CN213302112U - Ultraviolet-visible light detector for liquid chromatography - Google Patents

Abstract

The utility model belongs to the technical field of liquid chromatogram analysis, especially, relate to liquid chromatogram's ultraviolet-visible light detector, the power distribution box comprises a main box body, install radiator fan in the power distribution box body, light source device, the M1 speculum, the slit board, the M2 speculum, the grating, the M3 speculum, the flow-through cell, photodiode group, radiator fan sets up towards light source device, the slit board rotates the setting through first motor, first slit and second slit have been seted up on the slit board, the light filter is installed to second slit below, the grating rotates the setting, light source device's light path is in proper order through the M1 speculum, first slit or second slit, the M2 speculum, the grating, M3 speculum and flow-through cell shine to photodiode group on. The utility model discloses a set up cooling fan and cool down the heat reduction to light source device, maintain liquid chromatography system's stability, be provided with the light filter on the second slit, realize the wavelength calibration of detector, improve the accuracy nature that liquid chromatography system detected on the whole.

Description

Ultraviolet-visible light detector for liquid chromatography

Technical Field

The utility model belongs to the technical field of liquid chromatogram analysis, especially, relate to liquid chromatogram's ultraviolet-visible light detector.

Background

The system of the high performance liquid chromatograph consists of a liquid storage device, a pump, a sample injector, a chromatographic column, a detector, a recorder and the like. The mobile phase in the liquid storage device is pumped into the system by a high pressure pump, the sample solution enters the mobile phase through the sample injector and is loaded into a chromatographic column (stationary phase) by the mobile phase, and because each component in the sample solution has different distribution coefficients in two phases, when the two phases do relative motion, through repeated adsorption-desorption distribution processes, each component generates a large difference in moving speed, is separated into single components and sequentially flows out of the column, when the sample solution passes through the detector, the concentration of the sample is converted into an electric signal and is transmitted to a recorder, and data is printed in a map form.

Most common organic substances and part of inorganic substances have ultraviolet or visible light absorption groups and thus have stronger ultraviolet or visible light absorption capacity, and the ultraviolet-visible light detector of the liquid chromatograph is based on the principle, and the content of each component in the sample flowing through the flow cell is determined by measuring the absorption value of the sample to light with a specific wavelength. In the detection of the liquid chromatograph, the stability of each component needs to be ensured, the light source of the ultraviolet-visible light detector generates heat, and an active heat dissipation system is not provided, so that the stability of the liquid chromatograph system is influenced, the detection result is influenced, and finally the detection result of the product is inaccurate.

SUMMERY OF THE UTILITY MODEL

To the not enough of above-mentioned prior art, the utility model provides a liquid chromatogram's ultraviolet-visible light detector, the purpose is in order to solve among the prior art ultraviolet-visible light detector's light source production of heat to influence liquid chromatogram system's stability, cause the unsafe technical problem of testing result of product.

The utility model provides a liquid chromatogram's ultraviolet-visible light detector, concrete technical scheme is as follows:

the ultraviolet-visible light detector of the liquid chromatogram comprises a main box body which is a dark box, the main box body is internally provided with a heat radiation fan, a light source device, an M1 reflector, a slit plate, an M2 reflector, a grating, an M3 reflector, a flow cell and a photodiode group, the heat radiation fan is arranged towards the light source device, the slit plate is rotationally arranged through a first motor, the slit plate is provided with a first slit and a second slit, an optical filter is arranged below the second slit, the first slit or the second slit is aligned with the focus of the M1 mirror and the focus of the M2 mirror, the grating is connected with a second motor through a rotating shaft, and the light path of the light source device sequentially passes through the M1 reflector, the first slit or the second slit, the M2 reflector, the grating, the M3 reflector and the flow cell to irradiate the photodiode group.

In some embodiments, the light source device includes a darkroom box, a light source outlet is arranged at one side of the darkroom box, a quartz lens is installed on the light source outlet, a tungsten lamp, a lens and a deuterium lamp are fixedly installed in the darkroom box, the lens is arranged between the tungsten lamp and the deuterium lamp, and the tungsten lamp, a central point of the lens, a light through hole of the deuterium lamp and the light source outlet are all located on the same horizontal line.

In some embodiments, the refrigerator further comprises a controller and a temperature sensor, wherein the temperature sensor is installed in the darkroom box, and the heat dissipation fan and the temperature sensor are electrically connected with the controller.

In some embodiments, the slit plate further has a third slit and a fourth slit, the first slit, the second slit, the third slit and the fourth slit are uniformly distributed on the slit plate, the first slit and the second slit have the same width, and the second slit, the third slit and the fourth slit have different widths.

In some embodiments, the set of photodiodes includes a first photodiode aligned with the sample channel of the flow cell and a second photodiode aligned with the reference channel of the flow cell.

In some embodiments, the filter is a holmium glass filter.

In some embodiments, the first motor and the second motor are both precision motors.

The utility model discloses following beneficial effect has: the utility model provides a liquid chromatogram's ultraviolet-visible light detector cools down the desuperheating to the light source device through setting up cooling fan, guarantees the holistic stability of detector, further maintains liquid chromatogram system's stability, is provided with the light filter in addition on the second slit, rotates the slit board through first motor, realizes the position exchange of first slit and second slit and light filter combination, realizes the wavelength calibration work of detector. Thereby improving the detection accuracy of the liquid chromatography system as a whole.

Drawings

FIG. 1 is a schematic diagram of the UV-visible detector optical path system of the liquid chromatograph of the present invention;

fig. 2 is a schematic view of the plane structure of the slit plate of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in detail with reference to the accompanying drawings 1-2 in conjunction with the following embodiments.

The utility model provides a liquid chromatogram's ultraviolet-visible light detector, concrete technical scheme is as follows:

the ultraviolet-visible light detector of the liquid chromatogram comprises a main box body, wherein the main box body is a dark box, a heat radiation fan 1, a light source device 2, an M1 reflector 3, a slit plate 4, an M2 reflector 5, a grating 6, an M3 reflector 7, a flow cell 8 and a photodiode group 9 are arranged in the main box body, and the heat radiation fan 1 is arranged towards the light source device 2 and used for reducing the temperature of the light source device 2, facilitating the heat radiation of the light source device and ensuring the stability of a liquid chromatogram system. The slit plate 4 is rotatably arranged through a first motor 41, a first slit 42 and a second slit 43 are formed in the slit plate 4, an optical filter 44 is installed below the second slit 43, the first slit 42 or the second slit 43 is aligned with a focus of the M1 reflector 3 and a focus of the M2 reflector 5, the grating 6 is connected with the second motor through a rotating shaft 61, and a light path of the light source device 2 sequentially passes through the M1 reflector 3, the first slit 42 or the second slit 43, the M2 reflector 5, the grating 6, the M3 reflector 7 and the flow cell 8 and irradiates on the photodiode group 9. The light source device 2 emits a light source to the M1 reflecting mirror 3 and then images the light source on the M2 reflecting mirror 5 through the first slit 42 on the slit plate 4, wherein the slit plate 4 is switched to a combination of the second slit 43 and holmium glass through the rotation of the first motor 41, the combination of the second slit 43 and the holmium glass can be used for calibrating the wavelength, the light passing M2 reflecting mirror 5 reflects the light to the grating 6, the grating 6 is driven by the second motor to rotate through the rotating shaft 61, the wavelength is adjusted through the rotation of the second motor, the grating 6 swings back and forth rapidly, dual-wavelength detection can be achieved, the light is split through the grating 6, and the obtained monochromatic light enters the flow cell 8 through the M3 reflecting mirror 7 and irradiates the photodiode group 9.

In some embodiments, the light source device 2 includes a darkroom box 21, a light source outlet 211 is disposed at one side of the darkroom box 21, a quartz lens 212 is installed on the light source outlet 211, a tungsten lamp 22, a lens 23 and a deuterium lamp 24 are fixedly installed in the darkroom box 21, the lens 23 is disposed between the tungsten lamp 22 and the deuterium lamp 24, and the center points of the tungsten lamp 22 and the lens 23, and the light-passing hole of the deuterium lamp 24 and the light source outlet 211 are all located on the same horizontal line. The utility model discloses a back of body passes through formula deuterium lamp 24, the use wave band scope of tungsten lamp 22 is between 400-changing 900nm, and the use wavelength scope of deuterium lamp 24 is between 200-changing 600nm, and tungsten lamp 22 transmission light assembles the light to deuterium lamp 24's logical unthreaded hole through tungsten lamp 22 lens 23, and tungsten lamp 22 and deuterium lamp 24 emit to M1 speculum 3 through quartz lens 23212.

In some embodiments, the system further comprises a controller (the controller can adopt any type of existing single chip microcomputer) and a temperature sensor, the temperature sensor is installed in the darkroom box 21, and the cooling fan 1 and the temperature sensor are electrically connected with the controller. The deuterium lamp 24 needs to be preheated for a certain time to be stable, the heat dissipation fan 1 is not started when the deuterium lamp is started, when the temperature sensor detects that the temperature in the darkroom box 21 reaches a set value, the controller receives a signal transmitted by the temperature sensor to control the heat dissipation fan 1 to rotate, and the speed of the heat dissipation fan 1 can be regulated through the controller, so that the temperature of the darkroom box 21 provided with the deuterium lamp 24 can be controlled within a required range, the stability of a light source is further realized, and the stability of the signal is further ensured.

In some embodiments, the slit plate 4 further defines a third slit 45 and a fourth slit 46, the first slit 42, the second slit 43, the third slit 45 and the fourth slit 46 are uniformly distributed on the slit plate 4, the first slit 42 and the second slit 43 have the same width, and the second slit 43, the third slit 45 and the fourth slit 46 have different widths. In this way, the slit plate 4 can be rotated by the first motor 41, and the first slit 42, the second slit 43, the third slit 45, or the fourth slit 46 can be freely switched to align with the M1 mirror 3 to receive the transmitted light path, thereby achieving the effect of slit adjustment and improving the resolution precision of light.

In some embodiments, the set of photodiodes 9 comprises a first photodiode aligned to the sample channel of the flow cell 8 and a second photodiode aligned to the reference channel of the flow cell 8. The obtained monochromatic light simultaneously irradiates a sample light channel and a reference light channel of a flow cell 8 through an M3 reflecting mirror 7, two independent photodiodes, namely a first photodiode and a second photodiode, are arranged behind the sample light channel and the reference light channel, the energy of the sample light and the energy of the reference light are detected in real time, and the concentration of the sample is calculated through the Lambert beer law.

In certain embodiments, the filter 44 is a holmium glass filter 44. The characteristic signal of the deuterium lamp 24 is found from the scanned data, and then calibration and correction of the wavelength are performed to ensure the wavelength accuracy. The detector can detect two characteristic spectra of the deuterium lamp 24 without using holmium glass, the wavelengths of the two characteristic spectra are known, and thus the positioning of the wavelengths further realizes the calibration of the wavelengths; the filter 44 is made of holmium glass, the filter 44 can filter some stray light, and the detector can see the other two characteristic spectrums of the deuterium lamp 24, so that the four characteristic spectrums exist by adding the two characteristic spectrums without holmium glass, and the four characteristic spectrums are more accurately positioned.

In some embodiments, the first motor 41 and the second motor are both precision motors. This improves the rotational accuracy of the slit plate 4 and the grating 6.

The above-mentioned only the utility model discloses the feasible embodiment of preferred is not right the utility model discloses a restriction, the utility model discloses also not be limited to the above-mentioned example, technical field's technical personnel the utility model discloses an in the essential scope, change, modification, interpolation or replacement made also should belong to the utility model discloses a protection scope.

Claims (7)

1. The ultraviolet-visible light detector of the liquid chromatogram is characterized by comprising a main box body, the main box body is a dark box, a heat radiation fan, a light source device, an M1 reflector, a slit plate, an M2 reflector, a grating, an M3 reflector, a flow cell and a photodiode group are arranged in the main box body, the heat radiation fan is arranged towards the light source device, the slit plate is rotationally arranged through a first motor, the slit plate is provided with a first slit and a second slit, an optical filter is arranged below the second slit, the first slit or the second slit is aligned with the focus of the M1 mirror and the focus of the M2 mirror, the grating is connected with a second motor through a rotating shaft, and the light path of the light source device sequentially passes through the M1 reflector, the first slit or the second slit, the M2 reflector, the grating, the M3 reflector and the flow cell to irradiate the photodiode group.

2. The uv-vis detector of claim 1, wherein the light source device comprises a darkroom box, a light source outlet is disposed at one side of the darkroom box, a quartz lens is mounted on the light source outlet, a tungsten lamp, a lens and a deuterium lamp are fixedly mounted in the darkroom box, the lens is disposed between the tungsten lamp and the deuterium lamp, and the tungsten lamp, the optical center of the lens, the light-passing hole of the deuterium lamp and the light source outlet are all located on the same horizontal line.

3. The uv-vis detector of claim 2, further comprising a controller and a temperature sensor, wherein the temperature sensor is mounted in the darkroom tank, and the cooling fan and the temperature sensor are both in electrically controlled connection with the controller.

4. The uv-vis detector of claim 1, wherein the slit plate further comprises a third slit and a fourth slit, the first slit, the second slit, the third slit and the fourth slit are uniformly distributed on the slit plate, the first slit and the second slit have the same width, and the second slit, the third slit and the fourth slit have different widths.

5. The uv-vis detector of claim 1, wherein the set of photodiodes comprises a first photodiode aligned with a sample channel of the flow cell and a second photodiode aligned with a reference channel of the flow cell.

6. The uv-vis detector of liquid chromatography according to claim 1, wherein the filter is a holmium glass filter.

7. The uv-vis detector of claim 1, wherein the first motor and the second motor are precision motors.

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