes for processing samples, or is used for placing vessels for loading samples, such as single or single-row centrifuge tubes, standard culture plates such as 24-well plates or 96-well plates, and the required non-standard culture plates can also be placed in the sample processing area.

The utility model provides an extract bioactive material's instrument adopts aforementioned the utility model discloses a magnetism needle and sleeve pipe system, it includes magnetism needle group 3, sleeve pipe group 1 and sleeve pipe slewing mechanism 2. Wherein the set of cannulas comprises a plurality of cannulas, for example 24, 48, 96, 128 cannulas. The sleeve is capable of vertical insertion into and removal from a sample processing region disposed therebelow. Wherein the magnetic needle set has a plurality of magnetic needles corresponding to the cannulas of the cannula set. The magnetic needle can be vertically inserted into and detached from a cannula disposed therebelow.

As shown in FIGS. 1 and 4, the apparatus of the present invention comprises a magnetic needle, a cannula and a culture plate placed in a sample processing area. In operation, magnetic needle 31 needs to be inserted into and removed from cannula 11, and cannula 11 needs to be inserted into or removed from well 41 of the plate, so that the magnetic needle and cannula move up or down, respectively. The casing platform 24 for supporting the casing rotating mechanism 2 includes a transmission rod set platform 241 and a belt driving mechanism platform 242. Each drive link 221 of the drive link assembly 22 extends through the drive link assembly platform 241 and is secured to the drive link assembly platform 241 at the bearing 2212.

The instrument for extracting bioactive substances provided by the utility model also comprises a magnetic needle lifting mechanism 5, which controls the rising or falling of the magnetic needle group. As shown in fig. 4, in the exemplary instrument, the needle elevating mechanism 5 includes a needle elevating motor 52 and a needle elevating screw mechanism 53 driven thereby, and has a needle group connecting plate 51 for connecting and fixing with the needle group 3: the needle elevating motor 52 drives the needle elevating screw mechanism 53 to control the elevation of the needle group connecting plate 51, thereby controlling the elevation and the descent of the needle group 3, i.e., the insertion or the removal of the sleeve disposed below the needle group. Similarly, the apparatus for extracting bioactive substances provided by the present invention further comprises a sleeve lifting mechanism 6, which comprises a sleeve lifting motor (not shown) and a sleeve lifting screw mechanism 63 driven by the sleeve lifting motor, and a magnetic needle set connecting plate 61 for connecting and fixing with the sleeve set 1.

The instrument for extracting bioactive substances provided by the utility model also comprises a bracket 7 for supporting the magnetic needle lifting mechanism and the sleeve lifting mechanism.

The utility model provides a magnetic needle and sleeve pipe system to and adopt the instrument of the bioactive substance that draws of shown magnetic needle and sleeve pipe system can provide various fluxes as required more conveniently, the degree of difficulty and the great reduction of complexity of its assembly and debugging. Additionally, the utility model discloses a magnetic needle and bushing system adopt belt mechanism to control a plurality of sheathed tube rotations and stir liquid sample, have reduced the sample pollution effectively, adopt the vibration mode to mix the sample pollution that leads to with the stirring to liquid sample among the especially prior art. The utility model provides a magnetism needle and bushing system to and adopt the instrument of the bioactive substance that draws of shown magnetism needle and bushing system still obviously than adopt other drive methods (for example rack and pinion mechanism) driven noises among the prior art little, reduced daily and long-term maintenance's the degree of difficulty, prolonged work duration and working life. The utility model provides a magnetic needle and bushing system to and the instrument that adopts shown magnetic needle and bushing system to draw bioactive substance has operating device simply, compact structure, small advantage, specially adapted point-of-care testing (POCT).

Moreover, the scope of the present application is not intended to be limited to the particular embodiments of the process, machine, manufacture, composition of matter, means, methods and steps described in the specification. As one of ordinary skill in the art will readily appreciate from the disclosure of the present invention, processes, machines, manufacture, compositions of matter, means, methods, or steps, presently existing or later to be developed, that perform substantially the same function or achieve substantially the same result as the corresponding embodiments described herein may be utilized according to the present invention. Accordingly, the appended claims are intended to include within their scope such processes, machines, manufacture, compositions of matter, means, methods, or steps.

Claims

1. A magnetic needle and cannula system for extracting a biologically active substance, wherein the magnetic needle and cannula system comprises:

the sleeve of the sleeve group is a hollow rod body capable of accommodating the magnetic needle and can be vertically inserted into and separated from the sample processing area arranged below the sleeve; the sleeve rotating mechanism is provided with a belt, a transmission rod group and a belt driving mechanism, wherein the transmission rod group comprises a plurality of transmission rods, the transmission rods are arranged in a transmission rod row or a plurality of parallel transmission rod rows, and the belt is sequentially in sliding contact with each transmission rod; the sleeve is provided with a connecting part used for being combined with the rod body of the transmission rod and is connected with the transmission rod of the sleeve rotating mechanism, and when the belt moves, the sleeve is driven by the transmission rod to rotate around the axis of the sleeve.

2. The magnetic needle and cannula system of claim 1, wherein the belt of the cannula rotation mechanism is in sliding contact with each drive link sequentially in an interleaved winding manner.

3. A magnetic needle and cannula system according to claim 2 wherein the drive rod of the drive rod row or each of the plurality of drive rod rows of the cannula rotation mechanism is in contact with a belt in an interlaced winding pattern.

4. The needle and cannula system of claim 3, wherein the row of drive links of the cannula rotation mechanism further comprises an auxiliary drive link, the belt contacting the drive links of the one row of drive links in an interlaced winding pattern and changing course upon contact with the auxiliary drive link.

5. The magnetic needle and cannula system of claim 2, wherein the drive links of each two of the plurality of drive link rows of the cannula rotation mechanism are in contact with a belt in an interlaced winding manner.

6. The magnetic needle and cannula system of claim 1, wherein the belt drive mechanism of the cannula rotational mechanism is a motor.

7. The magnetic needle and cannula system of claim 1, wherein the belt drive mechanism of the cannula rotational mechanism is a belt drive mechanism.

8. The needle and cannula system of claim 7, wherein the belt driving mechanism of the cannula rotating mechanism comprises a driving motor, a driving wheel, a transmission belt, an upper driven wheel, a driving rod and a lower driven wheel, wherein the driving motor drives the driving wheel to rotate, the driving wheel, the lower driven wheel and the upper driven wheel are rotated by the transmission belt, the belt is slidably connected with the lower driven wheel, and therefore when the lower driven wheel rotates, the belt is moved by friction.

9. The needle and cannula system of claim 1 having a cannula mount for supporting the cannula rotation mechanism comprising a drive rod set mount through which each drive rod of a drive rod set passes and is secured to the drive rod set mount.

10. An apparatus for extracting biologically active substances, characterized in that it comprises:

the outer shell is provided with a plurality of grooves,

a stage having a sample processing region thereon,

the magnetic needle and sleeve moving system according to any one of claims 1 to 9, which comprises a magnetic needle set, a sleeve set and a sleeve rotating mechanism, wherein the magnetic needles of the magnetic needle set can be vertically inserted into and removed from the sleeves arranged below the magnetic needles, the sleeves of the sleeve set are hollow rods capable of accommodating the magnetic needles and can be vertically inserted into and removed from the sample processing areas arranged below the sleeves, the sleeve rotating mechanism can drive the sleeves to rotate around the axes of the sleeves to stir the liquid samples in the sample processing areas, the sleeve rotating mechanism is provided with a belt, a transmission rod set and a belt driving mechanism, wherein the transmission rod set comprises a plurality of transmission rods, the transmission rods are arranged in a mode that one transmission rod row or a plurality of parallel transmission rod rows are arranged, the belt is sequentially in sliding contact with each transmission rod, the sleeves are connected with the transmission rods of the sleeve rotating mechanism, and when the belt moves, the sleeve is driven to rotate around the axis of the sleeve by the transmission rod,

the device comprises a magnetic needle lifting mechanism, a sleeve lifting mechanism and a bracket for supporting the magnetic needle lifting mechanism and the sleeve lifting mechanism.