CN213307096U

CN213307096U - Cooling device for cell cryopreservation

Info

Publication number: CN213307096U
Application number: CN202022082929.7U
Authority: CN
Inventors: 刘世勇; 余鹏程; 李雪野; 刘城城; 罗九干
Original assignee: Yunnan Heze Southwest Biological Science And Technology Co ltd
Current assignee: Yunnan Heze Southwest Biological Science And Technology Co ltd
Priority date: 2020-09-21
Filing date: 2020-09-21
Publication date: 2021-06-01
Anticipated expiration: 2030-09-21

Abstract

The utility model provides a heat sink of cell cryopreserving usefulness, including the cooling bucket, the cooling bucket comprises outer staving and interior staving, be equipped with the bounding wall between outer staving and the interior staving, divide into condensation chamber and liquid nitrogen chamber through the bounding wall between outer staving and the interior staving, the inboard in condensation chamber is equipped with the condensing coil, just be equipped with the heat preservation on the inner wall of outer staving, the heat preservation includes first stainless steel layer and second stainless steel layer, be equipped with the vacuum layer between first stainless steel layer outer wall and the second stainless steel layer inner wall, just the bottom of interior staving is equipped with the runner assembly. The utility model discloses make the liquid nitrogen can contact with interior staving uniformly, through interior cell of bucket body and frozen stock solution in the liquid nitrogen cooling, can effectual activity that keeps the cell, and reduced the influence of ambient temperature to the cooling bucket, be favorable to the activity that keeps the cell among the cooling process.

Description

Cooling device for cell cryopreservation

Technical Field

The utility model relates to a technical field that the cell freezes and deposits specifically is a heat sink of cell freezes usefulness.

Background

In the prior art, after cells are collected and processed, a freezing solution needs to be added to uniformly mix the cells and the freezing solution, and then the cells and the freezing solution are placed at an ultralow temperature, such as minus 80 ℃ or even lower minus 196 ℃, for storage, while stem cells need to be slowly cooled in the process of being cooled from room temperature to the ultralow temperature, but the existing freezing device only stores the stem cells at the low temperature in a freezer, so that the loss of cell activity is easily caused, and the failure probability in later use is increased.

According to patent document application number 201822049008.3, a heat sink that stem cell cold storage in-process used is provided, including shell, inner tube, refrigerant room and cooling room, the inside of shell is equipped with the inner tube, the top and the shell looks rigid coupling of inner tube, refrigerant room and cooling room are cut apart into with the shell to the inner tube, the liquid outlet has been seted up to the lower right corner of shell, be equipped with the second ball valve in the liquid outlet, gear and second commentaries on classics piece all with shell clearance fit, though make things convenient for the refrigerant to pour into the refrigerant room into, improved the efficiency that the refrigerant was changed, but external temperature can lead to the fact the influence to the cooling room, and then easily lead to the loss of cell activity, be unfavorable for the cryopreservation of cell.

SUMMERY OF THE UTILITY MODEL

The utility model mainly provides a cooling device for freezing and storing cells, which is used for solving the technical problem provided in the background technology.

The utility model provides a technical scheme that above-mentioned technical problem adopted does:

a cooling device for freezing and storing cells, which comprises a cooling barrel, wherein the cooling barrel consists of an outer barrel body and an inner barrel body, a surrounding plate is arranged between the outer barrel body and the inner barrel body, the space between the outer barrel body and the inner barrel body is divided into a condensation cavity and a liquid nitrogen cavity by the surrounding plate, a condensation coil pipe is arranged at the inner side of the condensation cavity, and the inner wall of the outer barrel body is provided with a heat-insulating layer, the heat-insulating layer comprises a first stainless steel layer and a second stainless steel layer, a vacuum layer is arranged between the outer wall of the first stainless steel layer and the inner wall of the second stainless steel layer, and the bottom of the inner barrel body is provided with a rotating assembly which comprises a rotating rod, the outer wall of the rotating rod is fixedly provided with a connecting sleeve, at least two connecting rods are uniformly distributed on the outer wall of the connecting sleeve along the circumference, a stirring rod is mounted at one end, far away from the connecting sleeve, of each connecting rod, and a plurality of propeller type stirring blades are mounted on the outer wall of each stirring rod side by side.

Preferably, the rotating assembly comprises a driving motor, the top end of the driving motor is fixed at the bottom of the outer barrel body, the top end of the rotating rod is fixedly connected with the bottom of the inner barrel body, and the bottom end of the rotating rod penetrates through the outer barrel body to be connected with an output shaft of the driving motor.

Preferably, a rotating part is installed at the joint of the rotating rod and the outer barrel body.

Preferably, a dustproof box is installed on the outer side of the driving motor, and the top opening of the dustproof box is fixedly connected with the bottom of the outer barrel body.

Preferably, the fixed parcel has the foam blanket on the outer wall on second stainless steel layer, the bonding is connected with the rock wool layer on the outer wall on foam blanket, be equipped with the dampproof course on the outer wall on rock wool layer.

Preferably, the coaming and the heat-insulating layer are both of hollow cylindrical structures, an exhaust tube is mounted at the top of the vacuum layer, and the top end of the exhaust tube penetrates through the outer barrel body and is connected with an air extractor through a pipeline.

Preferably, the water pipeline of the condensing coil pipe penetrates through the heat-insulating layer and the outer barrel body and extends to the outer side of the outer barrel body.

Preferably, a liquid inlet pipe is arranged above the liquid nitrogen cavity, and the top end of the liquid inlet pipe penetrates through the outer barrel body and extends to the outer side of the outer barrel body to be provided with a material conveying valve.

Compared with the prior art, the beneficial effects of the utility model are that:

the utility model stirs the liquid nitrogen through the dwang, the connecting sleeve, the connecting rod, the stirring rod and the propeller type stirring blade, so that the liquid nitrogen can be uniformly contacted with the inner barrel body, the cell and the frozen liquid in the inner barrel body can be effectively kept active through liquid nitrogen cooling, and meanwhile, the barrel body is cooled through the cooling water in the condensation coil pipe, and the vacuum layer is matched to reduce the influence of the external temperature on the cooling barrel, thereby being beneficial to keeping the activity of the cell in the cooling process; through driving motor and dwang, can drive the interior staving and rotate, can intensive mixing through the cell of pivoted interior staving messenger bucket and cryopreserved liquid, improved the mixed effect of cell and cryopreserved liquid.

The present invention will be explained in detail with reference to the drawings and specific embodiments.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a sectional view of the cooling barrel of the present invention;

FIG. 3 is a sectional view of the structure of the heat-insulating layer of the present invention;

FIG. 4 is an enlarged view of area A in FIG. 2;

fig. 5 is an enlarged view of region B in fig. 2.

In the figure: 1. a cooling barrel; 11. an outer barrel body; 12. an inner barrel body; 13. enclosing plates; 14. a condensation chamber; 15. a liquid nitrogen cavity; 16. a liquid inlet pipe; 161. a delivery valve; 2. a heat-insulating layer; 21. a first stainless steel layer; 22. a second stainless steel layer; 23. a vacuum layer; 24. a foam layer; 25. a rock wool layer; 26. a moisture barrier; 27. an air exhaust pipe; 3. a condenser coil; 31. a water delivery pipeline; 4. a dust-proof box; 5. a rotating assembly; 51. rotating the rod; 511. a rotating member; 52. a drive motor; 53. a connecting sleeve; 54. a connecting rod; 55. a stirring rod; 56. propeller type stirring blades.

Detailed Description

In order to facilitate understanding of the present invention, the present invention will be described more fully with reference to the accompanying drawings, in which several embodiments of the present invention are shown, but the present invention can be implemented in different forms, and is not limited to the embodiments described in the text, but rather, these embodiments are provided to make the disclosure of the present invention more thorough and comprehensive.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may be present, and when an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present, as the terms "vertical", "horizontal", "left", "right" and the like are used herein for descriptive purposes only.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs, and the use of the term knowledge in the specification of the present invention is for the purpose of describing particular embodiments and is not intended to limit the present invention, and the term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.

In the embodiment, referring to fig. 1-3 and 5, a cooling device for cell cryopreservation comprises a cooling barrel 1, wherein the cooling barrel 1 is composed of an outer barrel body 11 and an inner barrel body 12, a surrounding plate 13 is arranged between the outer barrel body 11 and the inner barrel body 12, the upper end and the lower end of the surrounding plate 13 can be connected with the top plate and the bottom plate of the outer barrel body 11 in a welding and fixing manner, the outer barrel body 11 and the inner barrel body 12 are divided into a condensation chamber 14 and a liquid nitrogen chamber 15 through the surrounding plate 13, the inner side of the condensation chamber 14 is provided with a condensation coil 3, the inner wall of the outer barrel body 11 is provided with a heat insulation layer 2, the heat insulation layer 2 comprises a first stainless steel layer 21 and a second stainless steel layer 22, the first stainless steel layer 21 and the second stainless steel layer 22 are both made of stainless steel plates, a vacuum layer 23 is arranged between the outer wall of the first stainless steel layer 21 and the inner wall of the second stainless steel layer 22, and the bottom of the inner barrel body, the rotating assembly 5 comprises a rotating rod 51, a connecting sleeve 53 is fixedly mounted on the outer wall of the rotating rod 51, at least two connecting rods 54 are uniformly distributed on the outer wall of the connecting sleeve 53 along the circumference, each connecting rod 54 is far away from one end of the connecting sleeve 53 and is provided with a stirring rod 55, each stirring rod 55 is provided with a plurality of propeller type stirring blades 56 on the outer wall side by side, liquid nitrogen is injected into the liquid nitrogen cavity 15, the rotating rod 51 drives the connecting sleeve 53 to rotate, the connecting rod 54 is driven to rotate by the connecting sleeve 53, the stirring rod 55 is driven to rotate by the connecting rod 54, the propeller type stirring blades 56 are driven to rotate by the stirring rod 55, the liquid nitrogen is stirred by the propeller type stirring blades 56, the liquid nitrogen can be uniformly contacted with the inner barrel body 12, cells and frozen stock solution in the inner barrel body 12 are cooled by the liquid nitrogen, and the activity of the cells can be, meanwhile, the barrel body is cooled by cooling water in the condensing coil 3, the vacuum layer 23 is matched to reduce the influence of the external temperature on the cooling barrel 1, the cooling process is facilitated to keep the activity of cells, the outer wall of the second stainless steel layer 22 is fixedly wrapped with the foam layer 24, the outer wall of the foam layer 24 is bonded with the rock wool layer 25, the outer wall of the rock wool layer 25 is provided with the moisture-proof layer 26, the heat preservation effect is improved through the foam layer 24 and the rock wool layer 25, the cooling process is facilitated to keep the activity of the cells, the moisture-proof layer 26 is used for reducing the damp damage of the foam layer 24 and the rock wool layer 25, the moisture-proof layer 26 can be made of moisture-proof paint such as water emulsion type moisture-proof paint, the coaming 13 and the heat preservation 2 are of hollow tubular structures, the top of the vacuum layer 23 is provided with the exhaust tube 27, the top end of the exhaust tube 27 penetrates through the outer barrel body 11 and, air exhaust device can adopt the aspiration pump, takes the air in vacuum layer 23 out through the aspiration pump, and vacuum layer 23 seals through first stainless steel layer 21 or second stainless steel layer 22, condensing coil 3's conduit 31 runs through heat preservation 2 and the outside that outer staving 11 extended to outer staving 11, and circulating water pump can be connected to conduit 31, and circulating water pump's the end of intaking is connected with coolant tank or cold water machine through the pipeline, provides the cooling water for condensing coil 3, the top in liquid nitrogen chamber 15 is equipped with feed liquor pipe 16, the top of feed liquor pipe 16 runs through outer staving 11 and extends to its outside and install conveying valve 161, and feed liquor pipe 16 accessible pipeline is connected with liquid nitrogen generator or liquid nitrogen tank, opens conveying valve 161 and can pour into the liquid nitrogen into in the liquid nitrogen chamber 15.

In an embodiment, please refer to fig. 1, 2 and 4, the rotating assembly 5 includes a driving motor 52, the top end of the driving motor 52 is fixed at the bottom of the outer barrel body 11, and the top end of the rotating rod 51 is fixedly connected with the bottom of the inner barrel body 12, the driving motor 52 rotates forward and backward, the rotating rod 51 is driven to rotate, the rotating rod 51 drives the inner barrel body 12 to rotate, the cells in the barrel can be fully mixed with the freezing solution through the rotating inner barrel body 12, the mixing effect of the cells and the freezing solution is improved, the bottom end of the rotating rod 51 penetrates through the outer barrel body 11 and is connected with the output shaft of the driving motor 52, a rotating part 511 is installed at the connection position of the rotating rod 51 and the outer barrel body 11, the rotating part 511 can adopt a rotating bearing, the dust-proof box 4 is installed at the outer side of the driving motor 52, the top opening of the dust-proof box, the dust and water prevention of the driving motor 52 is realized by the dust box 4.

The utility model discloses a concrete operation as follows:

firstly, injecting cells and frozen stock solution into the inner barrel body 12, opening the material delivery valve 161, injecting liquid nitrogen into the liquid nitrogen cavity 15 through the liquid inlet pipe 16, rotating forward and backward through the driving motor 52 to drive the rotating rod 51 to rotate, driving the connecting sleeve 53 to rotate through the rotating rod 51, driving the connecting rod 54 to rotate through the connecting sleeve 53, driving the stirring rod 55 to rotate through the connecting rod 54, driving the propeller type stirring blades 56 to rotate through the stirring rod 55, stirring the liquid nitrogen through the propeller type stirring blades 56, enabling the liquid nitrogen to be uniformly contacted with the inner barrel body 12, cooling the cells and the frozen stock solution in the inner barrel body 12 through the liquid nitrogen, simultaneously cooling the barrel body through cooling water in the condensing coil pipe 3, reducing the influence of the external temperature on the cooling barrel 1 by matching with the vacuum layer 23, being beneficial to keeping the activity of the cells in the cooling process, simultaneously driving the inner barrel body 12 to rotate through the rotating rod 51, enabling the cells in the barrel to be fully mixed with the frozen stock solution through the, the mixing effect of the cells and the frozen stock solution is improved.

The present invention has been described above with reference to the accompanying drawings, and it is obvious that the present invention is not limited by the above-mentioned manner, if the method and the technical solution of the present invention are adopted, the present invention can be directly applied to other occasions without substantial improvement, and the present invention is within the protection scope of the present invention.

Claims

1. The utility model provides a heat sink of usefulness is deposited to cell freezing, includes cooling bucket (1), its characterized in that, cooling bucket (1) comprises outer staving (11) and inner tank body (12), be equipped with bounding wall (13) between outer staving (11) and inner tank body (12), divide into condensation chamber (14) and liquid nitrogen chamber (15) through bounding wall (13) between outer staving (11) and inner tank body (12), the inboard of condensation chamber (14) is equipped with condenser coil (3), just be equipped with heat preservation (2) on the inner wall of outer staving (11), heat preservation (2) include first stainless steel layer (21) and second stainless steel layer (22), be equipped with vacuum layer (23) between first stainless steel layer (21) outer wall and second stainless steel layer (22) inner wall, just the bottom of inner tank body (12) is equipped with runner assembly (5), runner assembly (5) include dwang (51), fixed mounting has connecting sleeve (53) on the outer wall of dwang (51), there are two at least connecting rods (54), every along the circumference equipartition on the outer wall of connecting sleeve (53) connecting rod (54) keep away from the one end of connecting sleeve (53) and all install puddler (55), every all install a plurality of propeller type stirring leaves (56) side by side on the outer wall of puddler (55).

2. The cooling device for cell cryopreservation as claimed in claim 1, wherein the rotating assembly (5) comprises a driving motor (52), the top end of the driving motor (52) is fixed at the bottom of the outer barrel body (11), the top end of the rotating rod (51) is fixedly connected with the bottom of the inner barrel body (12), and the bottom end of the rotating rod (51) penetrates through the outer barrel body (11) and is connected with an output shaft of the driving motor (52).

3. The cooling device for freezing cells according to claim 2, wherein the rotating rod (51) is connected with the outer barrel (11) by a rotating member (511).

4. The cooling device for cell cryopreservation as claimed in claim 3, wherein a dust-proof box (4) is installed outside the driving motor (52), and an opening at the top of the dust-proof box (4) is fixedly connected with the bottom of the outer barrel body (11).

5. The cooling device for cell cryopreservation as claimed in claim 1, wherein the outer wall of the second stainless steel layer (22) is fixedly wrapped with a foam layer (24), the outer wall of the foam layer (24) is bonded with a rock wool layer (25), and the outer wall of the rock wool layer (25) is provided with a moisture-proof layer (26).

6. The cooling device for cell cryopreservation according to claim 1, wherein the enclosing plate (13) and the insulating layer (2) are both hollow cylindrical structures, an air suction pipe (27) is installed at the top of the vacuum layer (23), and the top end of the air suction pipe (27) penetrates through the outer barrel body (11) and is connected with an air suction device through a pipeline.

7. The cooling device for cell cryopreservation as claimed in claim 1, wherein the water pipe (31) of the condensation coil (3) extends to the outside of the outer barrel body (11) through the insulating layer (2) and the outer barrel body (11).

8. The cooling device for freezing and storing cells as claimed in claim 1, wherein a liquid inlet pipe (16) is disposed above the liquid nitrogen chamber (15), and a top end of the liquid inlet pipe (16) extends through the outer tub (11) to an outside of the outer tub and is provided with a material delivery valve (161).